• The Korean Journal of Physiology and Pharmacology
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• v.28 no.3
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• pp.209-217
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• 2024

In addition to cellular damage, ischemia-reperfusion (IR) injury induces substantial damage to the mitochondria and endoplasmic reticulum. In this study, we sought to determine whether impaired mitochondrial function owing to IR could be restored by transplanting mitochondria into the heart under ex vivo IR states. Additionally, we aimed to provide preliminary results to inform therapeutic options for ischemic heart disease (IHD). Healthy mitochondria isolated from autologous gluteus maximus muscle were transplanted into the hearts of Sprague-Dawley rats damaged by IR using the Langendorff system, and the heart rate and oxygen consumption capacity of the mitochondria were measured to confirm whether heart function was restored. In addition, relative expression levels were measured to identify the genes related to IR injury. Mitochondrial oxygen consumption capacity was found to be lower in the IR group than in the group that underwent mitochondrial transplantation after IR injury (p < 0.05), and the control group showed a tendency toward increased oxygen consumption capacity compared with the IR group. Among the genes related to fatty acid metabolism, Cpt1b (p < 0.05) and Fads1 (p < 0.01) showed significant expression in the following order: IR group, IR + transplantation group, and control group. These results suggest that mitochondrial transplantation protects the heart from IR damage and may be feasible as a therapeutic option for IHD.

• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.3
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• pp.44-58
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• 2013

Objectives: Breast cancer is the most common cancer among women and has rapidly increasing rate annually. At present, western cancer therapies by surgery, radiation, and anticancer drug have not been fully effective. So many interests are given to herbal medicine on cancer treatment recently. This study was designed to investigate the effects of Curcuma longa L. (CL) on MDA-MB-231 human breast cancer cells and DMBA-induced breast cancer in rats. Methods: In this experiment, MDA-MB-231 cells were cultured in cell culture plates. 0.0625, 0.125, 0.25, 0.5, 1.0 mg/ml of CL extract were tested for their anti-proliferative effects on MDA-MB-231 cells by MMT assay. And we induced breast cancer in rats. The changes in tumor's weight, and the effects on proliferations of splenocyte and thymocyte were investigated. Results: CL showed anti-proliferative effects on MDA-MB-231 cells in proportion to concentration of the CL. DMBA-induced breast cancer in rats, tumor's weight of the rat was not statistically significant, but showed a tendency to be reduced in the groups treated with CL. Proliferation rate of the rat's splenocyte and thymocyte increased in proportion to CL. In breast cancer tissue, expression of ER-${\alpha}$ was weakened proportionately to the concentration of the CL. Conclusions: These data suggest that CL can prevent the proliferation of breast cancer, then CL is useful to treat patient with breast cancer.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1529-1540
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• 2011

Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is relatively nil. In the present study, we have validated the interfering effects of siRNA (small interfering RNA) using both quantitative and qualitative gene silencing in buffalo granulosa cells. Qualitative gene knockdown was validated using a fluorescent vector, enhanced green fluorescence protein (EGFP) and fluorescently labeled siRNA (Cy3) duplex. While quantitatively, siRNA targeted against the luciferase and CYP19 mRNA was used to validate the technique. CYP19 gene, a candidate fertility gene, was selected as a model to demonstrate the technique optimization. However, to sustain the expression of CYP19 gene in culture conditions using serum is difficult because granulosa cells have the tendency to luteinize in presence of serum. Therefore, serum free culture conditions were optimized for transfection and were found to be more suitable for the maintenance of CYP19 gene transcripts in comparison to culture conditions with serum. Decline in fluorescence intensity of green fluorescent protein (EGFP) was observed following co-transfection with plasmid generating siRNA targeted against EGFP gene. Quantitative decrease in luminescence was seen when co-transfected with siRNA against the luciferase gene. A significant suppressive effect on the mRNA levels of CYP19 gene at 100 nM siRNA concentration was observed. Also, measurement of estradiol levels using ELISA (enzyme-linked immunosorbent assay) showed a significant decline in comparison to control. In conclusion, the present study validated gene silencing using RNAi in cultured buffalo granulosa cells which can be used as an effective tool for functional analysis of target genes.

• Journal of Fashion Business
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• v.8 no.3
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• pp.70-83
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• 2004

The female image has been changed incessantly with age and has been eminently represented in cosmetic advertisement. The female images are changed from the classical images to the active and professional images with the historical current of cyber, digital, and fusion. These changes in the expression of female image are largely due to the spread of post-modernism, feminism, de-construction (Kim, 1994), digital information revolution, and increase of income. The female images in cosmetic advertisement have been expressed very variously with fashion, marketing target, and characteristics of articles. (Im, 1997) The cosmetic advertisements of pure and graceful images were popular in the past. But nowadays individual image, womanly image, and unchanged beautiful image are in vogue. Individual image is very popular in young generation with very short fashion period. Active career woman image represents passion and beauty with extension of women' social roles. Unchanged beautiful image in modern industrial pollution stands for the desire of keeping the beauty in youth. Brand is very important factor to consumers in purchasing. Brand is no larger the simple concept in the past, and accepted as reflection of the image, social status, service, and life-style(Lee, 1998) consumers are very favorable to foreign cosmetics with the increase of overseas travels and import. This phenomenon is considered as very natural, especially in young generation. To create a successful brand image, the harmony of quality, psychological preference, advertisement, and reasonable price are required. According to the questionnaire research implemented by the subject of college women students in five universities in Seoul, the majority of college women students purchase domestic cosmetic brands in cosmetic specialty stores and depend on the tips of acquaintances. Quality, skin-trouble, and brand are considered as special regards for purchasing. Especially, internet shopping in purchasing marks high growth rate and preference for foreign cosmetics is very ardent. It can be expected that the 21st century is the epoch of various small production different from the mass-production in the 20th century. Female image will be probably expressed with individual, emotional, and professional image in the media of digital, fusion, cyber, and technology culture. It can be said that the tendency of cosmetic purchasing in the future will be more focused on brand image and life style.

• Journal of Korean Elementary Science Education
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• v.17 no.2
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• pp.1-10
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• 1998

The purpose of this study was to evaluate the effects of constructivist instruction on science inquiry skills and science-related attitudes, and to investigate the students' responses to the lesson using constructivist instruction. In this study, 108 students of sixth graders from four classes were intentionally selected from two elementary schools located in Gyungsan city, Kyungsangbukdo. Two classes of 54 students were used by the constructivist teaching program and the other two classes were handled by the traditional teaching program. Two teachers who have twenty years' careers guided the program prepared by researcher to the students and this examination was conducted for 8 weeks (total 20 class hours). In pretest and posttest, both groups were tested in the science inquiry skills and science-related attitudes. An interview and questionnaire for the students analyzed the responses about the constructivist instruction. The major results of this study are as follows: First, in science inquiry skills test, there was no statistical significance between the score of the experimental group and that of the comparative group(p>.05). The ability of formulating problem in the experimental group was significantly higher than that in the comparative group at 0.05 level. In pretest and posttest, the experimental group was statistically significant on classifying, formulating problem, interpreting data, while the comparative group was not significant on those. Second, we can find that the experimental group has more positive effect on forming the desirable science-related attitudes than the comparative group(p<.05). Especially, experimental group is significantly higher the attitudes about the science curriculum than the comparative group at 0.05 level Third, constructivist instruction has more affirmative tendency of the students' learning activity. Constructivist instruction generated much interest and curiosity about science for the students, and this method prompted students' expression and discussion.

• Archives of design research
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• v.16 no.3
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• pp.101-110
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• 2003

People are surrounded with numerous sounds. The sound is generated from nature and people. For example, the sound enables people or animal responsive to instinctive action. Music or fine arts are presented differently by its distinctive medium. On the other hand, those art forms are similar in a way that people uses right side of brain and intuitions for creative effects. Conjunctions between sounds and visual arts have been progressed to data. From art forms in subjective views to art forms using high technology such as the computer, experiments for sounds visualization are practiced constantly. For that reason, intrinsic attributes of sounds in design area and distinctive qualities are discussed in this study. With respect to existing category of studies and consideration of the tendency in recent researches, the object is to propose direction for the study in regards to methodology of design, which is reconstructing visualized expression.

• Journal of environmental and Sanitary engineering
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• v.10 no.3
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• pp.85-98
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• 1995

In order to ensure that all major cations and anions were accurately measured, the quality assurance checks of chemical analysis data by considering ion and conductivity balance of each precipitation sample were performed. To check the quality assurance of chemical analysis data, precipitation samples were collected by wet- only precipitation sampler at Seoul site and their chemical components were analyzed. By checking the problems for the screening methods of chemical analysis data used until recently, the f value expressed as the ratio of the sum of cations and anions equivalent concentration( $\Sigma $C/$\Sigma $A ) was found to be not ap priorate for data screening. Also, the scattering plot between cation and anion equivalent concentrations in each sample was found to show the general tendency of ion balance but was proved to not quantitate the standard of data screening at a set of samples of various concentration levels.4 more appropriate value was therefore required, h value is defined as (A-C)/C for C≥A and ( A-C)/A for C<4. This value was showed to check the ion balance in a viewpoint of quantitative as well as qualitative and to be useful in applying this expression to a measurement data set. However, the standard o( data screening must vary in response to the ion concentration of sample. In this study, the quality assurance of chemical analysis data was checked by considering both the ion balance evaluating by h value and the electrical conductivity. As these quality assurance checks were applied to Seoul data serf 67 valid samples were obtained. The result of statistical summary in the analytical parameter of precipitation samples collected for a certain period was found to be computed in the precipitation volume- weighted mean( VWM) rather than the arithmetic mean( AM), but PH In the VWM of hydrogen ion concentration. The annual VWM of pH values was 5.0(4.9 ∼ 5.1).

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.4
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• pp.447-454
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• 2014

Recently most of functional synthesis methods for quantum circuit realization have a tendency to adopt the declarative functional expression more suitable for computer algorithms, so it's difficult to analysis synthesized quantum functions. This paper presents a new functional representation of quantum circuits compatible with simple architecture and intuitive thinking. The proposal of this paper is a new functional synthesis development by using the control functions as the power of corresponding to affine-controlled quantum gates based on the mathematical substitution of serial-product matrix operation over the target line for the arithmetic and modulo-2 ones between power functions of unitary operators. The functional synthesis algorithm proposed in this paper is useful for the functional expressions and synthesis using both of reversible and irreversible affine-controlled NCV-quantum gates.

• Atmosphere
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• v.25 no.2
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• pp.309-322
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• 2015

The objective of this study is to evaluate the impact of the high resolution topographies and landuses data on simulated meteorological variables (wind speed at 10 m, temperature at 2 m and relative humidity at 2 m) in WRF. We compare the results with WRF simulation using each resolution of the topographies and landuses, and with 37 AWS observation data on the Seoul metropolitan regions. According to results of using high-resolution topography, WRF model gives better topographical expression over domain. And we can separate more detail (Low intensity residential, high intensity residential, industrial or commercial) using high resolution landuses data. The result shows that simulated temperature and wind speed are generally higher than AWS observation data. However, simulation trend with temperature, wind speed, and relative humidity are similar to observation data. The reason for that is that the high precipitation event occurred in CASE 1 and 2. Temperature have correlation of 0.43~0.47 and standard deviation of $2.12{\sim}2.28^{\circ}C$ in CASE 1, while correlation of more than 0.8 and standard deviation of $3.05{\sim}3.18m\;s^{-1}$ in CASE 2. In case of wind speed, correlation have lower than 0.5 and Standard Deviation of $1.88{\sim}2.34m\;s^{-1}$ in CASE 1 and 2. In statistical analysis shows that using highest resolution (U01) results are more close to the AWS observation data. It can be concluded that the topographies and landuses are important factor that affect model simulation. However, the tendency to always use high resolution topographies and landuses data appears to be unjustified, and optimal solution depends on the combination of scale effect and mechanisms of dynamic models.

• Korean Institute of Interior Design Journal
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• v.24 no.1
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• pp.72-81
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• 2015

The change towards meeting diverse customer needs has started to affect the hotel industry as well. Design hotels emerged as customers demanded differentiated lodging experiences they could not fulfill at franchise hotels which were uniform in style everywhere in the world. Design hotels are meant to provide a new concept of space through unique design sensibility and work of designers, which highlighted the role of interior design. This research analyzes the characteristics of indoor space expressed in design hotel common spaces by the French designer Andree Putman, also known as the founder of boutique hotels and the concept of design hotels. As the original founder of the concept of design hotels, Putman's scope of work stretches wide, from interior design to product design. She is also one of the few French designers with a modernist inclination. The research runs a review on previous literature, characteristics of design hotels according to design tendency, and the association with characteristics of indoor space designed by Andree Putman, followed by case analyses extracted by analyses on design characteristics of common spaces she has designed. The analysis reveals that she creates comfortable yet elegant space, using contrast and fusion arising from negotiation of design styles to interpret space, the sense of spatial balance and understated expression of order through symmetrical structures, diverse creation of space through geometric structuring, highlighting indoor space by utilizing lights as objects, and the heterogeneous harmony achieved by contrasting juxtaposition, and understated formativeness. It is expected that study results would be utilized as methods of new designs of interior space in design hotels.