• Clinical and Experimental Reproductive Medicine
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• 제22권2호
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• pp.211-220
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• 1995

Though the endometriosis is not always related with infertility, endometriosis causes infertility in some patients. There are many treatment modalities of infertile patients who have endometriosis. In recent years, Assisted Reproductive Technology(ART) have been widely accepted as being a useful tool for the treatment of infertile endometriotic patients. The objective of this study was to evaluate the outcome of ART in infertile endometriotic patients who have been carried out IVF-ET from Jan, 1992 to Dec, 1994 and to compare the results between COH/IUI and IVF-ET in the patients with endometriosis stage I. Tubal disease only patients were grouped(308 patient, 956 cycles) as a control. Endometriosis group was subdivided into 4 groups according to American Fertility Society classification; endometriosis stage I (45 patients, 61 cycles), stage II (26 patients, 39 cycles), stage III (26 pateitns, 37 cycles), stage IV (33 patients, 50 cycles). The outcomes of IVF-ET in endometriosis patients were as follows; The oocyte recovery rates were significantly lower in stage III, IV endometriosis. In case of stage III endometriosis, the fertilization rate was significantly lower than other stages of endometriosis. Clinical pregnancy rates per cycle were not different between the tubal group(22%) and the endometriosis group(25%). According to endometriosis stage, the implantation rate and clinical pregnancy rate were significantly lower in stage IV (5.6%, 16%) compared with other stages (I; 10.0%, 26%, II;9.8%, 31%, III;12.6%, 32%). It suggests that some factor like autoantibodies may inhibit implantation of embryos in stage IV endometriosis. To evaluate the possibility that simply increasing the number of gametes at the site of fertilization might account for pregnancies attributed to IVF-ET, the authors retrospectively analyzed the outcome of couples undergoing IUI during hMG cycles and CC cycles between 1992 and 1994 in the women with endometriosis stage 1. In case of stage I endometriosis, though the COH/IUI group showed lower FSH level and lesser age profile than IVF-ET group, IUI group has resulted in lower pregnancy rates(19.2%) compared with the IVF-ET group(26.2%). In conclusion, endometriotic infertile patients can get comparable pregnancy rates with the tubal factor infertility patients during IVF-ET program. Moreover even in stage I endometriosis, IVF-ET may be an more effective treatment modality than COH/IUI.

• 한국토양비료학회지
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• 제42권5호
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• pp.371-378
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• 2009

본 시험은 벼 재배시 녹비작물로 녹비용 보리의 투입효과를 구명하여 호밀 대체 가능성을 검토코자 수행하였다. 호밀은 출수기, 녹비용 보리는 출수기, 출수 후 3일 및 출수 후 10일에 경운로타리 작업을 하고 담수시켜 부숙을 촉진한 다음 녹비작물 무시용구는 표준시비, 녹비작물 시용구는 토양검정시비 및 무시비 하여 동진 1호를 재배 후 토양물리화학성, 녹비작물 부숙정도, 생육 및 수량성을 검토한 결과는 다음과 같다. 녹비작물 투입시기별 생체중은 각각 10a당 2,715, 2,352, 2,867 kg이였고 건물중에 있어서 총질소 함량은 1.31, 1.46, 1.38%이었으며 탄질률은 33.4, 28.7, 34.6이였다. 녹비작물 투입으로 작토심이 깊어졌고 토양경도와 용적밀도가 낮아졌으며 공극률이 증가하여 물리성이 개선되었다. 또한 시험 전에 비하여 녹비용 보리 환원으로 양이온치환용량이 높아졌으며 치환성 양이온 함량이 증가되는 경향을 나타냈다. 쌀 수량은 관행($559kg\;10a^{-1}$)에 비하여 녹비용 보리+토양검정진단시비구에서 3~9% 증수되었으며, 현미 외관품위 중 완전립비율은 관행(73.0%)에 비하여 녹비용 보리 투입시 73.6~78.7%로 높은 경향을 나타냈다. 결론적으로 녹비용 보리를 출수 후 10일경 투입하여 벼 재배시 질소비료 절감과 지력개선에 효과적인 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1566-1573
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• 2006

The study was conducted on 30 true acyclic Sahiwal cows (15 cows, ${\geq}90$ days postpartum; 15 postpubertal heifers, ${\geq}30$ months of age) and a similar 20 untreated controls (10 cows, 10 heifers). An 'Eazi' breed Controlled Internal Drug Release (CIDR) device (containing 1.38 g progesterone) was inserted intravaginally for 7 days (days 0 to 7) followed by 500 IU eCG i.m. at CIDR removal in all the treated animals. Heifers also received 5 mg oestradiol valerate i.m at CIDR insertion. The reproductive performance of these animals was recorded in terms of oestrus induction response, conception and pregnancy rates. Plasma progesterone ($P_4$) and oestradiol-$17{\beta}$ ($E_2$) profiles of 4 representative animals from each treatment group before, during and after CIDR treatment were also monitored. An oestrus induction response of 100% was observed in treated cows and heifers. The majority of cows (53.3%) and heifers (60%) were induced to oestrus within 24-36 and 36-48 h, respectively after CIDR withdrawal; with mean intervals of $44{\pm}3.18$ and $48{\pm}2.35h$, respectively. The conception rate at induced oestrus was higher in cows (40%) than heifers (20%). The final pregnancy rates after 2 subsequent oestruses were 80 and 60% in cows and heifers, respectively (overall 70% for all treated animals). In comparison, only 10% of control animals (2 cows only, 2/20) showed oestrus and become pregnant (10%) during theentire study period. The pretreatment (day 0) mean plasma P4 levels were statistically (p>0.05) similar in cows and heifers ($0.40{\pm}0.04$ and $0.49{\pm}0.11ng/ml$, respectively). The peak $P_4$ levels were observed on day 1 in cows ($13.94{\pm}1.41ng/ml$) and day 2 in heifers ($19.15{\pm}3.30ng/ml$) with a progressive decline up to the day of CIDR withdrawal ($3.35{\pm}0.92$ and $8.79{\pm}1.71ng/ml$, respectively). Mean $P_4$ levels on day 9 and 10 in cows and heifers did not differ significantly from their respective day 0 values and the lowest values were recorded on day 10 both in cows and heifers ($0.13{\pm}0.03$ and $0.14{\pm}0.02ng/ml$, respectively). Wide variations in individual pretreatment $E_2$ levels were observed both in the cows (range = 4-26, mean = $13.00{\pm}4.65pg/ml$) and heifers (range = 10-14, mean = $11.50{\pm}0.96pg/ml$). Thereafter also, $E_2$ levels in cows showed variation and reached a peak level ($53.50{\pm}2.99pg/ml$) on day 8. In heifers, peak mean $E_2$ level ($111.25{\pm}39.81pg/ml$) was recorded on day 1, followed by a non-significant decline on day 2, a significant fall on day 6 and a non-significant increase on day 9 and 10. However, mean $E_2$ levels on days 7 (p<0.05), 8 and 9 (p<0.01) were significantly higher in cows compared to heifers. The post-CIDR withdrawal mean highest $P_4$ and lowest $E_2$ levels coincided with the period when the majority of animals were induced to oestrus. CIDR and eCG treatment resulted in effective induction of oestrus with satisfactory pregnancy rates in true acyclic Sahiwal cows and heifers.

• 한국가축번식학회지
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• 제14권1호
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• pp.36-42
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• 1990

The effect of preincubation on in vitro maturation and fertility were investigated using preovulatory oocytes with and without cumulus cells obtained from superovulated ouot-bred ICR mice. Oocytes were recovered from fully grown folicle at 10 hr after hCG administration. A part of oocytes recovered were treated with the solution of 0.1% hyaluronidase to remove cumulus cells. Both intact and treated oocytes were then incubated for 0 to 6hr in mT6 medium containing 0.3% BSA. After incubation for various times, a part of oocytes were subjected to the investigation of nuclear maturation and the remaining oocytes were used fro the induction of in vitro fertilization by adding them into medium containing capacitated mice epididymal spermatozoa. Above all, the percentage of preovulatory oocytes at the stage of metaphase II after preincubation for 0, 2, 4 and 6hr was 15.8, 36.4, 47.5 and 66.7%, respectively, suggesting the in vitro maturation of oocytes during their incubation. On the other hand, fertilizatin rate of oocytes preincubated for 0, 2, 4 and 6hr with and without cumulus cells were 41.0, 58.7, 68.7 and 75.6%, and 50.0, 45.1, 37.8 and 39.2%, respectively. No significant differences in fertilization rate between preovulatory oocytes preincubated for 6hr with cumulus cells and naturally ovulated were observed. These results suggest that cumulus cells take very important role in maturtion of oocytes in vitro. The precentage of preovulatory oocytes developed to 2-cell stage in vitro fertilization following preincubation for 0 to 6hr with and without cumulus cells ranged from 48.5 to 82.4% and 36.9 to 56.1%, respectively. Also, the rates of oocytes developed to blastocyst in vitro fertilization after preincubation for 0 to 6hr with and without cumulus cells were 28.1, 39.3, 42.5 and 44.0% and 12.5, 32.6, 24.4 and 15.5%, respectively. From these results, it could be said that fertility of preovulatory oocytes with cumulus cells could be improved to the level of that of naturally ovulated oocytes by adquate preincubation in vitro. Cumulus cells may, therefore, affect in vitro maturation, fertilization and following development of oocytes by influencing zona hardening.

• 대한간호학회지
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• 제3권3호
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• pp.50-61
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• 1973

Despite the intensive family Planning program of the government, which has reduced the :average population growth to 2.0 percent in 1970, the continuing high fertility rates and poor family planning services in urban slum areas has been continually pointed out by various evaluation seminars. Thus, it was felt that a study of the current status of family planning in an urban slum population was needed. The aims of the study were to: (1) delineate the general characteristics of the urban slum population (2) discover the knowledge, attitudes, and practice of family planing. (3) determine what was felt to be the ideal number of children. (4) determine the status of induced abortion. A random stratified sample of 200 women was selected from a population of 3, 118 married women, 20-49 years of age, living in the Yonsei Community Health Project area and registered at the Yonhee Community Health Service Institute. The respondents were interviewed by the investigates, using a pretested questionary, from the period of October 1 to October 30, 1972. The findings of the Study were as follows: 1. Afore than half of the respondents were in the high fertility age group (25-34): of lower educational level (under primary School) : from rural areas: and with unstable sources of income. 2. The average respondent had 3.6 living children. 3. Most of the respondents had knowledge of contraceptive methods such as the Loop(78, 5%) and Oral pill (87.0%). 4. Seventy seven percent of the respondents recognized the Health Center as an available source for family planning. 5. About seventy percent of the respondents approved of family planning. 6. Eighty four point five percent of all the respondents answered that using contraceptives is harmful to maternal health. 7. Currently, 21.0 percent of the respondents were using contraceptives, while 40.0 percent of the respondents were current users or had used contraceptives. 5. Of the respondents who had ever stopped using contraceptives, 78.0 percent gave side effects as the reason they discontinued the method. 9. The average number of ideal children reported by the respondents was 3.5. 10. If they had only 2 daughters, 85.5 Percent of the respondents thought they must have more children. 11. Sixty nine point five percent of the respondents approved of induced abortion for unwanted pregnancies, while 34.5 percent of the respondents had experienced induced abortion for unwanted pregnancies. The mean number of induced abortion for unwanted pregnancies was 0.7. 12. The result of this study showed that such urban slum population would be one of the most important target of the family planning services, therefore further social-psychological researches with technical development on this area are recommended.

• 한국토양비료학회지
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• 제47권6호
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• pp.586-593
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• 2014

Newly reclaimed tidal land is known to be in low status of soil fertility. The incorporation of crop residue is an effective method to improve soil properties and fertility in reclaimed saline soils. The objective of this study was to evaluate the efficiency of rice straw (RS) application to improve physico-chemical properties of saline-sodic soil and its contribution to productivity of whole crop barley. Increasing rate of rice straw improved growth parameter related to yield of whole crop barley, which increased tiller number significantly (p<0.05).The yield increased by 15% (F.W) and 9% (D.W) in rice straw-amended plots. The content of soil organic matter (SOM) in the surface soil (0-20cm) with rice straw incorporation increased by 5~9% (RS 2.5~RS 7.5) compared to RS 0, in which the content of SOM decreased after two consecutive cultivations. Rice straw incorporation promoted soil physico-chemical properties and nutrient-availability of the test crop, as indicated in change in soil bulk density, porosity and increased nutrient uptake of plant. Especially, the P content and uptake of whole crop barley increased with increasing the rate of rice straw application. In conclusion, the rice straw application at rates of $5.0-7.5ton{\cdot}ha^{-1}$ in reclaimed saline soils effectively improved soil properties and crop productivity, which has potentials to reduce the loss of chemical fertilizers and facilitate the favorable condition for crop growth under adverse soil condition.

• Clinical and Experimental Reproductive Medicine
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• 제38권4호
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• pp.193-202
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• 2011

Objective: We found previously that $interferon$ $regulatory$ factor ($Irf$)-1 is a germinal vesicle (GV)-selective gene that highly expressed in GV as compared to metaphase II oocytes. To our knowledge, the function of $Irf-1$ in oocytes has yet to be examined. The present study was conducted to determine the relationship between retinoic acid (RA) and RA-mediated expression of $Irf-1$ and the mouse oocyte maturation. Methods: Immature cumulus-oocyte-complexes (COCs) were collected from 17-day-old female mice and cultured $in$ $vitro$ for 16 hours in the presence of varying concentrations of RA (0-10 ${\mu}M$). Rate of oocyte maturation and activation was measured. Gene expression was measured by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and cytokine secretion in the medium was measured by Bio-Plex analysis. Apoptosis was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Results: The rates of oocyte maturation to metaphase II and oocyte activation increased significantly with RA treatment (10 nM-1 ${\mu}M$). With 100 nM RA treatment, lowest level of $Irf-1$ mRNA and cumulus cell's apoptosis was found. Among 23 cytokines measured by Bio-Plex system, the substantial changes in secretion of tumor necrosis factor-${\alpha}$, macrophage inflammatory protein-$1{\beta}$, eotaxin and interleukin-12 (p40) from COCs in response to RA were detected. Conclusion: We concluded that the maturation of oocytes and $Irf-1$ expression are negatively correlated, and RA enhances the developmental competence of mouse immature oocytes $in$ $vitro$ by suppressing apoptosis of cumulus cells. Using a mouse model, results of the present study provide insights into improved culture conditions for $in$ $vitro$ oocyte maturation and relevant cytokine production and secretion in assisted reproductive technology.

• 한국토양비료학회지
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• 제18권3호
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• pp.297-303
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• 1985

땅콩에 대(對)한 삼요소(三要素)의 시비효과(施肥效果)와 땅콩 재배지(栽培地) 농가포장(農家圃場)의 토양비옥도상태(土壤肥沃度狀態)를 파악(把握)하여 토양개량(土壤改良)과 합리적(合理的) 시비(施肥)를 위(爲)한 기초자료(基礎資料)를 얻고자 삼개년간(三個年間) 시험(試驗)을 수행(遂行)하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 땅콩에 대(對)한 삼요소(三要素) 적정시비량(適正施肥量)은 $N-P_2O_5-K_2O$ 각각(各各) 8.6-10.9-10.4kg/10a 이었다. 2. 질소(窒素), 인산(燐酸), 가리(加里)의 각(各) 결제구(缺除區)에 대(對)한 시비효과(施肥效果)는 각각(各各) 23, 8, 16%, 질소(窒素), 인산(燐酸), 가리(加里)의 시비효율(施肥效率)은(비료각성분(肥料各成分) kg당(當) 증수량(增收量))은 각각(各各) 6.0, 1.8, 3.4kg이었다. 3. 땅콩 재배(栽培)를 위(爲)한 토양중(土壤中) 적정(適正) 유효인산(有效燐酸) 및 치환성가리함량(置換性加里含量)은 각각(各各) 250ppm, 0.8me/100g로 추정(推定)되었다. 4. 땅콩 재배농가포장(栽培農家圃場)의 토양옥비도(土壤沃肥度) 조사결과(調査結果) 토양중(土壤中) 유효성분함량(有效成分含量)의 대부분이 적정함량(適正含量) 범위(範圍)에 크게 미달(未達)되었다.

• 한국환경농학회지
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• 제31권4호
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• pp.308-312
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• 2012

본 연구는 녹비작물-벼 재배 작부체계에서 볏짚과 녹비작물을 전량 회수하여 사료작물로 활용시 농경지 비옥도 관리를 위한 양분수지를 평가하였다. 녹비보리와 헤어리베치 혼파비율에 따른 녹비작물 생산량은 B75H25에서 가장 높게 나타났다. B75H25 처리구의 벼 수량은 NPK 처리구 대비 90%를 보였다. 녹비작물과 볏짚을 전량 회수시 NPK, B100, B75H25, B50H50, B25H75, H100 처리구의 질소 수지는 각각 2.0, -80.8, -104.0, -93.5, -82.9, 76.2 kg N/ha 이었다. 그리고 질소 수지가 가장 낮은 B75H25 처리구의 인산수지는 -50.3 kg P/ha로 가장 낮았다. 따라서 녹비작물-벼 재배 작부체계에서 녹비작물과 볏짚을 전량 사료로 활용시 농경지 비옥도 유지를 위해서 각각 약 90 kg N /ha, 50 kg P/ha의 질소와 인산 시비가 필요한 것으로 나타났다. 그리고 금후 녹비작물을 사료로 활용하고자 할 경우 화학비료 시용량과 적정 녹비작물의 회수량에 관한 연구가 필요한 것으로 판단된다.

• 한국육종학회지
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• 제40권3호
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• pp.263-268
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• 2008

잣나무 채종원의 유전간벌을 위하여 재적생장에 대한 개량 효과와 유효클론수를 이용한 유전다양성을 평가하였다. 유전 간벌은 클론의 일반조합능력으로 나타나는 육종가(breeding value), 암꽃과 수꽃 개화량을 이용한 종자생산 기여도, 클론별 ramet수 등를 이용한 클론별 변이를 기초로 하였다. 차대 검정 결과에 의하여 산출된 재적생장에 대한 클론별 일반조합능력(genetic value) 및 1991년부터 2003년까지 조사된 개화량 성적을 이용하여 잣나무 채종원(1981년 조성) 179클론에 대하여 간벌 강도에 따른 유전다양성 및 개량효과를 분석하였다. 이때 채종원 외부로부터의 화분오염율은 30%(gene migration = 15%)로 설정하였다. 그 결과 재적생장에 대한 개량효과는 간벌율이 10%에서 60%까지 높아짐에 따라서 점차 증가하였다. 하지만 이보다 더 높은 간벌 강도에서 현저한 개량효과 증가는 나타나지 않았다. 유효클론수는 간벌강도 10%에서 60% 사이에서는 40클론으로 나타났으며, 70% 간벌율 이상에서는 증가하거나 감소하는 변동을 나타냈다. 따라서 본 잣나무 채종원에 대해서는 50%에서 60% 수준의 간벌 강도가 적당한 것으로 판단된다.