• 한국수산과학회지
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• 제53권1호
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• pp.123-131
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• 2020

In this study, we investigated the protective effects of a Neutrase enzymatic hydrolysate derived from Korea pen shell Atrina pectinata (APN) against hydrogen peroxide (H₂O₂)-induced oxidative damage in hepatocytes. First, we confirmed that APN has antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS⁺) and H₂O₂ and increasing oxygen radical absorbance capacity (ORAC) value. Also, the treatment of APN increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H₂O₂-stimulated hepatocytes. In addition, APN decreased the sub-G₁ DNA contents and the apoptotic body formation increased by H₂O₂ stimulation. Moreover, APN modulated the protein expression of apoptosis related molecules (Bcl-2, Bax and p53) by suppressing the activation of nuclear factor NFkB and ERK/p38 signaling in H₂O₂-stimulated hepatocytes. Furthermore, APN led to the activation of Nrf2/HO-1signaling known as antioxidant systems. These results suggest APN protects hepatocytes against oxidative damages caused by H₂O₂ stimulation.

• Journal of Radiation Protection and Research
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• 제38권4호
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• pp.166-171
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• 2013

While high-dose ionizing radiation results in long term cellular cytotoxicity, chronic low-dose (<0.2 Gy) of X- or ${\gamma}$-ray irradiation can be beneficial to living organisms by inducing radiation hormesis, stimulating immune function, and adaptive responses. During chronic low-dose-rate radiation (LDR) exposure, whole body of mice is exposed to radiation, however, it remains unclear if LDR causes changes in gene expression of the whole brain. Therefore, we aim to investigate expressed genes (EGs) and signaling pathways specifically regulated by LDR-irradiation ($^{137}Cs$, a cumulative dose of 1.7 Gy for total 100 days) in the whole brain. Using microarray analysis of whole brain RNA extracts harvested from ICR and AKR/J mice after LDR-irradiation, we discovered that two mice strains displayed distinct gene regulation patterns upon LDR-irradiation. In ICR mice, genes involved in ion transport, transition metal ion transport, and developmental cell growth were turned on while, in AKR/J mice, genes involved in sensory perception, cognition, olfactory transduction, G-protein coupled receptor pathways, inflammatory response, proteolysis, and base excision repair were found to be affected by LDR. We validated LDR-sensitive EGs by qPCR and confirmed specific upregulation of S100a7a, Olfr624, and Gm4868 genes in AKR/J mice whole brain. Therefore, our data provide the first report of genetic changes regulated by LDR in the mouse whole brain, which may affect several aspects of brain function.

• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.379-382
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• 2018

Newcastle disease virus (NDV) 감염된 baby hamster kidney 세포에서 Syncytium (합포체) 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase에 의해 일어난다. HAU 값은 추출물의 농도가 25과 $3.2{\mu}g/mL$ 사이에서는 현저하게 감소하였으나, $25{\mu}g/mL$ 농도에서는 NDV 감염된 HAD (%)는 광범위한 흡착능의 감소를 나타났으나 바이러스 당단백질의 세포내 생합성은 저해되지 않았다. 그러므로 오배자 추출물은 바이러스 당단백질의 세포막으로의 수송과 함께 합포체 형성을 저해하여 항바이러스 활성을 갖는 것으로 결론된다. 또한 오배자 추출물의 저해활성을 조사한 결과 ${\alpha}-glucosidase$에 대한 추출물의 $IC_{50}$은 $12.5{\mu}g/mL$이었으며, ${\beta}-glucosidase$, ${\alpha}-glucosidase$, ${\beta}-mannosidase$에 대한 오배자 추출물의 $IC_{50}$은 각각 26, 36, $50{\mu}g/mL$로 나타나 ${\beta}-type$ glycosidases 보다 ${\alpha}-type$ glycosidase에 대한 효소활성 저해능이 우수하였다. 따라서 $IC_{50}$ 농도에서는 세포내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 판단되었으며 향후 항바이러스 관련 작용기작의 연구가 필요하다고 사료된다.

• 문화기술의 융합
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• 제5권2호
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• pp.389-395
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• 2019

본 연구는 인체에 유익한 효과가 있고 부작용 없이 장기간 안전하게 사용할 수 있으며 간보호 및 간기능 개선에 탁월한 효과가 있는지 양배추출물의 간보호 효과에 대하여 알아보았다. 약물(APAP)에 의해 유도된 간손상 동물모델에서 수행된 실험에서, 양배추 추출물은 간손상 마커인 AST 및 ALT를 효과적으로 감소시킴으로써 간 보호 및 간 기능 개선 효과를 나타냄을 알 수 있었다. 양배추 추출물은 간질환의 예방 또는 치료용 약학적 추출물로서 유효하다는 것이다. 특히, 양배추 추출물은 간손상에 수반되는 급성 염증 반응에 관여하는 염증 매개체 iNOS 및 COX-2 및 전염증성 사이토카인 $IL-1{\beta}$의 발현을 감소시킴으로써 간염 염증을 치료하는데 효과적이었다. 그 결과, $100{\sim}150^{\circ}C$에서 열처리 한 배추 추출물은 생배추 추출물에 비해 간기능 개선 효과 또는 항염증 효과가 우수 하였다.

• 한국의상디자인학회지
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• 제22권4호
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• pp.115-125
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• 2020

Rider jackets were once reserved for military uniforms but have become an important styling item in recent fashion trends. The design characteristics of the rider jacket are rooted in symbolism, and the image associated with a rider jacket is in line with the challenging of authority and being a member of the rebellious youth subculture. Usually, young people with anti-social tendencies wore jackets, and some styles were used as a medium to express their emotional homogeneity, and they received favorable responses as the items represented them. The design characteristics of a rider jacket can be largely divided into resistance against the older generation, violence, challenging cultural norms, sexuality, and resistance, as well as embodying violent characteristics, including strength, courage. and male chauvinism. The reason for the development of these challenging characteristics are disparagement and anger of the lower class, who were excluded from mainstream society. Rider jackets can be viewed negatively due to the kind of message it is conveying against mainstream society. Among the sexual features were leather pants, short-length leather rider jackets, glossy metal accessories, and belt buckles, which also highlighted gay and decadent images that came to be associated with the jackets. The drapery created various kinds of wrinkles according to the way of dressing, and it had beautiful expressiveness while serving to express the body more beautifully. Drapery can be classified according to the aesthetic characteristics or expression techniques, and if the type of drapery is classified according to the morphological characteristics, it can be classified into variable and fixed structures, depending on whether the part to which the drapery is applied is fluid or not. In other words, it depends on the dressing method or the intention, and if the drapery technique is directly applied to the garment or is attached to the form. This fashion design proposal may have the greatest significance in that it sought to propose a new style incorporating a drapery technique with a strong feminine image to a rider jacket, which traditionally was associated with a masculine image.

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.835-843
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• 2022

Deinococcus radiodurans is an extremophilic bacterium that can thrive in harsh environments. This property can be attributed to its unique metabolites that possess strong antioxidants and other pharmacological properties. To determine the potential of D. radiodurans R1 lysate (DeinoLys) as a pharmacological candidate for inflammatory bowel disease (IBD), we investigated the antiinflammatory activity of DeinoLys in bone marrow-derived dendritic cells (BMDCs) and a colitis mice model. Lipopolysaccharide (LPS)-stimulated BMDCs treated with DeinoLys exhibited alterations in their phenotypic and functional properties by changing into tolerogenic DCs, including strongly inhibited proinflammatory cytokines (TNF-α and IL-12p70) and surface molecule expression and activated DC-induced T cell proliferation/activation with high IL-10 production. These phenotypic and functional changes in BMDCs induced by DeinoLys in the presence of LPS were abrogated by IL-10 neutralization. Furthermore, oral administration of DeinoLys significantly reduced clinical symptoms against dextran sulfate sodium-induced colitis, including body weight loss, disease activity index, histological severity in colon tissue, and lower myeloperoxidase level in mice. Our results establish DeinoLys as a potential anti-inflammatory candidate for IBD therapy.

• 한국식생활문화학회지
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• 제24권2호
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• pp.219-223
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• 2009

We investigated the effect of Takju lees extract on blood glucose levels in the db/db mice (a murine model of type 2 diabetes mellitus). We fed 40 male db/db mice a control diet (G0, AIN93G) and experimental diets containing 1% (G1), 2% (G2), or 4% (G4) Takju lees extract for 4 weeks. We found no difference in food intake and body weight gain among the animal groups. In the G1 and G2 groups, plasma glucose levels decreased significantly between Days 10 and 21 compared with the G0 group. However, we found no difference in plasma glucose levels between groups G4 and G0. The change in insulin concentrations was not significant among these animal groups, and we found no significant difference in glucose transporter 4 (GLUT4) expression in the soleus muscle. These results suggest that the Takju lees extract has a beneficial effect in animals with type 2 diabetes.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.495-505
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• 2024

Gromwell (Lithospermum erythrorhizon, LE) can mitigate obesity-induced skeletal muscle atrophy in C2C12 myotubes and high-fat diet (HFD)-induced obese mice. The purpose of this study was to investigate the anti-skeletal muscle atrophy effects of LE and the underlying molecular mechanism. C2C12 myotubes were pretreated with LE or shikonin, and active component of LE, for 24 h and then treated with 500 μM palmitic acid (PA) for an additional 24 h. Additionally, mice were fed a HFD for 8 weeks to induced obesity, and then fed either the same diet or a version containing 0.25% LE for 10 weeks. LE attenuated PA-induced myotubes atrophy in differentiated C2C12 myotubes. The supplementation of LE to obese mice significantly increased skeletal muscle weight, lean body mass, muscle strength, and exercise performance compared with those in the HFD group. LE supplementation not only suppressed obesity-induced skeletal muscle lipid accumulation, but also downregulated TNF-α and atrophic genes. LE increased protein synthesis in the skeletal muscle via the mTOR pathway. We observed LE induced increase of mitochondrial biogenesis and upregulation of oxidative phosphorylation related genes in the skeletal muscles. Furthermore, LE increased the expression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha and the phosphorylation of adenosine monophosphate-activated protein kinase. Collectively, LE may be useful in ameliorating the detrimental effects of obesity-induced skeletal muscle atrophy through the increase of protein synthesis and mitochondrial biogenesis of skeletal muscle.

• 한국콘텐츠학회논문지
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• 제12권8호
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• pp.429-437
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• 2012

본 연구는 신체표현을 활용한 색깔과 화음, 다이내믹을 연결하여 일어나는 변화에 목적을 두고 있다. 본 연구를 통하여 영상미디어 음악치료 프로그램 모형개발을 위한 기초 자료 수집을 위해 심리적으로 화음과 다이내믹이 색깔에 변화를 준다는 것을 탐색하였다. 영상매체에서 음악은 비언어적 의사소통으로 많은 분야에서 사용된다. 특히 음과 색깔 관계의 심리적 변화를 통하여 가상과 현실의 갭을 줄이는 데에 가장 효과적인 도구이며 사용자는 메시지와 의미에 대해 우리의 감성을 유도한다. GIM (Method of Guided Imagery and Music)은 인간의 내면세계를 탐색하고 음악에 유도된 다양한 심상 경험을 통해 상징적 의미와 통찰로 자신을 이해하고 문제를 해결해가도록 하는 음악치료방법으로 인간의 내적세계를 광범위하게 다룬다. 본 연구를 통하여 영상미디어라는 도구를 기존의 음악치료에서 사용되어져 왔던 수동적이고 소극적인 음악 감상 활용방법에서 탈피하고 직접 몸으로 체험하는 능동적이고 적극적인 음악치료 프로그램 모형을 적용시켜 나아가 오늘날 급격히 변화하는 청소년기의 사회적응문제에서 긍정적 효과를 줄 수 있는 음악치료 프로그램개발 모형을 위한 기초 자료로 삼고자 한다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7831-7834
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• 2014

Objective: To explore the influence of different ways of blood transfusion on the expression levels of interleukins (IL) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) inperi-operative patients with esophageal cancer. Materials and Methods: A total of 80 patients with esophageal cancer who underwent radical operations were selected as study patients and randomly divided into an observation group (treated with autologous blood transfusion) and control group (with homologous blood transfusion). Changes of intra-operative indexes and peri-operative blood indexes, from hemoglobin (Hb) and hematocrit value (Hct), to levels of inflammatory factors like interleukins-6 (IL-6), IL-8, IL-10 and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were compared. Results: Operations for patients in both groups were successfully conducted, and no significant differences in mean surgical duration and intra-operative hemorrhage volume, fluid infusion volume and blood transfusion volume were detected (p>0.05). Compared with values before surgery, Hb and Hct levels decreased significantly while white blood cell count (WBC) increased 1, 5 and 7 d after operation (p<0.05, p<0.01). In addition, WBC was apparently higher in observation group than in control group 5 and 7 d after operation (p<0.01). Compared with before surgery, in the observation group, levels of IL-6, IL-8 and IL-10 had no significant differences after operation (P>0.05), but TNF-${\alpha}$ level increased y (p<0.01), whereas in control group, IL-6 level had no significant difference (p>0.05), IL-8 level decreased obviously (p<0.05), IL-10 level increased markedly first and then decreased gradually as time passed but its level remained elevated (p<0.01), and TNF-${\alpha}$ level increased first and then decreased, and there was no significant difference 7 d after operation (p>0.05). Conclusions: Decreased IL-8 and increased IL-10 levels are two important reasons for immunosuppression after homologous blood transfusion, whereas autologous blood transfusion can alleviate this while increasing the TNF-${\alpha}$ level, which also has potential to improve anti-tumor immunity in the human body.