• 제목/요약/키워드: failure line

검색결과 839건 처리시간 0.027초

TLR-1, TLR-2, and TLR-6 MYD88-dependent signaling pathway: A potential factor in the interaction of high-DNA fragmentation human sperm with fallopian tube epithelial cells

  • Zahra Zandieh;Azam Govahi;Azin Aghamajidi;Ehsan Raoufi;Fatemehsadat Amjadi;Samaneh Aghajanpour;Masoomeh Golestan;Reza Aflatoonian
    • Clinical and Experimental Reproductive Medicine
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    • 제50권1호
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    • pp.44-52
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    • 2023
  • Objective: The DNA integrity of spermatozoa that attach to fallopian tube (FT) cells is higher than spermatozoa that do not attach. FT epithelial cells can distinguish normal and abnormal sperm chromatin. This study investigated the effects of sperm with a high-DNA fragmentation index (DFI) from men with unexplained repeated implantation failure (RIF) on the Toll-like receptor (TLR) signaling pathway in human FT cells in vitro. Methods: Ten men with a RIF history and high-DFI and 10 healthy donors with low-DFI comprised the high-DFI (>30%) and control (<30%) groups, respectively. After fresh semen preparation, sperm were co-cultured with a human FT epithelial cell line (OE-E6/E7) for 24 hours. RNA was extracted from the cell line and the human innate and adaptive immune responses were tested using an RT2 profiler polymerase chain reaction (PCR) array. Results: The PCR array data showed significantly higher TLR-1, TLR-2, TLR-3, TLR-6, interleukin 1α (IL-1α), IL-1β, IL-6, IL-12, interferon α (IFN-α), IFN-β, tumor necrosis factor α (TNF-α), CXCL8, GM-CSF, G-CSF, CD14, ELK1, IRAK1, IRAK2, IRAK4, IRF1, IRF3, LY96, MAP2K3, MAP2K4, MAP3K7, MAP4K4, MAPK8, MAPK8IP3, MYD88, NFKB1, NFKB2, REL, TIRAP, and TRAF6 expression in the high-DFI group than in the control group. These factors are all involved in the TLR-MyD88 signaling pathway. Conclusion: The MyD88-dependent pathway through TLR-1, TLR-2, and TLR-6 activation may be one of the main inflammatory pathways activated by high-DFI sperm from men with RIF. Following activation of this pathway, epithelial cells produce inflammatory cytokines, resulting in neutrophil infiltration, activation, phagocytosis, neutrophil extracellular trap formation, and apoptosis.

An Experimental Study on the Ultimate Strength and Deformation Capacity of Composite Beams with Eccentric Web Openings (편심유공합성보의 종국내력 및 변형능력에 관한 실험적 연구)

  • Choi, San Ho;Seo, Seong Yeon
    • Journal of Korean Society of Steel Construction
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    • 제12권5호통권48호
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    • pp.595-604
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    • 2000
  • Web openings of large beams provide space for wiring, piping, and duct work to provide for proper drainage, pipes and duct must be slightly sloped with the attendant result that all web openings can not be centered on the centroidal axes of the beams. Test specimens are made for opening-depth to beam-depth ratio of 0.5 and for eccentricities of the opening center line of 10% from middepth of the beam because of the proximity of the opening edge to the flange. In this paper, available test results and theories relating to the strength of composite beams having eccentric rectangular openings are surveyed and experiments were carried out to examine the structural behaviors. In all the tests in this paper good agreement is demonstrated with maximum loads measured in tests, and observed failure modes Furthermore, compared with analytical values and experimental values of interaction diagram between moment and shear capacity were safed as it is scattered with outer part of the analytical values.

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The Earth Pressure on the Effect of Surcharge Load at the Narrowly Backfilled Soil (좁은 공간 되메움 지반에서의 상재하 영향에 의한 토압)

  • 문창열;이종규
    • Geotechnical Engineering
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    • 제13권6호
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    • pp.165-180
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    • 1997
  • The structure such as underground external walls of buildings, conduit and box culvert supports the surcharge loads (point, strip and line loads) . The vertical and horizontal stresses in a soil mass depend on the backfill width and wall friction, etc. The investigations described in this paper is designed to identify the magnitude and the distributions of the lateral and vertical pressure which is occurred by the narrowly backfilled soil in an open cut by the surcharge loads. For these purposes, model tests were performed for various width of backfill in a model test box by considering the wall friction using carbon rods. The results of test were compared with the theories of Weissenbach and VS Army Code and also with the results of the numerical analysis using finite difference method which introduces Mohr-Coulomb failure hypothesis.

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Vulnerability Assessment Procedure for the Warship Including the Effect of Shotline and Penetration of Fragments (탄두의 관통 효과를 고려한 함정 취약성 평가 절차에 관한 기본 연구)

  • Kim, Kwang-Sik;Lee, Jang-Hyun
    • Journal of the Society of Naval Architects of Korea
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    • 제49권3호
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    • pp.254-263
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    • 2012
  • The survivability of warship is assessed by susceptibility, vulnerability and recoverability. Essentially, a vulnerability assessment is a measure of the effectiveness of a warship to resist hostile weapon effects. Considering the shot line and its penetration effect on the warship, present study introduces the procedural aspects of vulnerability assessments of warship. Present study also considers the prediction of penetration damage to a target caused by the impact of projectiles. It reflects the interaction between the weapon and the target from a perspective of vulnerable area method and COVART model. The shotline and tracing calculation have been directly integrated into the vulnerability assessment method based on the penetration equation empirically obtained. A simplified geometric description of the desired target and specification of a threat type is incorporated with the penetration effect. This study describes how to expand the vulnerable area assessment method to the penetration effect. Finally, an example shows that the proposed method can provide the vulnerability parameters of the warship or its component under threat being hit through tracing the shotline path thereby enabling the vulnerability calculation. In addition, the proposed procedure enabling the calculation of the component's multi-hit vulnerability introduces a propulsion system in dealing with redundant Non-overlapping components.

An Analysis on the Deployment Methods for Smart Monitoring Systems (스마트 모니터링 시스템의 배치 방식 분석)

  • Heo, No-Jeong
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • 제10권6호
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    • pp.55-62
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    • 2010
  • Monitoring systems are able to report certain events at region of interest(ROI) and to take an appropriate action. From industrial product line full of robots to fire detection, intrusion detection, smart grid application, environmental pollution alarm system, monitoring system has widely used in diverse industry sector. Recently, due to advance of wireless communication technology and availability of low cost sensors, intelligent and/or smart monitoring systems such as sensor networks has been developed. Several deployment methods are introduced to meet various monitoring needs and deployment performance criteria are also summarized to be used to identify weak point and be useful at designing monitoring systems. Both efficiency during deployment and usefulness after the deployment should be assessed. Efficiency factors during deployment are elapsed time, energy required, deployment cost, safety, sensor node failure rate, scalability. Usefulness factors after deployment are ROI coverage, connectivity, uniformity, target density similarity, energy consumption rate per unit time and so on.

Use of IMS-RT-PCR for the Rapid Isolation and Detection of Hepatitis A Virus from the Swine Feces (IMS-RT-PCR을 활용한 육성돈 분변으로부터 간염 A형 바이러스의 신속순수분리 및 검출법 연구)

  • Lee, Hee-Min;Kim, Duwoon
    • Korean Journal of Food Science and Technology
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    • 제44권5호
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    • pp.638-642
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    • 2012
  • Human pathogenic viruses such as hepatitis A and E virus (HAV and HEV), which lead to acute liver failure and death, are foodborne pathogens associated with the consumption of virus-contaminated meats, filter-feeding bivalves, fruits, and salads. Two of the three swine farms examined in this study had HAV and HEV positive stool samples in a nested RT-PCR assay. The use of the immunomagnetic separation (IMS) facilitated the separation of HAV through interactions between the ligand on the virion surface and the antibody from the swine feces containing both HAV and HEV. The nested RT-PCR analysis was performed for the detection of HAV obtained from hepatocarcinoma cell line (PLC/PRF/5) contaminated with eluent fraction of IMS. This indicated that IMS has the potential to simultaneously isolate and concentrate target viruses by changing antibodies linked on the magnetic beads.

Operation and Command of Virtual Router Redundancy Protocol in Open N2OS (Open N2OS를 활용한 가상 라우터 이중화 프로토콜의 기능 동작과 명령어)

  • Lee, ChangSik;Ryu, HoYong;Park, Jaehyung
    • Journal of Digital Contents Society
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    • 제19권4호
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    • pp.693-700
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    • 2018
  • Virtual router redundancy protocol (VRRP) was designed as a solution to support fast fail-over in case of network failure. There exists virtual router which acts as default gateway in LAN, and the virtual router is dynamically elected between master and backup router. Through this protocol, end-hosts can be provided seamless network service. However, it needs expensive license fees and maintenance costs to adopt current commercial network operating systems. Furthermore, they are commonly enterprise proprietary software and inherently closed source. In order to tackle these problem, Open N2OS which is open source based open network software platform was developed. It has no dependency on hardware equipment, and provides high availability, scalability, various networking functions. In this paper, we handle VRRP operation and mechanism with related command line interface (CLI).

A 12-kV HBM ESD Power Clamp Circuit with Latchup-Free Design for High-Voltage Integrated Circuits (고전압 집적회로를 위한 래치업-프리 구조의 HBM 12kV ESD 보호회로)

  • Park, Jae-Young;Song, Jong-Kyu;Jang, Chang-Soo;Kim, San-Hong;Jung, Won-Young;Kim, Taek-Soo
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • 제46권1호
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    • pp.1-6
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    • 2009
  • The holding voltage of high-voltage devices under the snapback breakdown condition has been known to be much smaller than the operating voltage. Such characteristics cause high-voltage ICs to be susceptible to the transient latch-up failure in the practical system applications, especially when these devices are used as the ESD(ElectroStatic Discharge) power clamp circuit. A new latchup-free design of the ESD power clamp circuit with stacked-bipolar devices is proposed and successfully verified in a $0.35{\mu}m$ 3.3V/60V BCD(Bipolar-CMOS-DMOS) process to achieve the desired ESD level. The total holding voltage of the stacked-bipolar devices in the snapback breakdown condition can be larger than the operating voltage. Proposed power clamp operates safely because of the high holding voltage. From the measurement on the devices fabricated using a $0.35{\mu}m$ BCD Process, it was observed that the proposed ESD power clamp can provide 800% higher ESD robustness per silicon area as compared to the conventional clamps with a high-voltage diode.

Ultra-Power-Saving 2 Ports PLC Wall Switch Development (초절전형 PLC 2구 스위치 개발)

  • Han, Jae-Yong;Lee, Sun-Heum
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • 제44권11호
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    • pp.51-55
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    • 2007
  • Generally, PLC (Power Line Communication) based home automation devices such as wall switch, walt socket, gas controller, etc, must maintain wake-up status at all time to control other electronic devices and monitor their on/off status whether they are in service or not. In order to reduce the unnecessary energy consumption during the standby mode, the new power-saving PLC 2 ports wall switch has been developed, separating PLC communication part and controller part and introducing sleep mode. In addition, to expand life cycle of PLC product and to reduce the rate of product failure in active mode, the instant controlling method in controlling process is adopted instead of the maintenance controlling method. In comparison to the earlier model, the new 2 ports PLC wall switch has reduced power by 0.95[W] less in standby mode and 3.2[W] less in active mode than the previous one.

Nonlinear Analysis of RC Members Using Truss Model (트러스 모델을 이용한 철근콘크리트 부재의 비선형해석)

  • Eom, Tae-Sung;Park, Hong-Gun
    • Journal of the Korea Concrete Institute
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    • 제18권2호
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    • pp.177-188
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    • 2006
  • Conventional nonlinear finite element analysis requires complicated modeling and analytical technique. Furthermore, it is difficult to interpret the analytical results presented as the stress-strain relationship. In the present study, a design-oriented analytical method using the truss model was developed. A reinforced concrete member to be analyzed was idealized by longitudinal, transverse, and diagonal line elements. Basically, each element was modeled as a composite element of concrete and re-bars. Simplified cyclic models for the concrete and re-bar elements were developed. RC beams and walls with various reinforcement details were analyzed by the proposed method. The inelastic strength, energy dissipation capacity, deformability, and failure mode predicted by the proposed method were compared with those of existing experiments. The results showed that the proposed model accurately predicted the strength and energy dissipation capacities, and to predict deformability of the members, the compression-softening model used for the concrete strut element must be improved.