• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.51-54
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• 2007

Soybean has a morphological type with a broadened and flattened stem. Fasciation has been suggested as a new gene for soybean research. SSR marker linked to the $\Large f$ locus that controls fasciation phenotype has not identified within 10 cM. A mapping population consisting of 94 $F_2$ progenies was derived from a cross between wild type Clark (FF) and fasciation mutant C32 (${\Large f}{\Large f}$). The phenotype of $F_2$ individual plants was recorded at R2 and R3 growth stage from field. One-thousand 10-mer oligonucleotide RAPD primers and 29 SSR primers selected from the D1b+W of the soybean molecular linkage map were used. A genetic map was constructed from the segregating 35 RAPD, four SSR markers and one phenotypic(wild type/fasciation) marker. The segregation ratios of 3 : 1 observed in the $F_2$ population and the Chi-square values strongly suggest that the fasciation trait is controlled by a single recessive gene. Satt537 marker was linked to $\Large f$ locus at a distance of 9.6 cM. Assignment of the $\Large f$ locus to linkage group D1b+W and identification of markers can be used as an initial step for fine mapping of the $\Large f$ gene.

• Research in Plant Disease
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• v.29 no.1
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• pp.39-44
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• 2023

To diagnose lily fasciation, lily bulbs showing fasciation were collected from several greenhouses in Jeju Island, South Korea. Bacteria were isolated from the lily bulbs and amplified with both primers for fasA in plasmid and for putative glycosyltransferase epsH gene in chromosome of Rhodococcus fascians. Three bacterial isolates were detected with the P450 primer set and identified as R. fascians by NCBI blast analysis. Twelve bacterial isolates were identified as R. fascians using RS02785 primer set, including the three bacterial isolates identified as the same pathogen using the P450 primer set. Pathogenicity of these bacterial strains identified as R. fascians was demonstrated. Apparent symptoms were observed on wounded lily leaves after inoculation with each bacterial suspension whereas no symptom was found on lily leaves treated with H₂O. Furthermore, bacteria re-isolated from wounded sites were identified as R. fascians. Based on the results, these two sets of primers are recommended for quarantine of R. fascians.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.714-719
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• 2013

The bent peduncle phenomenon (BPP) is known as a kind of physiological disorders found in cut rose plants, which causes the reduction of cut flower yields. As yet unknown mechanisms, however, it has not been easy to find solutions for BPP. To address this challenge, this study was conducted to investigate the characteristics of floral morphology, growth, and endogenous phytohormone level of BPP shoots in cut rose plants (Rosa hybrida L. 'Beast'). Morphological observation confirmed the fact that BPP was accompanied by the early formation and being phyllody of a specific sepal among five sepals, which gave rise to peduncle bending in the upper part of the phyllody with fasciation. Year-round BPP frequency in 'Beast' cultivar was in the range of 5 to 20% with seasonal change, increasing rapidly with an average temperature rise in summer. Except bent peduncle, the BPP shoots appeared to grow normally in terms of speed and size of floral development in comparison with normal. However, carbohydrate distribution to the floral part in BPP shoots was significantly reduced. The level of endogenous IAA (3-Indoleacetic acid) within the floral part in BPP shoots was highly maintained during floral development, in contrast to normal shoots. $GA_3$ contents were not significantly different between normal and BPP shoots. This study indicates that BPP would be induced by a series of courses: abnormally early formation of a specific sepal relative to high temperature, being phyllody of the sepal with fasciation, and continuous supply of endogenous IAA by phyllody.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.167-172
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• 1986

This experiment was carried out to obtain some information about the growth habit and morphological and anatomical characteristics of fasciated soybean, var. Buchai-kong. The results obtained are summarized as follows: 1. Main stern of fasciated soybean, var. Buchai-kong was gradually broadened and became flat, on which leaves emerged irregularly compared with normal soybean, var. Danyeop-kong. The number of leaves on main stern was more in Buchai-kong, but the total number of leaves per plant was not considerably different each other. 2. The degree of fasciation (max./min. in diameter at the broadened part) in main stern of Buchai-kong was about 3.1, and was related to the amount of growth of main stern and branches of Danyeop-kong. Stern fasciation types found in Buchai-kong were linear (the most common) and bifurcated with regard to branching of main stern tip. 3. The number of branches per plant was much less in Buchai-kong than in Danyeop-kong. There were fas-ciated branches and fasciated petiole in Buchai-kong. 4. About 75 percent of pods of Buchai-kong were concentrated on the top of stern, which showed strong pod clustering. 5. Seed yield per plant of Buchai-kong was about 60 percent of that of Danyeop-kong, and the decrease in seed yield was mostly affected by the decrease in seed weight and seeds per pod. 6. The number of vascular bundles of transection of mature stern was more in Buchai-kong, but Danyeop-kong developed the better vascular bundle.

• Research in Plant Disease
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• v.17 no.3
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• pp.265-271
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• 2011

Seven phytoplasma diseases have been occurred on floricultural crops in Korea : Ph-ch1 and Ph-ch2 of chrysanthemum, Ph-lily of lily, petunia flat stem-Korean (PFS-K) of petunia, poinsettia branch inducing- Korean (PoiBI-K) of poinsettia, statis witches' broom-Korean (SWB-K) of statis and azalea witches broom (AWB). Classification of the seven phytoplasmal diseases based on 16S ribosomal RNA (rRNA) sequences showed that floricultural crop phytoplasma disease were widespread in order of aster yellow (AY), stolbur and X-disease in Korea. In phenotypic characters, the fasciation was occurred in both monocotyledon plant of lily and dicotyledon plants of petunia and poinsettia. Besides, the fascination was occurred in Ph-lily of stolbur, petunia PFS-K of AY and PoiBI-K of X-disease. This result indicated that phytoplasma classification based on 16S rRNA and symptoms are not consistently related. The comparison of 16S rRNA sequence of the seven floricultural crop phytoplasma with five tree phytoplasmal diseases of jujube witches' broom, paulownia witches' broom, wild jujube witches' broom, mulberry dwarf, golden rain phytoplasma occurred in Korea showed as high as 88.5-99.9% homology. Among them, especially mulberry dwarf showed the highest homology with the seven floricultural crop phytoplasms. Based on this result, floricultural crop phytoplasmas were assumed to be transmitted by insect vectors from tree phytoplasmas in Korea.