• Analytical Science and Technology
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• v.22 no.5
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• pp.395-406
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• 2009

The determination of iodine in the spent nuclear fuel and the volatile behavior during its acid dissolution have been studied by NAA(neutron activation analysis) and electron probe microanalysis (EPMA). Simulated spent fuels (SIMFUELs) were dissolved in $HNO_3$(1+1) at $90^{\circ}C$ for 8 hours. The iodine remained in a dissolver solution after dissolution, and that condensed in dissolution apparatus and trapped in the adsorbent by volatilization during the dissolution were determined, respectively. The condensed iodine was recovered by the redistillation with $HNO_3$(1+1) after transfer of the dissolver solution. The iodines in the dissolver and redistilled solution were separated by solvent extraction followed by ion exchange or precipitation method and determined by RNAA (radiochemical neutron activation analysis). The ion exchange column and filtration kit used for the isolation of iodine, which were prepared with a polyethylene tube, were used as an insert in the pneumatic tube for neutron irradiation. The iodine volatilized during the dissolution of SIMFUELs was collected in a trapping tube containing Ag-silica gel (Ag-impregnated silica gel) adsorbent, and the distribution of iodine trapped in the adsorbents were determined by EPMA. The adsorbing characteristics shown with the SIMFUELs were compared with those shown with a real spent fuel from the nuclear power plant.

• Journal of Environmental Impact Assessment
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• v.32 no.6
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• pp.431-436
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• 2023

As the industry develops, the amount of heavy metals flowing into the ecosystem is increasing. Heavy metals are difficult to decompose and remain in the ecosystem for a long time and cause toxicity, which is removed by physicochemical methods such as adsorption, filtration, and chemical precipitation during water treatment. In this study, Alginate bead was selected as a chelating resin for adsorbing and removing heavy metals, and the Jellyfish Extract at Immunity reaction (JEI) were mixed to evaluate the adsorption efficiency of heavy metals accordingly. beads mixed with JEI showed high adsorption efficiency in lead (79-99%) and copper (64-70%) according to the characteristics of Alginate, and low adsorption efficiency in cadmium (25-37%) and zinc (5-6%). Although heavy metal adsorption did not increase in proportion to the content of JEI, 50% and 100% JEI beads showed significant increases. As a result of applying the reaction rate equation, it was found that it was more suitable for the pseudo-secondary reaction equation than the pseudo-first reaction equation.

• The Journal of Internal Korean Medicine
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• v.45 no.4
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• pp.602-614
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• 2024

Objectives: This study investigated the effects of Korean medicine Oryeong-san (五苓散) on blood glucose levels and evaluated its safety in patients with type 2 diabetes. Methods: A total of 87 patients aged 19 years and older, hospitalized for at least three days at the Kyung Hee University Korean Medical Hospital from January 1, 2021, to December 31, 2023 and continuously consumed Oryeong-san for a minimum of three days, were included. Their clinical characteristics and laboratory tests were reviewed retrospectively. The effects of Oryeong-san on fasting (FBS) and 2-hour postprandial (PP2) blood glucose were assessed by comparing the averages of measurements taken before and after the treatment. To evaluate the safety of the herbal extract, liver function test and kidney function test results were compared, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), creatinine (Cr), and estimated glomerular filtration rate (eGFR). Results: The average duration of Oryeong-san consumption was 17.71±26.82 days. Although the FBS decreased and the PP2 slightly increased, these changes were not statistically significant (p>0.05). Safety assessments showed that the average levels of AST decreased, while the level of ALT increased, without statistical significance. The BUN levels also significantly decreased, while Cr and eGFR levels remained stable. Conclusion: This study indicates that Oryeong-san is safe for use in type 2 diabetes patients without significant impact on blood glucose and that it has a positive safety profile.

• Journal of Life Science
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• v.30 no.2
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• pp.137-146
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• 2020

Calcium is one of the important secondary signaling molecules in plant cells. Calcium-dependent protein kinases (CDPK)-the sensor proteins of Ca²⁺ and phosphorylating enzymes-are the most abundant serine/threonine kinases in plant cells. They convert and transmit signals in response to various stimuli, resulting in specific responses in plants. In rice, 31 CDPK gene families have been identified, which are mainly involved in plant growth and development and are known to play roles in response to various stress conditions. However, little is known about the biochemical characteristics of CDPK proteins. In this study, OsCPK11-a CDPK in rice-was partially purified, and its biochemical characteristics were found. Partially purified OsCPK11 from rice seedlings was obtained by three-step column chromatography that involved anion exchange chromatography consisting of DEAE, hydrophobic interaction chromatography consisting of phenyl-Sepharose, and gel filtration chromatography consisting of Sephacryl-200HR. An in vitro kinase assay using partially purified OsCPK11 was also performed. This partially purified OsCPK11 had a molecular weight of 54 kDa and showed a strong hydrophobic interaction with the hydrophobic resin. In vitro kinase assay showed that the OsCPK11 also had Ca²⁺-dependent autophosphorylation activity. The OsCPK11 phosphorylated histone III-S, and the optimum pH for its kinase activity was found to be 7.5~8.0. The native OsCPK11 shared several biochemical characteristics with recombinant OsCPK11 studied previously, and both had Ca²⁺-dependent autophosphorylation activity and favored histone III-S as a substrate for kinase activity, which also had a Ca²⁺-dependence.

• Economic and Environmental Geology
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• v.46 no.1
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• pp.51-61
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• 2013

Researches on the oil recovery enhancement using the nanotechnology has recently been studied in the United States. The previous researches has focused mainly on the flow characteristics of nanoparticles in porous media, and the stability of the nano-emulsion itself. However, the analysis did not deal with the size effects between a nano-emulsion and the pore size which has an important role when nano-emulsion flows in the porous media. In this research, nano-based emulsion was fabricated which is able to be applied for the enhanced oil recovery techniques and its characteristics was analyzed. In addition, in order to identify the characteristics of nano-emulsions flowing through the porous media, the size effect was analysed by filtering test. According to the results, when the emulsion was fabricated, SCA(Silane Coupling Agent) or PVA(Poly Vinyl Alcohol) are added to improve the stability of emulsion. As the ratio of the decane to water increased, the viscosity of emulsion and the droplet size also increased. For the filtering test at the atmospheric conditions, the droplet did not go through the filter; only the separated water from the emulsion was able to be filtered. This phenomenon occurred because the droplet was not able to overcome the capillary pressure. At the filtering test by suction pressure, most of the emulsion was filtered over the filter size of $60{\mu}m$. However, the ratio of filtration was rapidly degraded at less than $45{\mu}m$ filters. This is caused due to deformation and destruction of the droplet by strong shear stress when passing through the pore. The results from the study on the basic characteristic of nano-emulsion and filtering test will be expected to play as the important role for the fabrication of the stable nano-emulsion or the research on the recovery of residual oil in porous media.

• Korean journal of applied entomology
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• v.12 no.3
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• pp.93-107
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• 1973

Garlic (Allium sativum L.) is an important vegetable crop for the Korean people and has long been cultivated extensively in Korea. More recently it has gained importance as a source of certain pharmaceuticals. This additional use has also contributed to the increasing demand for Korean garlic. Garlic has been propagated vegetatively for a long time without control measures against virus diseases. As a result it is presumed that most of the garlic varieties in Korea may have degenerated. The production of virus-free plants offers the most feasible way to control the virus diseases of garlic. However, little is known about garlic viruses both domestically and in foreign countries. More basic information regarding garlic viruses is needed before a sound approach to the control of these diseases can be developed. Currently garlic mosaic disease is most prevalent in plantings throughout Korea and is considered to be the most important disease of garlic in Korea. Because of this importance, studies were initiated to isolate and characterize the garlic mosaic virus. Symptom expression in test plants, physical properties, purification, serological reaction and morphological characteristics of the garlic mosaic virus were determined. Results of these studies are summarized as follows. 1. Surveys made throughout the important garlic growing areas in Korea during 1970-1972 revealed that most of the garlic plants were heavily infected with mosaic disease. 2. A strain of garlic mosaic virus was obtained from infected garlic leaves and transmitted mechanically to Chenopodium amaranticolor by single lesion isolation technique. 3. The symptom expression of this garlic mosaic virus isolate was examined on 26 species of test plants. Among these, Chenopodium amaranticolor, C. quince, C. album and C. koreanse expressed chlorotic local lesions on inoculated leaves 11-12 days after mechanical inoculation with infective sap. The remaining 22 species showed no symptoms and no virus was recovered from them whet back-inoculated to C. amaranticolor. 4. Among the four species of Chtnopodium mentioned above, C. amaranticolor and C. quinoa appear to be the most suitable local lesion test plants for garlic mosaic virus. 5. Cloves and top·sets originating from mosaic infected garlic plants were $100\%$ infected with the same virus. Consequently the garlic mosaic virus is successively transmitted through infected cloves and top-sets. 6. Garlic mosaic virus was mechanically transmitted to C, amaranticolor when inoculations were made with infective sap of cloves and top-sets. 7. Physical properties of the garlic mosaic virus as determined by inoculation onto C. amaranticolor were as follows. Thermal inactivation point: $65-70^{\circ}C$, Dilution end poiut: $10^-2-10^-3$, Aging in vitro: 2 days. 8. Electron microscopic examination of the garlic mosaic virus revealed long rod shaped particles measuring 1200-1250mu. 9. Garlic mosaic virus was purified from leaf materials of C. amaranticolor by using two cycles of differential centrifugation followed by Sephadex gel filtration. 10. Garlic mosaic virus was successfully detected from infected garlic cloves and top-sets by a serological microprecipitin test. 11 Serological tests of 150 garlic cloves and 30 top-sets collected randomly from seperated plants throughout five different garlic growing regions in Korea revealed $100\%$ infection with garlic mosaic virus. Accordingly it is concluded that most of the garlic cloves and top-sets now being used for propagation in Korea are carriers of the garlic mosaic virus. 12. Serological studies revealed that the garlic mosaic virus is not related with potato viruses X, Y, S and M. 13. Because of the difficulty in securing mosaic virus-free garlic plants, direct inoculation with isolated virus to the garlic plants was not accomplished. Results of the present study, however, indicate that the virus isolate used here is the causal virus of the garlic mosaic disease in Korea.

• The Journal of Engineering Geology
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• v.29 no.1
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• pp.51-68
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• 2019

This study performs to evaluate adaptability of seashore filtering type seawater-intake which adapts dua1 filter well alternative for direct seawater-intake. This study varies filter condition of seashore free surface aquifer which is composed of sand layer then installs real size dual filter well and single filter well to evaluate water permeability and proper pumping amount according to filter condition. According to result of step aquifer test, it is analysed that 110.3% synergy effect of water permeability coefficient is happened compare to single filter since dual filter well has better improvement. dual filter has higher water permeability coefficient compare to same pumping amount, this means dual filter has more improved water permeability than single filter. According to analysis result of continuous aquifer test, it is evaluated that dual filter well (SD1200) has higher water permeability than single filter well (SS800) by analysis of water permeability coefficient using monitoring well and gauging well, it is also analysed dual filter has 110.7% synergy effect of water permeability coefficient. As a evaluation result of pumping amount according to analysis of water level dropping rate, it is analysed that dual filter well increased 122.8% pumping amount compare to single filter well when water level dropping is 2.0 m. As a result of calculating proper pumping amount using water level dropping rate, it is analysed that dual filter well shows 136.0% higher pumping amount compare to single filter well. It is evaluated that proper pumping amount has 122.8~160% improvement compare to single filter, pumping amount improvement rate is 139.6% compare to averaged single filter. In other words, about 40% water intake efficiency can be improved by just installation of dual filter compare to normal well. Proper pumping amount of dual filter well using inflection point is 2843.3 L/min and it is evaluated that daily seawater intake amount is about $4,100m^3/day$ (${\fallingdotseq}4094.3m^3/day$) in one hole of dual filter well. Since it is possible to intake plenty of water in one hole, higher adaptability is anticipated. In case of intaking seawater using dual filter well, no worries regarding damages on facilities caused by natural disaster such as severe weather or typhoon, improvement of pollution is anticipated due to seashore sand layer acts like filter. Therefore, It can be alternative of environmental issue for existing seawater intake technique, can save maintenance expenses related to installation fee or damages and has excellent adaptability in economic aspect. The result of this study will be utilized as a basic data of site demonstration test for adaptation of riverside filtered water of upcoming dual filter well and this study is also anticipated to present standard of well design and construction related to riverside filter and seashore filter technique.

• Journal of the Korean Geotechnical Society
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• v.30 no.6
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• pp.23-39
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• 2014

An artificial rainwater reservoir installed in urban areas for recycling rainwater is an eco-friendly facility for reducing storm water effluence. However, in order to recycle the rainwater directly, the artificial rainwater reservoir requires an auxiliary system that can remove non-point source pollutants included in the initial rainfall of urban area. Therefore, the conventional soil filtration technology is adopted to capture non-point source pollutants in an economical and efficient way in the purification system of artificial rainwater reservoirs. In order to satisfy such a demand, clogging characteristics of the sand filter layers with different grain-size distributions were studied with real non-point source pollutants. For this, a series of lab-scale chamber tests were conducted to make a prediction model for removal of non-point source pollutants, based on the clogging theory. The laboratory chamber experiments were carried out by permeating two types of artificially contaminated water through five different types of sand filter layers with different grain-size distributions. The two artificial contaminated waters were made by fine marine-clay particles and real non-point source pollutants collected from motorcar roads of Seoul, Korea. In the laboratory chamber experiments, the concentrations of the artificial contaminated water were measured in terms of TSS (Total Suspended Solids) and COD (Chemical Oxygen Demand) and compared with each other to evaluate the performance of sand filter layers. In addition, the accumulated weight of pollutant particles clogged in the sand filter layers was estimated. This paper suggests a prediction model for removal of non-point source pollutants with theoretical consideration of the physical characteristics such as the grain-size distribution and composition, and change in the hydraulic conductivity and porosity of sand filter layers. The lumped parameter ${\theta}$ related with the clogging property was estimated by comparing the accumulated weight of pollutant particles obtained from the laboratory chamber experiments and calculated from the prediction model based on the clogging theory. It is found that the lumped parameter ${\theta}$ has a significant influence on the amount of the pollutant particles clogged in the pores of sand filter layers. In conclusion, according to the clogging prediction model, a double-sand-filter layer consisting of two separate layers: the upper sand-filter layer with the effective particle size of 1.49 mm and the lower sand-filter layer with the effective particle size of 0.93 mm, is proposed as the optimum system for removing non-point source pollutants in the field-sized artificial rainwater reservoir.

• Journal of fish pathology
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• v.18 no.1
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• pp.67-80
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• 2005

Phosphoinositide-specific phospholipase $C\delta$ $PLC\delta$) plays an important role in many cellular responses and is involved in the production of second messenger. The present study was conducted to obtain the biochemical characteristics of the expressed recombinant $PLC\delta$ in E. coli cloned from Misgurnus mizolepis and partially purified $PLC\delta$ enzymes from liver tissues of M. mizolepis (wild ML-$PLC\delta$). The ML $PLC\delta$ gene was cloned and expressed under the previous report (Kim et al., 2004), and purified the recombinant protein by successive chromatography using $Ni^{2+}$-NTA affinity column and gel iltration FPLC column. The wild ML-$PLC\delta$ protein was solublized with 2 M KCI and purified by successive chromatography on open heparin-Sephagel and analytical TSKgel heparin-5PW. Both the recombinant and wild ML-$PLC\delta$ form of protein showed a concentration-dependent PLC activity to phosphatidylinositol 4,5-bis-phosphate (PIP$_2$) or phosphatidylinositol (PI). Its activity was absolutely $Ca^{2+}$- dependant, which was similar to mammalian $PLC\delta$ isozymes. Maximal PI-hydrolytic activations of recombinant and wild ML- TEX>$PLC\delta$ was at pH 7.0 and pH 7.5, respectively. In addition, the enzymatic activities of recombinant and wild ML-$PLC\delta$ were increased in concentration-dependent manner by detergent, such as sodium deoxycholate SDC), phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The activities decreased in contrast by a polyamine, such as spermine. Western blotting showed that several types of $PLC\delta$ isozymes exist in various organs. Taken together our results, it suggested that the biochemical characteristics of ML-$PLC\delta$ are similar with those of mammalian $PLC\delta1$ and ${\delta}3$ isozymes.

• Korean Journal of Environmental Agriculture
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• v.31 no.4
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• pp.351-358
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• 2012

BACKGROUND: Constructed wetlands for wastewater treatment are vegetated by wetland plants. Wetland plants are an important component of wetlands, and the plants have several roles in relation to the livestock wastewater treatment processes. The objectives of this study were to investigate the growth characteristics and nutrient absorption of water plants in constructed wetlands for treating livestock wastewater. METHODS AND RESULTS: In this study, livestock wastewater treatment plant by constructed wetlands consisted of $1^{st}$ water plant filtration bed, $2^{nd}$ activated sludge bed, $3^{rd}$ vertical flow(VF), $4^{th}$ horizontal flow(HF) and $5^{th}$ HF beds. Phragmites communis TRINIUS(PHRCO) was transplanted in $3^{rd}$ VF bed, Iris pseudoacorus L(IRIPS) was transplanted in $4^{th}$ HF bed and PHRCO, IRIPS and Typha orientalis PRESEL(THYOR) were transplanted in $5^{th}$ HF. Growth of water plants in constructed wetlands were the highest in October. The IRIPS growth was higher than other plant as 264 g/plant in October. The absorption of nitrogen and phosphorus by IRIS were 3.38 g/plant and 0.634 g/plant, respectively. The absorption of K, Ca, Mg, Na, Fe, Mn, Cu and Zn by water plants were higher in the order of IRIPS > THYOR > PHRCO. CONCLUSION(S): The absorption of nutrients by water plants were higher on the order of IRIPS > THYOR > PHRCO in constructed wetlands for treating livestock wastewater.