• Genomics & Informatics
• /
• v.17 no.3
• /
• pp.28.1-28.9
• /
• 2019

Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

• The Transactions of the Korean Institute of Electrical Engineers B
• /
• v.55 no.9
• /
• pp.485-490
• /
• 2006

According to the computerization of railway signaling systems, the interface link between the signaling systems has been replaced by the digital communication channel. At the same time, the importance of the communication link is more pronounced than before. In this paper, new Network-based protocol between railway signaling and SCADA (Supervisory Control and Data Acquisition system) has designed and the overview of designed protocol is briefly represented. And also this paper addresses analysis of newly designed train control systems. Fame error rates of the data transmissions are calculated and compared for the two cases that the CTC (Centralized Traffic Control)/SCADA has an extra data transmission error control (CRC16) besides the inherent error control of the Ethernet and that the CTC/SCADA has no extra data transmission error control. With simulation results it has been verified that the additional error control code contributes to lowering the frame error rate. It will be expected to increase the safety, reliability and efficiency of maintenance of the signaling systems by using the designed protocol for railway signaling system.

• KIISE Transactions on Computing Practices
• /
• v.21 no.3
• /
• pp.239-246
• /
• 2015

QR(Quick Response) Code has been developed to provide greater storage capacity and more functionality compared to 1D bar codes. With the emergence of increasingly mobile devices equipped with cameras such as smart-phones and tablets, QR codes have become very popular and more important in mobile businesses. Typically, most QR codes are used as a URL link for redirecting users to webpages. However, the URL based QR codes are required to be connected over the internet and to be run a server. This can incur unnecessary traffics in the Internet. Furthermore, it is not suitable for the country lagging behind others in its network infrastructure. In this paper, we propose a server-less ${\mu}$-webpage to provide mobile web services and be optimized for the capabilities and limitations of QR Code. We have implemented the ${\mu}$-webpage in Android, and the results showed that the proposed mechanism can provide web-services without requiring extra servers or incurring mobile traffic data compared to the URL-based QR Codes.

• Wind and Structures
• /
• v.20 no.3
• /
• pp.449-468
• /
• 2015

Current wind-resistance designs of large-scale indirect dry cooling towers (IDCTs) exclude an important factor: the influence of the ventilation rate for radiator shutter on wind loads on the outer surfaces of the tower shell. More seemingly overlooked aspects are the effects of various ventilation rates on the wind pressure distribution on the tower surfaces of two IDCTs, and the feature of the flow field around them. In order to investigate the effects of the radiator shutter ventilation rates on the aerodynamic interference between IDCTs, this paper established the numerical wind tunnel model based on the Computational Fluid Dynamic (CFD) technology, and analyzed the influences of various radiator shutter ventilation rates on the aerodynamic loads acting upon a single and two extra-large IDCTs during building, installation, and operation stages. Through the comparison with the results of physical wind tunnel test and different design codes, the results indicated that: the influence of the ventilation rate on the flow field and shape coefficients on the outer surface of a single IDCT is weak, and the curve of mean shape coefficients is close to the reference curve provided by the current design code. In a two-tower combination, the ventilation rate significantly affects the downwind surface of the front tower and the upwind surface of the back tower, and the larger positive pressure shifts down along the upwind surface of the back tower as the ventilation rate increases. The ventilation rate significantly influences the drag force coefficient of the back tower in a two-tower combination, the drag force coefficient increases with the ventilation rate and reaches the maximum in a building status of full ventilation, and the maximum drag coefficient is 11% greater than that with complete closure.

• The Journal of Society for e-Business Studies
• /
• v.19 no.4
• /
• pp.151-168
• /
• 2014

UCI (Universal Content Identifier) as a national digital content identification system for identifying digital content and ICN (Integrated Copyright Number) for copyright management are operated by Korea copyright commission. However, They are not interoperable with international standard identification system such as ISRC (International Standard Recording Code), ISWC (International Standard Works Code), and ISAN (International Standard Audiovisual Number) due to lack of linkage system. Hence, extra works with international standard identification system are needed for international market entry and integrated management of statistical information including content settlement in international market is impossible. In this paper, the domestic/international identification systems are surveyed, and by metadata analysis of identification systems, linkage method with UCI/ICN is proposed. And also by developing the prototype model, research direction to UCI/ICN with international standard identification system is proposed.

• Journal of Korea Society of Digital Industry and Information Management
• /
• v.15 no.4
• /
• pp.51-62
• /
• 2019

Recently, as the number of Smart Phone users increase, the simple payment system has been able to make payments using only card information such as a registered password without extra authorized certificate authentication or input of card information. In this paper, it will examine and analyze simple payment system provided by IT companies and financial institutions and the simple payment system that operates global online payment system by case view of operational direction. Then with this examination, it will study ways to improve the problems with terms of convenience and stability in terms of users. In this paper, it will analyze the inconvenient problem in using the QR code system that recently introduced and will propose solutions. Also, it will propose suggestions to solve inconvenience that caused by system that supports NFC simple payment terminal in Korea is not universalize by analyze case study on the overseas simple payment system. It will also propose opinions on the matters that customer having responsible for event of a small financial accident related to loss or theft when using the simple payment system. Then it will suggest expected requirements to prepare new security technical countermeasures and solve the conditions of meeting expectation satisfaction of users.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.35 no.10
• /
• pp.1405-1411
• /
• 2006

The presence and content of residual hexane in the olive oils were studied. Total 41 olive oils of imported and domestic brands, which were labeled as extra virgin and refined (mixed), were collected from the market. For analysis, electronic nose and headspace SPME-GC/MS were used. Electronic nose equipped with 12 metal oxide sensors was used for the discrimination of odor pattern of olive oils against the different concentrations of hexane. From the results, it is assumed that the contents of residual hexane in the collected olive oils were below 5 ppm. For Qualitative and quantitative analysis of hexane, polydimethylsiloxane (PDMS) fiber was employed for SPME-GC/MS. In the results, the peak of residual hexane was detected in 8 samples from 41 olive oils. But the detected level was no more than 1 ppm that is under the regulation limit (5 ppm) by Korea Food Additive Code.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.51 no.2
• /
• pp.85-91
• /
• 2014

Cloud storage system uses a distributed file system for storing and managing data. Traditional distributed file system makes a triplication of data in order to restore data loss in disk failure. However, enforcing data replication method increases storage utilization and causes extra I/O operations during replication process. In this paper, we propose a data replication method using erasure codes in cloud storage system to improve storage space efficiency and I/O performance. In particular, according to data access frequency, the proposed method can reduce the number of data replications but using erasure codes can keep the same data recovery performance. Experimental results show that proposed method improves performance in storage efficiency 40%, read throughput 11%, write throughput 10% better than HDFS does.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2015.10a
• /
• pp.208-210
• /
• 2015

After the huge explosive accident in port of Tianjin, it is being strengthened on the dangerous goods regulations. This also has been make an extra impact which must meet international standards and even different parts became possible import customs regulations exporting from Korea. Since 2004, internationally in compliance with the enforced the transport of dangerous goods regulations (IMDG Code), but becomes substantial damage to the domestic industry that exports of dangerous goods is a lot of difficulties in the logistics of efficiency due to possible follows only the clearance to the needs of the Chinese Government. In Korea and secure hazardous materials management needs have emerged, but piecemeal ad hoc policy is connected economically costly and unnecessary regulations weighted long-term safe at the same time enables continuation of the development of the only domestic chemical industry to find effective hazardous materials management plan it is.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2010.05a
• /
• pp.882-885
• /
• 2010

In view of the severe energy constraint in sensor networks, it is important to use the error control scheme of the energy efficiently. In this paper, we presented FEC (Forward Error Correcting) codes in terms of their power consumption. One method of FEC is RS (Reed-Solomon) coding, which uses block codes. RS codes work by adding extra redundancy to the data. The encoded data can be stored or transmitted. It could have errors introduced, when the encoded data is recovered. The added redundancy allows a decoder to detect which parts of the received data is corrupted, and corrects them. The number of errors which are able to be corrected by RS code can determine by added redundancy. We could predict the lifetime of RS codes which transmitted at 32 byte a 1 minutes. RS(15, 13), RS(31, 27), RS(63, 57), RS(127,115), and RS(255,239) can keep the days of 138, 132, 126, 111, and 103 respectively.