• 한국정보통신학회논문지
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• 제18권4호
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• pp.1000-1006
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• 2014

본 연구는 스마트폰에서 OTT 서비스 이용이 활성화되고 있는 것에 주목하여 텔레비전 콘텐츠 시청 행위 추적 방법의 설계를 제안한다. 이를 위해 기존 OTT서비스 어플리케이션을 활용하여 시청 맥락의 흐름(flow)과 패턴을 기록하는 로그 파일 기록 어플리케이션을 개발하였다. 본 논문에서 제시한 로그 파일 포맷은 기존의 분단위 시청률 조사방법이 아닌 초단위 측정으로 이용자 스마트폰을 통한 시청 행위와 시청 시간을 정확하고 정밀하게 측정할 수 있었다. 또한 실시간 방송 VOD 광고 콘텐츠 속성 및 플레이 모드에 따라 나타나는 시청 행태를 발생 이벤트에 따라 추적할 수 있었으며, GPS 데이터를 로그 파일 기록과 매칭한 결과 시청의 공간적 맥락을 분석할 수 있었다. 연구 결과를 바탕으로 패널을 이용한 통합시청률 조사 또는 모바일 민속지학(Mobile Ethnography) 에서의 방법론적 활용과 같은 학문적 실무적 의의를 논의하였다.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.91-93
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• 2003

It has been estimated that the equivalent of approximately $US 50 billion has been spent on research on the behavior and fate of pesticides in the environment since Rachel Carson published “Silent Spring” in 1962. Much of the resulting knowledge has been summarized explicitly in computer algorithms in a variety of empirical, deterministic, and probabilistic simulation models. These models describe and predict the transport, degradation and resultant concentrations of pesticides in various compartments of the environment during and after application. In many cases the known errors of model predictions are large. For this reason they are typically designed to be “conservative”, i.e., err on the side of over-prediction of concentrations in order to err on the side of safety. These predictions are then compared with toxicity data, from tests of the pesticide on a series of standard representative biota, including terrestrial and aquatic indicator species and higher animals (e.g., wildlife and humans). The models' predictions are good enough in some cases to provide screening of those compounds which are very unlikely to do harm, and to indicate those compounds which must be investigated further. If further investigation is indicated a more detailed (and therefore more complicated) model may be employed to give a better estimate, or field experiments may be required. A model may be used to explore “what if” questions leading to possible alternative pesticide usage patterns which give lower potential environmental concentrations and allowable exposures. We are currently at a maturing stage in this research where the knowledge base of pesticide behavior in the environmental is growing more slowly than in the past. However, innovative uses are being made of the explosion in available computer technology to use models to take ever more advantage of the knowledge we have. In this presentation, current developments in the state of the art as practiced in North America and Europe will be presented. Specifically, we will look at the efforts of the ‘Focus’ consortium in the European Union, and the ‘EMWG’ consortium in North America. These groups have been innovative in developing a process and mechanisms for discussion amongst academic, agriculture, industry and regulatory scientists, for consensus adoption of research advances into risk management methodology.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.256-263
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• 2006

In the model legume Medicago truncatula, two mutants, sickle and sunn, exhibit morphologically and genetically distinct hypernodulation phenotypes. However, efforts to isolate the single recessive and single semidominant genes for sickle and sunn, respectively, by map-based cloning have so far been unsuccessful, partly due to the absence of clones that enable walks from linked marker positions. To help resolve these difficulties, a new bacterial artificial chromosome (BAC) library was constructed using BamHI-digested genomic fragments. A total of 23,808 clones were collected from ligation mixtures prepared with double-size-selected high-molecular-weight DNA. The average insert size was 116 kb based on an analysis of 88 randomly selected clones using NotI digestion and pulsed-field gel electrophoresis. About 18.5% of the library clones lacked inserts. The frequency of the BAC clones carrying chloroplast or mitochondrial DNA was 0.98% and 0.03%, respectively. The library represented approximately 4.9 haploid M. truncatula genomes. Hybridization of the BAC clone filters with a $C_{0}t-l$ DNA probe revealed that approximately 37% of the clones likely carried repetitive sequence-enriched DNA. An ordered array of pooled BAC DNA was screened by polymerase chain reactions using 13 sequence-characterized molecular markers that belonged to the eight linkage groups. Except for two markers, one to five positive BAC clones were obtained per marker. Accordingly, the sickle- and sunn-linked BAC clones identified herein will be useful for the isolation of these biotechnologically important genes. The new library will also provide clones that fill the gaps between preexisting BAC contigs, facilitating the physical mapping and genome sequencing of M. truncatula.

• The Plant Pathology Journal
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• 제28권4호
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• pp.439-445
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• 2012

The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond formation protein B (dsbB), Clp protease (clp), and polyamine synthase (speD). The chitinase production of the SpeD mutant was restored by the addition of exogenous spermidine or spermine to the bacterial cultures. The speD and clp mutants lost in vitro antifungal activities against plant fungal pathogens. However, the gluP and dsbB mutants showed similar antifungal activities to that of the wild-type. The growth of the mutants in nutrient rich conditions containing chitin was similar with that of the wild-type. However, growth of the speD and gluP mutants was defective in chitin minimal medium, but was observed no growth retardation in the clp and dsbB mutant on chitin minimal medium. In this study, we identified the four genes might be involved and play different role in the production of extracellular chitinase and antifungal activity in L. enzymogenes C-3.

• 대한통합의학회지
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• 제8권2호
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• pp.89-95
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• 2020

Purpose : This study aims to evaluate the correlation of cognitive function, activities of daily living (ADL), and driving performance in stroke hemiplegic patients residing in Korea. Methods : Subjects of the study were 18 stroke hemiplegic patients admitted to hospitals situated in Seoul. A clock drawing test (CDT), a modified Barthel index (MBI), and a virtual reality driving simulator (Eca faros-driving simulator) were used to examine their cognitive function, their ADL ability, and their driving skills, respectively. Results : Driving skills of stroke hemiplegic patients were shown to be associated with the CDT evaluation tool (r=-.777) (p<.001), but they were found to have any correlation with MBI (r=-.022) (p>.05). Additionally, an individual's CDT showed that the driving simulator evaluation result (pass/fail) could be discriminated with a sensitivity of 100.0 %, a specificity of 40.0 %, and an accuracy of 66.7 %. The result confirmed that the CDT is a useful evaluation tool for screening driving ability in people with stroke. But the MBI did not show any significant results (sensitivity of 62.5 %, specificity of 40.0 %, and predicted the results of the simulator with 50.0 % of accuracy) (p>.05). Conclusion : This study shows that cognitive function influences the driving performance in people with stroke. Driving skills of stroke hemiplegic patients are seen to be highly related to CDT. In the field of driving rehabilitation, these findings could be useful for evaluating driving skills relating to CDT. Furthermore, the study results will set a guideline for domestic occupational therapists to use the evaluation tool for assessing driving abilities in people with stroke.

• 한국임상수의학회지
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• 제24권1호
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• pp.46-50
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• 2007

A 7-year-old female, Jindo dog was referred to the Veterinary Teaching Hospital with mild dyspnea and anorexia due to a gunshot trauma. The dog was wounded in the thoracic region 3 days ago. Plain radiographs showed the left 8th rib fracture, interstitial pattern in the left caudal lung field and pleural effusion. Abdominal radiographs showed the lead bullet. Computed tomographs(CT) showed the size of pulmonary contusion, laceration, lung parenchymal injuries, hemothorax and perforation of abdominal wall. The therapeutic plan was based on abnormalities seen on CT scans but not clearly seen in survey radiographs. Thoracic CT significantly provides even more informations compared with the corresponding radiographs in thoracic gunshot trauma. Although thoracic survey radiographs are useful as a screening tool, CT is highly sensitive in detecting thoracic injuries after thoracic trauma and is superior to routine thoracic survey radiographs in visualizing lung contusion, pneumothorax and hemothorax. Therefore, we recommend CT in the initial diagnostic work-up of patients with thoracic injuries and with suspected chest trauma because early and exact diagnosis of all thoracic injuries along with sufficient therapeutic consequences may reduce complications.

• 한국철도학회논문집
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• 제18권2호
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• pp.166-174
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• 2015

직접수요모형은 전통적 4단계 수요예측방법론보다 적은 비용과 시간으로 기본구상단계에 적합한 분석방법이다. 본 연구에서는 기존 읍면동 기준보다 약 1/24 공간적 크기를 가진 소지역(집계구)단위 사회경제지표를 활용하여 지방 광역권 12개 도시철도 노선을 대상으로 직접수요모형을 구축하여 예측수요를 실적자료와 비교 분석하였다. 하지만 통계적 분석에 의존하는 직접수요모형은 역별 특성을 반영하지 못하여 승차인원 예측에 한계가 있으며 본 연구에서는 승차인원에 영향을 미치는 인자를 찾아내고 각 영향인자들의 표준화 및 기준을 제시하였다. 특히 사회적 이슈가 되고 있는 경전철 노선 54개 역을 대상으로 실증분석하여 역 특성 기초자료 수집, 분석, 보정방안에 대해 논의하였다. 경전철 역을 대상으로 제안된 방안의 적용성을 검토하였고 향후 직접수요모형의 활용성을 높일 수 있을 것으로 기대된다.

• Restorative Dentistry and Endodontics
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• 제45권2호
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• pp.17.1-17.10
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• 2020

Objectives: Citation analysis provides a unique insight into how scientific interests and research trends have changed over time. The aim of this study was to report on the 50 top-cited papers in dental stem cell research using the Science Citation Index Expanded provided by the Web of Science database to determine the academic importance of each contribution. Materials and Methods: After the screening, article title and type, total citations and citations per year, publication journal, publication year, first and senior authors, country of origin, institution, and university of reprint author were documented for the 50 top-cited articles in dental stem cell research. Keyword analysis was performed to determine which keywords were most/least popular. Results: Top 50-cited articles were cited between 179 to 2,275 times. The majority of papers were published in 2008 and originated from the United States with the highest contribution from the National Institute of Dental & Craniofacial Research. Journal of Dental Research published the highest number of top-cited articles, followed by Stem Cells and Journal of Endodontics. The greatest number of articles was published by two individual authors, Shi and Gronthos. Among 197 unique keywords, dental pulp stem cells and mesenchymal stem cells were the most frequently used. Thirty-eight of the 50 most cited articles were original articles, and 37 of them were in the field of basic science. Conclusions: Basic science studies in dental stem cell research published in high impact factor journals had the highest citation rates.

• 대한약침학회지
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• 제9권2호
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• pp.39-47
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• 2006

Objective : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods : GRR-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). Results : It has no cytotoxic effects on both HepG2 and SNU484 cells in all concentrations(0.1, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 346. The number of more than twofold down-regulated genes was 9. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.

• Journal of Species Research
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• 제7권3호
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• pp.231-239
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• 2018

While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.