• Journal of Soil and Groundwater Environment
• /
• v.19 no.3
• /
• pp.25-32
• /
• 2014

The amount of TPH contaminated soil treated at off-site remediation facilities is ever increasing. For the recycle of the treated-soil on farmlands, it is necessary to restore biological and physico-chemical soil characteristics and to remove residual TPH in the soil by an economic polishing treatment method such as phytoremediation. In this study, a series of experiments was performed to select suitable plant species and to devise a proper planting method for the phyto-restoration of TPH-treated soil. Rye (Secale cereale) was selected as test species through a germination test, among 5 other plants. Five 7-day-old rye seedlings were planted in a plastic pot, 20 cm in height and 15 cm in diameter. The pot was filled with TPH-treated soil (residual TPH of 1,118 mg/kg) up to 15 cm, and upper 5 cm was filled with horticulture soil to prevent TPH toxic effects and to act as root growth zone. The planted pot was cultivated in a greenhouse for 38 days along with the control that rye planted in a normal soil and the blank with no plants. After 38 days, the above-ground biomass of rye in the TPH-treated soil was 30.6% less than that in the control, however, the photosynthetic activity of the leaf remained equal on both treatments. Soil DHA (dehydrogenase activity) increased 186 times in the rye treatment compared to 10.8 times in the blank. The gross TPH removal (%) in the planted soil and the blank soil was 34.5% and 18.4%, respectively, resulting in 16.1% increase of net TPH removal. Promotion of microbial activity by root exudate, increase in soil permeability and air ventilation as well as direct uptake and degradation by planted rye may have contributed to the higher TPH removal rate. Therefore, planting rye on the TPH-treated soil with the root growth zone method showed both the potential of restoring biological soil properties and the possibility of residual TPH removal that may allow the recycle of the treated soil to farmlands.

• Korean Journal of Veterinary Research
• /
• v.36 no.4
• /
• pp.973-990
• /
• 1996

To investigate diagnostic imaging of cystitis and to apply it to the small animal practice, ultrasonogram of urinary bladder with moderate distension(4ml/kg) and with complete distension(9ml/kg) to evaluate the irregularity and thickness of bladder, radiography and histopathological examination were performed after experimental cystitis induction. On double contrast cystography, mucosal membrane of the urinary bladder was smooth and thickening urinary bladder wall was not found before cystitis induction. At 3rd day post induction(PI), mucosal irregularity was noted at the cranioventral region of the urinary bladder. Thickening of the urinary bladder wall and filling defect was observed as well. Cystographic findings of 7, 10, 15, 21 day PI were similar to that of 3rd day PI. On ultrasonographic findings of the mural thickness in induction group, it was ascertain that the mural thickness with moderate distention was more thickened than with complete distention at transverse scan. Ultrasonographic findings at longitudinal scan were similar to those of transverse scan. On ultrasonographic findings of mucosal irregularity in induction group, from PI to 7 day PI, mucosal irregularity with moderate distention was more irregular than mucosal irregularity with complete distention. At 10 day PI, there was similarity between moderate distention and complete distention. On histopathologic examination of a section of urinary bladder taken 3 day PI, the mucosal and submucosa were infiltrated by a mixture of thick inflammatory exudate which was composed of neutrophil, plasma protein, bacterial colony and necrotic cells. Congestion, hemorrhage and edema were also observed in the submucosa. At 7th day PI, the mucosal change was similar to that of 3rd day PI, but neovascularization and fibroplasia were observed in the submucosa. At 15th and 21th day PI, mild hyperplasia of mucosal epithelium was observed in the mucosa. Deposition of collagen, neovascularization and severe diffuse infiltration of lymphocyte were observed. These results suggest that ultrasonographic examination with moderate distention is considered to be a more simple, rapid, noninvasive, sensitive and useful diagnostic method than other diagnostic methods for the diagnosis of the cystitis and the differential diagnosis of urinary tract infection.

• Microbiology and Biotechnology Letters
• /
• v.49 no.2
• /
• pp.225-238
• /
• 2021

CH₄-oxidizing and N₂O-reducing bacterial consortia were enriched from the rhizosphere soils of maize (Zea mays) and tall fescue (Festuca arundinacea). Illumina MiSeq sequencing analysis was performed to comparatively analyze the bacterial communities of the consortia with those of the rhizosphere soils. Additionally, the effect of root exudate on CH₄ oxidation and N₂O reduction activities of the microbes was evaluated. Although the inoculum sources varied, the CH₄-oxidizing and N₂O-reducing consortia derived from maize and tall fescue were similar. The predominant methanotrophs in the CH₄-oxidizing consortia were Methylosarcina, Methylococcus, and Methylocystis. Among the N₂O-reducing consortia, the representative N₂O-reducing bacteria were Cloacibacterium, Azonexus, and Klebsiella. The N₂O reduction rate of the N₂O-reducing consortium from maize rhizosphere and tall fescue rhizosphere increased by 1.6 and 2.7 times with the addition of maize and tall fescue root exudates, respectively. The CH₄ oxidization activity of the CH₄-oxidizing consortia did not increase with the addition of root exudates. The CH₄-oxidizing and N₂O-reducing consortia can be used as promising bioresources to mitigate non-CO₂ greenhouse gas emissions during remediation of oil-contaminated soils.

• Korean Journal of Veterinary Service
• /
• v.45 no.2
• /
• pp.79-86
• /
• 2022

Reproductive disorders in cows cause economic loss in livestock farms. This study was carried out to investigate the incidence of endometriosis in the uterine of Korean indigenous cow (Hanwoo). In the present study, the uterine of 25 cows was provided by the slaughterhouse. As a result on a visual examination of the uterus, 18 out of 25 were visually normal, and 7 uteruses (28%) appeared rather pale and showed purulent or mucosal symptoms in Uterine horn. However, the results of hematological analysis showed that both RBC and WBC were normal and showed no signs of systemic inflammation, indicating 7 cows showed asymptomatic endometriosis. The inflammatory uterus (28%) showed a wide range of pathological conditions that elicit an inflammatory response, such as serous exudate and bleeding. Histological and microscopic analysis in the inflammatory group demonstrated that there was swelling of the uterine glands, and neutrophil, basophil, and lymphocyte appeared in the uterine gland. Moreover, plasma cells and hemosiderin-laden macrophages were increased in the endometrial stroma, which lead to inhibit pregnancy by suppression of the synthesis of pregnancy hormones, and the appearance of hemosiderin-laden macrophages is an indicator of intracellular bleeding. In summary, hematologically, it is a normal diagnosis in Korean indigenous cows, however, when the uterus was extracted and investigated microscopically, the asymptomatic endometriosis were evident. In order to achieve the goal of healthy cow management and breeding within 2 weeks after birth, cows' uterus should be washed, disinfected, and through thorough the hygiene management, it aims to prevent asymptomatic endometriosis to produce healthy offspring and reduce the breeding interval.

• The Journal of Internal Korean Medicine
• /
• v.44 no.2
• /
• pp.167-177
• /
• 2023

Background: Pressure injuries are localized areas of damage to the skin and/or underlying tissue, usually over a bony prominence due to pressure. Cerebrovascular disease increases the risk of pressure injuries due to the immobility caused by physical paralysis. The general approach to managing a patient with pressure injuries should include pain relief, the treatment of the infection, optimizing nutritional intake, proper positioning, and contamination prevention. Nonetheless, the duration of treatment for pressure injuries varies from person to person. Case report: An 80-year-old female intracerebral hemorrhage patient developed a pressure injury. To improve the injury faster, a Radix Astragali pharmacoacupuncture solution was applied to the pressure sore. The pressure injury's width, length, and depth was assessed using a ruler, and the exudate amount and tissue types were assessed. The treatment was performed for 35 days. The rate at which the size of the pressure sore lessened increased since the Radix Astragali pharmacoacupuncture solution was applied to the pressure sore. In addition, the tissue type of the pressure injury improved, and the exudates decreased. There was no significant difference in the Pressure Ulcer Scale for Healing Tool 3.0, since the Radix Astragali pharmacoacupuncture solution was applied to the pressure injury. Conclusion: This clinical case study suggests that the Radix Astragali pharmacoacupuncture solution might be effective in speeding up the healing of pressure injuries.

• Tuberculosis and Respiratory Diseases
• /
• v.39 no.2
• /
• pp.131-140
• /
• 1992

In order to investigate the changes of hydroxyproline amount and pathologic finding in rat lung which were instilled the natural coal and free silica dust intratracheally, the subjects were divided into two groups as follows. The control group was only administered intratracheally 0.5 ml of normal saline, and the experimental groups were instilled at once the turbid solution containing 10 mg, 30 mg and 50 mg of natural coal and free silica dust each, subjects were sacrified at the 3rd and the 20th week each after the experiment. Hydroxyproline amount in the right upper lung was measured by Woessner method and HPLC (modified Dunphy) method, and the pathlogic finding of lung tissue were observed for hematoxylin-eosin staining, Bielschowski method and Masson's trichrome method. The results were as follows. 1) The wet lung weights of all experimental groups excluding in the groups instilled 10 mg and 30 mg of natural coal dust at 3rd week, were significantly increased (p<0.05) compared with control group. The weight in each free silica group was markedly increased (p<0.05) at 20th week compared with the same dose of natural coal dust group, while the weight in the same dose group of free silica dust was increased significantly at 20th week compared with at 3rd week. 2) The amount of hydroxyproline were significantly increased (p<0.05) in the natural coal and free silica groups at 20th week compared with the control groups, and in each experimental group instilled the same kind and dose of dust, its amount was markedly increased (p<0.05) at 20th week compared with at 3rd week. And also the hydroxyproline in 30 mg and 50 mg free silica groups increased markedly (p<0.05) at 20th week compared with the natural coal dust of the same dose. 3) The polymorphonuclear leukocytes, fibroblasts and macrophages in interstitium and alveolar space showed the increasing tendency in the free silica group more markedly than in the natural coal dust group. The exudate in alveolar space at 3rd week was disappeared at 20th week, and pneumoconiotic nodules observed microscopically in all experimental groups at 20th week, while the nodules apeared already at 3rd week in the 30 mg and 50 mg free silica dust groups. The significant increase of Hydroxyproline content in lung tissue and pneumoconiotic nodule formation in experimental groups found in this study indicate that the observation period, dust amount and kind of dust is important factors associated with pneumoconiosis. And these findings were generally more severe in free silica dust groups than in natural coal groups.

• The Korean Journal of Mycology
• /
• v.39 no.1
• /
• pp.68-77
• /
• 2011

80% methanol and 0.9% neutral saline soluble and hot water substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting bodies of Grifola frondosa. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180 and RAW 264.7 at the concentration of 10~2000 ${\mu}g/ml$, but crude polysaccharides from Fr. NaCl was slightly toxic to HT-29 and NIH3T3 at the concentration of 2000 ${\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of 25.0~52.9% in mice previously inoculated with Sarcoma 180. Fr. HW increased the numbers of spleen cells by 1.3 fold at the concentration of 200 ${\mu}g/ml$ compared with control. Fr. NaCl improved the immuno-stimulating activity of B lymphocyte by increasing the alkaline phosphatase activity by 1.5 fold compared with control at the concentration of 200 ${\mu}g/ml$. 10~14 ${\mu}M$ of nitric acid were generated when Fr. NaCl was added to RAW 264.7 at the concentration of 50~500 ${\mu}g/ml$, while the control group produced 4.3 ${\mu}M$ of nitric oxide. The Fr. NaCl, Fr. HW and Fr. MeOH increased the production of TNF-${\alpha}$, IL-$1{\beta}$, Il-2 and IL-6 by more than 1.4 times compared with the control group. The Fr. of MeOH increased the numbers of peritoneal exudate cells and circulating leukocytes by 3.0 and 2.0 folds compared with the control at the concentration of 50 ${\mu}g/ml$, respectively. Therefore, the crude polysaccharides extracted from fruiting bodies of Grifola frondosa could improve antitumor activity of mice.

• The Korean Journal of Mycology
• /
• v.39 no.1
• /
• pp.57-67
• /
• 2011

Agaricus brasiliensis, one of edible mushroom belonging to Basidiomycota, has been used for curing gastric ulcer and stomach cancer of human beings and also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were prepared from fruiting body of the mushroom. ${\beta}$-glucan and total protein contents were identify from fractions of edible mushrooms extract. The ${\beta}$-glucan and protein contents of all fractions of the mushrooms ranged from 21.54~32.31% and 0.16~9.34%, respectively. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HT-29, NIH3T3 and RAW 264.7 at the concentration of 10~2000 ${\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of 18.8~50.6% in mice previously inoculated with Sarcoma 180. Fr. HW increased the numbers of spleen cell by 1.2 fold at the concentration of 200 ${\mu}g/ml$ compared with control. Fr. MeOH and Na improved the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 1.6 fold compared with control at the concentration of 50~500 ${\mu}g/ml$. Fr. Na generated 15.9 ${\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7 at the concentration of 200 ${\mu}g/ml$, while lipopolysaccharide, a positive control, produced 3.7 ${\mu}M$. The Fr. NaCl, Fr. HW and Fr. MeOH increased the secretion of TNF-${\alpha}$, IL-$1{\beta}$, Il-2 and IL-6 by 2.2 times compared with the control group. Fr. Na increased the numbers of peritoneal exudate cells by 4 folds at the concentration of 50mg/kg compared with control. Circulating leukocytes increased by 2.7 folds when Fr. HW from A. brasiliensis was inoculated at the concentration of 50 mg/kg body weight. The hematological and blood chemical analysis of the 3 fractions did not show any difference in blood compositions and enzyme activities compared with the control group (p<0.05). Therefore, the experimental results suggested that crude polysaccharides extracted from A. brasiliensis contain antitumor and immuno-potentiating activities against Sarcoma 180 in ICR mice.

• Korean Journal of Soil Science and Fertilizer
• /
• v.24 no.1
• /
• pp.69-76
• /
• 1991

A pot experiment was conducted to find out the effects of lime and phosphate application on the changes of number of soil microorganisms, indigenous Rhizobium japonicum, nodule formation, and ureide-and amide-N in leaf and stem exudate of soybean plant under uncultivated hillside red earth in very low pH value, organic matter, available phosphate, and cation exchange capacity. The results obtained were summarized as follows : 1. The plant height, stem length, root dry weight and nodule weight were significantly increased with the application of lime and phosphate application than that of control plot. 2. The concentration of amide-N in soybean plant at the 45 days after sowing was obtained as high in order of control>lime> lime+phosphate while the concentration was obtained in order of Iime+phosphate>lime> control at flowering stage 3. However, concentration of ureide-N in the soybean leaf at the 45 days after sowing was obtained as high in order of control>lime>lime+hosphate while reversed concentration was obtained in stem. 4. The number of soil microorgan isms were increased with increase of pH value, available phosphate and soil exchangeable cation. 5. Significantly negative high correlation were obtained with the concentration of Al, Fe in soil and the concentration of amide-and ureide-N in soybean plant at flowering stage while positive correlation was obtained with plant growth and the concentration of ureide-N.

• Journal of Bio-Environment Control
• /
• v.14 no.1
• /
• pp.22-28
• /
• 2005

The use of blankets to preserve heat in oriental melon cultivation is a common practise without artificial heating and warming systems. Efficiency of blanket decreased with annually usage. This experiment was conducted to investigate the effect of double layer nonwoven fabrics on heat conservation, plant growth, fruit quality and yield of oriental melon in greenhouse. The results were compared among the non-woven fabrics of 9+3, 6+6, 6+3 and 12 ounce from transplanting to April 20, 2001, 2002. Night temperature within tunnel was high at 9+3, 6+6, 6+3 and 12 ounce in order. In plant growth, stem length, leaf numbers and exudate, under double layer nonwoven fabrics were better than single layer blanket of 12 ounce especially, 9+3 double layer blanket was the best. Fruit weight, flesh thickness, soluble solid and marketable yield rate remained same in all treatments. Fermented fruit rate was the highest in 12 ounce as $32.9\%,\;19.6\%\;under\;9+3,\;17.1\%\;under\;6+6,\;16.6\%$ under 6+3 double layer nonwoven fabric, respectively. Compared to 2,260kg yield per 10a of 12 ounce single layer nonwoven fabrics, $7\%$ was increased under 9+3 but $3\%\;and\;13\%$ were decreased under 6+6 and 6+3 double layer nonwoven fabrics, respectively. Compared to income, 4,499-thousand-won per 10a, of 12 ounce single layer blanket, $13\%\;and\;3$ were increased under 9+3 and 6+6 double layer nonwoven fabrics, respectively. Whereas, $10\%$ decreased under 6+3 double layer nonwoven fabrics. From this results it is evident that 9+3 double layer nonwoven fabrics was the best for thermokeeping, fruit quality, and was most economic under non heating system.