• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.15 no.26
• /
• pp.85-93
• /
• 1992

수작업 또는 컴퓨터에 의하여 신뢰도 방정식을 얻을 때 반드시 그것의 옳고 그름을 검정해 볼 필요가 있다. 본 논문에서는 결합구조에 있어서 이항변수를 사용한 이항 성분으로 이항 시스템을 설계하는 법을 제시하였고 검정하기 위하여 검정 방법 두 가지를 신뢰도 방정식의 정확성을 제안하였다. 아울러 몇 가지 예를 들어 계산상의 효과가 배가되었음을 입증하였다.

• Journal of Animal Science and Technology
• /
• v.57 no.5
• /
• pp.18.1-18.8
• /
• 2015

Background: Quantitative real time reverse transcription PCR (qRT-PCR) is one of the most important techniques for gene-expression analysis in molecular based studies. Selecting a proper internal control gene for normalizing data is a crucial step in gene expression analysis via this method. The expression levels of reference genes should be remained constant among cells in different tissues. However, it seems that the location of cells in different tissues might influence their expression. The purpose of this study was to determine whether the source of mesenchymal stem cells (MSCs) has any effect on expression level of three common reference genes (GAPDH, ${\beta}$-actin and ${\beta}2$-microglobulin) in equine marrow- and adipose-derived undifferentiated MSCs and consequently their reliability for comparative qRT-PCR. Materials and methods: Adipose tissue (AT) and bone marrow (BM) samples were harvested from 3 mares. MSCs were isolated and cultured until passage 3 (P3). Total RNA of P3 cells was extracted for cDNA synthesis. The generated cDNAs were analyzed by quantitative real-time PCR. The PCR reactions were ended with a melting curve analysis to verify the specificity of amplicon. Results: The expression levels of GAPDH were significantly different between AT- and BM-derived MSCs (p < 0.05). Differences in expression level of ${\beta}$-actin (P < 0.001) and B2M (P < 0.006.) between MSCs derived from AT and BM were substantially higher than GAPDH. In addition, the fold change in expression levels of GAPDH, ${\beta}$-actin and B2M in AT-derived MSCs compared to BM-derived MSCs were 2.38, 6.76 and 7.76, respectively. Conclusion: This study demonstrated that GAPDH and especially ${\beta}$-actin and B2M express in different levels in equine AT- and BM-derived MSCs. Thus they cannot be considered as reliable reference genes for comparative quantitative gene expression analysis in MSCs derived from equine bone marrow and adipose tissue.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.21 no.3
• /
• pp.128-138
• /
• 2011

Objectives: It has been pointed out that there are problems in handling and managing chemicals due to trade secrets of Material Safety Data Sheet(MSDS). To date, some company intentionally leave out of several parts and use an inaccurate expression in MSDS. In particular, with the result of the inspection of the government and labour community, it is required that the 2nd section in MSDS included the composition and information on ingredients has to be provided with comprehensive expression to secure more reliability. Methods: Therefore, this study is aimed: 1) to recognize the current status of trade secrets of MSDS in workplaces with both domestic manufacturers of chemical products and multinational corporations; 2) to make contributions to prevention of the industrial accidents by providing the accurate information of MSDS; 3) to improve the risk communication system related with chemicals; 4) to impress workers on the importance of right of known for MSDS. With the result we analyzed the status of trade secrets in MSDS in 73 companies, such as petrochemistry production, paint production, metal processing oil production, detergent production, and international company related with chemicals, we have found that 38,150 (45.5%) have the trade secrets parts in the total number of 83,832 in MSDS. Also, based on the 288 MSDS gathered by the Occupational Safety and Health Research Institute (OSHRI) from 2005 to 2009, 41.7% of the MSDS are classified into the trade secrets. Conclusions: Therefore, to procure an assurance system of MSDS, we suggest that a MSDS picking up and checking system be legislated in the Occupational Safety and Health Act to protect workers from the unidentified chemical hazards due to the secret trade of MSDS.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.8
• /
• pp.2394-2398
• /
• 2013

Nicotine is the main component of environmental tobacco smoke, and its presence in indoor air is widely used as a secondhand-smoke indicator. Environmental tobacco smoke is a major source of indoor air pollution, but sufficient investigation of the uncertainty of its measurement, which mirrors the reliability of nicotine measurement, has not been performed. We calculated the uncertainty of measurement of indoor air nicotine concentration at low, medium, and high concentrations of 11.3798, 10.1977, $98.3768{\mu}g/m^3$, respectively, and we employed the Guide to the Expression of Uncertainty in Measurements (GUM), proposed by the International Organization for Standardization (ISO). The factors considered in determining the uncertainty were uncertainty of the calibration curve (calibration curve and repeated measurements), desorption efficiency, extraction volume, and sampling airflow (accuracy and acceptable limits of flowmeter). The measurement uncertainty was highest at low concentrations; the expanded measurement uncertainty is $0.9435{\mu}g/m^3$ and is represented as a relative uncertainty of 63.38%. At medium and high (concentrations, the relative uncertainty was 13.1% and 9.1%, respectively. The uncertainty of the calibration curve was largest for low indoor nicotine concentrations. To increase reliability of measurement in assessing the effect of secondhand smoke, measures such as increasing the sample injection rate ($1{\mu}L$ or more), increasing sampling volume to increase collected nicotine, and using gas chromatography-mass spectrometry (GC/MS) or GC/MS/MS, which has a lower quantitation threshold, rather than gas chromatography with nitrogen phosphorous detector, should be considered.

• Journal of Periodontal and Implant Science
• /
• v.51 no.1
• /
• pp.18-29
• /
• 2021

Purpose: The aim of this study was to compare the characteristic expression patterns of advanced periodontitis in 2 cohort data sets analyzed using different microarray platforms, and to identify differentially expressed genes (DEGs) through a meta-analysis of both data sets. Methods: Twenty-two patients for cohort 1 and 40 patients for cohort 2 were recruited with the same inclusion criteria. The 2 cohort groups were analyzed using different platforms: Illumina and Agilent. A meta-analysis was performed to increase reliability by removing statistical differences between platforms. An integrative meta-analysis based on an empirical Bayesian methodology (ComBat) was conducted. DEGs for the integrated data sets were identified using the limma package to adjust for age, sex, and platform and compared with the results for cohorts 1 and 2. Clustering and pathway analyses were also performed. Results: This study detected 557 and 246 DEGs in cohorts 1 and 2, respectively, with 146 and 42 significantly enriched gene ontology (GO) terms. Overlapping between cohorts 1 and 2 was present in 59 DEGs and 18 GO terms. However, only 6 genes from the top 30 enriched DEGs overlapped, and there were no overlapping GO terms in the top 30 enriched pathways. The integrative meta-analysis detected 34 DEGs, of which 10 overlapped in all the integrated data sets of cohorts 1 and 2. Conclusions: The characteristic expression pattern differed between periodontitis and the healthy periodontium, but the consistency between the data sets from different cohorts and metadata was too low to suggest specific biomarkers for identifying periodontitis.

• The Journal of Korean Physical Therapy
• /
• v.26 no.2
• /
• pp.90-96
• /
• 2014

Purpose: The aim of the study was to develop the Korean version of the Working Alliance Inventory (KWAI) and to assess reproducibility of both its therapist and client forms. Methods: The 12-item WAI was translated into Korean. The translation procedure followed the guidelines proposed by Beaton et al. (2000). Development of KWAI involved its translation, back-translation, and pilot testing of the pre-final version to establish Korean cultural adaptation of the original version of WAI. For test-retest reliability, therapists (N=30) and clients (N=47) completed the questionnaire on two different occasions. The interval between occasions was two to seven days, depending on subject availability. Data were collected from clinicians working in general hospitals and private clinics, and clients who had received treatment within three months. The intra-class correlation (ICC ($_{2.1}$)) was calculated for assessment of the reproducibility of the translated questionnaire. Results: The test-retest ICC ($_{2.1}$) of the client form and the therapist form was 0.92 (95%CI:0.86-0.95) and 0.94 (95%CI:0.89-0.97), respectively. Answers to items 2, 8, and 11 showed a tendency to be omitted due to ambiguity of meaning in Korean. Idiomatic expression was employed rather than word-to-word translation to have clear meaning of those items. Conclusion: The KWAI was successfully translated and adapted for applications to Korean clients and therapists, with a satisfactory level of reliability. Therefore, it can be suggested that the KWAI is useful in providing a reliable assessment of working alliance between therapists and clients.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.22 no.2
• /
• pp.151-157
• /
• 2022

In this paper, the performance of various blockchain consensus algorithms was compared and analyzed as a method to increase the transaction cost and processing time during NFT transactions and to increase the transaction stability requirements that occur during smart contract execution. Network reliability and TPS are evaluation items for performance comparison. TPS and the stability of the Consensus algorithm are presented for three evaluation items. In order to establish a standardized expression for each evaluation item, the reliability of the node and the success rate of the smart contract were considered as variables in the calculation formula, and the performance of the consensus algorithm of the three groups, PoW/PoS, Paxos/Raft and PBFT, was compared under the same conditions. / analyzed. As a result of the performance evaluation, the network reliability of the three groups was similar, and in the case of the remaining two evaluation items, it was analyzed that the PBFT consensus algorithm was superior to other consensus algorithms. Through the performance evaluation equations and results of this study, it was analyzed that when the PBFT consensus processing process is reflected in the consensus process, the network reliability can be guaranteed and the stability and economic efficiency of the consensus algorithm can be increased.

• Journal of Digital Forensics
• /
• v.13 no.2
• /
• pp.125-134
• /
• 2019

The process of identifying system behavior or user behavior from data collected during the digital forensics investigation is essential. In the case of PCs, there are many different types of system behavior or remaining logs depending on the operating system, and the analysis results of the de facto forensics tools that analyze the data are different. Because the reliability of a tool in the digital forensics field is an important factor, cross-analysis is usually performed with multiple tools for one digital evidence, and if the analysis results differ from one tool to the other, it is difficult to aggregate the analysis results. Therefore, a standard for processing information centered on the land-to-ground principle is proposed to facilitate sharing and intuitively identifying the analysis results of digital evidence collected. It also proposes a way to use it as an indicator to verify the reliability of an analysis tool by comparing the performance of a digital forensics analysis tool.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.4
• /
• pp.471-478
• /
• 2014

Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.

• Journal of Ginseng Research
• /
• v.42 no.3
• /
• pp.343-351
• /
• 2018

Background: Red ginseng is a popularly used traditional medicine with antiaging effects in Asian countries. The present study aimed to explore the changes in protein expression underlying the mechanisms of life span extension and antiaging caused by red ginseng extract (RGE) in Drosophila melanogaster. Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods. Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster. Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.