• BMB Reports
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• v.46 no.2
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• pp.92-96
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• 2013

The breast cancer susceptibility gene BRCA1 encodes a nuclear protein, which functions as a tumor suppressor and is involved in gene transcription and DNA repair processes. Many families with inherited breast and ovarian cancers have mutations in the BRCA1 gene. However, only a few studies have reported on the mechanism underlying the regulation of BRCA1 expression in humans. In this study, we investigated the transcriptional regulation of BRCA1 in HeLa cells treated with etoposide. We found that three Egr-1-binding sequences (EBSs) were located at -1031, -1005, and -385 within the enhancer region of the BRCA1 gene. Forced expression of Egr-1 stimulated the BRCA1 promoter activity. EMSA data showed that Egr-1 bound directly to the EBS within the BRCA1 gene. Knockdown of Egr-1 through the expression of a small hairpin RNA (shRNA) attenuated etoposide-induced BRCA1 promoter activity. We conclude that Egr-1 targets the BRCA1 gene in HeLa cells exposed to etoposide.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.401-401
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• 2014

In this study, graphene, the most attractive material today, has been applied to the wavelength-modulated surface plasmon resonance (SPR) sensor. The optical fiber sensor technology is the most fascinating topic because of its several benefits. In addition to this, the SPR phenomenon enables the detection of biomaterials to be label-free, highly sensitive, and accurate. Therefore, the optical fiber SPR sensor has powerful advantages to detect biomaterials. Meanwhile, Graphene shows superior mechanical, electrical, and optical characteristics, so that it has tremendous potential to be applied to any applications. Especially, grapheme has tighter confinement plasmon and relatively long propagation distances, so that it can enhance the light-matter interactions (F. H. L. Koppens, et al., Nano Lett., 2011). Accordingly, we coated graphene on the optical fiber probe which we fabricated to compose the wavelength-modulated SPR sensor (Figure 1.). The graphene film was synthesized via thermal chemical vapor deposition (CVD) process. Synthesized graphene was transferred on the core exposed region of fiber optic by lift-off method. Detected analytes were biotinylated double cross-over DNA structure (DXB) and Streptavidin (SA) as the ligand-receptor binding model. The preliminary results showed the SPR signal shifts for the DXB and SA binding rather than the concentration change.

• Proceedings of the Materials Research Society of Korea Conference
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• 2010.05a
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• pp.24.1-24.1
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• 2010

In practical operation, the exposed surfaces may get dirty thus degrade the performance of devices. So the combination of self cleaning and antireflection is very desirable for use in outdoor photovoltaic and displaying devices, self cleaning windows and car windshields. For the purpose of self cleaning, the surface needs to be either superhydrophobic or superhydrophilic. However, in practice AR in the visible region and self cleaning are a pair of competitive properties. To satisfy the requirements for superhydrophobic or superhydrophilic surfaces, high surface roughness is required. But it usually cause severely light scattering. Photo-responsive coatings (TiO2, ZnO etc.) can lead to superhydrophilic. However, the refractive indices are high. Thus for porous structure, controlling pore size in the underwavelength scale to reduce the light scattering is very crucial for highly transparent and self cleaning antireflection coating. Herein, we demonstrate a simple method to make high performance broadband antireflection layer on the glass surface, by "carving" the surface by hot alkali solution. Etched glass has superhydrophilic surface. By chemical modification, it turns to superhydrophobic. Enhanced transparency (up to 97%) in a broad wavelength range was obtained by short time etching. Also antifogging effect has been demonstrated, which may offer advantage for devices working at high humidity environment or underwater. Compositional dependence of the properties was observed by comparing three different commercially available glasses.

• Applied Chemistry for Engineering
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• v.1 no.2
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• pp.116-123
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• 1990

Synthesis and characterization of photosensitive orthonaphthoquinonediazide-sulfonyl derivatives were studied. These photoactive compounds underwent a UV induced transformation to the base-soluble photoproduct. The photoresists were prepared using these photoactive compounds with low molecular weight m-cresol novolacs as matrix resin. And photosensitive characteristics of the photoresists were studied. 3, 4, 5-Trihydroxy-benzophenone with bulky resonance structure increased the sensitivity and the solubility rate of the exposed region. The mixture of PAC and matrix resin having 3:8 weight ratio had the moderate rate of dissolution in the developer. The photoresist using these conditions showed the best snsitivity and contrast under the fixed conditions.

• Journal of muscle and joint health
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• v.21 no.3
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• pp.246-254
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• 2014

Purpose: This study aims to provide baseline data on children's health care and household safety management in one region and to support implementation of customized visiting health care services for children. Methods: 51 children and their caregivers from socially vulnerable group and 69 children and their caregivers from general group are selected as research participants. Data were collected through home visiting survey by 10 professional nurses. Results: Children from socially vulnerable group are at higher risks of poor health care and safety accidents in households than general group. Rates of taking developmental screening tests, medical check-up remain considerably low in vulnerable social group. Regarding home safety, vulnerable children are more likely to be exposed to unsafe conditions. Conclusion: These results indicate that in order to promote health care and safety conditions for vulnerable children, it is necessary to implement customized visiting health care programs which actually can reach vulnerable households and meet their demands. These findings can be used as baseline data to develop customized visiting health care programs for children.

• Applied Biological Chemistry
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• v.32 no.2
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• pp.126-131
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• 1989

Binding properties of the degraded 59kD phytochrome from etiolated Avena sativa seedlings to liposomes and Cibacron Blue dye were examined. In contrast with the native 124kD and partially degraded 118kD phytochromes, the farred light absorbing(Pfr) forms of the 59kD phytochrome binds to liposomes and Cibacron Blue dye via electrostatic interactions. Results indicate that the 59kD Pfr does not hold a hydrophobic surface which is exposed upon Pr to Pfr phototransformation of the 124 and 118kD phytochromes. Since a relatively extensive hydrophobic region is located in the chromophore bearing domain(59kD) of phytochrome(Hershey et al., Nuc. Acids Res., 13, 8543, 1986), the 55kD tryptic domain from the C-terminus plays an important role on the exposure of the hydrophobic area in the 118 and 124 Pfr to occur.

• Journal of the Korean Wood Science and Technology
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• v.24 no.2
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• pp.55-60
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• 1996

Tripitaka Koreana were made during Coryo Dynasty from 1236 to 1251 A.D. Buddhist scriptures were engraved on 81.340 wooden plates. Some plates were varnished with Rhus lacquer, but most of them were uncoated. Macroscopically, most of the plates appeared intact due to the storage in a well-ventilated wooden house. Because, they were irregularly used for printings with ink, it can be assumed that they were repeatedly exposed to ink-water and drying processes. The present were made to examine the changes of wood cell structures occurred during long-term aging deterioration processes in these dry archaeological wooden plates. Light, scanning and transmission electron microscopes were employed for this study. Wedge-shaped cracks and delamilations were found from the lumen side toward the compound middle lamellae and they progressed toward primary or secondary walls. A large amount of hypae in vessels and the degradation of vessel-ray pit walls by the fungal hyphae were observed. When compared to the recent wood, the birefringence of wood fibers was considerably lower or completly disappeared, suggesting the degradation of crystalline cellulose in these wood samples. The degradation of the cell wall could be also revealed the calculation of crystallinity with X-ray diffraction and the size of crystalline region was estimated.

• Journal of Microbiology
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• v.39 no.4
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• pp.293-299
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• 2001

The nucleocapsid(NP) protein of Newcastle disease virus (NDV) and its derivative (NP$\sub$cfus)containing the myc region and six histidine residues fused to its C-terminus were pcpressed aboundantly in Escherichia coli. The proteins were purified by sucrose gradient centrifugation. Both the NP and NP$\sub$cfus/ proteins self-assem- bled into ring-like particles stacked together to from nucleocapsid-like structure which are heterogeneous in length with a diameter of 20${\pm}$2 nm and central holow of 5${\pm}$1 nm. Only a very small amount of the monomers in the particles was linked by inter-molecular disulfide bonds. Fusion of the C-terminal end to 29 amino acids inclusive of the myc epitope and His tag did not impair ring assembly buy inhibited the formation of the long herringbone structures. Immunogold lableing of the particles with the anti-myc antibody showed that the C-terminus of the NP$\sub$cfus/ protein is exposed on the surface of these ring-like particles.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.2
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• pp.178-185
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• 2005

The synergistic effect of ethylene/propane mixture on soot formation is studied experimentally using a concentric co-flow diffusion burner, which provides the stratified fuel mixture. The soot volume fraction, soot particle diameter, number density and PAH concentrations are measured with various fuel supply configurations and compared to the homogeneously mixed case. When propane is supplied through the inner nozzle, an increase of soot formation is observed. However, when propane is supplied through the outer nozzle, a decrease is observed. The reaction path of PAH's formed from the pyrolysis process of propane is likely to be responsible to the observed differences. When propane is supplied through the outer nozzle, PAH's are formed in the relatively near oxidation region and exposed to the oxidization environment; on the other hand, when propane is supplied through the inner nozzle, PAH's are not likely to be oxidized and thus get involved in soot formation process. The synergistic effect in ethylene/propane diffusion flames is found to be affected not only by the com position of the mixture but also by the way of mixing.

• Nuclear Engineering and Technology
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• v.41 no.10
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• pp.1323-1332
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• 2009

The IHX (Intermediate Heat eXchanger) for a pool-type SFR (Sodium-cooled Fast Reactor) system transfers heat from the primary high temperature sodium to the intermediate cold temperature sodium. The upper structure of the IHX is a coaxial structure designed to form a flow path for both the secondary high temperature and low temperature sodium. The coaxial structure of the IHX consists of a central downcomer and riser for the incoming and outgoing intermediate sodium, respectively. The IHX of a pool-type SFR is supported at the upper surface of the reactor head with an IHX support structure that connects the IHX riser cylinder to the reactor head. The reactor head is generally maintained at the low temperature regime, but the riser cylinder is exposed in the elevated temperature region. The resultant complicated temperature distribution of the co-axial structure including the IHX support structure may induce a severe thermal stress distribution. In this study, the structural feasibility of the current upper support structure concept is investigated through a preliminary stress analysis and an alternative design concept to accommodate the IHTS (Intermediate Heat Transport System) piping expansion loads and severe thermal stress is proposed. Through the structural analysis it is found that the alternative design concept is effective in reducing the thermal stress and acquiring structural integrity.