• Journal of the Korean Society of Food Science and Nutrition
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• v.1 no.1
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• pp.51-62
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• 1972

In Korea, fermented fish has been playing an important role as a preserved and flavor rich food. It is said that the digestion of fish protein is due to both action of intrinsic (autolytic enzymes) and bacterial enzymes in fish. The mass production of fermented fish has been impeded since traditional method of fermentation requires a long duration for a complete digestion. A high concentration of salt and unsanitary condition are also considered disadvantages of the old method. To improve the quality of the product and to develop mechanized process of fermentation, fermentors which have such control device as temperature, pH and agitation control system have been urgently needed. In this study, a model design of a fermentor is studied. The calculation was based on the optimum conditions for enzymatic hydrolysis of fish protein which involve temperature, pH, viscosity and other factors.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.247-253
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• 2005

The bacterial cellulose (BC) production by a wild­strain Acetobacter xylinum BPR2001 and that by its acetan­nonproducing mutant, EPI, were compared in a jar fermentor. EPI produced about $28\%$ less BC than the wild-strain. The apparent difference in the cultivation of the two strains was the viscosity increase in the culture broth that was closely associated with acetan production. Increasing the viscosity of the culture broth of EPI by adding agar led to the formation of relatively small and uniform BC pellets, and BC production consequently became two-fold higher than that in the absence of agar and was almost equal to that by BPR2001. Therefore, acetan has an important role in BC production by inducing physical changes in the culture broth of the wild-type strain.

• The Korean Journal of Food And Nutrition
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• v.1 no.2
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• pp.43-49
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• 1988

Fermented barley food was produced by the combining action of an enzyme and a lactobacillus. When Lactobacillus sp. L-5 and commercial liquefying amylase from Tae Pyeong Yang Chemical Co. were selected, inoculated on steamed barley and cultivated at 37$^{\circ}C$ for 48hrs, the fermented product of good quality was obtained. In batch cultivation using rotary drum fermentor, viable cell count reached 1.1$\times$10CFU/g after 12hrs' cultivation, and specific growth rate in logarithmic phase was 0.6hr-1. Viable cell count, acidity, pH, concentration of reducing sugar and viscosity of the 48hrs' fermentation product from rotary drum fermentor was 4.3$\times$108CFU/g, 1.17%, 3.1, 10.7% and 1430cp.

• KSBB Journal
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• v.13 no.6
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• pp.675-680
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• 1998

In both 7L and 20L fermentor experiments the level of dissolved oxygen (D.O) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. increased carbon substrate consumption rate and cell growth rate with a similar residual biomass production. However, a lower D.O. was a much better condition for PHBV production. In a 20L fermentor experiments controlled at 5% D.O. cell growth rate was about twice faster(0.555 hr-1 and 0.260 hr-1 at the acid and the glucose phase, respectively) with an equal amount(4.5 g/L) of residual biomass production. However, PHBV content in the cell(62.3 wt%) increased 17.3 times at 1% D.O.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.5
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• pp.303-305
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• 2003

Scale-up of hirudin production from Saccharomyces cerevisiae from bench-scale to pilot-scale was carried out based on constant volumetric oxygen transfer coefficient (K$\sub$L/a). Fed-batch mode of cultivation using step-wise feeding strategy of galactose was employed for the production of hirudin in a 30-L and a 300-L pilot-scale fermentor. The final hirudin concentrations were achieved 390 mg/L and 286.1 mg/L, and the volumetric productivities were 80.4% and 90.7% with the 30-L and 300-L fermentors, respectively, compared to the productivity of the 5-L bench-scale fermentor.

• KSBB Journal
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• v.5 no.4
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• pp.403-410
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• 1990

Application of the Rheocatch Hotwire Monitoring System for food biotechnology process was evaluated. The growth of microogranism, E coli (JM 83 and Sigma) and Corynesccfertun glutamicum, were monitored. in the fermentor. The cell growth could not be detected the temperature differences between the hotwire and samples($\Delta$T) as indicated by the monitoring system during the fermentation processes. The cell concentration of less than 2g/dl was not sufficient to generate the measurable temperature difference in the fermentor. In order to calibrate the Rheocatch Monitoring System, the temperature difference as a function of solute concentration (microbial cells, sodium cholide, sucrose and dextran) was studied. The relationship between $\Delta$T and the concentration of microbial cells, sucrose and dextran can be expressed in a power series. Further studied with dextran indicated that viscosity and/or kinematic viscosity increase exponentially with an increase in $\Delta$T This is regardless of the concentration and molecular weight of dextran. $\Delta$T linearly increases with the logarithm of molecular weight, while the logarithm of viscosity and the logarithm of kinematic viscosity increase with the logarithm of molecular weight.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.292-295
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• 2002

A chromate-resistant white rot fungus, Inonotus cuticularis, abundant in oak trees, was isolated for chromate removal and detoxification of chromate. Inonotus cuticularis was also investigated for an optimal waste treatment system. The screened cells were able to reduce an initial chromate concentration of as high as 1,300 ppm. Cell growth kinetics showed that the optimum culture conditions in flasks were at $33^{\circ}C$ and pH 4.2. Furthermore, the cells were able to remove $54\%$ of the initial chromate by a two-stage operation based on the combination of a fermentor and airlift reactor.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.628-631
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• 2000

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.6
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• pp.362-366
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• 2002

A mathematical model has been developed for describing the oxygen concentration during the exponential growth of microorganisms, in a static solid substrate bed supported on a tray fermentor. The model equations comprise of one partial differential equation for mass transfer and an ordinary differential equation of growth. After nondimensionlisation, analytical solution tn the model has been obtained by the method of Laplace transforms. An expression for critical thickness of bed is deduced from the model equation. The significance of the model in the design of tray fermentors is discussed. The validity of the discussion is verified by taking an illustration from the literature.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.42-42
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• 2000