• 미생물학회지
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• 제51권2호
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• pp.148-155
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• 2015

세균 16S rRNA 유전자 특이 프라이머를 기초로 하여 중합 효소연쇄반응을 이용하여 Pleurotus eryngii (큰느타리 버섯)의 내부에 존재하는 세균의 다양성을 조사하였다. 세균 16S rRNA의 라이브러리는 버섯 갓(BC, body cap)와 줄기(BS, body stipe)로 구성하였다. BC 20 클론은 네 그룹으로 구별되었고 가장 큰 그룹은 Firmicutes (클론의 40%) 있었다. 그러나, BS 20 클론은 여섯 그룹으로 나뉘어졌고 가장 큰 그룹은 Actinobacteria (클론의 40%) 있었다. 전체 버섯 내에 존재하는 세균 그룹은 그람양성 세균(62.5%) 있었다.

• 인간식물환경학회지
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• 제24권2호
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• pp.137-152
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• 2021

Background and objective: This study was carried out to investigate and analyze the preference for plant fragrance and the psychological effects of a agro-healing activity in 101 firefighters aged 39.71 ± 10.94. Methods: For the psychological evaluation, post-traumatic stress symptoms, Korean occupational stress scale short form (KOSS-SF), emotional intelligence, and job satisfaction were surveyed on the Likert scale. For the preference for plant fragrances, 20 ㎕ of commercially available essential oils from four plant species (Mentha spp., Lavandula spp., Citrus limon, C. sinensis) were absorbed into each test paper and provided to the participants. Results: The rate of post-traumatic stress symptoms was high at 37.6% of the firefighters surveyed.. C. limon fragrance had the highest preference among the four plant fragrances, followed by C. sinensis > Mentha spp. > Lavandula spp. with statistically significant difference (F = 14.256, p < .001). The lower their age, income, and position, and higher the education level, the higher the preference for plant fragrances. And the administrative group had higher preference for plant fragrances than field group. In addition, as a result of examining the correlation between job satisfaction, preference for plant fragrance, post-traumatic stress, emotional intelligence, and job stress for fire-fighters, the higher the job satisfaction, the higher the fragrance preference and emotional intelligence, and the lower the post-traumatic stress and job stress. Conclusion: Therefore, installing a garden using botanical fragrances at workplaces that firefighters can easily access will improve job satisfaction and relieve stress. It can be concluded that applying the fragrance of plants to the healing farming activities at the fire-fighter's workplace can increase the usefulness of the healing activities.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1553-1558
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• 2012

This study was conducted to investigate the effects of stocking density or group size on feed intake, daily gain, and carcass characteristics of Hanwoo (Korean indigenous breed) steers reared from 7 months to 31 months of age. Thirty Hanwoo steers were divided into four groups with three replicates each (a total of 12 pens). In each group, one (G1), two (G2), three (G3), and four steers (G4) per pen were allocated as treatments. Pen size was $32.0m^2$, and therefore Hanwoo steers in G1, G2, G3, and G4 were reared under different space allowances, i.e. 32.0, 16.0, 10.6, and $8.0m^2$/steer, respectively. Steers were reared following a conventional beef cattle management method in Korea, and were offered a fixed amount of commercial concentrate with ad libitum forages. Results were subjected to analysis of variance with stocking density as the main effect, and significance was declared at p<0.05. Although total feed intake was not significantly altered, it numerically increased in animals of low stocking density (G1) compared to those subjected to high stocking density treatment (i.e. G4). Feed conversion ratio was higher (p<0.05) in G3 compared to G1 and G2. Animals in G1 (low stocking density) grew faster (p<0.05) than those of high stocking density (G3 and G4). Back fat thickness, meat yield index, and meat yield grade were similar among all levels of stocking density. However, longissimus muscle area was larger in G1 and G2 (p<0.01) compared to G3 and G4, and animals in G3 produced smaller carcasses (p<0.05). Carcass quality traits, including marbling score, meat color, fat color, texture, maturity and meat quality grade, as determined by a group of experts, were not significantly different among the treatments. In conclusion, lower stocking density resulted in increased feed efficiency, daily gain, and carcass weight in Hanwoo steers. However it remains unclear whether such differences are the results of stocking density or group size, or a combination of both. Nonetheless, these results confirm previous studies reporting a negative effect of increased stocking density on animal productivity. Further, animal welfare under an intensive farming system in relation to economical return is discussed.

• 생명과학회지
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• 제21권6호
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• pp.884-892
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• 2011

본 연구는 흑마늘 숙성기간 중의 흑마늘의 지표물질이라 할 수 있는 S-allylcysteine (SAC)의 함량을 측정한 다음 흑마늘 추출물이 고지방식이로 유발된 흰쥐에 미치는 영향을 조사하였다. 흑마늘을 10일, 15일 및 20일 숙성 중 S-allylcysteine의 함량은 15일 일 때가 가장 높았으며, 관능검사 결과도 숙성 15일째가 좋았다. 고지방 식이만 공급된 동물군을 정상군, 위약군 그리고 고지방식이에 흑마늘 추출물의 첨가 농도에 따라 저농도군, 중농도군 및 고농도군으로 5그룹으로 나누어 6주간 사육하였다. 정상군, 실험군 모두 체중은 지속적으로 증가하였으나 그룹 간의 유의적 차이는 없었고 흑마늘 추출물 첨가 급이군이 정상군에 비하여 체중 및 식이 효율에 유의적 차이는 없었다. 혈중 총지질은 흑마늘 추출물의 첨가 농도에 관계없이 감소하였으며, 총 콜레스테롤 및 중성지방도 흑마늘 추출물의 첨가 농도가 높을수록 유의적으로 감소하였다. 흑마늘 추출물 급이에서도 정상군에 비하여 HDL-콜레스테롤이 유의적으로 증가하였고 LDL-콜레스테롤은 감소하였다. 간의 총지방, 총 콜레스테롤 및 중성 지방의 함량은 정상군에 비하여 흑마늘 추출물을 급이한 저농도, 중농도, 고농도 군에서 모두 감소되는 경향을 나타내었다. 이상의 실험결과를 보면, 6주 동안 고지방식이로 유도된 고 콜레스테롤혈증은 동물 실험에서 흑마늘 추출물 첨가가 저농도, 중농도 및 고농도에서 혈액과 간의 중성 지방과 총콜레스테롤을 감소하는 효과가 있었으므로 체내지질 개선효과를 갖는 식품 소재로서의 가능성이 있음을 확인할 수 있었다.

• 보건교육건강증진학회지
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• 제17권1호
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• pp.131-154
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• 2000

This study was conducted to provide basic data for development of public information program and public policy which could prevent the medicine abuse in Korea, examining the level of knowledge of medicine and its related factors. Data were collected from the 2,011 residents who live in mtropolitan cities, large-sized cities, small and medium cities, and small towns The results of this study are summarized as follows. 1) In case of purchasing of medicines in pharmacy, 67.3％ of the respondents chose the medicines through recommendations of the professionals such as pharmacists and doctors, while 32.7％ of the respondents chose the medicine through self-judgement, advertizing, or recommendation of relative. 2) 64.7％ of the respondents obtained the information on medicines through TV. It appeared to be higher in the groups of female of the twenties, the unmarred, a brother and sister threesome, highschool graduates, housewives, residents in small and medium cities, atheists, and the middle class, displaying the significant difference from the other groups. 3) 40.5％ of the respondents recognized the side effect of the medicine when they took the medicine, while 34.4％ did not recognize it. The rate of experience in the side effect was 39.7％. The informations on the medicine abuse and the risk of addiction were obtained through broadcast media (47.9％), publications (12.1％), and health professionals (11.6％). 4) 81.1％ of the respondents experienced taking of the fatigue relieving medicine. The experience in taking of the fatigue relieving medicine appeared to be higher in the groups of the forties. the married. a brother and sister threesome. highschool graduates. persons engaging in farming, livestock raising, and forestry, the residents in small towns, and Christians. Each group displayed the significant difference from the other groups. 5) According to the level of knowledge of medicines, the respondents marked average 11.7 ± 3.76 points on the base of 24 points. It appeared to be higher in the groups of female of the twenties, a brother and sister foursome, college graduates, teachers, Catholics, and the middle class, displays the significant difference from the other groups. 6) According to the experience in taking of health medicine and health food, 81.1％ of respondents had the experience in taking ‘the fatigue relieving medicine’; 72.4％ ‘carrot or vegetable juice’; 69.5％ ‘ginseng’; 63.0％ ‘mushroom’; 42.5％ ‘dog meat’; 38.0％ ‘aloe’; 36.4 ‘deer antlers’; 11.4％ ‘snake’; 2.0％ ‘the penis of a fur seal’. 7) The factors influencing the level of knowledge of medicine include experiences in taking of the tonic, the fatigue relieving medicine, and the nutritive medicine, economic status, the number of brothers and sisters, education level, marital status, father's education level, and age. The factors influencing the experience in side effect of medicine are experiences in taking of the fatigue relieving medicine, the nutritive medicine, and the tonic, sex, age, education level, father's education level, marital status, economic status, religion, and the number of brothers and sisters. In conclusion, it is estimated that the level of knowledge of medicines is significantly low in Korea. Especially, it is found out that workmen, students, the upper class, the class of low education level, and persons engaging in farming, livestock raising, and forestry neglect importance of knowledge of medicine. Therefore, it is necessary for public authority, associations related, and health professionals to develop programs for public information and education to help people obtain basic knowledge of medicine.

• 농촌의학ㆍ지역보건
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• 제40권4호
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• pp.228-239
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• 2015

본 연구는 2013~2014년도 한양대학교 농업안전보건센터 현장검진에 참여한 경기 일부 지역 농업인을 대상으로 급성 농약중독 증상 유병률과 이와 관련된 위험요인을 규명하고자 하였다. 연구 결과, 급성 농약중독은 농업종사기간, 농약 살포 년수, 연간 농약 살포일수, 평생 농약 살포일수가 길수록, 혼합 농약 사용 개수가 많을수록, 노지 재배를 하는 경우 위험도가 증가하는 것으로 나타났다. 향후, 급성 농약중독에 대한 취약 집단을 좀 더 체계적으로 관리 및 교육할 수 있는 방안의 마련이 필요할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권11호
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• pp.1643-1650
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• 2017

Objective: The control of psychrotrophic bacteria causing milk spoilage and illness due to toxic compounds is an important issue in the dairy industry. In South Korea, Gangwon-do province is one of the coldest terrains in which eighty percent of the area is mountainous regions, and mainly plays an important role in the agriculture and dairy industries. The purposes of this study were to analyze the indigenous microbiota of raw milk in Gangwon-do and accurately investigate a putative microbial group causing deterioration in milk quality. Methods: We collected raw milk from the bulk tank of 18 dairy farms in the Hoengseong and Pyeongchang regions of Gangwon-do. Milk components were analyzed and the number of viable bacteria was confirmed. The V3 and V4 regions of 16S rRNA gene were amplified and sequenced on an Illumina Miseq platform. Sequences were then assigned to operational taxonomic units, followed by the selection of representative sequences using the QIIME software package. Results: The milk samples from Pyeongchang were higher in fat, protein, lactose, total solid, and solid non-fat, and bacterial cell counts were observed only for the Hoengseong samples. The phylum Proteobacteria was detected most frequently in both the Hoengseong and Pyeongchang samples, followed by the phyla Firmicutes and Actinobacteria. Notably, Corynebacterium, Pediococcus, Macrococcus, and Acinetobacter were significantly different from two regions. Conclusion: Although the predominant phylum in raw milk is same, the abundances of major genera in milk samples were different between Hoengseong and Pyeongchang. We assumed that these differences are caused by regional dissimilar farming environments such as soil, forage, and dairy farming equipment so that the quality of milk raw milk from Pyeongchang is higher than that of Hoengseong. These results could provide the crucial information for identifying the microbiota in raw milk of South Korea.

• 식물병연구
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• 제15권2호
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• pp.63-67
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• 2009

벼 줄무의잎마름병은 Tenuivirus 그룹에 속하며, 애멸구가 매개하는 바이러스병으로 우리나라에서는 2001년, 2007년 대발생한 보고가 있다. 벼 줄무의잎마름병의 중간기주조사를 위하여 2008년 3월부터 9월까지 전국 13지역 26지점의 논 주위 발생 잡초 15과 50종을 채집하였다. 보리, 뚝새풀, 들묵새, 잠자리피, 돌피, 바랭이에서 벼 줄무의잎마름병의 이병을 확인하였다. 들묵새는 가을에 발아하여 봄에 고사하는 겨울나기 외래잡초로 현재 녹비 및 피복작물로 많이 사용되고 있다. 들묵새의 지역별 이병률조사를 위하여 2008년 6월 부안군 계화면 시료 111주, 서천군 마서면 시료 50주를 ELISA한 결과 부안군은 32주가 감염이 확인되어 28.8%의 이병률을, 서천군은 8주가 감염이 확인되어 16.0%의 이병률을 확인할 수 있었다. 현재까지 들묵새의 벼 줄무의잎마름병의 감염에 관련된 보고가 없었으며, 이 논문에서 벼 줄무의잎마름병의 중간기주로 들묵새를 최초 보고한다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.903-910
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• 2015

To maintain a predominantly pasture-based system, the large herd milked by automatic milking rotary would be required to walk significant distances. Walking distances of greater than 1-km are associated with an increased incidence of undesirably long milking intervals and reduced milk yield. Complementary forages can be incorporated into pasture-based systems to lift total home grown feed in a given area, thus potentially 'concentrating' feed closer to the dairy. The aim of this modelling study was to investigate the total land area required and associated walking distance for large automatic milking system (AMS) herds when incorporating complementary forage rotations (CFR) into the system. Thirty-six scenarios consisting of 3 AMS herds (400, 600, 800 cows), 2 levels of pasture utilisation (current AMS utilisation of 15.0 t dry matter [DM]/ha, termed as moderate; optimum pasture utilisation of 19.7 t DM/ha, termed as high) and 6 rates of replacement of each of these pastures by grazeable CFR (0%, 10%, 20%, 30%, 40%, 50%) were investigated. Results showed that AMS cows were required to walk greater than 1-km when the farm area was greater than 86 ha. Insufficient pasture could be produced within a 1 km distance (i.e. 86 ha land) with home-grown feed (HGF) providing 43%, 29%, and 22% of the metabolisable energy (ME) required by 400, 600, and 800 cows, respectively from pastures. Introduction of pasture (moderate): CFR in AMS at a ratio of 80:20 can feed a 400 cow AMS herd, and can supply 42% and 31% of the ME requirements for 600 and 800 cows, respectively with pasture (moderate): CFR at 50:50 levels. In contrast to moderate pasture, 400 cows can be managed on high pasture utilisation (provided 57% of the total ME requirements). However, similar to the scenarios conducted with moderate pasture, there was insufficient feed produced within 1-km distance of the dairy for 600 or 800 cows. An 800 cow herd required 140 and 130 ha on moderate and high pasture-based AMS system, respectively with the introduction of pasture: CFR at a ratio of 50:50. Given the impact of increasing land area past 86 ha on walking distance, cow numbers could be increased by purchasing feed from off the milking platform and/or using the land outside 1-km distance for conserved feed. However, this warrants further investigations into risk analyses of different management options including development of an innovative system to manage large herds in an AMS farming system.

• 한국산업보건학회지
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• 제22권4호
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• pp.337-344
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• 2012

Objectives: This study was conducted to assess the trend of work-related accident rate in the past 10 years (2002~2011) in Gusan district, Ministry of Employment and Labor, Korea. Methods: To analyze the change of work-related accident rate in the past 10 years in Gunsan District, we analyzed the work-related accident and disease by the type of business, business scale and analyzing the victim's age and job period using workplace information management system(PKMS) in the Ministry of Employment and Labor. Results: The average rate of work-related accidents in Gunsan district was 0.96% in the past 10 years and it was a little higher than that of national average 0.75%. The rates of work-related accidents tended to decrease from 1.11% in 2002, to 1.09% in 2006, and to 0.79% in 2011. Mortality (per 10,000) rates also tended to decrease in the years 2002, 2006, and 2011 as 3.07, 2.46, and 1.53. By the type of business, the average rate of work-related accidents in the past 10 years was the highest in agriculture as 1.77%, followed by forestry farming at 1.76%. By the business scale, the average rate of work-related accidents was the highest in the group of under 5 employees as 2.05% and it was the lowest in the group of more than 300 employees as 0.50%. The ratio of work-related accidents in the 30's age group was decreased from 41.7% in 2002 to 25.2% in 2011 and the ratio for the 50's age group was increased from 28.6% in 2002 to 49.5% in 2011. Conclusions: Although the rate of work-related accident was decreased in the past 10 years, the polarization of work-related accidents rated by the business scale and age groups show a significant difference. So it is needed that the planning and implementing of work-related accidents prevention policies.