• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.782-787
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• 2015

In this study, we developed an assay system for missense mutations in human phenylalanine hydroxylases (hPAHs). To demonstrate the reliability of the system, eight mutant proteins (F39L, K42I, L48S, I65T, R252Q, L255V, S349L, and R408W) were expressed in a mutant strain (pah^-) of Dictyostelium discoideum Ax2 disrupted in the indigenous gene encoding PAH. The transformed pah - cells grown in FM minimal medium were measured for growth rate and PAH activity to reveal a positive correlation between them. The protein level of hPAH was also determined by western blotting to show the impact of each mutation on protein stability and catalytic activity. The result was highly compatible with the previous ones obtained from other expression systems, suggesting that Dictyostelium is a dependable alternative to other expression systems. Furthermore, we found that both the protein level and activity of S349L and R408W, which were impaired severely in protein stability, were rescued in HL5 nutrient medium. Although the responsible component(s) remains unidentified, this unexpected finding showed an important advantage of our expression system for studying unstable proteins. As an economic and stable cell-based expression system, our development will contribute to mass-screening of pharmacological chaperones for missense PAH mutations as well as to the in-depth characterization of individual mutations.

• Korean Journal of Cognitive Science
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• v.14 no.1
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• pp.41-50
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• 2003

We tested the capability of the pleasure and arousal dimension-based facial expression synthesis system and proposed the most effective interface for it. First, we tried to confirm the adequateness of the dimensional model as a basic structure of the internal states for the system. Fer it, subjects compared the 17 facial expressions on the two axes. The results validated the fundamental hypothesis of the system. Second, we chose 21 representative expressions from the system to test its performance and had subjects rate their similarities. We analyzed these data using multidimensional scaling methods and these results verified the system's reliability. Third, we compared the efficiencies of two interfaces -coordinate values and slide bars- to find the most suitable interface for the system. Subjects synthesise 25 facial expressions with each interface of it. The results showed that the visualization of two dimensional values into Cartesian coordinate is more stable as an input display of facial expression synthesis system based on dimensions.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4560-4570
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• 2022

Dynamic fault tree (DFT) and its related research methods have received extensive attention in safety analysis and reliability engineering. DFT can perform reliability modelling for systems with sequential correlation, resource sharing, and cold and hot spare parts. A technical modelling method of DFT is proposed for modelling ship collision accidents and loss-of-coolant accidents (LOCAs). Qualitative and quantitative analyses of DFT were carried out using the cutting sequence (CS)/extended cutting sequence (ECS) method. The results show nine types of dynamic fault failure modes in ship collision accidents, describing the fault propagation process of a dynamic system and reflect the dynamic changes of the entire accident system. The probability of a ship collision accident is 2.378 × 10^-9 by using CS. This failure mode cannot be expressed by a combination of basic events within the same event frame after an LOCA occurs in a marine nuclear reactor because the system contains warm spare parts. Therefore, the probability of losing reactor control was calculated as 8.125 × 10^-6 using the ECS. Compared with CS, ECS is more efficient considering expression and processing capabilities, and has a significant advantage considering cost.

• Journal of the Korean Data and Information Science Society
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• v.27 no.2
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• pp.395-407
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• 2016

This study aims to apply the G-theory for estimation of reliability of evaluation scores between raters on Taekwondo Poomsae rating categories. Selecting a number of game days and raters as multiple error sources, we analyzed the error sources caused by relative magnitude of error variances of interaction between the factors and proceeded with D-study based on the results of G-study for optimal determination of measurement condition. The results showed below. The estimated outcomes of variance component for accuracy among the Taekwondo Poomsae categories with G-theory showed that impact of error was the biggest influence factor in raters conditions and in order of interaction in subjects and between subjects, also impact of variance component estimation error on expression category was the major influence factor in interaction and in order of the between subjects and raters. Finally, the result of generalizability coefficient estimation via D-study showed that measurement condition of optimal level depend on the number of raters was 8 persons of raters on accuracy category, and stable reliability on expression category was gained when the raters were 7 persons.

• Journal of Fashion Business
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• v.20 no.1
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• pp.53-68
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• 2016

The purpose of this research was to evaluate the possibility of sustainable development of online advertisements conducted by fashion companies. Factors composed of sustainable development indexes of online advertisement that had been developed in previous studies were identified, and then the relevance between purchase intention and advertisement experience was evaluated. An online survey of 573 persons in the 20 to 40 age range who own mobile phone and have experienced online advertisements of a fashion brand or a fashion company was conducted. The data collected from the survey and the results are as follows. First, the validity and reliability from confirmatory factor analysis of six factors (namely, personal information protection, web use infringement, advertisement expression harmfulness, advertisement expression objectivity, emotional responsibility, and environment-friendly) and 21 questions was confirmed. Second, it confirmed that consumers gave low points to the evaluation of sustainable development indexes of online advertisement of fashion companies. In particular, that consumers gave low points with regard to both environmental friendliness and web use infringement. Third, it was identified that personal indexes such as personal information protection, web use infringement, and indexes relating to advertisement expressions do not directly influence the consumer's purchase intention. However, social indexes like emotional responsibility and environmental friendliness do have an influence on the consumer's positive action intention.

• Journal of Engineering Education Research
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• v.17 no.6
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• pp.53-61
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• 2014

This study aims to identify on the possibility of the convergence education in engineering. Delphi survey on a panel of experts was chosen to be the main methodology for this study, drawing the main factors of convergence education in engineering. From Oct. 10 to Nov. 25, 2013, a three-round Delphi survey was implemented to collect data. A panel of eighteen experts were involved in this survey. For statistical processing, descriptive statistics including frequency, percentage, mean and standard deviation were carried out along with internal reliability test on the survey instrument. First, the possible convergence of disciplines in engineering were found to Design, Industrial design, ICT, Health care services, Video media, Business and Administration, Organizational psychology, Sociology, Humanities and Aesthetics. Second, the convergence education factors to be most important demanded creativity, idea generation techniques such as the expression of thought communication skills, technical expertise in the field per line, understanding of basic knowledge, verification of common elements among different disciplines, understanding of other disciplines fusion in engineering, artistry and imagination, etc. Third, requirements for the talented person of convergence were the open mind and enthusiasm, creative imagination, accept the opinions of others, skills capacity as a creative expression, and challenges. Above-mentioned requirements are found to be the necessary elements for convergence education.

• Journal of the Korean Home Economics Association
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• v.39 no.9
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• pp.95-102
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• 2001

The Effects of Self-Esteem and Sociocultural Attitude toward Appearance on Clothing Behavior. The purpose of this study was to investigate to relation between self-esteem and sociocultural attitude toward appearance and clothing behavior i.e. individuality/self expression, body improvement, social approval, sex appeal. Measurements were included Rosenberg's self-esteem, Thompson's sociocultural attitude toward appearance questionnaire and clothing behavior. The data were collected from 498 female university students in Seoul and Kyongki Province. The collection works were undertaken between the 15th of June and 30th of June, 2001, The data were analyzed using factor analysis, reliability test, analysis of variance, and multiple regression analysis. The results of this study were as follows : 1) Three dimensions of sociocultural attitude toward appearance were identified: internalization, social awareness, and concrete awareness. 2) Self-esteem and internalization of sociocultural attitude toward appearance had an effect on clothing behavior i.e. individuality/self expression, body improvement, social approval and sex appeal. Social awareness of sociocultural attitude toward appearance had an effect on body improvement, social approval, and sex appeal.

• Korean Journal of Adult Nursing
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• v.16 no.3
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• pp.366-377
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• 2004

Purpose: This study is a descriptive research study to develop the Nurse's Humor Questionnaire, and then analyze humor's appreciation and use by Korean nurses. Method: The three stages used to develop the questionnaire were to make a conceptual framework, make an open questionnaire through the 2nd preliminary inquiry, and make a structured questionnaire based on collected statements through the developed open questionnaire. The subjects that carried out completely a structured questionnaire were 337 nurses. Result: 1) The final developed questionnaire consists of 6 domains and 67 items. The reliability of 'When feeling humor's expression'(${\alpha}=0.7079$), 'The barrier factors of humor's use'(${\alpha}=0.7895$), and 'Humor's values' (${\alpha}=0.8336$) was high. 2) The results related to Humor's values of nurses that showed significant differences were as follow: (1) age, marital status, and current position (2) a negative correlation between stress and humor's values (3) humor's sources: daily life and conversation (4) frequency of used humor (5) types of used humor: word humor, imitation, adequate situation and technical terms, and (6) when using humor's expression: tense atmosphere, awkward atmosphere and adequate situation. Conclusion: The developed tool can be used for understanding nurse's humor. This will help to develop the humor program as a nursing intervention for health promotion.

• Journal of IKEEE
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• v.25 no.4
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• pp.613-618
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• 2021

Facial expression recognition has long been established as a subject of continuous research in various fields. In this paper, the relationship between each landmark is analyzed using the features obtained by calculating the distance between the facial landmarks in the image, and five facial expressions are classified. We increased data and label reliability based on our labeling work with multiple observers. In addition, faces were recognized from the original data and landmark coordinates were extracted and used as features. A genetic algorithm was used to select features that are relatively more helpful for classification. We performed facial recognition classification and analysis with the method proposed in this paper, which shows the validity and effectiveness of the proposed method.

• Journal of Aerospace System Engineering
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• v.12 no.3
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• pp.53-57
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• 2018

The Monte Carlo simulation (MCS) method and the Guide to the Expression of Uncertainty in Measurement (GUM) are the most widely used approaches for uncertainty estimation. In this paper, MCS and GUM were used to estimate the confidence of MOI measurement equipment developed in-house. According to the results, the GUM estimated uncertainty was slightly underestimated compared to the MCS method. This difference is due to the approximation used by GUM. MOI uncertainties estimated by both methods were less than 1% of the estimate, which shows the high measurement reliability of the developed MOI measurement system.