• Environmental Analysis Health and Toxicology
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• v.18 no.4
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• pp.255-269
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• 2003

The objective of this study was to estimate human exposure to benzo (a)pyrene through multimedia/multi-pathway exposure scenario. The human exposure scenario for benzo(a)pyrene was consisted of 12 multiple exposure pathways, and the multipathway human exposure model based on this scenario constituted. In this study, the multipathway human exposure model was used to estimate the concentrations in the exposure contact media, human intake factors and lifetime average daily dose (LAD $D_{model}$) of benzo(a)pyrene in the environment. Sensitivity analysis was performed to identify the important parameters and Monte-Carlo simulation was undertaken to examine the uncertainty of the model. The total LAD $D_{model}$ was estimated to be 5.52${\times}$10$^{-7}$ mg/kg-day (2.06${\times}$10$^{-7}$ -8.65${\times}$10$^{-7}$ mg/kg-day) using the multipathway human exposure model. The inhalation dose accounted for 78% of the total LADD, whereas ingestion and dermal contact intake accounted for 20.2% and 1.8% of the total exposure, respectively. Based on the sensitivity analysis, the most significant contributing input parameter was benzo (a)pyrene concentration of ambient air. Consequently, exposure via inhalation in outdoor/indoor air was the highest compared with the exposure via other medium/pathways.

• Journal of Soil and Groundwater Environment
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• v.25 no.3
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• pp.65-73
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• 2020

Exposure assessment methodology for outdoor air inhalation pathways (i.e., inhalation of volatile compounds and fugitive dust in outdoor air) was investigated. Default values of several parameters currently used in Korea (e.g., Q/C; inverse value of concentration per unit flux, and frs; soil fraction in PM10) may not be suitable and lack site-specificity, as they have been adopted from the risk assessment guidance of the United States or the Netherlands. Such limitation can be addressed to a certain degree by incorporating the volatilization factor (VF) and the particulate emission factor (PEF) with Box model. This approach was applied to an exposure assessment of a site contaminated with petroleum hydrocarbons in Korea. The result indicated that the suggested methodology led to more accurate site-specific exposure assessment for outdoor inhalation pathways. Further work to establish methodology to determine site-specific Q/C values in Korea needs to be done to secure the reliability of the exposure assessment for outdoor air inhalation pathways.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.10a
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• pp.58-69
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• 2003

Soil contamination in the United States is managed using a risk-based decision making process. In other words, we don't ask, 'how much soil contamination can be cleaned up\ulcorner' Instead we ask, 'how much contamination can be safely left in place\ulcorner' The determination of 'safe' levels of contamination is based upon the potential for exposure and the toxicity of the contaminants of concern in soil. Potential for exposure is determined by evaluating potential exposure pathways from source to receptor given current or reasonably anticipated land use. Soil cleanup goals are then calculated for any complete exposure pathways based upon toxicity and the route of exposure. In some cases, institutional or engineering controls are also used to limit the potential for exposure. In order to prevent a continuous degradation of environmental quality, risk-based cleanup approaches must be combined with strong contamination prevention programs. In addition, alternative risk management approaches should be incorporated into an overall risk reduction strategy.erall risk reduction strategy.

• Advances in environmental research
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• v.5 no.2
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• pp.79-94
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• 2016

In order to evaluate the contamination and health risk, levels of six hazardous elements i.e., Cr, Ni, Cu, As, Cd and Pb in soils of 12 different land-uses were measured. The average concentration of Cu, Cr, Ni, Pb, As and Cd in soils were 267, 239, 206, 195, 58 and 16 mg/kg, respectively. Levels of each metal exceeded the environmental action level for soils, which could pose significant risk to human. The metal concentrations were subsequently used to establish hazard indices (for adults and children) where the 5th and 95th percentile values were used to derive the hazard index through different exposure pathways (ingestion, dermal contact and inhalation). Considering the total exposure through each of the three pathways, the hazard index elucidates that there was a potency of non-cancer risk at most of the sites for both the adults and children. The findings of this study suggested that different land-use soils were severely contaminated with hazardous elements and attention is needed on the potential health risks to the exposed inhabitants.

• Particle and aerosol research
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• v.20 no.3
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• pp.79-94
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• 2024

Exposure to ambient particulate matter (PM) 2.5 can impair alveolar macrophages (AMs) functions and induce pathological conditions. This study explores the impact of PM2.5 exposure on AMs, focusing on gene expression changes and functional alterations. Murine AMs cell line MH-S were exposed to PM2.5 at a concentration of 100 ㎍/ml for durations of 3 and 48 hours. After 3 hours of exposure, genes involved in inflammatory and immune responses were activated, including TNF and B-cell receptor signaling pathways. In contrast, 48 hours of exposure significantly altered genes related to cell proliferation, apoptosis, and immune processes, with PI3K-Akt and cytokine-cytokine receptor interaction pathways. PM2.5 initially triggers immune responses; however, prolonged exposure over 48 hours impairs AM functions risk of chronic inflammation and cancer. This research provides foundational data for therapeutic approaches related to PM2.5 exposure.

• Toxicological Research
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• v.28 no.1
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• pp.19-24
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• 2012

In the present study, toxicity of silver nanoparticles (AgNPs) was investigated in the nematode, Caenohabditis elegans focusing on the upstream signaling pathway responsible for regulating oxidative stress, such as mitogen-activated protein kinase (MAPK) cascades. Formation of reactive oxygen species (ROS) was observed in AgNPs exposed C.elegans, suggesting oxidative stress as an important mechanism in the toxicity of AgNPs towards C. elegans. Expression of genes in MAPK signaling pathways increased by AgNPs exposure in less than 2-fold compared to the control in wildtype C.elegans, however, those were increased dramatically in sod-3 (gk235) mutant after 48 h exposure of AgNPs (i.e. 4-fold for jnk-1 and mpk-2; 6-fold for nsy-1, sek-1, and pmk-1, and 10-fold for jkk-1). These results on the expression of oxidative stress response genes suggest that sod-3 gene expression appears to be dependent on p38 MAPK activation. The high expressions of the pmk-1 gene 48 h exposure to AgNPs in the sod-3 (gk235) mutant can also be interpreted as compensatory mechanisms in the absence of important stress response genes. Overall results suggest that MAPK-based integrated stress signaling network seems to be involved in defense to AgNPs exposure in C.elegans.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.10a
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• pp.176-177
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• 2005

• Journal of Radiation Protection and Research
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• v.19 no.3
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• pp.209-221
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• 1994

In the case of a severe accident of a nuclear power plant, the whole body dose and the relative importance of the radionuclides during the lifetime of an exposed person were estimated for each exposure pathway with distances from the release point. The external exposure pathways due to immersion of radioactive cloud and deposition of radioactive materials on the ground, and the internal exposure pathways due to inhalation and ingestion of contaminated foodstuffs were considered. The effects due to the ingestion of contaminated foodstuffs were estimated considering the variation of radioactive concentration in the foodstuffs according to deposition time and elapsed time after deposition using a dynamic ingestion pathway model applicable to Korean environment, named 'KORFOOD'. As the results up to 80 km from the release point, the effects due to ingestion of contaminated foodstuffs showed the highest contribution to total exposure dose. The contribution of I isotopes was the highest in the case of the external dose due to immersion of radioactive cloud and internal dose due to inhalation. The contribution of Cs isotopes was highest in the case of the external dose due to deposition of radioactive materials on the ground. In the case of the internal dose due to ingestion of contaminated foodstuffs, Cs deposition in summer and Sr deposition in winter, respectively, were the most dominant radionuclide to whole body.

• Journal of Soil and Groundwater Environment
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• v.16 no.2
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• pp.12-29
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• 2011

Risk-based screening levels (RBSLs) of some pollutants for residential adults were derived with risk assessment tools developed by United States Environmental Protection Agency (USEPA), American Society for Testing and Materials (ASTM), and Korea Ministry of Environment (KMOE) and compared each other. To make the comparison simple, ingestion of soil, dermal contact with soil, outdoor inhalation of vapors, indoor inhalation of vapors, and inhalation of soil particulates were chosen as exposure pathways. The results showed that the derived RBSLs varied for every exposure pathway. For direct exposure pathways (i.e., ingestion of soil and dermal contact with soil), the derived RBSLs varied mainly due to the different default values for exposure factors and toxicity data. When identical default values for the parameters were used, the same RBSLs could be derived regardless of the assessment tools used. For inhalation of vapors and inhalation of soil particulates, however, different analysis methods for cross-media transfer rates were used and different assumptions were established for each tool, identical RBSLs could not be obtained even if the same default values for exposure factors were used. Especially for inhalation of soil particulates pathway, screening level derived using KMOE approach (most conservative) was approximately 5000~10000 times lower than the screening level derived using ASTM approach (least conservative). Our results suggest that, when deriving RBSL using a specific tool, it is a prerequisite to technically review the analysis methods for cross-media transfer rates as well as to understand how the assessment tool derives the default values for exposure factors.

• Molecular & Cellular Toxicology
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• v.4 no.4
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• pp.267-277
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• 2008

Benzene and ethylbenzene (BE), the volatile organic compounds (VOCs) are common constituents of cleaning and degreasing agents, paints, pesticides, personal care products, gasoline and solvents. VOCs are evaporated at room temperature and most of them exhibit acute and chronic toxicity to human. Chronic exposure of benzene is responsible for myeloid leukemia and also ethylbenzene is also recognized as a possible carcinogen. To evaluate the BE effect on human, whole human genome 35 K oligonucleotide microarray were screened for the identification of the differential expression profiling. We identified 280 up-regulated and 201 down-regulated genes changed by more than 1.5 fold by BE exposure. Functional analysis was carried out by using DAVID bioinformatics software. Clustering of these differentially expressed genes were associated with immune response, cytokine-cytokine receptor interaction, toll-like signaling pathway, small cell lung cancer, immune response, apoptosis, p53 signaling pathway and MAPKKK cascade possibly constituting alternative or subordinate pathways of hematotoxicity and immune toxicity. Gene ontology analysis methods including biological process, cellular components, molecular function and KEGG pathway thus provide a fundamental basis of the molecular pathways through BEs exposure in human lymphoma cells. This may provides a valuable information to do further analysis to explore the mechanism of BE induced hematotoxicity.