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Prediction of Dietary Protein-Energy Balance by Milk Urea Nitrogen and Protein Contents in Dairy Cow (젖소의 우유 중 단백질과 요소태질소 측정에 의한 사료의 에너지와 단백질 균형 상태 예측)

  • Moon, J.S.;Joo, Y.S.;Kang, H.M.;Jang, G.C.;Kim, J.M.;Lee, B.K.;Park, Y.H.;Son, C.H.
    • Journal of Animal Science and Technology
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    • v.44 no.5
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    • pp.573-584
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    • 2002
  • Milk urea nitrogen (MUN) and Milk protein (MP) are being used as indicators of the protein-energy balance and for actual farm feeding practices. The purpose of this study was to investigate the MUN and MP concentrations of individual cows and bulk tank milk to evaluate the dietary protein-energy balance from lactating Holstein cows. Mean MUN and MP concentrations in the milk samples obtained from 132,636 cows of 4,856 herd during Jan. 1999 to Dec. 2001 were 16.2 5.2mg/dl and 3.30 0.35%, respectively. The highest values were found during summer and lowest valued during winter in MUN. But, the average contents of MP were the highest during winter and the lowest during summer. In order to evaluate protein-energy balance for feeding, we set the level of recommended MP range of 2.90${\sim}$3.29% in early lactation considering a negative energy balance. The recommended level of MP in mid-lactation and late lactation were set as 3.10${\sim}$3.49%, and 3.30${\sim}$3.69%, respectively. Recommended MUN range of 12${\sim}$18 mg/dl was determined through the whole lactation period. Individual cows milk were analyzed by the 9 types based on this levels of MP and MUN in this study. Among the total cows investigated, 26.8%, 25.8%, and 22.2% have shown the recommended criteria of MP and MUN values, respectively. Also, of total herds surveyed, 11.6% had MUN values lower than 12.0 mg/dl and 32.9% had values higher than 18.0 mg/dl and 44.5% of total herd have not met with the recommended criteria of MP values in bulk tank milk. In case of MP, out of the total herd surveyed, 26.0% had MP values lower than 3.10% and 24.0% had values higher than 3.30% and 50.0% had MP values outside the recommended interval (3.1${\sim}$3.3%). This study has indicates that many dairy farms are under improper feeding management practice of the dietary protein-energy balance.

Effect of Red clay(Hwangto) on Growth Performance and Carcass Characteristics in Growing-Fattening Hanwoo Steers (육성비육 거세한우에 대한 황토 급여가 성장 및 도체특성에 미치는 효과)

  • Kang, S.W.;Kim, J.S.;Cho, W.M.;Chung, H.Y.;Ki, K.S.;Choe, S.B.
    • Journal of Animal Science and Technology
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    • v.44 no.3
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    • pp.315-326
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    • 2002
  • This study was conducted to examine the effects of Red clay(Hwangto) as feed additives with 26 Hanwoo steers at two regions for 540 days from six to 24 months of age on feed efficiency, meat quantity and meat quality. Feeding trial 1 was conducted with two treatment groups by five heads/treatment which were T1(Control) and T2(Control+ad libitum Hwangto) at National Livestock Research Institute. Feeding trial 2 was conducted with four treatment groups by four heads/treatment which were T1(Control), T2(Control+2% Hwangto), T3(Control+5% Hwangto) and T4(Control+ad libitum Hwangto) at a private Hanwoo breeding farm. In feeding trial 1, average daily gains by growing, fattening, finishing and over-all periods were 0.572, 0.866, 0.869 and 0.769kg in T1, and 0.537, 0.904, 0.857 and 0.766kg in T2, respectively. Average daily gain for growing and finishing period was high in T1 but in T2 for fattening period, which were not statistically significant. Also concentrates and TDN intakes per kg gains were not statistically significant by treatments. In carcass characteristic, dressed carcass and red meat percentage were high in T1 but in T2 for retailed cut percentage without significant difference. The yield index and shear force was 69 and 70, 4.4 and 6.1 kg/$cm^2$ in T1 and T2, respectively. In palatability traits, the panel test scores of juiciness, tenderness and flavor were 4.08 and 4.64, 3.42 and 3.96, 4.58 and 4.80 in T1 and T2, respectively. which showed a tendency to improvement in Hwangto feeding groups. In feeding trial 2, the range of average daily gains were 0.656(T6) to 0.772(T3), 0.937(T6) to 1.009(T5), 0.592(T3) to 0.675kg(T6) in growing, fattening and finishing period, respectively. Although it was high in T3, T5 and T6 at growing, fattening and finishing period, respectively, it was not significantly different by treatments. The ranges of concentrates and TDN intakes per kg gains were 8.71 to 9.50(average 9.05) and 7.52 to 7.64kg(average 7.55kg), they all were lower in Hwangto feeding groups than in control about 4.5 to 8.3% and 1.2 to 1.6%, respectively. In carcass characteristics, back-fat thickness, loin eye muscle area and yield index were not difference by treatments, but marbling scores were high in T5 and T6 than in percent. Summarizing above results, it may be concluded that the use of unprocessed Hwangto as feed additives for growing-fattening steers seems to improve meat quality but not red meat quantity and feed efficiency, and may be required to feed ad libitum during the finishing period.

Genetic Analysis for Traits at Birth and Weaning in Mice (Mouse의 생시(生時) 및 이유시(離乳時) 형질(形質)에 대(對)한 유전분석(遺傳分析))

  • Choi, Kwang Soo;Park, Hang Kyun;Hyun, Byung Hwa
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.158-165
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    • 1985
  • The study was conducted to obtain some genetic information for breeding of mice. Average performance, heterosis and genetic variance were estimated with 362 progenies from a full diallel cross of four lines of mice (BALB/c, CBA, C3H and C57BL). The progenies were reared at the Experimental Animal Farm. College of Agriculture, Kyungpook National University from November, 1984 to February, 1985. Data for litter size. sex ratio, body weight and weaning rate were analyzed into heterosis effects, and genetic variance with Hayman's model. The results obtained are summarized as follows : 1. Average performance was 7.54 in litter size, 53.20 % in sex ratio, 1.55 g in birth weight, 10.45 g in weaning weight and 94.13 % in weaning rate. 2. The estimated heterois was 6.97% in litter size, 7.26 % in sex ratio, 6.08% in birth weight, 3.54% in weaning weight and 2.05% in weaning rate, respectively. 3. Additive gene effects were not observed in litter size, sex ratio, birth weight, weaning weight and weaning rate. In litter size and weaning weight, dominance effects were observed, which were shown due to individual crosses in litter size and were shown mean dominance effects of parental line in weaning weight. Mat-Maternal effects appeared in birth weight and weaning weight, and reciprocal effects were observed in weaning weight.

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A Study on the Consumptive use of Irrigated Water in Paddy Rice (벼의 품종별(品種別) 필요수량(必要水量) 관(關)한 실험분석(實驗分析))

  • Suh, Seung Duk;Choi, Won Chil
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.36-43
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    • 1985
  • This study was conducted to find out the consumptive use of irrigated water for calculation of a duty of water in paddy rice. Tall statured Japonica rice variety is Jinju(late). Short statured Tongil varities-Taebaek(early) and Pungsan(medium) were planted on the experimental farm of kyungpook National University in 1983. The results obtained in this study were as follows : 1. The average temperature of the underwater surface was $27.1^{\circ}C$ and the maximum was recorded at beginning of the August. 2. The evapotranspiration was gradually increased after transplanting and showed the peak from booting to heading stage of rice varieties. The average evapotranspiration through the whole growing period was 8. 43mm/day(Jinju) for tall statured Japonica variety, and 7.78mm/day(Taebaek), 7.71mm/day(Pungsan) for short statured Tongil varieties. 3. The ratio of evapotranspiration to pan-evaporation through the whole growing period was 1.71 for Japonica variety and 1.55 for Tongil varieties. 4. K-value in Blaney & Criddle formula was 1.41 for Japonica variety and 1.30 for Tongil varieties. Coefficient consumptive use of water (Kc-value) was 1.40 for Japonica variety and 1.27 for Tongil varieties. 5. It appeared that the water consumption in the 6, 8 cm pot is more than that of 2, 4 cm pot but the number of panicle of the 6, 8 cm pot is somewhat less than that of 2, 4 cm pot. The deeper water level is the less tiller became. It is consindered that 2 cm pot is more profitable than the others.

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Effect of ethanol extract of Lotus Rhizome and node of Lotus Rhizome (연근과 우절 에탄올 추출물의 향장효능 검증)

  • Jang, Young-Ah;Park, So-Hyun;Kim, Bo-Ae;Park, Jong-Yi;Jeoung, Young-Ok;Lee, Jin-Tae
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.657-665
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    • 2017
  • This study is for checking the possibility of Lotus Rhizome and node of Lotus Rhizome as cosmetic materials. For this we carried out biological active evaluation about anti-oxidant, anti-inflammatory and anti-wrinkle by using ethanol extract of Lotus Rhizome and node of Lotus Rhizome. We extracted Lotus Rhizome and node of Lotus Rhizome with 95% ethanol and then in order to evaluate anti-oxidant activity we treated samples by concentrations (100, 500, 1000) ${\mu}g/mL$ and carried out 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and The activity of 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging. Also, in order to evaluate effect of anti-wrinkle we carried out evaluation of Elastase inhibitory activity. To evaluate effect of anti-inflammatory we evaluated toxicity of samples through MTT assay with a macrophage (Raw 264.7 cells) and measured nitric oxide production inhibitory activity. As a result, DPPH radical scavenging activity of Lotus Rhizome and node of Lotus Rhizome at $1000{\mu}g/mL$ was 66.7% and 99.5%, respectively and ABTS + radical scavenging activity was 51.2% and 98.3% at the same concentration, respectively. Elastase inhibitory activity results showed that the nodes of the Lotus Rhizom extract excellent anti-wrinkle efficacy than Lotus Rhizom. Node of Lotus Rhizome showed higher anti-wrinkle activity than the positive the control group BHT at $1000{\mu}g/mL$ concentration. According to the result of nitric oxide production inhibitory activity, Lotus Rhizom showed 55.8% effect and nodes of the Lotus Rhizom showed 66.6% effect respectively. This showed that effect of anti-inflammatory was greater in nodes of the Lotus Rhizom extracts. As a result it suggests that Lotus Rhizome and node of Lotus Rhizome extracts can be used as natural substance of cosmetics which are safe in antioxidant, anti-inflammatory, and anti-wrinkle.

EFFICIENCY OF ENERGY TRANSFER BY A POPULATION OF THE FARMED PACIFIC OYSTER, CRASSOSTREA GIGAS IN GEOJE-HANSAN BAY (거제${\cdot}$한산만 양식굴 Crassostrea gigas의 에너지 전환 효율)

  • KIM Yong Sool
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.179-183
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    • 1980
  • The efficiency of energy transfer by a population of the farmed pacific oyster, Crassostrea gigas was studied during culture period of 10 months July 1979-April 1980, in Geoje-Hansan Bay near Chungmu City. Energy use by the farmed oyster population was calculated from estimates of half-a-month unit age specific natural mortality rate and data on growth, gonad output, shell organic matter production and respiration. Total mortality during the culture period was estimated approximate $36\%$ from data on survivor individual number per cluster. Growth may be dual consisted of a curved line during the first half culture period (July-November) and a linear line in the later half period (December-April). The first half growth was approximated by the von Bertalanffy growth model; shell height, $SH=6.33\;(1-e^{0.2421(t+0.54)})$, where t is age in half-a-month unit. In the later half growth period shell height was related to t by SH=4.44+0.14t. Dry meat weight (DW) was related to shell height by log $DW=-2.2907+2.589{\cdot}log\;SH,\;(2, and/or log $DW=-5.8153+7.208{\cdot}log\;SH,\;(5. Size specific gonad output (G) as calculated by condition index of before and after the spawning season, was related to shell height by $G=0.0145+(3.95\times10^{-3}{\times}SH^{2.9861})$. Shell organic matter production (SO) was related to shell height by log $SO=-3.1884+2.527{\cdot}1og\;SH$. Size and temperature specific respiration rate (R) as determined in biotron system with controlled temperature, was related to dry meat weight and temperature (T) by log $R=(0.386T-0.5381)+(0.6409-0.0083T){\cdot}log\;DW$. The energy used in metabolism was calculated from size, temperature specific respiration and data on body composition. The calorie contents of oyster meat were estimated by bomb calorimetry based on nitrogen correction. The assimilation efficiency of the oyster estimated directly by a insoluble crude silicate method gave $55.5\%$. From the information presently available by other workers, the assimilation efficiency ranges between $40\%\;and\;70\%$. Twenty seven point four percent of the filtered food material expressed by energy value for oyster population was estimated to have been rejected as pseudofaeces : $17.2\%$ was passed as faeces; $35.04\%$ was respired and lost as heat; $0.38\%$ was bounded up in shell organics; $2.74\%$ was released as gonad output, $2.06\%$ was fell as meat reducing by mortality. The remaining $15.28\%$ was used as meat production. The net efficiency of energy transfer from assimilation to meat production (yield/assimilation) of a farm population of the oyster was estimated to be $28\%$ during culture period July 1979-April 1980. The gross efficiency of energy transfer from ingestion to meat production (yield/food filtered) is probably between $11\%\;and\;20\%$.

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Assessment of Pre-Harvest Environmental Factors in Domestic Production of Organic Lettuce (국내 유기상추의 생산환경 조사분석)

  • Namgung, Min;Kim, Beom Seok;Heo, Seong Jin;Choi, Yong Beom;Hur, Jang Hyun;Park, Duck Hwan
    • The Korean Journal of Pesticide Science
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    • v.18 no.2
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    • pp.88-94
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    • 2014
  • Among pre-harvest environmental factors, increasing attention has been paid to the effects of chemical and microbiological factors on fresh produce. The occurrence and prevalence of these factors have been usually studied with regard to the final products at the post-harvesting stage and/or when they are sold in the market. However, the origin and routes of transmission of both factors remain to be clarified. In the present study, we examined the contamination levels of food-borne pathogens and chemical factors such as pesticide residues and heavy metals in 83 and 43 samples, respectively, including various soil, water, and fertilizer samples, as well as post-harvested and processed samples. Among the organic farming samples, only one pesticide, dimethomorph, was detected in the soil sample, however no pesticides were observed from any other samples in organic farming system. Thus, it was thought that might be contaminated from conventional farm land in the vicinity. Whereas many pesticide residues were detected in conventional farming systems such as soil, fertilizer, water, and fresh produce as expected. Furthermore, heavy metals detected from all tested samples did not shown contamination levels higher than the standard limit. We comparatively assessed the levels of contamination by food-borne pathogens on the samples from organic and conventional farming systems, and found aerobic bacteria at approximately 7 log CFU/g, with no significant differences observed between the two systems. Coliforms were present at lower levels than aerobic bacteria. No human pathogens were present among the coliforms detected, indicating that these bacteria are saprophytes without the ability to cause food-borne illnesses. In contrast, among the high-risk food-borne pathogens, only sporadic cells of Bacillus cereus were found on samples of organic farming system. These data extend previous findings that the most prevalent food-borne pathogen is B. cereus and demonstrate that it spreads to whole living plants via soil.

Study on shipment time of low-temperature stored apple (저온저장 사과의 출하시기에 대한 실태조사 연구)

  • Yu, Chang-Hwan;Kim, Yun-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.554-564
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    • 2017
  • The purpose of this study is to assist farmers in determining the optimal time for the shipment of stored apples by investigating the market trends, storage status and release dates of low-temperature stored apples. We surveyed 300 apple cultivation farms in Daegu, Gyeongbuk Province, which is the main producer of apples, and examined the cultivars, cultivation areas, storage conditions, and shipment status of apples. The main results are as follows: the proportion of apples cultivatedat different ripening times was surveyed. According to the results, the proportions of early, middle and late varieties were 78.3%, 63.7% and 96.0%, respectively. Also, 48.7% of the farm households surveyed had cold storage facilities. The average storage cost per apple box(15kg) was 978.3 won for self-storage and 1,771.8 won for consignment storage. For the six(6) months between November and April, the proportion of shipped apples was 91.6% of the total stored apples. The (average total?)annual apple shipment, including apples stored in general storage warehouses, was 744.4 boxes. The (average total?) annual shipment of cold storage apples was 616 boxes. The stored apples were mainly shipped to 'wholesale markets', which have the highest sales share, followed by 'production site collectors' and then 'supermarkets'. The most common shipping method of the apples was by general trucks, followed by low-temperature trucks, and finally by delivery services. The analysis of the factors influencing the decision to release apples by period showed that it was affected by the storage cost, loss rate, and customary shipping in the off-season(from May to August). On the other hand, in the general release season(from November to April), the statistically significant decision factors for the release of apples were the future expected price, storage cost and decision of the leading farmer groups. For farmers with a high share of general shipment, the deciding factors for the release of apples were the future expected price, storage cost, high income expectation, and decision by leading farmers.

Effects of floor type and hanging type environmental enrichment on the behavior of growing pigs (바닥형과 현수형 환경 보조물이 육성돈의 행동에 미치는 영향)

  • Kim, Doo-Wan;Kim, Young-Hwa;Min, Ye-Jin;Yu, Dong-Jo;Jeong, Yong-Dae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.11
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    • pp.282-289
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    • 2017
  • Animal Welfare is spreading throughout the world, but remains weak in Korean swine farms. Therefore, the aim of this study was to identify the effect of floor type and hanging type environmental enrichment on the behavior of growing pigs under the traditional feeding environment. A total of 45 crossbred pigs (Yorkshire${\times}$Landrace${\times}$Duroc; average weight, $33.35{\pm}5.5kg$) were assigned into three treatments consisting of control, hanging type(T1) and floor type(T2) with three replicates in semi-slurry pen. The hanging enrichment was suspended at shoulder height of the pigs, and the floor enrichment was fixed in the center of the pens. Growth and cortisol were estimated at the end of the experimental period. Behaviour patterns were analyzed on the first and eighth days after starting the experiment. Growth was not differed among control and treatments. However, cortisol was decreased in T2 compared to control(25.28 vs. 46.75 ng/mL; p<0.05). On the first day, movement and aggression were lower in both treatments than in control(p<0.01). On the eighth day, time and frequency of playing action were increased in T2 compared to T1(p<0.01) and both treatment groups showed more active behaviour than control(p<0.01). These results suggested that the enrichment may meet the natural action requirement of pigs. Therefore, our data can be utilized as basic information for welfare with environmental enrichments in farm animals.

Characterization of Protease Produced by Elizabethkingia meningoseptica CS2-1 and Optimization of Cultural Conditions for Amino Acid Production (닭 우모 분해세균 Elizabethkingia meningoseptica CS2-1이 생산하는 단백질분해효소의 특성 및 아미노산 생산을 위한 배양조건)

  • Kim, Se-Jong;Cho, Chun-Hwi;Whang, Kyung-Sook
    • Journal of Applied Biological Chemistry
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    • v.54 no.2
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    • pp.135-142
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    • 2011
  • A feather-degrading bacterium Elizabethkingia meningoseptica CS2-1 was isolated from compost in a chicken farm. Cultured on a basal medium containing 2% chicken feather, the bacterium showed 729.7 ${\mu}mol/mL$ of amino acid. Optimal culture conditions for feather degradation by E. meningoseptica CS2-1 were $25^{\circ}C$, pH 7.5, and 180 rpm. The optimal pH and temperature for protease activity were 8.0 and $40^{\circ}C$, respectively. The composition of an optimal medium for amino acid production was 0.05% NH4Cl, 0.05% NaCl, 0.03% $K_2HPO_4$, 0.03% $KH_2PO_4$, 0.01% $MgCl_2{\cdot}6H_2O$, 0.1% urea, and 2% chicken feather. Characteristics of amino acids extracted from the optimal medium under the optimal culture conditions of E. meningoseptica CS2-1 were analyzed. The total amino acid content of strain CS2-1 was 1063 ${\mu}mol/mL$, which was 46% higher compared to the basal condition (729.7 ${\mu}mol/mL$). The essential amino acid content in the total amino acid was 315.9 ${\mu}mol/mL$, which was 44% higher than that of the basal condition. Major amino acids were proline (14%), aspartic acid (12%), glutamic acid (11%), serine (10%), alanine (10%), glycine (9%), and tyrosine (7%) by strain CS2-1. These results suggest that strain CS2-1 can be used as a potential microbial resource for the production of amino acid using chicken feathers.