• Title/Summary/Keyword: fine line

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Investigation on the SARS-CoV-2 RNA and PM-2.5 inside Public Transportations in Seoul (서울시 대중교통 내 SARS-CoV-2 RNA와 PM-2.5 오염도 실증연구)

  • Seo, Minjeong;Hong, Juhee;Rhee, Hojun;Park, Jinsol;Lim, Hakmyeong;Park, Myung Kyu;Min, Byungchul;Park, Eun Sun;Lee, Sanghoon;Kim, Hanjun;Ha, Kwangtae;Kwon, Seungmi;Shin, Jinho;Lee, Jaein;Hwang, Youngok;Oh, Younghee;Shin, Yongseung
    • Particle and aerosol research
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    • v.18 no.2
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    • pp.23-35
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    • 2022
  • Recently, passengers using public transport are concerned about the effects of COVID-19 and fine dust. Therefore, from February 2020 to February 2021, we investigated whether SARS-CoV-2 RNA was detected even after disinfection in 55 public transportation places visited by confirmed patients in Seoul. 34 air samples and 702 object surface samples were collected and tested with RT-PCR, one surface sample was positive. In addition, preemptive investigations were conducted in 22 subway trains that passengers were being on board at that time. 1,018 preemptive tests were performed, and all were negative. Although PM-2.5 is dangerous in itself, it can be a potential carrier of viruses. It seemed that a solution was needed as one line continuously exceeded the criteria of PM-2.5. Through this study, it is judged that cluster infection in public transportation can be prevented if efforts to reduce the concentration of fine dust, appropriate disinfection management, and personal disinfection such as wearing a mask in public transportations.

Investigating the Effect of Photoinitiator Types and Contents on the Photocuring Behavior of Photocurable Inks and Their Applications for Etching Resist Inks (광개시제 종류 및 함량에 따른 광경화형 잉크의 광경화 특성과 인쇄회로기판용 에칭 레지스트 소재로의 적용성 연구)

  • Bo-Young Kim;Subin Jo;Gwajeong Jeong;Seong Dae Park;Jihoon Kim;Eui-Keun Choi;Myong Jae Yoo;Hyunseung Yang
    • Applied Chemistry for Engineering
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    • v.34 no.4
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    • pp.444-449
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    • 2023
  • As electronic devices become smaller and more integrated, the demand for manufacturing thin, flexible printed circuit boards (FPCBs) has increased. Although FPCBs are conventionally manufactured by a photolithography method using dry film resist, this process is complicated, and the mask is specifically designed to obtain the precision of the desired circuit line width. In this regard, manufacturing FPCBs with fine patterns through the direct printing method of photocurable inks has gained growing attention. Since the manufacturing process of FPCBs is based on the direct printing method that includes etching and stripping processes utilizing acid and basic chemicals, controlling the adhesion strength, the etching resistance, and the strippability of photocured inks has drawn a lot of attention for the fabrication of fine patterns through photocurable inks. In this study, acrylic ink with various types and contents of the photoinitiator was prepared, and the curing behavior was analyzed. Also, the adhesion strength, etching resistance, and strippability were analyzed to evaluate the applicability of developed photocurable etching resist inks.

Plasma Etching Process based on Real-time Monitoring of Radical Density and Substrate Temperature

  • Takeda, K.;Fukunaga, Y.;Tsutsumi, T.;Ishikawa, K.;Kondo, H.;Sekine, M.;Hori, M.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.93-93
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    • 2016
  • Large scale integrated circuits (LSIs) has been improved by the shrinkage of the circuit dimensions. The smaller chip sizes and increase in circuit density require the miniaturization of the line-width and space between metal interconnections. Therefore, an extreme precise control of the critical dimension and pattern profile is necessary to fabricate next generation nano-electronics devices. The pattern profile control of plasma etching with an accuracy of sub-nanometer must be achieved. To realize the etching process which achieves the problem, understanding of the etching mechanism and precise control of the process based on the real-time monitoring of internal plasma parameters such as etching species density, surface temperature of substrate, etc. are very important. For instance, it is known that the etched profiles of organic low dielectric (low-k) films are sensitive to the substrate temperature and density ratio of H and N atoms in the H2/N2 plasma [1]. In this study, we introduced a feedback control of actual substrate temperature and radical density ratio monitored in real time. And then the dependence of etch rates and profiles of organic films have been evaluated based on the substrate temperatures. In this study, organic low-k films were etched by a dual frequency capacitively coupled plasma employing the mixture of H2/N2 gases. A 100-MHz power was supplied to an upper electrode for plasma generation. The Si substrate was electrostatically chucked to a lower electrode biased by supplying a 2-MHz power. To investigate the effects of H and N radical on the etching profile of organic low-k films, absolute H and N atom densities were measured by vacuum ultraviolet absorption spectroscopy [2]. Moreover, using the optical fiber-type low-coherence interferometer [3], substrate temperature has been measured in real time during etching process. From the measurement results, the temperature raised rapidly just after plasma ignition and was gradually saturated. The temporal change of substrate temperature is a crucial issue to control of surface reactions of reactive species. Therefore, by the intervals of on-off of the plasma discharge, the substrate temperature was maintained within ${\pm}1.5^{\circ}C$ from the set value. As a result, the temperatures were kept within $3^{\circ}C$ during the etching process. Then, we etched organic films with line-and-space pattern using this system. The cross-sections of the organic films etched for 50 s with the substrate temperatures at $20^{\circ}C$ and $100^{\circ}C$ were observed by SEM. From the results, they were different in the sidewall profile. It suggests that the reactions on the sidewalls changed according to the substrate temperature. The precise substrate temperature control method with real-time temperature monitoring and intermittent plasma generation was suggested to contribute on realization of fine pattern etching.

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Analysis of Two-Dimensional Pollutant Transport in Meandering Streams (사행하천에서 오염물질의 2차원 거동특성 해석)

  • Oh, Jung-Sun;Seo, Il-Won;Kim, Young-Han
    • Journal of Korea Water Resources Association
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    • v.37 no.12
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    • pp.979-991
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    • 2004
  • In this study, RMA2 and RMA4, the 2-D depth-averaged models, were employed to simulate the two-dimensional mixing characteristics of the pollutants in the natural streams. The velocity and depth were first calculated using RMA2, 2-D hydrodynamic model, and then the resulting flow field was inputted to RMA4, 2-D water quality model, to compute the concentration field. RMA models were verified using the velocity and concentration data measured in S-curved meandering channel. The results showed that the RMA2 model simulated well the phenomenon that the maximum velocity line is located at the Inner bank of meandering channel, and the RMA4 model was well adapted to reproduce the general mixing behavior and the separation of tracer clouds. Comparing model simulations with measured data in the field experiments, RMA2 model simulated well general flow field and tendency that the maximum velocity line skewed toward the outer bank which were found in field experiments. The simulations of RMA4 model showed that the center of the tracer cloud tends to follow the path in which the maximum velocity occurs. In this study, the dispersion coefficients are fine-tuned based on the measured coefficients calculated using field concentration data, and the results show reasonable agreement with predictive equations.

Establishment and Characterization of Multipotent Germ Line Stem Cells (MGSCs) from Neonatal Mouse Testis (신생 생쥐 고환에서 기인한 다분화능 생식줄기세포주의 확립 및 특성 분석)

  • Han, Sang-Chul;Song, Haeng-Seok;Jun, Jin-Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.1
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    • pp.39-48
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    • 2008
  • Objective: The aim of this study was to investigate whether multipotent germline stem cells (MGSCs) can be established from neonatal mouse testis. Methods: Various cells containing MGSCs were collected from neonatal testis of ICR mice and allocated to plates for in vitro culture. After 7 days in culture, the cells were passed to a fresh culture plate and continuously cultured. From the third or fourth passage, the presumed MGSCs were cultured and maintained on mitomycin C-inactivated STO feeder cells. The MGSCs were cultured in a condition where mouse embryonic stem cells (ESCs) are cultured. Characteristics of the MGSCs were evaluated by RT-PCR, immunocytochemistry, alkaline phosphatase activity, karyotyping, and transmission electron microscopy. Results: Two MGSCs lines were established from 9 pooled sets of neonatal testicular cells. MGSCs colonies were morphologically undistinguishable from ESCs colonies and both MGSC lines as well as ESCs expressed undifferentiated stem cell markers, such as Thy-1, Oct-4, Nanog, Sox2 and alkaline phosphatase. Fine structure of undifferentiated MGSCs were similar to those of ESCs and 60% of MGSCs (12/20) had normal karyotype at passage 10. They were able to form embryoid bodies (EBs) and MGSC-derived EBs expressed marker genes of three germ layers. Conclusion: We could establish the MGSCs from neonatal mouse testis and they were differentiated to multipotent lineages of three germ layers. Molecular characteristics of MGSCs were similar to those of ESCs. Our results suggest a possibility that multipotent stem cells derived from testis, the MGSCs, could replace the ESCs in biotechnology and regenerative medicine.

Experimental Study on the Properties of Strength of the No-Fines Concrete (No-Fines Concrete의 강도특성(强度特性)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Kim, Seong Wan;Sung, Chan Yong;Min, Jeong Kie
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.373-383
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    • 1987
  • No-fines concrete is concrete from which the fine aggregate fraction has been omitted. The concrete so formed, consisting only of coarse aggregate, cement, and water, has large voids uniformly distributed through its mass. This study was performed to obtain the basic data which can be applied to the use of no-fines concrete. The data was based on the properties of no-fines concrete depending upon various mixing ratios. The results obtained were summarized as follows. 1. Test result of the consistency, suitable water-cement ratio was increased with the increasing of mixing ratio. 2. At the suitable water-cement ratio, the highest strengths were showed. But it gradually was decreased with the increasing of mixing ratio and strengths are considerably lower than that of conventional portland cement concrete. 3. The relations between compressive and tensile strength were highly singnificant as a straight line shaped. The strength ratio was decreased with the increasing of mixing ratio and considerably lower than of conventional portland cement concrete. 4. Bulk density was decreased with the increasing of the mixing ratio, and was similar to that of the conventional portland cement concrete at mixing ratio 1:4. 5. The relations between strength and bulk density were highly significant as a straight line shaped. The decreasing ratio of strengths was higher than that of bulk density.

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Fine mapping of qBK1, a major QTL for bakanae disease resistance in rice

  • Ham, Jeong-Gwan;Cho, Soo-Min;Kim, Tae Heon;Lee, Jong-Hee;Shin, Dongjin;Cho, Jun-Hyun;Lee, Ji-Yoon;Yoon, Young-Nam;Song, You-Chun;Oh, Myeong-Kyu;Park, Dong-Soo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.92-92
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    • 2017
  • Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan. This disease has also been identified in Asia, Africa, North America, and Italy. Germinating rice seeds in seed boxes for mechanical transplantation has caused many problems associated with diseases, including bakanae disease. Bakanae disease has become a serious problem in the breeding of hybrid rice, which involves the increased use of raising plants in seed beds. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 ($BC_6F_4$) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). SSR markers evenly distributed in the entire rice chromosomes were selected from the Gramene database (http://www.gramene.org), and the polymorphic markers were used for frame mapping of a $BC_5F_5$ resistant line. Here, we developed 168 near-isogenic rice lines (NILs, $BC_6F_4$) to locate a QTL for resistance against bakanae disease. The lines were derived from a cross between Shingwang, a highly resistant variety (indica), and Ilpum, a highly susceptible variety (japonica). The 24 markers representing the Shingwang allele in a bakanae disease-resistant NIL, YR24982-9-1 (parental line of the $BC_6F_4$ NILs), were located on chromosome 1, 2, 7, 8, 10, 11, and 12. Single marker analysis using an SSR marker, RM9, showed that a major QTL was located on chromosome 1. The QTL explained 65 % of the total phenotype variation in $BC_6F_4$ NILs. The major QTL designated qBK1 was mapped in 91 kb region between InDel15 and InDel21. The identification of qBK1 and the closely linked SSR marker, InDel18, could be useful for improving rice bakanae disease resistance in marker-assisted breeding.

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Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo1', a Japonica Rice Mutant Line (남일벼 돌연변이 후대 계통 'Namil(SA)-flo1'의 분질배유 특성에 대한 유전분석)

  • Mo, Young-Jun;Jeung, Ji-Ung;Kang, Kyung-Ho;Lee, Jeom-Sig;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.3
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    • pp.283-291
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    • 2013
  • Rice varieties with suitable flour-making quality are required to promote rice processed-food industry and boost rice consumption in Korea. 'Namil (SA)-flo1' is an advanced mutant line with floury endosperm which shows good flour-making quality under dry-milling process. Genetic analysis was carried out to localize the chromosomal region responsible for the floury endosperm of 'Namil (SA)- flo1'. By using 94 F2 progenies, which were derived from 'Namil (SA)-flo1' ${\times}$ 'Milyang 23', floury grains percentage was investigated as phenotypic data, and genotyping was conducted with 54 SSR markers. Association analysis showed that the target genetic region for floury endosperm is on middle-low region of chromosome 5. Through further association analysis with increased number of SSR markers on chromosome 5, we found that genotypic variation in RM164 explains 79.7% of the variation in floury grains percentage of F2:3 seeds. The floury endosperm locus was localized on 17.7-20.7 Mbp region of chromosome 5 and will be further analyzed for fine mapping and gene identification.

Thermal pointing error analysis of the observation satellites with interpolated temperature based on PAT method (PAT 기반 온도장 보간을 이용한 관측위성의 열지향오차해석)

  • Lim, Jae Hyuk;Kim, Sun-Won;Kim, Jeong-Hoon;Kim, Chang-Ho;Jun, Hyoung-Yoll;Oh, Hyeon Cheol;Shin, Chang Min;Lee, Byung Chai
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.1
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    • pp.80-87
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    • 2016
  • In this work, we conduct a thermal pointing error analysis of the observation satellites considering seasonal and daily temperature variation with interpolated temperature based on prescribed average temperature (PAT) method. Maximum 200 degree temperature excursion is applied to the observation satellites during on-orbit operation, which cause the line of sight (LOS) to deviate from the designated pointing direction due to thermo-elastic deformation. To predict and adjust such deviation, the thermo-elastic deformation analysis with a fine structural finite element model is accomplished with interpolated thermal maps calculated from the results of on-station thermal analysis with a coarse thermal model. After verifying the interpolated temperatures by PAT with two benchmark problems, we evaluate the thermal pointing error.

Microstructural Characterization of Clad Interface in Welds of Ni-Cr-Mo High Strength Low Alloy Steel (Ni-Cr-Mo계 고강도 저합금강 용접클래드 계면의 미세조직 특성 평가)

  • Kim, Hong-Eun;Lee, Ki-Hyoung;Kim, Min-Chul;Lee, Ho-Jin;Kim, Keong-Ho;Lee, Chang-Hee
    • Korean Journal of Metals and Materials
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    • v.49 no.8
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    • pp.628-634
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    • 2011
  • SA508 Gr.4N Ni-Cr-Mo low alloy steel, in which Ni and Cr contents are higher than in commercial SA508 Gr.3 Mn-Mo-Ni low alloy steels, may be a candidate reactor pressure vessel (RPV) material with higher strength and toughness from its tempered martensitic microstructure. The inner surface of the RPV is weld-cladded with stainless steels to prevent corrosion. The goal of this study is to evaluate the microstructural properties of the clad interface between Ni-Cr-Mo low alloy steel and stainless weldment, and the effects of post weld heat treatment (PWHT) on the properties. The properties of the clad interface were compared with those of commercial Mn-Mo-Ni low alloy steel. Multi-layer welding of model alloys with ER308L and ER309L stainless steel by the SAW method was performed, and then PWHT was conducted at $610^{\circ}C$ for 30 h. The microstructural changes of the clad interface were analyzed using OM, SEM and TEM, and micro-Vickers hardness tests were performed. Before PWHT, the heat affected zone (HAZ) showed higher hardness than base and weld metals due to formation of martensite after welding in both steels. In addition, the hardness of the HAZ in Ni-Cr-Mo low alloy steel was higher than that in Mn-Mo-Ni low alloy steel due to a comparatively high martensite fraction. The hardness of the HAZ decreased after PWHT in both steels, but the dark region was formed near the fusion line in which the hardness was locally high. In the case of Mn-Mo-Ni low alloy steel, formation of fine Cr-carbides in the weld region near the fusion line by diffusion of C from the base metal resulted in locally high hardness in the dark region. However, the precipitates of the region in the Ni-Cr-Mo low alloy steel were similar to that in the base metal, and the hardness in the region was not greatly different from that in the base metal.