• Title/Summary/Keyword: field resistance

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In Vitro Wheat Immature Spike Culture Screening Identified Fusarium Head Blight Resistance in Wheat Spike Cultured Derived Variants and in the Progeny of Their Crosses with an Elite Cultivar

  • Huang, Chen;Gangola, Manu P.;Kutcher, H. Randy;Hucl, Pierre;Ganeshan, Seedhabadee;Chibbar, Ravindra N.
    • The Plant Pathology Journal
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    • v.36 no.6
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    • pp.558-569
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    • 2020
  • Fusarium head blight (FHB) is a devastating fungal disease of wheat (Triticum aestivum L.). The lack of genetic resources with stable FHB resistance combined with a reliable and rapid screening method to evaluate FHB resistance is a major limitation to the development of FHB resistant wheat germplasm. The present study utilized an immature wheat spike culture method to screen wheat spike culture derived variants (SCDV) for FHB resistance. Mycotoxin concentrations determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) correlated significantly (P < 0.01) with FHB severity and disease progression during in vitro spike culture. Selected SCDV lines assessed for FHB resistance in a Fusarium field disease nursery in Carman, Manitoba, Canada in 2016 showed significant (P < 0.01) correlation of disease severity to the in vitro spike culture screening method. Selected resistant SCDV lines were also crossed with an elite cv. CDC Hughes and the progeny of F2 and BC1F2 were screened by high resolution melt curve (HRM) analyses for the wheat UDP-glucosyl transferase gene (TaUGT-3B) single nucleotide polymorphism to identify resistant (T-allele) and susceptible (G-allele) markers. The progeny from the crosses were also screened for FHB severity using the immature spike culture method and identified resistant progeny grouped according to the HRM genotyping data. The results demonstrate a reliable approach using the immature spike culture to screen for FHB resistance in progeny of crosses in early stage of breeding programs.

Liquefaction Characteristic of Pohang Sand Based on Cyclic Triaxial Test (진동삼축시험을 통한 포항 지역 사질토의 액상화 저항 특성 연구)

  • Hwang, Byongyoun;Han, Jin-Tae;Kim, Jongkwan;Kwak, Tae-Young
    • Journal of the Korean Geotechnical Society
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    • v.36 no.9
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    • pp.21-32
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    • 2020
  • In this study, series of cyclic triaxial tests and shear velocity measurement were conducted using Pohang sand, which was taken from liquefaction observed area, to verify the liquefaction characteristics of Pohang. The cyclic resistance ratio(CRR) was derived based on the test results. A specimen was reconstituted into 40% and 80% relative density conditions and then a series of cyclic triaxial tests and shear-wave velocity measurement were conducted. As a result, the effect of particle distribution and relative density to liquefaction resistance was verified. The liquefaction resistance of Pohang sand was evaluated by comparing with other liquefaction resistance of sands from previous research. In addition, the liquefaction resistance curve from field observation data was used to verify the reliability of results from this study by measured shear-wave velocity.

Detection of Colletotrichum spp. Resistant to Benomyl by Using Molecular Techniques

  • Dalha Abdulkadir, Isa;Heung Tae, Kim
    • The Plant Pathology Journal
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    • v.38 no.6
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    • pp.629-636
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    • 2022
  • Colletotrichum species is known as the major causal pathogen of red pepper anthracnose in Korea and various groups of fungicides are registered for the management of the disease. However, the consistent use of fungicides has resulted in the development of resistance in many red pepper-growing areas of Korea. Effective management of the occurrence of fungicide resistance depends on constant monitoring and early detection. Thus, in this study, various methods such as agar dilution method (ADM), gene sequencing, allele-specific polymerase chain reaction (PCR), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied for the detection of benzimidazole resistance among 24 isolates of Colletotrichum acutatum s. lat. and Colletotrichum gloeosporioides s. lat. The result of the ADM showed that C. gloeosporioides s. lat. was classified into sensitive and resistant isolates to benomyl while C. acutatum s. lat. was insensitive at ≥1 ㎍/ml of benomyl. The sequence analysis of the β-tubulin gene showed the presence of a single nucleotide mutation at the 198th amino acid position of five isolates (16CACY14, 16CAYY19, 15HN5, 15KJ1, and 16CAYY7) of C. gloeosporioides s. lat. Allele-specific PCR and PCR-RFLP were used to detect point mutation at 198th amino acid position and this was done within a day unlike ADM which usually takes more than one week and thus saving time and resources that are essential in the fungicide resistance management in the field. Therefore, the molecular techniques established in this study can warrant early detection of benzimidazole fungicide resistance for the adoption of management strategies that can prevent yield losses among farmers.

Lateral Resistance of Reinforced Light-Frame Wood Shear Walls

  • Hyung Woo LEE;Sang Sik JANG
    • Journal of the Korean Wood Science and Technology
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    • v.51 no.1
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    • pp.58-66
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    • 2023
  • In light-frame timber construction, the shear wall is one of the most important components that provide resistance to lateral loads such as earthquakes or winds. According to KDS (Korea Design Standard) 42 50 10, shear walls are to be constructed using wood-based structural sheathing, with studs connected by 8d nails spaced 150 mm along the edge and 300 mm in the field. Even though small-scale residential timber building can be designed to exhibit seismic resistance using light-frame timber shear walls in accordance with KDS 42 50 10, only the abovementioned standard type of timber shear wall is available. Therefore, more types of timber shear walls composed of various materials should be tested to measure their seismic resistance, and the results should be incorporated into the future revision of KDS 42 50 10. In this study, the seismic resistance of shear walls composed of structural timber studs and wood-based structural sheathing with reinforced nailing is tested to evaluate the effects of the reinforcement. For the nailing reinforcement, shear wall specimens are constructed by applying nail spacings of 75-150 mm and 50-100 mm. For the shear wall specimens with one sheathing and reinforced nailing, the shear strengths are 1.7-2.0 times higher than that of the standard shear wall (nail spacing of 150-300 mm). The shear strength of the shear walls with sheathing on both sides is 2.0-2.7 times higher than that of the standard shear wall.

Effect of in-Plane Magnetic Field on Rashba Spin-Orbit Interaction

  • Choi, Won Young;Kwon, Jae Hyun;Chang, Joonyeon;Han, Suk Hee;Koo, Hyun Cheol
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.394-394
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    • 2013
  • The spin-orbit interaction has received great attention in the field of spintronics, because of its property and applicability. For instance, the spin-orbit interaction induces spin precession which is the key element of spin transistor proposed by Datta and Das, since frequency of precession can be controlled by electric field. The spin-orbit interaction is classified according to its origin, Dresselhaus and Rashba spin-orbit interaction. In particular, the Rashba spin-orbit interaction is induced by inversion asymmetry of quantum well structure and the slope of conduction band represents the strength of Rashba spin-orbit interaction. The strength of spin-orbit interaction is experimentally obtained from the Shubnikov de Hass (SdH) oscillation. The SdH oscillation is resistance change of channel for perpendicular magnetic field as a result of Zeeman spin splitting of Landau level, quantization of cyclotron motion by applied magnetic field. The frequency of oscillation is different for spin up and down due to the Rashba spin-orbit interaction. Consequently, the SdH oscillation shows the beat patterns. In many research studies, the spin-orbit interaction was treated as a tool for electrical manipulation of spin. On the other hands, it can be considered that the Rashba field, effective magnetic field induced by Rashba effect, may interact with external magnetic field. In order to investigate this issue, we utilized InAs quantum well layer, sandwiched by InGaAs/InAlAs as cladding layer. Then, the SdH oscillation was observed with tilted magnetic field in y-z plane. The y-component (longitudinal term) of applied magnetic field will interact with the Rashba field and the z-component (perpendicular term) will induce the Zeeman effect. As a result, the strength of spin-orbit interaction was increased (decreased), when applied magnetic field is parallel (anti-parallel) to the Rashba field. We found a possibility to control the spin precession with magnetic field.

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Development of Smart Multi-function Ground Resistivity Measuring Device using Arduino in Wind Farm (풍력 발전단지내 아두이노를 활용한 스마트 다기능 대지 고유 저항 측정 장치 개발)

  • Kim, Hong-Yong;Yoon, Dong-Gi;Shin, Seung-Jung
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.19 no.6
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    • pp.65-71
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    • 2019
  • Conventional methods of measuring ground resistance and ground resistance field measurement are used to measure voltage drop according to the resistance value of the site by applying current by installing a constant interval of measurement electrode. If the stratified structure of the site site is unique, errors in boundary conditions will occur in the event of back acid and the analysis of the critical ground resistance in the ground design will show much difference from simulation. This study utilizes the Arduino module and smart ground measurement technology in the convergent information and communication environment to develop a reliable smart land resistance measuring device even if the top layer of land is unique, to analyze the land resistance and accumulate data to predict the change in the age of the land. Considering the topographical characteristics of the site, we propose a ground resistance measuring device and its method of measuring ground resistance so that the auxiliary electrode can be installed by correctly positioning the angle and distance in measuring ground resistance. Not only is ground resistance value obtained through electrodes installed to allow accurate ground resistance values to be selected, but it can also be used as a useful material for installing electrical facilities in similar areas. Moreover, by utilizing reliable data and analyzing the large sections of the site, a precise analysis of the site, which is important in ground design as well as construction cost, is expected to be used much in ground facility design such as potential rise.

Verification of Prediction Technique of Wave-making Resistance Performance for a Ship attached with a Vertical Blade (수직날개를 부착한 선박의 조파저항 성능 추정 기법의 검증)

  • Choi, Hee-Jong;Park, Dong-Woo
    • Journal of Navigation and Port Research
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    • v.37 no.1
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    • pp.1-7
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    • 2013
  • In this paper the developed prediction technique of wave-making resistance performance for a ship attached with a vertical blade had been verified. Numerical analysis program as a prediction technique had been developed using the Rankine source panel method and the vortex lattice method(VLM). The nonlinearity of the free surface conditions was fully taken into account using the iterative method and the trim and the sinkage of the ship were also considered in the numerical analysis program. Panel cutting method was applied to get hull surface panels. Numerical computations were carried out for a 4000TEU container carrier and the vertical blade was attached 6 different locations astern. To investigate the validity of the numerical analysis program the commercial viscous flow field analysis program FLUENT was used to obtain the viscous flow field around the ship and the model test was performed. The model test results were compared with the numerical analysis results.

Evaluation of Resistance to the Aphid (Aphis glycines Matsumura) in Soybean Cultivars and Germplasms

  • Kim, Myung Sik;Sung, Mi Kyung;Baek, Woon Jang;Kim, Min Hwan;Chung, Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.365-372
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    • 2012
  • Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.

The Performance of Large-diameter Bored Piles and Large-section Barrettes in Decomposed Geomaterials in Hong Kong

  • Ng Charles W.W.
    • Proceedings of the Korean Geotechical Society Conference
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    • 2006.10a
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    • pp.334-408
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    • 2006
  • In Hong Kong, large-diameter (${\ge}600mm$) bored piles and large-section excavated rectangular barrettes are commonly used to support tall buildings to resist both vertical and horizontal loads. These piles and barrettes penetrate through and may found in saprolitic soils and decomposed rocks. Generally, the design of these large bored piles and barrettes involves considerable amount of uncertainty and design parameters must usually be verified by field tests. In this paper, over 50 full-scale load tests on large-diameter bored piles and over 15 large-section of rectangular barrettes in Hong Kong are reviewed and interpreted critically, in particular the degree of mobilisation of side shear resistance using a mobilization rating (MR) factor and a displacement index (DI) for floating bored piles and barrettes and rock-socketed piles, respectively. The author was heavily involved with many of these load tests. The diameter of the bored piles tested ranges from 0.6m to 1.8m and the depth varies from 12m to 75m. Sizes of barrettes critically reviewed include $2.2m{\times}0.6m,\;2.2m{\times}0.8m,\;2.8m{\times}0.8m\;and\;2.8m{\times}1.0m$ (on plan) and the depth varies from 36m and 63m. Based on these field tests, a new failure load criterion for large-diameter bored piles and barrettes is developed and proposed. The side shear resistance of the bored piles and barrettes is quantitatively analyzed with respect to local displacements, standard penetration tests, unconfined compressive strength (UCS) for rock sockets and using the effective stress principle. In addition, the effects of construction including post-grouting, construction time, side scraping and excavation tools on side shear resistance are investigated and reported.

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Plasmid Profiles of Pseudomonas syringae pv. syringae Isolated from Kiwifruit Plants in Korea and the Copper Resistance Determinant (우리나라에서 분리된 참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 플라스미드와 Cu 저항성 유전자)

  • Park, So-Yeon;Han, Hyo-Shim;Lee, Young-Sun;Koh, Young-Jin;Shin, Jong-Sup;Jung, Jae-Sung
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.337-340
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    • 2007
  • Pseudomonas syringae Pv. syringae is a causal agent of bacterial blossom blight of kiwifruit in Korea. Eleven strains of the pathogen were isolated from different kiwifruit orchards in Korea and the plasmid profiles were obtained by pulsed-field gel electrophoresis. They could be clustered into six groups according to the number and size of plasmids. The number of plasmids per strain and size of these plasmids ranged from 0 to 4 and from 22 to 160 kb, respectively. Among them, four strains belonging to Group III which harbored two plasmids were resistant to copper sulfate. Southern blot hybridization of the plasmid DNA indicated that the copper resistance determinant was carried on a 48 kb plasmid.