• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.986-993
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• 2001

Translation Initiation in prokaryotes involves the formation of a 30 S preinitiation complex, in which translation initiation factors play a role in the stimulation of fMet-tRNA (fMet) binding. However, the specific function and precise mechanism of initiation factor IF1 are still unclear. One a functionally active factor with a high purity. In the present study a large quantity of active IF was rapidly purified, obtained by the overexpression of the infA gene, and then used for a functional study. The induction of infA did not appreciably affect the growth rate of the protease-deficient strain E. coli AR68 harboring the IF1 overproducing plasmid. The level of IF1 obtained was approximately $1-2\%$ of the total cell protein, which enabled the yield of highly purified IF1 (>$98\%$ pure) to be increased to 0.15 mg of IF1/g of cells. The IF1 was isolated within one day by the centrifugatioin of the ribosomal washed fraction, by ammonium sulfate fractionation, chromatography on batch of phosphocellulose, and FPLC Mono S. The overexpressed IF1 was found to be comparable to the factor isolated from normal cells, as determined by migration in NEPHGE/SDS 2-D gels. For binding of fMet-tRNA(fMet) to the 30 S ribosomal subunitis, relatively high levels of binding were obtained when IF2 was present. The addition of IF1 up to 110 pmol proportionally stimulated the binding to a variable extent. This IF1-dependent stimulation of the 30 S preinitiation complex formation demonstrated that IF1 would appear to be exclusively essential for promoting the initiation phase of protein synthesis.

• Journal of the Korean Home Economics Association
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• v.31 no.1
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• pp.181-192
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• 1993

This study was undertaken to investigate the factorial structure of clothing purchase motives, and to examine the relationship between clothing purchase motives, self-concept, and post-purchase clothing satisfaction of males. Questionnaire was comprised of three sections: 25 Likert type statements of clothing purchase motive measure; post-purchase clothing satisfaction measure; and three self-concepts measure(physical, personal, and social self-concept). For the measurement of self-concepts, a portion of‘Self-Concept Scale’by Wonshik Chung was used. Samples were 312 male white collar workers in Seoul, whose age were ranged from 20 to 39. the data were analyzed using factor analysis, Cronbach's alpha reliability coefficient, Pearson's correlation coefficient, and multiple regression analysis. The results of the study were the followings: 1. Six factors of clothing purchase motives derived by factor analysis. 21 items having a factor loading of ± .40 or higher were given consideration in the interpretation of each factor: F.1‘clothing dsign’; F.2‘fashion and impulsive buying’, F.3‘clothing utility’; F.4‘others influence’: F.5‘financial margin’; F.6‘clothing’2. Male consumers perceived the purchase motive of‘clothing design’to be the most important,‘clothing deficiency’the second important, and‘others influence’the least important. 3. Physical, personal, and social self-concept had positive relations to the purchase motive of‘clothing design’and negative relations to the‘clothing deficiency’. Physical self-concept had negative relation to the motive of‘others influence’. 4. Male consumers high in physical, personal, and social self-concept had more positive attitude in the evaluation of post-purchase clothing satisfaction. 5. Post-purchase clothing satisfaction was influenced most by the purchase motive of‘clothing design’, and next by physical self-concept, and age. Age of subjects had negative relation to post-purchase clothing satisfaction. The explanatory power of the 3 variables was 19.9%.

• Speech Sciences
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• v.8 no.1
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• pp.143-157
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• 2001

This paper focused on glottal parameters contributing to the perception of loud voices because energy of a voice is not the only effective factor. We used a formant synthesizer to synthesize loud voices. We divided F0 tilt (the tilt of F0 contour), SQ (Speed Quotient), OQ (Open Quotient) and TL (spectral Tilt Level) into three levels to get different combinations with default values for the other synthesizer parameters. Analysis of listening tests indicated that F0 tilt, SQ, OQ and TL in descending order had significant influence on the perception of loud voices. F0 tilt had a far more significant effect than the others. The influence of SQ increased greatly with the exclusion of F0 tilt as a factor. The interaction between parameters was not significant.

• Journal of the Korea Safety Management & Science
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• v.11 no.3
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• pp.209-216
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• 2009

A development in method of evaluating the measurement precisions using approximate F tests and variance components from expected mean square (EMS) is investigated. The research proposes three-factor mixed measurement models with the fixed and random factors. Unrestricted and unconstrained design work was rarely studied, while restricted and constrained designs have been significantly discussed. The unrestricted and unconstrained designs assume to be an independence of interaction. The proposed evaluation method about the measurement precisions can be extended to four-factor random measurement model or mixed measurement model. The study also presents the three evaluation indexes of precisions such as R&RTR (Reproducibility & Repeatability-To-Total Precision Ratio), PTR (Precision-To-Tolerance Ratio), and SNR (Signal-To-Noise Ratio). Numerical examples are proposed to evaluate the approximate F tests with Satterthwaite degrees of freedom and three indexes using the measurement precisions from EMS.

• Journal of the Korean Ceramic Society
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• v.25 no.1
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• pp.1-6
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• 1988

The IR edge absorption of the important pure components of ZrF4-based glasses, including two previously uncharacterized components(ZrF4 and AlF3), has been measured. Values of the glass IR edge absorption coefficients have been calculated from these results, assuming additivity, and are compared with experimental glass absorption coefficients. The calculated ${\alpha}$ values are about a factor of two lower than the experimental values for ZrF4-based glasses, and the ZrF4 makes the largest contribution to the calculated ${\alpha}$ value.

• Clinical and Experimental Pediatrics
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• v.59 no.6
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• pp.271-275
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• 2016

Purpose: A limited number of studies have examined the link between F-wave abnormalities and clinical presentation in pediatric Guillain-$Barr{\acute{e}}$ syndrome (GBS). Therefore, this study examined the importance of F-wave abnormalities as a prognostic factor in pediatric GBS patients. Methods: The records and electrodiagnostic studies (EDS) of 70 GBS patients were retrospectively evaluated, and divided into 2 groups according to the results of EDS. Group A (n=33) presented with F-wave abnormalities, and group B (n=26) exhibited normal findings. We compared laboratory reports, clinical features, response to treatment, and prognosis between the 2 groups. Results: Motor weakness was the most frequently observed symptom for either group. Clinically, the incidence of fever and upper respiratory symptoms differed between the 2 groups, while the prevalence of abnormal deep tendon reflex (DTR) was significantly higher in group A than B (P<0.05). Patients diagnosed with GBS had received intravenous immunoglobulin treatment: 94% in group A and 58% in group B. Furthermore, significantly greater numbers of patients in group A showed H-reflex abnormalities and poor prognosis compared with group B (P<0.05). Conclusion: This study demonstrated that F-waves are a clinically important prognostic factor in GBS. F-wave abnormalities were associated with abnormal DTR and poor prognosis in patients. Limited studies have examined the link between F-wave abnormalities and clinical results; therefore, further randomized controlled studies are needed to confirm the clinical characteristics and efficacy of treatments.

• Journal of the Korean Society of Costume
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• v.25
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• pp.233-247
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• 1995

The objectives of this study were to classify the contents of clothing involvement, to group elderly women into life style types. A method of this study was face to face re-search and questionnaire. Questionnaire was comprised of four sections : 18 Likert type items of clothing involvement measure ; 26 Likert type items of life style measure ; 3 items of clothing purchase measure ; and 3 demographic variables. Samples were 215 elderly women(60∼79 years of age) in Seoul, Korea. The data were analyzed using factor analy-sis, cluster analysis, one-way ANOVA, Dun-can's multiple range test,χ2 test. The results of the study were the follow-ings. 1. Four factors of clothing involvement derived by factor analysis : F.1 'clothing pleasure'; F.2 'clothing symbolism' ; F.3 'perceived risk in clothing purchase' ; F.4 'clothing interest'. 2. Four factors of life style derived by factor analysis : F.1 'active-leisure';F.2 'confidence oriented';F.3 'appearance interest';F.4 'house-work interest and community conciousness'. Three types of life style were defined by the cluster analysis of the 4 factors : T.1 'passive stag-nation'; T.3'outside activity'. 3. There were significant differences in clothing involvement factors according to life style types. Outside activity type perceived 'clothing pleasure' highest level among 3 life style types. Outside activity type and house-work and positive living type perceived 'cloth-ing symbolism' and 'clothing interest' higher level than did passive stagnation types. 4. Elderly women high in educational level were more distributed in outside activity type and the low in educational level in passive stagnation types. 5. There were significant relationships be-tween life style types and source of a clothing allowance, clothing purchase frequency, and a companion of dress store.

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2403-2409
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• 2014

Absolute isotope ratio is a critical constituent in determination of atomic weight. To measure the absolute isotope ratio using a mass spectrometer, mass discrimination factor, $f_{MD}$, is needed to convert measured isotope ratio to real isotope ratio of gas molecules. If the $f_{MD}$ could be predicted, absolute isotope ratio of a chemical species would be measureable in absence of its enriched isotope pure materials or isotope references. This work employed gravimetrically prepared isotope mixtures of argon (Ar) to obtain $f_{MD}$ at m/z of 40 in the magnetic sector type gas mass spectrometer (gas/MS). Besides, we compare the nitrogen isotope ratio of nitrogen trifluoride ($NF_3$) with that of nitrogen molecule ($N_2$) decomposed from the same $NF_3$ thermally in order to identify the difference of $f_{MD}$ values in extensive m/z region from 28 to 71. Our result shows that $f_{MD}$ at m/z 40 was $-0.044%{\pm}0.017%$ (k = 1) from measurement of Ar artificial isotope mixtures. The $f_{MD}$ difference in the range of m/z from 28 to 71 is observed $-0.12%{\pm}0.14%$ from $NF_3$ and $N_2$. From combination of this work and reported $f_{MD}$ values by another team, IRMM, if $f_{MD}$ of $-0.16%{\pm}0.14%$ is applied to isotope ratio measurement from $N_2$ to $SF_6$, we can determine absolute isotope ratio within relative uncertainty of 0.2 %.

• BMB Reports
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• v.52 no.5
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• pp.336-341
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• 2019

The cGAS-STING pathway plays an important role in pathogen-induced activation of the innate immune response. The 29-kDa amino-terminal fibronectin fragment (29-kDa FN-f) found predominantly in the synovial fluid of osteoarthritis (OA) patients increases the expression of catabolic factors via the toll-like receptor-2 (TLR-2) signaling pathway. In this study, we investigated whether 29-kDa FN-f induces inflammatory responses via the cyclic GMP-AMP synthase (cGAS)/stimulator of interferon gene (STING) pathway in human primary chondrocytes. The levels of cGAS and STING were elevated in OA cartilage compared with normal cartilage. Long-term treatment of chondrocytes with 29-kDa FN-f activated the cGAS/STING pathway together with the increased level of gamma-H2AX, a marker of DNA breaks. In addition, the expression of pro-inflammatory cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF/CSF-2), granulocyte colony-stimulating factor (G-CSF/CSF-3), and type I interferon ($IFN-{\alpha}$), was increased more than 100-fold in 29-kDa FN-f-treated chondrocytes. However, knockdown of cGAS and STING suppressed 29-kDa FN-f-induced expression of GM-CSF, G-CSF, and $IFN-{\alpha}$ together with the decreased activation of TANK-binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3), and inhibitor protein ${\kappa}B{\alpha}$ ($I{\kappa}B{\alpha}$). Furthermore, NOD2 or TLR-2 knockdown suppressed the expression of GM-CSF, G-CSF, and $IFN-{\alpha}$ as well as decreased the activation of the cGAS/STING pathway in 29-kDa FN-f-treated chondrocytes. These data demonstrate that the cGAS/STING/TBK1/IRF3 pathway plays a critical role in 29-kDa FN-f-induced expression of pro-inflammatory cytokines.

• Journal of Korean Society for Atmospheric Environment
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• v.6 no.2
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• pp.155-160
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• 1990

During the period from Mar. 1985 to Feb. 1988, airborne particulate matters were collected and size fractionated by the ANdersen high volume air sampler in Seoul. The concentrations of several heavy metals (Pb, Cu, Zn, Fe, Mn) and benzo(a)pyrene were determined to investigate the size distributions and seasonal variations. And with respect to seven components in the total suspended particulate (TSP), the factor analysis was performed for three groups such as the coarse particles (> 2 $\mu$m), fine particles (< $\mu$m) and TSP. As a result of factor analysis by using the varimax method, the chemical components in the TSP were able to characterize with two principal factors. The first factor, F1 was considered to be a factor indicating the contribution of natural sources and the second factor, F2 was a factor indicating the degree of artificial sources. Each components in the TSP was divided into two main groups of components originated from soil and/or road dust and pollutants originated from automobiles and/or human work.