• Title/Summary/Keyword: faucet cleanliness

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Improvement of sampling method for bacteriological test in tap water (수도꼭지에서 미생물 검사 목적의 샘플링 방법 개선 연구)

  • Lee, Eun-Sook;Lee, Dong-Sik;Lee, Mok-Young;Lee, Man-Ho;Han, Sun-Hee
    • Journal of Korean Society of Water and Wastewater
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    • v.25 no.4
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    • pp.519-525
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    • 2011
  • We studied on the effect of faucet cleanliness, faucet conditions (screen, mixing faucet), and flushing time for bacterial detection in tap water. As results, tap water should be left to run to waste for 2 to 3 minutes and if a questionable cleanliness is questionable, disinfect the faucet by using flaming or other methods before sampling. We proposed sampling method to decrease effect of factors associated with bacterial detection in tap water and contributed to be evaluated more accurate water quality.

Study on the Chlorine-Resistant Bacteria Isolated from Water Pipe Network (상수도관망에서 분리한 잔류염소 내성균에 관한 연구)

  • Hyun, Jae-Yeoul;Yoon, Jong-Ho
    • Journal of Korean Society on Water Environment
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    • v.27 no.3
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    • pp.334-341
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    • 2011
  • The free residual chlorine of tap water samples, collected from 266 faucets on the water pipe network in Daegu City, was between 0.1 and 0.79 mg/L. On microorganic tests, general bacteria and the coliform goup were not detected and thus the tap water was turned out to be fit to drink. In particular, samples of which free residual chlorine was 0.1 mg/L and over were cultured in R2A agar media at $25^{\circ}C$ for 7 days, and as a result heterotrophic bacteria were detected in 65.9% of samples; (1). The closer tap water got to the faucet from the stilling basin, the lower residual chlorine concentration became but the more the bacterial count became. And, more bacteria were detected in the R2A agar medium than in the PCA medium. (2). In the case of separated strains, most colonies were reddish or yellowish. 16S rRNA sequence was identified as Methylobacterium sp. and Williamsia sp., and yellow strain was identified as Sphingomonas sp., Sphingobium sp., Novosphingobium sp., Blastomonas sp., Rhodococcus sp. and Microbacterium sp. White strain was identified as Staphylococcus sp. (3). Sterilized tap water in polyethylene bottles was inoculated with separated strain and was left as it was for 2 months. As a result, bio-film was observed in tap water inoculated with Methylobacterium sp. and Sphingomonas sp. It was found that heterotrophic bacteria increased when free residual chlorine was removed from tap water in the water pipe network. Thus, there is a need to determine a base value for heterotrophic bacteria in order to check the cleanliness of tap water in the water pipe network.