• The Korean Journal of Ecology
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• 제11권2호
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• pp.65-76
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• 1988

A number of laboratory and field experiments have performed with aqueous extracts from fresh leaves, fallen leaves and roots of Pinus rigida in order to investigae the effetct of the pine tree. The results obtained found its inhibitive effect on the germination and growth of tested species. Though relative germination ratio of the species was somewhat lower than the control plot, severe inhibition was observed in relative dry weight ration, These results were more obvious in aqueous extracts than in the case of leachates and similar results were also confirmed by field bioassays on the forest soil of P. rigida. Therefore, to find out the inhibitory sybstancesm 14 different kinds of KDICical substances were isolatd from the leaved of the tree by chromato-graphy and nist if them were confirmed to be phenoic compounds, The growth of lettuce was inhibition was observed in the concentration of less than 5X10-3M soluton. Phenolic compounds are assumed the substance related to the allelopathic effect of P. rigida.

• Journal of Forest and Environmental Science
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• 제34권5호
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• pp.388-394
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• 2018

An efficient and reproducible protocol for in vitro germination of seeds of Couroupita guianensis has been developed in the present study. Couroupita guianensis is a medicinally important tree and categorized as threatened plant species by the IUCN. The seeds were surface sterilized using 0.1% $HgCl_2$ and inoculated on MS (Murashige and Skoog) and Woody Plants (Llyod and McCown) medium. The seeds with partially removed seed coat responded better (100%) on half strength MS medium as compared to the full (51%), one-fourth strength MS (79%) and the WP medium. Half strength MS medium conjunct with $2.0mg{\cdot}L^{-1}$ indole-3 butyric acid (IBA) was found best among the different types of auxins and cytokinins tested for seed germination, as all the seeds germinated on this medium combination. The seedlings were successfully hardened in the greenhouse and restored in the field with 83% survival rate. The protocol can be used as conservation measure for large scale multiplication of this threatened forest tree species.

• 농업생명과학연구
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• 제44권3호
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• pp.1-6
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• 2010

The influence of sodium hypochlorite and ethephon on the germination of narrow-leaved purple coneflower (Echinacea angustifolia) was investigated. Treatment of seeds with ethephon (1 mM) for 2 h followed by soaking in 0.525% sodium hypochlorite greatly increased germination (>90%). The treatment of seed with a combination of sodium hypochlorite and ethephon shortened number of days required to reach 50% of the final germination ($T_{50}$) from 4.2 days in control to 1.1 days. Seedlings grown from ethephon-treated seeds had shorter and thicker hypocotyls and roots with higher dry weights compared to the control. The same trend was observed during seedling emergence from soil. Ethephon treatment longer than 2 h resulted in weaker seedlings, probably due to leaf senescence. Anthocyanin content in seedling leaves increased linearly from 0.04 mg/g fresh weight in control to 3.72 mg/g fresh weight in 24 h treatment as the time of seed exposure to ethephon increased. Seed treatment with bleach and ethephon may well be practiced to facilitate the establishment of E. angustifolia in the field.

• 원예과학기술지
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• 제35권2호
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• pp.220-231
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• 2017

Grafting using a pumpkin (Cucurbita sp.) rootstock is an effective way to improve cucumber (Cucumis sativus) resistance to a combination of chilling and salinity stresses. We evaluated the tolerance of 15 pumpkin cultivars to chilling, salinity, and combined stresses at the germination and seedling stages. Selected plant characteristics, including germination rate, germination potential, germination index, plant height, stem thickness, fresh weight, and dry weight, were analyzed. We used the unweighted pair group method with arithmetic mean for cluster analyses to determine the stress tolerance levels of the pumpkin cultivars. The 15 cultivars were divided into three clusters: tolerant, moderately tolerant, and susceptible to stress treatments. The stress tolerances of all cultivars were variable in the germination and seedling stages, and most cultivars were not tolerant to individual treatments of chilling or salinity stresses at both stages. These results suggest that identifying suitable cultivars for use as rootstock during cucumber grafting should involve the evaluation of stress tolerance during different growth stages. Additionally, cultivars tolerant to chilling stress may not be tolerant to salinity stress; therefore, the choice of pumpkin rootstock should depend on where the grafted plant will be grown. Cultivars tolerant to a combination of chilling and salinity stresses may be useful as rootstock for cucumber grafting. Our findings may serve as reference material for choosing appropriate pumpkin rootstocks for cucumber grafting.

• 한국약용작물학회지
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• 제8권2호
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• pp.129-133
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• 2000

야생약용 식물인 선학초의 적정 종자처리 방법을 구명하고자 실시한 몇가지 처리에 따른 발아율 및 파종후의 주요생육을 요약하면 다음과 같다. 1. 적정 저온층적기간은 20일로 나타났으며 발아적온은 $20^{\circ}C$, 발아율은 86%이었다. 2. 저온층적처리는 모든 온도에서 발아율을 향상시켰으며, 여타처리에 비하여 발아기는 $26{\sim}32$일 단축시켰으며, 발아율은 20% 이상 높았다. 3. 유수, 저온, $GA_3$, NaOCl의 효과는 적었으며 온도가 높을수록 발아율의 감소가 크게 나타났다. 4. 유묘의 생육은 저온층적처리가 여타처리에 비하여 초장은 $3{\sim}4cm$, 엽수는 1매 증가하였으며, 수확기 생육은 엽수의 증가폭이 커서 33매로 여타처리에 비하여 10매 증가하였다. 5. 개화기는 저온층적처리가 여타처리보다 5일 빠른 6월 18일이었다. 발아기간의 단축에 의한 생육기간의 연장에 의한 것으로 판단된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.

• 한국산림과학회지
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• 제84권1호
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• pp.87-96
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• 1995

새 순(筍)이나 수피(樹皮), 열매 등(等) 식(食) 약용(藥用)으로 이용(利用)하고 있는 화살나무를 비롯한 4수종(樹種)의 종자(種子)에 대하여 포지(圃地) 발아율(發芽率)을 높이는 방법(方法)을 알아보기 위행 화살나무, 차나무, 초피나무의 종자(種子)는 10월(月) 중순(中旬)에서 11월(月) 중순(中旬) 사이에 채취(採取)하여 정선(精選)한 후(後) Pon-Pon, $H_2O_2$, $GA_3$, 종피파쇄(種皮破碎) 등(等)으로 처리(處理)하여 노천매장(露天埋藏)(119일(日)-134일(日))을 하였다가 3월(月) 29일(日) 시험포지(試驗圃地) 파종(播種)하였으며, 남천 종자(種子)는 11월(月) 하순(下旬에) 채취(採取)하여 Growth chamber에서 저장온도(貯藏溫度)($4^{\circ}C$, $25^{\circ}C$, $32^{\circ}C$), 저장기간(貯藏期間)(7일(日), 15일(日), 21일(日))별(別)로 처리(處理)한 후(後) 유리온실(溫室) 내(內)의 파종상자(播種箱子)($55{\times}45{\times}15cm$)에 파종(播種)하여 보관(保管)하던 중(中) 5월(月) 초(初)부터는 노지(露地)에서 관리(管理)하였다. 수종별(樹種別), 종자(種字) 발아촉진처리별(發芽促進處理別)로 발아(發芽) 생장(生長)한 묘목(苗木)을 파종(播種)한 당년(當年) 10월(月) 중순(中旬)에 굴취(掘取)하여 발아율(發芽率)과 유묘(幼苗) 생장(生長)을 조사(調査) 분석(分析)하였다. 종자(種子) 발아촉진처리별(發芽促進處理別) 포지(圃地) 발아율(發芽率)에서 화살나무는 $GA_3$ 처리(處理) 후(後) 노천매장(露天埋藏)한 처리구(處理區)에서 당년(當年) 발아율(發芽率) 67.1%로 일반(一般) 노천매장(露天埋藏) 처리구(處理區) 18.4%보다 높은 발아율(發芽率)을 보여 유의적(有意的)인 발아촉진(發芽促進) 효과(效果)가 인정(認定)되었다. 차나무 종자(種子)는 종피파쇄(種皮破碎)나 $H_2O_2$ 처리(處理) 후(後) 노천매장(露天埋藏)한 처리구(處理區)와 일반(一般) 노천매장처리구(露天埋藏處理區) 간(間)에 뚜렷한 차이(差異)를 보이지 않아 노천매장(露天埋藏) 만으로도 충분한 발아촉진(發芽促進) 효과(效果)가 인정(認定)되었다. 초피나무 종자(種子)의 경우 Pon-Pon 처리(處理) 후(後) 노천매장(露天埋藏)한 처리구(處理區)에서 당년(當年) 발아율(發芽率) 80.3%로 일반(一般) 노천매장(露天埋藏)한 처리구(處理區)의 12.4% 보다 유의적(有意的)인 차이(差異)가 있었다. 남천 종자(種子) $32^{\circ}C$에서 7일간(日間) 저장(貯藏)한 처리구(處理區)가 $4^{\circ}C$에서 7일간(日間) 저장(貯藏)한 처리구(處理區)보다 5개월(個月) 정도 조기(早期)에 발아(發芽)되면서 발아율(發芽率)도 높게 나타났다. 포지(圃地)에 파종(播種)한 종자(種子)의 발아최성기(發芽最盛期)는 화살나무(32일(日))>초피나무(49일(日))>차나무(83일(일))>남천(87일(日)) 순(順)이었다. 포지(圃地)에서 발아(發芽)한 실생묘목(實生苗木) 생장량(生長量)은 종자(種子) 발아촉진처리(發芽促進處理)의 효과(效果)로 발아(發芽)가 빠른 처리구(處理區)에서 양호(良好)하였다. 실생묘(實生苗)의 연(年) 생장시간(生長時間) 중(中)에서 가장 왕성(旺盛)한 생장(生長)을 보인 최대생장(最大生長) 기간(期間)은 화살나무는 종자발아(種子發芽) 후(後) 72일(日) 경(傾)인 6월(月) 하순(下旬)부터 7월(月) 중순(中旬)사이이고, 초피나무는 59일(日)째인 6월(月) 하순(下旬)부터 7월(月) 중순(中旬) 사이였으며 차나무는 54일(日)째인 8월(月) 중순(中旬)부터 9월(月) 중순(中旬)사이로 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.334-334
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.53-53
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• 한국환경복원기술학회지
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• 제3권4호
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• pp.60-73
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• 2000

The purpose of the study was to analyze the feasibility of the alluvial deposit deposited on upper terrace in Han River as a substitute for conventional plant soil. For this purpose, the soil characteristics were analyzed, and germination and growth rate of pansy and marigold for 75 days were investigated. Soil contamination level of all the samples, except mineral oil, was analysed under the legal contamination level, while some mineral oil was detected in almost samples at 2.0~32mg/kg. The measures of the soil texture (sandy loam or loam), organic matter (2.5~5.5%), available phosphate (22~98mg/kg), exchangeable cation of K (0.5~1.1cmol/kg), Ca (0.9~9.6cmol/kg), Mg (0.1~0.7cmol/kg), Na (0.7~3.1cmol/kg), CEC (3.1~24.3cmol/kg) were identified as not worse than those of conventional planting soil. But the pH (5.1~5.3) was detected slightly lower than the range (5.5~6.9) of average domestic field soil. The germination rate of pansy in alluvial deposit was lower than that of fertile field or paddy field soil, while the growth rate of pansy for 75 days in alluvial deposit was as good as that of the compared fertile soils. But the germination rate and growth rate of marigold in alluvial deposit were much poorer than those of marigold in compared fertile soils. Consequently, the feasibility of alluvial deposit as a substitute for planting soil was evaluated to be high.