• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2016년도 추계학술발표대회
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• pp.510-513
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• 2016

Detecting of the object in image processing is substantial but it depends on the object itself and the environment. An object can be detected either by its shape or color. Color is an essential for pattern recognition and computer vision. It is an attractive feature because of its simplicity and its robustness to scale changes and to detect the positions of the object. Generally, color of an object depends on its characteristics of the perceiving eye and brain. Physically, objects can be said to have color because of the light leaving their surfaces. Here, we conducted experiment in the aquarium fish tank. Different color of fish robots are mimic the natural swim of fish. Unfortunately, in the underwater medium, the colors are modified by attenuation and difficult to identify the color for moving objects. We consider the fish motion as a moving object and coordinates are found at every instinct of the aquarium to detect the position of the fish robot using OpenCV color detection. In this paper, we proposed to identify the position of the fish robot by their color and use the position data to control the fish robot gathering in one point in the fish tank through serial communication using RF module. It was verified by the performance test of detecting the position of the fish robot.

• Food Science and Biotechnology
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• 제17권3호
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• pp.508-513
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• 2008

Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

• 한국어병학회지
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• 제12권1호
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• pp.66-69
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• 1999

해산어의 참돔이리도바이러스 (RSIV) 감염 상황을 조사하기 위한 진단법으로 중합효소연쇄반응법(PCR)을 확립하고 1998년 여름 통영을 중심으로 대량폐사가 일어난 남해안 지역의 돌돔, 참돔 및 조피볼락을 대상으로 RSIV의 검출을 행한 결과 남해안 전역의 RSIV 감염이 확인되어졌다.

• 수산해양기술연구
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• 제22권4호
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• pp.98-103
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• 1986

The passive acoustic system only has generally used in fish detection. But the passive acoustic system has not been tried in fishing since Freytag has proposed a possibilities of the passive detection of fishes in 1963. This paper describes the .feasible aspects of fish detection by listening of the sound they make. The passive acoustic system accompanied the active acoustic system may expand the range of detection and compensate for lack of capabilities each other, but there are some difficulties in noise rejection because the fre9uency range of ship noises covers the whole range vf biological sounds. The attempt to collect useful informations from underwater would be greatly contributed in fisheries.

• 한국콘텐츠학회논문지
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• 제21권1호
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• pp.552-560
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• 2021

현재 국내 수산 양식업은 스마트화를 추진하고 있지만, 여전히 양식 단계의 많은 과정에서 사람의 주관적인 판단으로 진행되고 있다. 수산 양식업 스마트화를 위해서 선행되어야 할 부분은 양식장 내 물고기들의 상태를 효과적으로 파악하는 것이다. 어류 개체 수, 크기, 이동경로, 이동속도 등을 파악하여 실시간 모니터링 할 수 있게 된다면 사료 자동 급이, 질병유무판단 등 다양한 양식자동화를 진행할 수 있을 것이다. 본 연구에서는 수중 촬영한 어류비디오 데이터를 이용하여 실시간으로 어류의 상태를 파악 할 수 있는 알고리즘을 제안하였다. 어류 객체검출을 위해 딥러닝 기반 최신 객체검출 모델들을 적용하여 검출 성능을 비교 평가 하였고, 검출 결과를 이용하여 비디오내의 연속적인 이미지 프레임에서 어류 객체 ID부여, 이동경로 추적 및 이동속도를 측정할 수 있는 알고리즘을 제안하였다. 제안한 알고리즘은 객체 검출 성능 92%(F1-score 기준)를 보였으며, 실제 테스트비디오 상에서 실시간으로 다수의 어류 객체를 효과적으로 추적하는 것을 확인하였다. 본 논문에서 제안하는 알고리즘을 이용하여 향후 사료 자동 급이, 어류 질병 예측 등 다양한 스마트양식 기술에 효과적으로 활용될 수 있을 것으로 기대한다.

• 한국어병학회지
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• 제9권2호
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• pp.169-176
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• 1996

연어과 어류의 이상유영 원인 바이러스 RVS의 ELISA법에 의한 신속 진단 방법을 개발하였다. 주화세포를 이용한 바이러스 배양액 및 감염 무지개송어의 뇌조직 마쇄액을 사용하여 실험하였다. 바이러스 배양액을 이용한 ELISA법의 검출 감도 조사에서 최소 바이러스 감염가 검출 한계치는 $10^{2.6}$ $TCID_{50}/100{\mu}l$ 이었다. 또한, 인공감염어의 뇌조직 마쇄액 내의 RVS 항원도 검출 되었다. 본 방법은 현장에서의 RVS 감염어 조사에 효과적으로 사용되어질 수 있는 방법으로 생각 되어진다.

• 대한전자공학회논문지SP
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• 제42권5호
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• pp.79-84
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• 2005

일반적인 카메라의 시야는 사람에 비하여 매우 좁기 때문에 큰 물체를 한 화면으로 얻기 힘들며, 그 움직임도 넓게 감시하기에 어려움 점이 많다. 이에 본 논문에서는 어안 렌즈(Fish-Eye Lens)를 사용하여 넓은 시야의 영상을 획득하고 전방향 감시를 위한 투시(perspective) 영상과 파노라마(panorama) 영상을 복원하는 방법을 제시한다. 영상 변환 과정에서 어안 렌즈의 특성으로 인한 해상도 차이를 보완하기 위하여 여러 가지 영상 보간법을 적용하고 결과를 비교하였다. 그리고 ME(Moving Edge) 방법으로 움직임을 검출하여 다중 물체를 추적할 수 있도록 하였다.

• 한국수산과학회지
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• 제43권1호
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• pp.83-88
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• 2010

Acoustic telemetry is a useful method to investigate fish behavior and is widely used to obtain biological information. In this study, the detection ability of a mooring-type acoustic telemetry system and the seasonal changes were studied for survey design and data analysis. The system detection range was examined with an underwater noise model, and seasonal changes were estimated with a ray-tracing program and underwater temperature profile data. The field experiment was conducted with two sets of pingers and six receivers to estimate the difference in detection rate by installation depth and to compare the model estimate. Results indicated that the long-range detection ability of the acoustic telemetry system was significantly affected by underwater temperature. The detection rate rapidly decreased near the sea surface or bottom despite that the near-range Signal to noise ratio was sufficient.

• Fisheries and Aquatic Sciences
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• 제8권3호
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• pp.185-187
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• 2005

We tested the in vivo ability of a DNA chip to detect virus-specific genes from virus-infected olive flounder Paralichthys olivaceus and rainbow trout Oncorhynchus mykiss. Target cDNA was obtained from total RNA of virus infected cell lines by reverse transcription (RT) and was labeled with fluorescent dye (Cy5-dUTP). The results show the successful detection of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) genes in the virus-infected fishes.