Since 1997, Korean Ministry of Knowledge Economy and Ministry of Environment have established data on some 400 basic raw and subsidiary materials and process like energy, petro-chemical, steel, cement, glass, paper, construction materials, transportation, recycling and disposal etc by initiating establishment of LCI database. Regarding agriculture, Rural Development Administration has conducted establishment of LCI database for major farm products like rice, barley, beans, cabbage and radish etc from 2009, and released that they would establish LCI database for 50 items until 2020 later on. The domestic LCI database for seeds, seedling, agrochemical, inorganic fertilizer and organic fertilizer etc is only at initial stage of establishment, so overseas LCI databases are brought and being used. However, since the domestic and overseas natural environments differ, they fall behind in reliability. Therefore, this study has the purpose to select organic farming materials, survey the production process for various types of organic farming materials and establish LCI database for the effects of greenhouse gas emitted during the process in order to select carbon basic units for agricultural production system compliant in domestic situation instead of relying on overseas data and apply life cycle assessment of greenhouse gas emitted by each crop during the process. As for selecting methods, in this study organic farming materials were selected in the method of direct observation of material and bottom-up method a survey method with focus on the organic farming materials admitted into rice production. For the basic unit of carbon emission amount by the production of 1kg of organic farming material, the software PASS 4.1.1 developed by Korea Accreditation Board under Ministry of Knowledge Economy was used. The study had the goal to ultimately provide basic unit to calculate carbon emission amount in executing many institutions like goal management system and carbon performance display system etc in agricultural sector to be conducted later on. As a result, emission basic units per 1kg of production were calculated to be 0.0088kg-$CO_2$ for charcoal, 0.1319kg-$CO_2$ for grass liquid, and 0.2804kg-$CO_2$ for microbial agent.
The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, 'Seolgang' was developed by crossing two monokaryons 'CM020913-27' and 'SSU423-31'. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of 'Seolgang' on CDA was better at $20^{\circ}C$ and $25^{\circ}C$ when it was compared with that of '505 Ho'. The mature cap shape of new strain 'Seolgang' is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain '505 Ho', the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of '505 Ho'. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of '505 Ho'. Genetic analysis of the new strain 'Seolgang' showed different profiles compared to '505 Ho', CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.
This survey was carried out to investigate the dimensions of farrowing accommodation and farrowing crate by types. Farrowing crates are used to prevent the crushing death in the swine industry generally but farrowing crates include many problems in the animal welfare viewpoint: e.g. restriction of movements and maternal instincts. Therefore, the studies of alternative systems have been discussed for a while in U.K., U.S.A. and Canada. We surveyed eight farms for about eight months. To accurately compare the farrowing housing, small scale swine farms (3,000hd or less) were excluded from all data in this survey. Lactating accommodations were divided into vertical lactating accommodation type and diagonal accommodation type, and farrowing crates were divided into vertical bar type and horizontal bar type. In this survey, nipple waterer, partial slotted floor and total slotted floor were 80%, 40% and 60% respectively, and the lactating accommodations' dimensions ranged from 3.90 to $4.92\;m^2$. We believe that these data of the survey contribute to develop alternative systems and then contribute to improve the animal welfare in Korea.
This study aimed to evaluate the prevalence of Listeria monocytogenes on livestock farms in Korea and determine their serotypes and genetic correlations. Twenty-five livestock farms in Korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. Samples were enriched in LEB (Listeria enrichment broth) and Fraser broth media, and then plated on Palcam agar. The isolates were identified by PCR and 16S rRNA gene sequencing. Then, the sero-types, presence of virulence genes (actA, inlA, inlB, plcB, and hlyA), and antibiotic resistance were determined. Genetic correlations among the isolates were evaluated by analyzing the restriction digest pattern with AscI. Of the 2,018 samples, only 3 (0.15%) soil samples (FI-1-FI-3) from 1 farm in the south east region were positive for L. monocytogenes. Based on biochemical tests and multiplex PCR, the serotype of the isolates were 4ab (FI-1 and FI-3) and 3a (FI-2), which are not common in foodborne L. monocytogenes. The 3a sero-type isolate was positive for all tested virulence genes, whereas the 4ab serotype isolates were only positive for hlyA, actA, and inlA. The isolates were resistant to all 12 tested antibiotics, especially FI-3. The genetic correlations among the isolates were 100% for those of the same serotype and 26.3% for those of different serotypes. These results indicate that the prevalence of L. monocytogenes on livestock farms in Korea is low; however, the isolates are pathogenic and antibiotic resistant.
This study aims to economically evaluate rice production and various ecological services provided by organic rice paddy carrying out rice-fish mixed farming. It was also conducted to find a stable structure for increasing the income of rice-producing farmers and promoting ecological services by evaluating the economic feasibility of organic rice paddy for rice-fish mixed farming. As a result of the analysis of expected effects by ecological service functions according to the types of agriculture in the rice-fish mixed farming, general conventional paddy fields were evaluated to have an effect of continuously decreasing biodiversity such as amphibian reptiles, aquatic insects, bird habitats, experience and ecological education, and vegetation diversity, while the biodiversity of organic and rice-fish mixed paddy fields was assessed to increase. As a result of evaluating the economic value, as the area for fish production increases compared to the common practice and organic paddy fields, rice production decreases, but fish production increases and total income was analyzed to increase. In addition, if the value of ecosystem service functions is assessed in the future, it will increase further. It is hoped that these findings will be used as basic data for solving oversupply problems in the rice industry, preserving safe farm income, and improving sustainable farming and ecosystem service functions.
Since Tongil has been cultivated widely in farmers' fields, the variety is known to be highly resistant to existing races of Pyricularia oryzae in Korea. However, Tongil and the sister lines were moderately susceptible to race IA-65, which was isolated from Tongil at the blast nursery of the College of Agriculture, Seoul National University, 1972. In field tests of 1973, pathogenic reactions of IR lines at the isolated nursery, where IA-65 had been inoculated, were significantly higher than those at the farm nursery. When seedlings of 196 IR lines were inoculated with IA65, about $30\%$ of the IR lines including IR667-98 were moderately susceptible. Among the 30 isolates of P. oryzae obtained mostly from indicas, 12 were also differentiated in 1973 as IA group known to be the most prevalent races at IRRI in the Philippines, where Tongil has been highly susceptible. This IA group was also pathogenic to Tongil in greenhouse experiments. The race group causing moderately susceptible to Tongil should be new in Korea, since no occurrences of the race IA have ever been reported previously from the areas where japonica types of rice are cultivated. Because some races of P. oryzae present in Korea are pathogenic to Tongil and because these races may become prevalent in the fields, the reaction of Tongil must be continuously monitored.
This study was designed to obtain the mean values of thirteen soil physico-chemical properties for different paddy soil management groups, and to serve the basic information for improving the soil using amendments. Computerized data on the results of detailed soil survey were used in this study. The clay contents in the B horizon of paddy soil management groups were 28.3% in the well adapted type, 11.8% in the sandy textured type, and 26.8% in the newly reclaimed type. Soil pH of B horizon in the paddy soil management groups except poorly drained type and acid sulfate type were higher than those of A horizon. In the river side paddy soils of well adapted type, the clay contents of A and B horizons were 16.8%, 23.1%, respectively, and soil organic matter contents of those horizons were 42g/kg, 18g/kg, respectively. And also available phosphate content of well adapted type was higher than the other types. Frequency of distribution of soil organic matter content levels in the B horizons of sandy textured type and newly reclaimed type were higher in the organic matter content range of below 10g/kg. And those of well adapted, poorly drained, and saline type were higher in the organic matter content range of 10~20g/kg. Correlation coefficients between 13 variables in the B horizon of well adapted type were all highly significant at 1% or 5% level, respectively.
Disposal methods of managing carcass in Korea livestock production systems include burying, digesting, rendering, carcass dumping to manure pile, dead animal disposer and mini-incinerator. Burying was usually the most practical method of carcass disposal in our livestock farms. Burying, carcass dumping to manure pile, dead animal disposer and mini-incinerator may have environmental regulatory and economic liabilities when used as a means of carcass disposal. In many cases in this survey, these disposal methods offer a poor choice for the producer due to individual site conditions, geology, cost, air emissions, rendering plants. A survey questionnaire that addressed the issues to livestock producers was prepared. The questionnaire addressed two main topics as follows: 1) types of livestock and generation amounts of carcass 2) Number of breeding animals and disposal methods of livestock mortality. A total of 36 livestock producers were interviewed. The results of obtained in this survey were summarized as follows: The number of breeding poultry, swine, beef cow and dairy cow was 251,000, 2,600, 142 and 92 heads per year and the generation amounts of annually carcass was 0.46, 15.32, 0.36, 1.36 tons per year of each poultry, swine, beef cow and dairy cow farms, respectively. The disposal methods of carcass were burying (42%), carcass dumping to manure pile (36%), rendering (8%), incineration (6%), digesting (6%), carcass disposer (2%), respectively. These results can be used as basic information to establish the standard of carcass composting facility.
To understand host-defence mechanism of clam(Ruditapes philippinarum) hemocyte against foreign materials, classification and their seasonal change in the number were performed. clams collected from a farm in Julpo Bay, Gochang, Chollabuk-Do were used in this experiment. Lots of hemocytes were found between the muscle fibers and connetive tissue of posterior adductor muscle. Hemocytes of R. philippinarum were classified into granulocytes and agranulocytes. Granulocytes were composed of three types, basophilic granulocyte, acidophilic granulocyte and fibrocyte in accordance with the staining affinities of their cytoplasmic granules. Fibrocyte has filopodia and vesicle in endoplasm and bigger than other granulocytes in size. Agranulocytes were less in the number and smaller in the size compared to those of granulocytes. Hyalinocytes had no granule in their cytoplasm. The nucleus located in the center of the cell was oval or spherical shaped. In electron microscopic observation, granulocytes and hyalinocytes contained electron-dense vesicles and some small lucid vesicles in their cytoplasm, respectively. Granulocytes phagocytosed more zymosan particles than hyalinocytes. Acidophilic granulocytes showed higher phagocytic ratio than basophilic granulocytes. Total hemocyte numbers showed the highest at April to August and the lowest at October to December. In the composition of each hemocyte, basophilic granulocytes were always more than acidophilic granulocytes and hyalinocytes.
1. In the mulberry groves for both rearing of spring and fall, the rate of germination was 74.3 percent and the rate of the unfinished bud was 25.7 percent. 2. When the summer pruning after spring rearing was not worked, new sprouted leaves from the axillary buds of an age old branches was 74.88 percent of germination and the rest from unfinished bud during the spring showed 28.12 percent. 3. In the furrows running from north to south, new buds facing the due north were the worst but towards south-east and north-east were the best in their sprouts. 4. One year-old branches which had been raised without the summer pruning in the bush types of Morus Lhou (ser) Koidz offered a habitat where Apriona Rugico1lis Cherr could live. 5. On the grove of Morus Lhou Koidz cultivated at lower zone under a higher humidity and the shortage of sun light the appearance of Anomoneura Mori Schw was likely to happen very much. 6. One year old branches without the summer pruning produced more leaves owing to a long period of growth than branches with the summer pruning in the fall crops. 7. It is necessary to reconsider a certain distance of planting because of causing, when the summer pruning was not made in the bush plantation, a obstacle of fertilizer operation or the forth coming year's management. 8. It can not be thought the ideal method for us to raise mulberry tree such as Morus Lhou Koidz under natural form and at a short distance of planting (one another). 9. Among the species, Morus alba L. had a great deal of leaves and of the rate of germination per unit length of branch.
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