• 한국미생물·생명공학회지
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• 제24권5호
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• pp.579-584
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• 1996

The molecular weight of the purified nucleoside oxidase estimated by gel filtration column chromatography was 480,000 and the enzyme protein was composed of four nonidentical subunits (81,000, 69,000, 32,000 and 16,000). On the basis of the visible absorption spectra and the enzymatic determination of the purified enzyme, the enzyme was supposed as a hemoprotein and also a flavoprotein containing 3 moles of FAD per I mole of enzyme. The isoelectric point of the enzyme was pH 5.1. Addition of metal salts such as 1 mM SnCl$_{2}$ and PbCl$_{2}$ into an enzyme reaction solution inhibited the enzyme activity by 94 and 90%, respectively. The enzyme activity was also lost significantly by hemoenzyme inhibitors such as NaCN and NaN$_{3}$ and flavoenzyme inhibitor, acriflavine and quinacrine. The maximal nucleoside oxidase activity was observed at pH 7.0 and 55$\circ$C. The nucleoside oxidase was relatively stable in the range of pH 5.5-9.0 and below 55$\circ$C.

• Journal of Life Science
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• 제9권1호
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• pp.64-68
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• 1999

The properties of purified intracellular adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) of Nocardioides sp. J-326TK isolated from soil have been studied. The enzyme deaminated adenosine and 2`-deoxyadenosine and the respective {TEX}$K_{M}${/TEX} values were 4.0×{TEX}$10^{-4}${/TEX} M and 5.0× {TEX}$10^{-4}${/TEX} M, but the enzyme was not active on 8-bromoadenosine, 6-methylaminopurine riboside, ATP, ADP, 2`-AMP, 3`-AMP, 5`-AMP, dAMP, cAMP, NAD, FAD, NADP and adenine. The enzyme activity was strongly inhibited by the addition of {TEX}$Hg^{2+}${/TEX} and {TEX}$Ag^{+}${/TEX}, {TEX}$Cu^{2+}${/TEX}, {TEX}$Co^{2+}${/TEX} and {TEX}$Mn^{2+}${/TEX} also inhibited the activity but much less extent. The effect of alkyl reagents, metal chelating reagents and certain other compounds on the enzyme activity were also examined. No reagent activated the enzyme. On the contrary, the enzyme reaction was slightly inhibited by o-phenanthroline and 6-benzyladenosine.

• 한국진공학회지
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• 제7권s1호
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• pp.7-14
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• 1998

Details are given of an study of the characteristics of field-induced electron emission from hydrogen-free high $sp^3$ content(>90%) amorphous diamond (a-D) film deposited on heavily doped ($\rho$<0.01 $\Omega\cdot\textrm{cm}$) n-type monocrystalline Si(111) substrate. It is demonstrated that a-D film has excellent electron field emission properties. Emission current can reach 0.9 $\mu$A at applied field as low as 1 V/$\mu\textrm{m}$, and emission current density can be obtained about several mA/$\textrm{cm}^2$. The emission current is stable when the beginning current is at 50 $\mu$A within 72 hours. Uniform fluorescence display of electron emission from whole face of the a-D film under the electric field of 10~20 V/$\mu\textrm{m}$ was also observed. It can be considered that the contribution of excellent electron emission property results from its smooth, uniform, amorphous surface and high $sp^3$ content of the a-D films.

• 한국항공우주학회지
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• 제47권12호
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• pp.831-840
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• 2019

플러시 대기자료 측정장치는 비행체 표면에서 측정되는 압력 데이터를 이용하여 대기자료를 예측한다. FADS는 돌출된 프로브가 없으므로 고성능 항공기, 스텔스 비행체 및 극초음속 비행체에 적합하다. 본 논문에서는 구-원추 형상을 갖는 비행체에 대해서 아음속부터 초음속 비행까지 대기자료를 예측할 수 있는 FADS의 교정 절차와 계산 알고리즘을 제시한다. 표면 압력 데이터 측정을 위해 노즈부 표면에 5개 플러시 압력공들을 마련하였다. 유동각 예측과 압력 관련 변수의 예측을 분리하는 개념이며, 아음속 유동의 포텐셜 유동해와 극초음속 유동의 수정 뉴톤식을 결합한 압력모델을 사용한다. 교정 압력 데이터는 Euler 방정식을 푸는 전산유체역학 코드를 만들어서 마흐수 0.5 ~ 3.0의 범위에서 구축하였다. 비행 마흐 수 0.6~3.0, 받음각과 옆미끄럼각은 각각 -10° ~ +10°의 범위에서 여러 비행조건에 대해서 테스트를 수행하였다. 예측된 대기자료는 받음각, 옆미끄럼각, 마흐수, 자유류 정압이며 참고 데이터와 비교하여 정확도를 분석하였다.

• 종양간호연구
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• 제3권2호
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• pp.155-163
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• 2003

This study was designed to assess the level of family function between cancer patient's families and normal families, and to seek better quality family-Mcentered nursing care. The subjects for this study were 95 ca patient's families who were selected from th university hospitals and 95 normal families who selected from 1 university hospital. The instrument used for this study was McMaster Family Assessment Device (FAD) developed by Epstein, Baldwin and Bishop (1983). The scale consisted of 53 items covering 7 areas: 'problem solving', 'communication', 'roles', 'affective responsiveness', 'affective involvement', 'behavior control', and 'general functioning'. As for data analyses, descriptive statistics, ${\chi}^2-test$, t-test, ANOVA were adopted using SAS program. The result of this study was as follows: There there was no significant difference between cancer patient's family function and normal family function. Also there was no significant difference on cancer patient's family function with regard to family characteristics, disease's characteristics, and clinical stage of cancer.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.73-74
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• 2003

Familial form of Alzheimer's disease (FAD) is caused by mutations in presenilin-1 and presenilin-2 (PS2). PS1 and PS2 mutation are known to similar effects on the production of amyloid $\beta$ peptide (A$\beta$) and cause of cell death in the Alzheimer's brain. The importance of the alternation of calcium homeostasis in the neuronal cell death by PS1 mutation in a variety of experimental system has been demonstrated. (omitted)

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2003년도 학술대회지
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• pp.281-288
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• 2003

In this paper, seismic responses of large tilting pad journal bearings which have $3\~5$ tilting pads were numerically analyzed. The turbulent lubrication equation, the energy equation and motion equation were solved at each time step. The regime of operation of this bearing is laminar, turbulent and transitional. Also viscosity of working fluid was considered as function of only temperature and inlet pressure build-up was considered. Numerical results for a large tilting pad journal bearing showed journal center, maximum temperature, maximum pressure, friction torque. The relationship of bearing response and seismic intensity are discussed.

• 한국전자파학회논문지
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• 제13권3호
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• pp.257-262
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• 2002

본 논문에서 60 ㎓ 대역용 도파관 급전 radial line slot array (RLSA) 안테나를 설계, 제작하였다. Ewald합 기법과 Shanks 변환을 이용한 효율적인 수치해석으로 RLSA 설계에 필수적인 슬롯을 통한 결합 특성을 계산하였고, 또한 기존의 급전 방식과는 달리 구형 도파관-radial line 변화구조를 이용한 구형 도파관 급전 구조를 사용하였다. 직경 10cm의 크기로 제작된 원형 안테나의 측정 결과 60 ㎓에서 31 ㏈i 의 이득과 38%의 효율을 나타내었다.

• 한국실내디자인학회논문집
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• 제29호
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• pp.3-10
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• 2001

In recent years when the importance of public communication is more emphasized, it has to be noticed that we are now living in the public-initiated age of rhetoric resulted from rapid development of broadcast media. Therefore, the purpose of this treatise is to examine the structural characteristics in the fusion design in order to anaylize the rhetorical expressions as symbols of communication. One of those is intertextuality that expresses metaphorically by bleding and borrowing codes and another is hypertextual space where various texts twines around each other making brand-new and diverse organizations, as the combined allegory with a number of hidden expression. The fusion design may be understood as the Intention to attract gaze to visual messages and as the rhetorical expressions based on compounding aesthetic codes by producing new cultural meanings. If fusion, a phenomenon which represents the 21st century, not a passing fad of cultural mixture, is ready to take the initiative, the design has to be groped for that creates a synergy effect.

• 한국통신학회논문지
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• 제27권3A호
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• pp.284-289
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• 2002

본 논문에서는 MRF(Markov Random field)를 이용해 영상을 분할할 때, 에너지 최적화를 위해 사용되는 simulated annealing의 수렴속도를 개선하는 방법을 제안하였다. Simulated annealing은 잡음이 포함된 영상이나, 텍스쳐 영상에서 좋은 분할 결과를 나타내지만, 수렴속도가 길다는 단점이 있다. 본 논문에서는 수렴속도를 개선하기 위해 픽셀 레이블의 초기값을 픽셀의 intensity에 따라 adaptive하게 부여하여 simulated annealing을 수행하였다. 이 방법으로 모의실험을 수행한 결과, 기존의 방법보다 수렴 속도를 크게 향상시킬 수 있었다.