• Korean Journal of Poultry Science
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• v.27 no.2
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• pp.133-153
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• 2000

The egg's interior quality is one of the most important criteria for commercial producers and consumers. Internal quality is complex, including aesthetic factors such as taste, freshness, nutritional and processing values, and the genetic influences upon these upon these factors ranges from none to considerable. The rate of cholesterol synthesis in the hen is very high compared to other animals and humans. Genetic selection, diet drugs and other chemicals can alter cholesterol concentration in the plasma of laying hen, but attempts to manipulate the cholesterol concentration in the egg yolk are generally unsuccessful since the cholesterol can only be changed to a small extent. Factors which may affect the degree of pigmentation of the yolk include the type of xanthophyll and its concentration in the feed, the feed composition, and the health of the hen. Several feed ingredients interact with carotenoid pigment to improve or reduce their deposition rates in yolks. Egg weight is determined by genetics, body size prior to first egg housing density, environmental temperature, lighting program, total feed consumption, calcium, phosphorus, niacin, water, methionine, total sulfur amino acids, energy, linoleic acid, fat and protein levels. Eggs need to be promote levels. Eggs need to be promoted a versatile commodity and new processed egg items need to be developed. Organic eggs are laid by hens which were raised in chemical and drug frdd environments. There are still difficulties in producing these eggs due to the availability of organic poultry feeds and cost of organic grains.

• Journal of Animal Science and Technology
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• v.64 no.5
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• pp.911-921
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• 2022

Maximum residue limits (MRL) for pesticides in feed have been set to protect public health and produce safe livestock products. In vivo experiments to establish MRL are essential, as livestock are commonly used to obtain reliable in vivo quantitative information. Here, we aimed to evaluate whether small laboratory animals can replace or reduce monogastric livestock in experiments to quantify pesticide residues in vivo after oral consumption through feed. First, 24 pigs and rats were randomly assigned to four groups and fed 0, 3, 9, or 30 mg/kg of sulfoxaflor. After four weeks, serum, muscle, fat, liver, kidney, and small intestine samples were collected, and sulfoxaflor residues were analyzed using liquid chromatography - tandem mass spectrometry. Sulfoxaflor residues in pig tissues were significantly correlated with those in rat tissues. Model equations were formulated based on the residual sulfoxaflor amount in pig and rat tissues. The calculated and measured sulfoxaflor residues in pigs and rats showed more than 90% similarity. Sulfoxaflor did not affect body weight gain, feed intake, or the feed conversion ratio. Therefore, we concluded that pesticide residue quantification in vivo to establish MRL could be performed using small laboratory animals instead of livestock animals. This would contribute to obtaining in vivo pesticide residue information and reducing large-scale livestock animal experiments.

• Nuclear Engineering and Technology
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• v.55 no.4
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• pp.1225-1232
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• 2023

The ¹⁸⁰W is the lightest isotope of Tungsten with small abundance ratio. It is slightly radioactive (α decay), with an extremely long half-life. Its separation is possible by non-conventional single withdrawal cascades. The ¹⁸⁰W is used in radioisotopes production and study of metals through gamma-ray spectroscopy. In this paper, single withdrawal cascade model is developed to evaluate multicomponent separation in non-conventional transient cascades, and available experimental results are used for validation. Numerical studies for separation of ¹⁸⁰W in a transient single withdrawal cascade are performed. Parameters affecting the separation and equilibrium time of cascade such as number of stages, cascade arrangements, feed location and flow rate for a fixed number of gas centrifuges (GC) are investigated. The Salp Swarm Algorithm (SSA) as a bio-inspired optimization algorithm is applied as a novel method to minimize the feed consumption to obtain desired concentration in the collection tank. Examining different cascade arrangements, it is observed in arrangements with more stages, the separation is further efficient. Based on the obtained results, with increasing feed flow rate, for fixed product concentration, the cascade equilibrium time decreases. Also, it is shown while the feed location is the farthest stage from the collection tank, the separation and cascade equilibrium time are well-organized. Finally, using SSA optimal parameters of the cascade is calculated, and optimal arrangement to produce 5 gr of ¹⁸⁰W with 90% concentration in the tank, is proposed.

• Fisheries and Aquatic Sciences
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• v.27 no.3
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• pp.195-205
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• 2024

Amphipoda is a benthic fauna occupying aquatic environments that can be used as a live feed for mud crabs. The abundance of amphipods in the water is thought to impact the preying capacity of crablets, which in turn will affect their growth performance. This study aims to determine the preying capacity of the crablet stage of Scylla tranquebarica exposed at different densities (20, 30, and 40 amphipods / 0.5 L) of amphipod, Grandidierella megnae. The preying capacity was estimated by counting the number of amphipods ingested by an individual crablet during the 60-day rearing period. The main parameters measured were daily consumption rate (DCR), Cumulative molting (CM), Feed conversion ratio (FCR), and the specific growth rate of weight (SGR-W). The results showed that the DCR and FCR were not affected by amphipod densities but the higher the amphipod density the higher the SGR-W and CM. Based on this study, 30 amphipods / 0.5 L is recommended as the optimal density to optimize the DCR and improve the growth performance of crablets. It is also proposed that surplus live feed will potentially reduce the cannibalism rate of crablets during weaning. However, more research needs to be carried out to elucidate the benefits of crablet-feeding amphipods in communal systems.

• Korean Journal of Poultry Science
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• v.40 no.1
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• pp.67-73
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• 2013

With an increasing concern on laying hen welfare, barn system has appeared as one of the alternatives to replace the conventional cage. This study was conducted to compare the early laying performance and egg quality at the barn system with those at the conventional cage. A total of 288 shaver-579 brown layers were used for 9 weeks ($21^{st}$ to $30^{th}$ weeks of age) feeding study. Feed consumption and egg weight were significantly (P<0.01) higher at barn than at cage. However, there were no significant differences between two housing systems on hen day egg production (HDEP), egg mass, body weight, feed efficiency and livability. In case of egg quality parameters, shell thickness, albumen height and Haugh unit were significantly (P<0.05) different between two systems. Haugh unit and albumen height were significantly higher in eggs produced at cage (88.97 and 8.16 mm) compared than those produced at barn (83.11 and 6.87 mm), whereas shell thickness was thicker in eggs produced at barn than those produced at cage. Shape index, breaking strength, blood spots and yolk index data were not influenced significantly by the types of housing system. To implement welfare bestowing production, this study showed that the barn system can replace the conventional cage without serious sacrifices on starting phase egg production. In addition, this study suggested that the barn system need to be optimized in view of daily feed consumption.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1623-1628
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• 2011

The effects of dietary lysine (Lys) at a fixed ratio to crude protein (CP) and essential amino acids (EAA) on early growth response of broilers were studied. Four diets were formulated to contain similar metabolizable energy (ME, 2,950 kcal/kg) but contained graded levels of incremental Lys (1.1, 1.2, 1.3 and 1.4%) while also increasing the dietary CP and EAA (methionine, methionine+ cystine, threonine and tryptophan) to maintain a constant ratio with Lys. Each diet was fed at random to 10 replicates of 6 chicks each throughout the experimental period (1-21 d). At the lowest concentration of Lys of 1.1% (19.04% CP), body weight gain (BWG) was lowest and feed conversion ratio (FCR) was poorest. The BWG increased and FCR decreased linearly as dietary Lys increased upto 1.3% (22.5% CP). Lowest feed consumption was observed in the dietary group that contained 1.1% Lys (19.04% CP) in the diet. Increasing the concentration of Lys to 1.2% (20.77% CP), significantly increased the feed consumption. The concentrations of protein, calcium, phosphorus and cholesterol in serum were not influenced by the variation in Lys contents in the diet. The humoral immune response as measured by antibody titre in response to SRBC inoculation was significantly lower in the diets containing 1.1% Lys compared to 1.4%. It is concluded that the Lys requirement of broilers is 1.3% (22.5% CP) during 0 to 21 days of age for eliciting optimum performance when a fixed ratio of Lys to CP (1:17.31) and essential AA is maintained (1:0.47 Met; 1:0.56 Thr; 1:0.17 Try).

• Korean Journal of Poultry Science
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• v.27 no.1
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• pp.13-18
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• 2000

An experiment was conducted to evaluate feeding values of food and other organic wastes, and to determine their dietary effects on performance and carcass yield in ducks. A total of 156 meat type ducklings at the age of 25 days were housed in 12 pens and assigned to 4 treatments, in which the birds were fed for 21 days. One of 4 diets, commercial duck feed, raw food waste (RFW), fermented food waste(FFW) and mixture of 50% RFW and 50% FFW (R+FFW). Feed consumption was significantly higher (p＜0.05) in the RFW groups. Body weight gain showed no significant differences between the control and other treatments. The feed consumption ratio was significantly higher in the RFW groups (p＜0.05) than that of the other groups. Carcass yield was significantly higher in the R+FFW groups (p＜0.05) than the other groups. The weight of liver per live body weight showed significant differences among the treatment groups (p＜0.05). The length of intestine was significantly different from those of the treated groups(p＜0.05).

• Microbiology and Biotechnology Letters
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• v.28 no.1
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• pp.8-13
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• 2000

A study was carried out to determine the probiotic effect of Lactobacillus reuteri BSA-131 by investigating the growth performance and fecal microbial population of piglets. Five dietary treatment groups, the basal diet (control, BD), basal diet with antibiotics(BA), basal diet with 2$\times$106/g of probiotics (BP6), 2$\times$108/g of probiotics (BP8) and basal diet with antibiotics and 2$\times$108/g probiot-ics(BAP8) were divised. Each dietary treatment group was consisted of 1 month of age piglets(male 13, female 12). Fecal micro-flora, body weights and feed consumption were measured at before, after and stop feeding of probiotics. The results showed that the CFU of fecal Enterobacteriaceae of piglets of the group BA, BP6, BP8 and BAP8, were reduced (P<0.05) compared to control BA. On the contrary, Lactobacillus counts were increased significan시 (P<0.001) in all groups fed probiotics dites, but not antibiotics. Body weight of probiotics treated piglets were improved 5% (p<0.001) in BP6 group than that of control group and antibiotic treated piglets BAP group was 27% (P<0.001) higher than BA group. The amount of feed consumption value of probiotics treated piglets showed 21-30% (P<0.001) lower intake than the control group, whereas antibiotic treated piglets BAP was 20% (P<0.001) higher than BA group. The results showed that body weights and feed to gain ratios were improves 19% when compared to control piglets for groups fed diets probiotic. It is very suggestive that productivity of probiotic piglets would be economical in pig farming.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.193-199
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• 2001

Twenty-four new born crossbred (Bos indicus$\times$Bos taurus) calves were distributed in two equal groups and assigned to two different pre-starter diets with (Group 1) and without (Group 2) fish meal to study the effect of replacement of animal protein by vegetable protein in the diet and the age of animals on ruminal metabolic development. All calves were fed colostrum for 24 h and whole milk until weaning at 8 weeks of age. Rumen fluid samples were collected on 4 d, 1 wk, and then weekly interval up to 8 wk of age. Rumen fluid samples were analysed for pH, TVFA, lactic acid and N fractions (total N, total soluble N, trichloro acetic acid (TCA) soluble N, TCA precipitable N and ammonia N). Weekly feed intake and live weight gain pattern showed an increasing trend with the advancement of age, but were similar in both groups. The pH fell steadily during 0-4 wk of age and then stabilized in later period. A close relationship (r=0.80) between starter intake and TVFA concentration was observed in both the groups. Lactic acid (meq/l) and ammonia N (mg/dl) concentration showed initial rise (0.55 and 14.97 on day 4 to 3.38 (7 wk) and 32.85 (4 wk), respectively) to fall (2.74 and 17.60) again during 8 wk of age in response to increase in dry feed consumption (10% initially to 83% of diet dry matter at 8 wk of age). The TCA precipitable fraction of N did not show any change during 0-8 wk of age. Data indicate that the metabolic changes responded rapidly to dry feed intake which did not differ in fish meal and non-fish meal groups, and a poor voluntary consumption of oat hay retards the progressive changes in live weight and rumen microbial development.

• Animal Bioscience
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• v.34 no.7
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• pp.1202-1209
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• 2021

Objective: The intestinal microbiota plays an important role in host physiology, metabolism, immunity, and behavior. And host genetics could influence the gut microbiota of hybrid animals. The three-way cross model is commonly utilized in commercial pig production; however, the use of this model to analyse the gut microbial composition is rarely reported. Methods: Two three-way hybrid pigs were selected, with Saba pigs as the starting maternal pig: Duroc× (Berkshire×Saba) (DBS) pig, Berkshire×(Duroc×Saba) (BDS) pig. One hundred pigs of each model were reared from 35 days (d) to 210 d. The body weight or feed consumption of all pigs were recorded and their feed/gain (F/G) ratio was calculated. On day 210, 10 pigs from each three-way cross were selected for slaughter, and cecal chyme samples were collected for 16S rRNA gene sequencing. Results: The final body weight (FBW) and average daily gain (ADG) of DBS pigs were significantly higher than those of BDS pigs (p<0.05), while the F/G ratios of DBS pigs were significantly lower than those of BDS pigs (p<0.05). The dominant phyla in DBS and BDS pigs were Bacteroidetes (55.23% vs 59%, respectively) and Firmicutes (36.65% vs 34.86%, respectively) (p>0.05). At the genus level, the abundance of Prevotella, Roseburia, and Anaerovibrio in DBS pigs was significantly lower than in BDS pigs (p<0.01). The abundance of Eubacterium, Clostridium XI, Bacteroides, Methanomassiliicoccus, and Parabacteroides in DBS pigs was significantly higher than in BDS pigs (p<0.05). The FBWs and ADGs were positively correlated with Bacteroides, ClostridiumXI, and Parabacteroides but negatively correlated with the Prevotella, Prevotella/Bacteroides (P/B) ratio, Roseburia, and Anaerovibrio. Conclusion: These results indicated that host genetics affect the cecal microbiota composition and the porcine gut microbiota is associated with growth performance, thereby suggesting that gut microbiota composition may be a useful biomarker in porcine genetics and breeding.