• Title/Summary/Keyword: fiber analysis

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Comparative evaluation of nutritional values in different forage sources using in vitro and in vivo rumen fermentation in Hanwoo cattle

  • Lee, Hu Seong;Lee, Sung Dae;Lee, Seul;Sun, Sangsoo;Kim, Minseok;Choi, Hyuck;Lee, Yookyung;Baek, Youl-Chang
    • Korean Journal of Agricultural Science
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    • v.47 no.4
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    • pp.941-949
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    • 2020
  • This study evaluated the nutritional value of Italian ryegrass (IRG) as a forage source for Hanwoo. The nutritional value of IRG was assessed and compared to that of rice straw, oat hay, and timothy hay using two different methods: 1) in vitro ruminal fermentation 2) in vivo total tract digestibility. In vitro DM digestibility was lower in rice straw compared to the other three forage sources after both 24 and 48 h of incubation (p < 0.01). Among the four forage sources, IRG had a higher NH3-N concentration after both 24 and 48 h of incubation (p < 0.01). In the in vivo digestibility trials, four different substrates were used: 1) 80% concentrate with 20% rice straw, 2) 80% concentrate with 20% oat hay, 3) 80% concentrate with 20% IRG, and 4) 80% concentrate with 20% timothy hay. The dry matter, crude protein, non-fiber carbohydrate, and detergent fiber digestibility were the greatest in the C80-IRG20 among the four forage groups. In summary, IRG had a similar level of energy efficiency compared to oat hay and timothy hay. Furthermore, the result of the chemical composition analysis showing a higher ammonia concentration in the in vitro fermentation experiment and the high protein digestibility in the in vivo experiment indicate that IRG is a good source of protein compared to oat hay and timothy hay.

Using Taguchi design of experiments for the optimization of electrospun thermoplastic polyurethane scaffolds

  • Nezadi, Maryam;Keshvari, Hamid;Yousefzadeh, Maryam
    • Advances in nano research
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    • v.10 no.1
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    • pp.59-69
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    • 2021
  • Electrospinning is a cost-effective and versatile method for producing submicron fibers. Although this method is relatively simple, at the theoretical level the interactions between process parameters and their influence on the fiber morphology are not yet fully understood. In this paper, the aim was finding optimal electrospinning parameters in order to obtain the smallest fiber diameter by using Taguchi's methodology. The nanofibers produced by electrospinning a solution of Thermoplastic Polyurethane (TPU) in Dimethylformamide (DMF). Polymer concentration and process parameters were considered as the effective factors. Taguchi's L9 orthogonal design (4 parameters, 3 levels) was applied to the experiential design. Optimal electrospinning conditions were determined using the signal-to-noise (S/N) ratio with Minitab 17 software. The morphology of the nanofibers was studied by a Scanning Electron Microscope (SEM). Thereafter, a tensile tester machine was used to assess mechanical properties of nanofibrous scaffolds. The analysis of DoE experiments showed that TPU concentration was the most significant parameter. An optimum combination to reach smallest diameters was yielded at 12 wt% polymer concentration, 16 kV of the supply voltage, 0.1 ml/h feed rate and 15 cm tip-to-distance. An empirical model was extracted and verified using confirmation test. The average diameter of nanofibers at the optimum conditions was in the range of 242.10 to 257.92 nm at a confidence level 95% which was in close agreement with the predicted value by the Taguchi technique. Also, the mechanical properties increased with decreasing fibers diameter. This study demonstrated Taguchi method was successfully applied to the optimization of electrospinning conditions for TPU nanofibers and the presented scaffold can mimic the structure of Extracellular Matrix (ECM).

Study on the Electromagnetic Shielding of Accessory Device without Electromagnetic Shielding Technology in the Magnetic Resonance Room (자기공명검사실 내 전자기파 차단이 이루어지지 않은 부속장치의 차폐에 관한 연구)

  • Son, Soon-Yong
    • The Journal of the Korea Contents Association
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    • v.21 no.2
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    • pp.431-436
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    • 2021
  • In this study, a new shielding method was applied to an accessory device that produces electromagnetic waves in the magnetic resonance room to prevent the generation of artifact caused by electromagnetic waves. The research method applied a new shielding made of metal plating fiber to patient surveillance CCTVs without shielding technology, and obtained and evaluated noise map when the power was not cut off and when the new shielding technology was applied without shutting down the CCTV. As a result of the study, it was found that there was at least one group with significant differences. Type I and type III belonged to group 1 while type II belonged to group 2 in the Post-hoc analysis, which meant blocking power of the CCTV and the applying new shielding technology were in the same group. In conclusion, if electromagnetic waves are generated due to additional accessories in the scanning room, the shielding material proposed in this study should be applied which enables the electric state become similar to type I, not generating noise, thereby preventing the artifacts caused by electromagnetic waves.

The Association between Serum Uric Acid Levels and the Nutrients Adequacy Ratio among Male Adult Koreans -Based on the 2016~2019 Korea National Health and Nutrition Examination Survey- (성인남자의 요산농도에 따른 혈액 지표 및 영양소 적정섭취비와 관련성 -국민건강영양조사(2016~2019년)자료를 이용하여-)

  • Chae, Su-Jin;Lee, Geum-Seon;Kim, Sun-Hee;Ryu, Hye-Sook;Yun, Mi-Eun
    • Journal of the Korean Dietetic Association
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    • v.28 no.3
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    • pp.169-181
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    • 2022
  • This study evaluated the correlation between serum uric acid level and the nutrients adequacy ratio (NAR) using data from the Korea National Health and Nutrition Examination Survey (KNHANES) 2016~2019. This is a cross-sectional study in which 6,579 Korean male adults were divided into quartiles according to their uric acid levels. All analyses were performed with adjusted age. Uric acid decreased as the age increased from the age group 19~29 years to 30~49 years and then to 50~64 years. Interquartile results of serum uric acid levels showed that increases in the serum uric acid levels were associated with decreases in LDL-cholesterol (P<0.01), triglycerides (P<0.001), body weight (P<0.001), waist circumference (P<0.001), body mass index (P<0.001), systolic blood pressure (SBP) (P<0.001), diastolic blood pressure (DBP) (P<0.001). However, uric acid levels decreased with an increase in HDL cholesterol (P<0.001). The analysis of the NARs according to the quartiles of serum uric acid levels in men showed that an increase in serum uric acid levels was associated with a decrease in the NAR levels of dietary fiber (P<0.001), calcium (P<0.001), and folic acid (P<0.001).

Anatomical Characteristics of Major Korean Ash Species (한국산 물푸레나무속(屬) 주요 수종의 해부학적 특성)

  • Hwang, Won-Joong;Kwon, Goo-Joong;Park, Wan-Geun;Bae, Young-Soo;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.2
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    • pp.79-86
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    • 2002
  • Anatomical characteristics of Mulpurenamu (Korean ash, Praxmus rhynchopbylla), Deulmaenamu (Manshurican ash, Fraxinus mandsburica) and Sheamulpure (Sieboldiana ash, Fraxinus sieboldiana) grown in Korea were examined by an optical microscopy and an image analysis. Some characteristics such as boundary of annual rings, shape and size of vessel elements, arrangement of axial parenchyma cells in cross section, and cell volumetric composition showed significant differences between the sample species. In radial variation of elements, fiber length and vessel size increased from the pith for about 10 to 15 years and then reached a more or less constant. The results of this study can be used for identification of wood and indices of wood quality in Fraxinus spp.

Net energy and its establishment of prediction equations for wheat bran in growing pigs

  • Zhiqian, Lyu;Yifan, Chen;Fenglai, Wang;Ling, Liu;Shuai, Zhang;Changhua, Lai
    • Animal Bioscience
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    • v.36 no.1
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    • pp.108-118
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    • 2023
  • Objective: The objective of this experiment was to determine the net energy (NE) value of 6 wheat bran and 1 wheat shorts by indirect calorimetry and establish the NE prediction equations of wheat bran fed to growing barrows. Methods: Forty-eight growing barrows (28.5±2.4 kg body weight) were allotted in a completely randomized design to 8 dietary treatments that included a corn-soybean meal basal diet, 6 wheat bran diets and 1 wheat shorts diet. The inclusion level of wheat bran or wheat shorts in diets is 30%. Results: The addition of wheat bran reduced the apparent total tract digestibility (ATTD) of nutrients (p<0.05). The ATTD of gross energy, crude protein (CP) and dry matter (DM) in the wheat shorts were greater than that in the wheat bran. Addition of wheat bran or wheat shorts had no effect on total heat production and fasting heat production. The NE of wheat bran was negatively correlated with neutral detergent fiber (r = -0.84; p<0.05) and acid detergent fiber (r = -0.83; p<0.05), while it was positively correlated with CP (r = 0.92; p<0.01). The NE values of wheat bran ranged from 6.79 to 8.15 MJ/kg DM, and the NE value of wheat shorts was 12.47 MJ/kg DM. The ratio of NE to metabolizable energy for wheat bran fed to growing pigs was from 66.0% to 71.7%, whereas the value for wheat shorts was 83.7%. Conclusion: The NE values of wheat bran ranged from 6.79 to 8.15 MJ/kg DM, and the NE value of wheat shorts was 12.47 MJ/kg DM. The NE value of wheat bran can be well predicted based on energy content and proximate analysis.

The non-saponin fraction of Korean Red Ginseng ameliorates sarcopenia by regulating immune homeostasis in 22-26-month-old C57BL/6J mice

  • Oh, Hyun-Ji;Jin, Heegu;Lee, Boo-Yong
    • Journal of Ginseng Research
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    • v.46 no.6
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    • pp.809-818
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    • 2022
  • Background: The non-saponin fraction (NSF) of Korean Red Ginseng is a powder in which saponin is eliminated from red ginseng concentrate by fractionation. In this study, we examined the effect of NSF on age-associated sarcopenia in old mice. Methods: NSF (50 or 200 mg/kg/day) was administered orally daily to young (3-6-month-old) and old (20-24-month-old) C57BL/6 J mice for 6 weeks. Body weight and grip strength were assessed once a week during the oral administration period. The gastrocnemius and quadriceps muscle were excised, and the muscle fiber size was compared through hematoxylin and eosin staining. In addition, the effect of NSF on sarcopenia and inflammation/oxidative stress-related factors in hindlimb muscles was investigated by western blotting. Flow cytometry analysis was conducted to investigate the effect of NSF on immune homeostasis. Blood samples were collected by cardiac puncture, and the serum levels of insulin-like growth factor 1, pro-inflammatory cytokines, and glutathione were evaluated. Results: NSF significantly alleviated muscle strength, mass, and also fiber size in old mice. Age-associated impairment of immune homeostasis was recovered by NSF through retaining CD11b+F4/80+ macrophages and regulating inflammatory biomarkers. NSF also decreased the age-induced expression of oxidative stress factors. Taken together, NSF showed the effect of improving sarcopenia by inhibiting low-grade chronic inflammatory/oxidative stress factors. Conclusion: NSF exhibited anti-sarcopenia effects by regulating chronic inflammation and oxidative stress in old mice. Thus, we suggest that NSF is a promising restorative agent that can be used to improve sarcopenia in the elderly as well as maintain immune homeostasis.

Effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass (Pennisetum purpureum Schum.) silage

  • Zhao, Guoqiang;Wu, Hao;Li, Li;He, Jiajun;Hu, Zhichao;Yang, Xinjian;Xie, Xiangxue
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1301-1313
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    • 2021
  • This study investigated the effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass silage after ensiling for 30 d. Three groups were studied: No additives (control); added cellulase (Group 1); and added cellulase and starch (Group 2). The results showed that the addition of cellulase and starch decreased the crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and pH significantly (p < 0.05) and increased water-soluble carbohydrate (WSC) content (p < 0.05). The addition of additives in two treated groups exerted a positive effect on the lactic acid (LA) content, lactic acid bacteria (LAB) population, and lactic acid / acetic acid (LA/AA) ratio, even the changes were not significant (p > 0.05). Calculation of Flieg's scores indicated that cellulase application increased silage quality to some extent, while the application of cellulase and starch together significantly improved fermentation (p < 0.05). Compared with the control, both additive groups showed increased microbial diversity after ensiling with an abundance of favorable bacteria including Firmicutes and Weissella, and the bacteria including Proteobacteria, Bacteroidetes, Acinetobacter increased as well. For alpha diversity analysis, the combined application of cellulase and starch in Group 2 gave significant increases in all indices (p < 0.05). The study demonstrated that the application of cellulase and starch can increase the quality of Napier grass preserved as silage.

The Ingestion of Dietary Prebiotic Alternatives during Lactation Promotes Intestinal Health by Modulation of Gut Microbiota

  • Sangdon Ryu;Jeong Jae Lee;Daye Mun;Soo Rin Kim;Jeehwan Choe;Minho Song;Younghoon Kim
    • Journal of Microbiology and Biotechnology
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    • v.32 no.11
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    • pp.1454-1461
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    • 2022
  • Palm kernel expeller (PKE), a by-product of palm oil extraction, contains higher amounts of fiber than corn and soybean meal, but offers low energy density, protein value, and amino acid (AA) composition, limiting its use for swine. Recently however, it was reported that dietary fiber has a positive effect on the gut microbiota of the host, and therefore it is necessary to study the effect of PKE feeding on the intestinal microbiota of swine. In this study, we investigated the effects of supplementation with PKE in lactation diets on the gut microbiota composition of lactating sows and their litters. A total of 12 sows were randomly assigned to two dietary treatment groups in a completely randomized design. The treatments were a diet based on corn-soybean meal (CON) and CON supplemented with 20% of PKE. Sow and piglet fecal samples were collected before farrowing, on days 7 and 28 (weaning) after farrowing, and on days 7 and 28 (weaning) after farrowing, respectively, to verify gut microbiota composition by pyrosequencing analysis. The beta-diversity result showed a significant difference only in weaning-stage piglets, but dietary PKE altered the gut microbiota in sows by increasing the abundance of Lactobacillus compared with CON. In piglets, dietary PKE decreased the abundance of opportunistic pathogen Proteus and increased the abundance of potentially beneficial bacteria, such as Prevotellaceae and Prevotella. Our results can be helpful in developing feeding strategies and support the beneficial effects of dietary PKE to improve the gut health of animals.