• Title/Summary/Keyword: fiber analysis

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Studies for Component Analysis and Antioxidative Evaluation in Acorn Powders (도토리 가루의 성분분석과 항산화능 평가)

  • Shim, Tae-Heum;Jin, Ying-Shan;Sa, Jae-Hoon;Shin, In-Cheol;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.800-803
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    • 2004
  • Chemical components and physiological activities of acorn powders were investigated to develop functional food. Proximate components were 87.29% crude fiber, 1.18% crude fat, 0.84% crude protein, and 0.12% crude ash. Potassium was most predominant mineral, followed by phosphorus, calcium, magnesium, and sodium. Contents of unsaturated fatty acids, such as oleic, linoleic, and linolenic acids, were higher than those of saturated fatty acids. Water and 75% ethanol extracts of acorn powders showed higher absorbency at 285 nm. Water and 75% ethanol extracts exhibited antioxidative activity with $IC_{50}$ of 19.0 and $21.4\;{\mu}g/mL$, respectively, indicating they are the major biological component in acorn powders. Results suggest water extract of acorn can be used as new material for natural antioxidant and functional food.

Purinergic regulation of calcium signaling and exocytosis in rat prostate neuroendocrine cells

  • Kim, Jun-Hee;Kim, Mean-Hwan;Koh, Duk-su;Park, So-Jung;Kim, Soo-Jung;Nam, Joo-Hyun;Lee, Jee-Eun;Uhm, Dae-Yong;Kim, Sung-Joon
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.54-54
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    • 2003
  • Prostate gland contains neuroendocrine cells (PNECs) are playing important roles in physiological and pathophysiological processes of the prostate gland. Here, we investigated the role of purinoceptors in PNECs freshly isolated from rat ventral prostate (RPNECs) that show immunoreactivity to chromogranin A. Fura-2 ratiometry revealed that ATP evokes both fast Ca$\^$2+/ influx and store Ca$\^$2+/ release in RPNECs. A whole-cell patch clamp study demonstrated fast inactivating cationic current activated by ATP or by ${\alpha}$,${\beta}$-MeATP, which was blocked by ATP-TNP. The activation of P2X inward current was tightly associated with a sharp increase in [Ca$\^$2+/]$\sub$c/. The presence of P2X1/3 subtypes were proved by RT-PCR analysis. For the stored Ca$\^$2+/ release, ATP and UTP showed similar effects, suggesting the dominant role or P2Y2 subtypes, also confirmed by RT-PCR. Both P2X (${\alpha}$,${\beta}$-MeATP) and P2Y (UTP) stimulation induced changes in the cell morphology (initial shrinkage and blob formation on the surface) reversibly. Exocytotic membrane trafficking events were monitored with the membrane-bound fluorescent dye, FM1-43 using confocal microscopy. In spite of the similar Ca$\^$2+/ responses, UTP was far less effective in triggering exocytosis than ${\alpha}$,${\beta}$ -MeATP. Since serotonin is reportedly stored in the secretory granule of PNECs, we directly examined whether the aforementioned agonists elicit release of serotonin using carbon fiber electrode-amperometry. In accordance with the results of FM1 -43 experiments, ${\alpha}$,${\beta}$-MeATP efficiently evoke serotonin secretion while not with UTP. In summary, the P2X-mediated Ca$\^$2+/ influx plays crucial roles in the exocytosis of RPNECs. Although a global increase in [Ca$\^$2+]$\sub$c/ might be related with the morphological changes, a sharp rise of [Ca$\^$2+/]$\sub$c/ in the putative sub-plasmalemmal ‘microdomains’ might be a decisive factor for the exocytosis.

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Quality Properties of Jeung-pyun with Added Withprickly Pear (Cheonnyuncho) Powder (천년초(Opuntia humifusa) 열매 분말 첨가 증편의 품질 특성)

  • Cho, Eun-Ja;Kim, Min-Jong;Choi, Won-Seok
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.6
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    • pp.903-910
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    • 2007
  • In order to make acquire a potential use for Opuntia humifusa as a natural functional food material, this study was performed to determine the quality properties of Jeung-pyun made with added Opuntia humifusa, or prickly pear powder. According to an analysis of its major components, we found that the prickly pear powder consisteds of nitrogen-free extracts (71.85%) and crude fiber (11.20%). Greater additions of prickly pear powder had resulted in significantly lower pH in the of Jeung-pyun. According to measurements on the degree of Jeung-pyun gelatinization, by means of ${\beta}$-amylase, greater additions of prickly pear powder led to the higher levels of isolated maltose, indicating that the gelatinization degree of the Jeung-pyun became higher. Also, samples with higher concentrations of prickly pear powder had a tendency toward lower water content, which allowed us to expect a longer storage duration for the Jeung-pyun. In the textural property tests the Jeung-pyun that had less hardness and greater adhesiveness (p<0.05) than the control group as the content of prickly pear powder became higher. Also, the Jeung-pyunhad lower gumminess and chewiness than the control group as the content of prickly pear powder became higher. Therefore, it is possible to prepare relatively soft Jeung-pyun using prickly pear powder. For the color differences of the Jeung-pyun samples, lower L- values, and higher a- and b -values (p<0.05) presented as the addition level of prickly pear powder became higher. According to SEM observations of the Jeung-pyun, the added prickly pear powder addition groups generally showed a smaller and more inconsistent pore size, but higher porosity, than the control group. According to sensory analyses of the Jeung-pyun, the P2 group scored highest for color item, and the P4 group generated the fermented scent. Higher additions content of prickly pear powder led to the lower score, but higher scores for adhesiveness. Finally, the P2 group achieved the highest score for overall taste.

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A Study on the Dyadic Sorting method for the Regularization in DT-MRI (Dyadic Sorting 방법을 이용한 DT-MRI Regularization에 관한 연구)

  • Kim, Tae-Hwan;Woo, Jong-Hyung;Lee, Hoon;Kim, Dong-Youn
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.47 no.4
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    • pp.30-39
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    • 2010
  • Since Diffusion tensor from Diffusion Tensor Magnetic Resonance Imaging(DT-MRI) is so sensitive to noise, the principle eigenvector(PEV) calculated from Diffusion tensor could be erroneous. Tractography obtained from PEV could be deviated from the real fiber tract. Therefore regularization process is needed to eliminate noise. In this paper, to reduce noise in DT-MRI measurements, the Dyadic Sorting(DS) method as regularization of the eigenvalue and the eigenvector is applied in the tractography. To resort the eigenvalues and the eignevectors, the DS method uses the intervoxel overlap function which can measure the overlap between eigenvalue-eigenvector pairs in the $3\times3$ pixel. In this paper, we applied the DS method to the three-dimensional volume. We discuss the error analysis and numerical study to the synthetic and the experimental data. As a result, we have shown that the DS method is more efficient than the median filtering methods as much as 79.97%~83.64%, 85.62%~87.76% in AAE, AFA respectively for the corticospinal tract of the experimental data.

Development of a Personal Compound Stimulus Device for Skin-care (개인용 피부미용 복합자극기 개발)

  • Lee, Jeon;Kim, Chi-Hyun;Chung, Geum-Hee
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.49 no.1
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    • pp.12-19
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    • 2012
  • Recently, the market of skin-care device has been steadily growing up. In this paper, we tried to develop a personal compound stimulus device more competitive than existing products. As the compound stimulus, biochemical stimulus of herbal extraction fluid, thermal stimulus of plate-shaped carbon fiber heater, and optical stimulus of near infrared LED were selected. By some evaluation tests, the thermal stimulation part and the optical stimulation part were found to be developed properly. Additionally, the efficacy of the mixed stimulus of thermal and optical stimulation was tested in C2C12 mouse myoblast. Through RT-PCR analysis, it was found that, by the developed compound stimulus, the expression of collagen I mRNA and collagen III mRNA increased by 4.9 and 1.3 times respectively.

Component Analysis and Antioxidant Activity of Aster koraiensis Nakai (벌개미취의 함유성분 분석과 항산화 활성)

  • Shin, Eon Hwan;Park, Sung Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.1
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    • pp.74-79
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    • 2014
  • The purpose of this study was to determine the efficacy of Aster koraiensis Nakai as a natural health food source. To accomplish this, the general and antioxidative contents of A. koraiensis were measured. Total contents of carbohydrates, crude protein, crude lipid, and ash were 72.15%, 13.49%, 5.09%, and 9.27%, respectively. Caloric content of A. koraiensis was 349.70 kcal, while total dietary fiber was 49.79%. Total proteins consisted of 18 different kinds of amino acids. Contents of essential and non-essential amino acids were 4.6 and 5.5 g/100 g, respectively. Regarding mineral contents, P was the most abundant mineral, followed by K, Ca, and Na. Therefore, A. koraiensis is an alkali material. Total phenol contents of the hot water and 80% ethanolic extracts of A. koraiensis were $87.7{\pm}5.01$ and $112.4{\pm}3.41$ mg GAE/g, respectively. Total flavonoid contents of the hot water and 80% ethanolic extracts were $86.6{\pm}3.71$ and $95.1{\pm}8.00$ mg RE/g, respectively. The DPPH radical-scavenging activity and reducing power of the 80% ethanolic extract of A. koraiensis were higher than those of the water extract. Therefore, the general nutrients and antioxidant bioactive materials in A. koraiensis are potential materials for health foods.

Effects of Stabilized Rice Bran on Obesity and Antioxidative Enzyme Activity in High Fat Diet-induced Obese C57BL/6 Mice (안정화 미강이 고지방 식이로 유도된 C57BL/6 Mice의 비만과 항산화 효소 활성에 미치는 영향)

  • Kim, Ja-Young;Shin, Malshick;Heo, Young-Ran
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.8
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    • pp.1148-1157
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    • 2014
  • Rice bran (RB), a by-product obtained during polishing of un-milled rice, contains a large quantity of essential nutrients such as minerals, vitamins, fiber, amino acids, and antioxidants. In this study, the anti-obesity effects of stabilized RB addition as a food material in a diet-induced obese animal model were investigated. For the analysis, a total of 32 mice were randomly divided into four groups: normal diet group (ND, n=8), high-fat diet group (HFD, n=8), 20% RB added high fat diet group (HFRB20, n=8), and 40% RB added high fat diet group (HFRB40, n=8). The animals were sacrificed after 7 weeks. Body weight gain, various adipose tissue weights, blood glucose concentration, and serum leptin level of the HFRB40 group were found to be significantly lower than those of the HFD group. Moreover, antioxidant enzyme activity of the HFRB40 group was significantly higher than that of the HFD group. However, the HFRB20 group did not show significantly different values. The results of this study show that RB (40%) addition reduces body weight gain and improves obesity-related parameters. These results suggest RB could be considered as a functional additive material for the manufacture of anti-obesity products, and 40% RB addition would be the physiologically effective level.

Qualities of Bread and Changes in Phytic Acid during Breadmaking with Whole Wheat Flour (전립분 첨가빵의 품질과 제빵 과정 중 Phytic Acid 변화)

  • 김영호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.779-785
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    • 1996
  • The qualities of bread and change of phytic acid during breadmaking with whole wheat flour were investigated. The ratios of ash contents in wheat flour and whole wheat flour were 0.41% and 1.57%, respectively. The ratios of fiber contents in wheat flour and whole wheat flour were 0.14% and 1.83%, respectively. In amino acid analysis, glutamic acid was determined to be 32~36g/100g protein, which was the highest. Lysine, glycine, arginine and aspartic acid were higher in whole wheat flour than those of wheat flour. Proline, glutamic acid, and phenylalanine were higher in wheat flour than those of whole wheat flour. The ratio of phytic acid content in wheat flour and whole wheat flour was 0.312% and 0.734%, respectively. The content of phytic acid during beadmaking was decreased approximately 65% after proofing, while this was almost constant in the process of oven baking. The content of phytic acid in bread with 3% yeast had less hydrolysis than that in bread with 5% yeast during breadmaking. The phytic acid content in the 0.1% yeast food was decreased more than the 0, 0.3, and 0.5% yeast food groups. As the amount of whole wheat flour increased, the volume of bread was decreased, and color became dark. The sensory evaluation was showed the quality of bread to be the highest when the amounts of coarse whole wheat flour and fine whole wheat flour was 20% and 30%, respectively. Though the amount of coarse whole wheat flour and fine whole wheat flour were increased up to 30% and 50%, respectively, external characteristics of bread was remained in normal.

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Feasibility of Bladder Compression Molded Prepreg as Small Wind Turbine Blade Material (소형 풍력 터빈 블레이드 재료로서 블래더 가압 방식 몰드 성형 프리프레그의 타당성)

  • Yi, Bo-Gun;Seo, Seong-Won;Song, Myung-Ho
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.33 no.2
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    • pp.95-101
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    • 2020
  • The wind turbine blades should be designed to possess a high stiffness and should be fabricated with a light and high strength material because they serve under extreme combination of lift and drag forces, converting kinetic energy of wind into shaft work. The goal of this study is to understand the basic knowledge required to curtail the process time consumed during the construction of small wind turbine blades using carbon fiber reinforced polymer (CFRP) prepeg composites. The configuration of turbine rotor was determined using the QBlade freeware program. The fluid dynamics module simulated the loads exerted by the wind of a specific speed, and the stress analysis module predicted the distributions of equivalent von Mises stress for representing the blade structures. It was suggested to modify the shape of test specimen from ASTM D638 to decrease the variance in measured tensile strengths. Then, a series of experiments were performed to confirm that the bladder compression molded CFRP prepreg can provide sufficient strength to small wind turbine blades and decrease the cure time simultaneously.

Analysis of Temperature dependent Thermal Expansion Behavior of $\textrm{SiC}_\textrm{p}/\textrm{Al}_2\textrm{O}_{3f}/\textrm{Al}$ Composites ($\textrm{SiC}_\textrm{p}/\textrm{Al}_2\textrm{O}_{3f}/\textrm{Al}$ 복합재료의 온도에 따른 열팽창 특성 해석)

  • 정성욱;남현욱;정창규;한경섭
    • Composites Research
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    • v.16 no.1
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    • pp.1-12
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    • 2003
  • This study developed SiC$_{p}$/A1$_2$O$_3$$_{f}$/Al composites for electronic packaging to which reinforcements were added with the volume fractions of 49%, 56% and 63% by the squeeze casting method. 0.8 wt. % of the inorganic binder as well as the A1$_2$O$_3$ fiber and SiC Particles with the volume fraction of 1:10 were added to the composites, which were produced in the newly designed mold. For the produced SiC/Al composites, the CTEs (coefficients of thermal expansion) were measured from 30 to 300 and compared with the FEM numerical simulation to analyze the temperature dependent properties. The experiment showed the CTEs of SiC$_{p}$/A1$_2$O$_3$$_{f}$/Al composites that were intermediate values of those of Rule of Mixture and Turner's Model. The CTEs were close to Turner's Model in the room temperature and approached the Rule of Mixture as the temperature increases. These properties analyzed from the difference of the average stress acting between the matrix and the reinforcements proposed in this study.