• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.500-508
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• 2016

This study was conducted to investigate the effects of Momordica charantia saponin (MCS) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. Five levels of MCS supplements (0, 0.01, 0.06, 0.30, 0.60 mg/mL) were tested. The pH, $NH_3-N$, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. The high dose of MCS increased the initial fractional rate of degradation at t-value = 0 ($FRD_0$) and the fractional rate of gas production (k), but decreased the theoretical maximum of gas production ($V_F$) and the half-life ($t_{0.5}$) compared with the control. The $NH_3-N$ concentration reached the lowest concentration with 0.01 mg MCS/mL at 6 h. The MSC inclusion increased (p<0.001) the molar proportion of butyrate, isovalerate at 24 h and 48 h, and the molar proportion of acetate at 24 h, but then decreased (p<0.05) them at 48 h. The molar proportion of valerate was increased (p<0.05) at 24 h. The acetate to propionate ratio (A/P; linear, p<0.01) was increased at 24 h, but reached the least value at the level of 0.30 mg/mL MCS. The MCS inclusion decreased (p<0.05) the molar proportion of propionate at 24 h and then increased it at 48 h. The concentration of total volatile fatty acid was decreased (p<0.001) at 24 h, but reached the greatest concentration at the level of 0.01 mg/mL and the least concentration at the level of 0.60 mg/mL. The relative abundance of Ruminococcus albus was increased at 6 h and 24 h, and the relative abundance of Fibrobacter succinogenes was the lowest (p<0.05) at 0.60 mg/mL at 6 h and 24 h. The relative abundance of Butyrivibrio fibrisolvens and fungus reached the greatest value (p<0.05) at low doses of MCS inclusion and the least value (p<0.05) at 0.60 mg/mL at 24 h. The present results demonstrates that a high level of MCS quickly inhibits in vitro fermentation of maize stover, while MCS at low doses has the ability to modulate the ruminal fermentation pattern by regulating the number of functional rumen microbes including cellulolytic bacteria and fungi populations, and may have potential as a feed additive applied in the diets of ruminants.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.505-507
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• 1994

A total of 526 domestic and experimental animals in Miyagi prefecture, Japan were investigated for fecal carriage of Campylobacter jejuni and Campylobacter coli. C. jejuni was detected in chickens (8.2%), dogs (6.3%), pigs (4.3%), cattle (1.8%) and hamsters (1.4%). C. coli was only detected from pigs (20.7%). Drug susceptibility test was performed on 5 strains of C. jejuni isolated from chickens and 13 strains of C. coli isolated from pigs to tylosin (TS), thianphenicol (TP), carbadox (CDX), chroltetracyclin (CTC), vancomycin (VCM), cefoperazone (CPZ), latamoxef (LMOX), GM were highly effective and CTC, CP and PL were moderately effective against both C. jejuni and C. coli. TS and TPH were moderately effective against C. jejuni; however, they were less effective to C. coli. One strain of C. jejuni against CTC considered to be drug resistant. The results suggest that C. jejuni and C. coli can be controlled by several drugs effectively, although a drug resistant strain exists.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.35-40
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• 2001

The physiological role of brain neuropeptide Y (NPY) in the central regulation of grass intake in sheep was investigated through a continuous intracerebroventricular (ICV) infusion of NPY at a dose of $5{\mu}g/0.2ml/hr$ for 98.5 hours from day 1 to day 5. Sheep (n=5) were fed for 2 hours once a day, and water and 0.5 M NaCl solution were given ad libitum. Feed intake during ICV NPY infusion increased significantly compared to that during ICV artificial cerebrospinal fluid (CSF) infusion. Water and NaCl intake during ICV NPY infusion remained unchanged. Mean arterial blood pressure (MAP) and plasma osmolality during ICV NPY infusion were not significantly different from those during ICV CSF infusion. On the other hand, plasma glucose concentration during ICV NPY infusion increased significantly compared to that during ICV CSF infusion. The results suggest that brain NPY acts as a hunger factor in brain mechanisms controlling feeding to increase grass intake in sheep.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.299-302
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• 2011

During experimental Eimeria infections in chickens, facilities are often contaminated by fecal oocysts known to be highly resistant to both chemical and enzymatic treatments. Thus, studies using experimental Eimeria infections have been limited due to the difficulty of complete elimination of residual oocysts from both cages and facilities. To overcome this limitation, simple, inexpensive, and disposable cages were constructed from cardboard boxes and tested during experimental Eimeria maxima infections. The cages were used in animal rooms with only a 1.7% evidence of coccidia contamination between adjacent cages. No significant differences in fecal oocyst output and body weight gain were noted between animals housed in disposable cages and animals housed in wire control cages. This cage design is a useful means for preventing oocyst contamination during experimental conditions, suggesting that this disposable cage design could be used for other avian infectious disease studies.

• 대한수의학회지
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• 제41권4호
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• pp.579-582
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• 2001

SHR 랫드 4-21 개월령 colony에서 혈청 및 조직학적으로 정기적인 health monitoring을 실시하던 중 ELISA test에서 21 개체 중 12 개체에서 Mycoplasma pulmonis에 대한 항체가 검출되었다. Mycoplasma 양성 개체군의 부검 소견에서 기관지, 세기관지 주변 림프절의 증식과 전형적인 폐 기관지의 병변 소견을 보였다. 혈청학적 검사와 조직학적 소견에서 SHR 랫드 군의 자연적인 Mycoplasm 감염 예로 진단되어 보고하는 바이다.

• 농업과학연구
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• 제47권2호
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• pp.275-282
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• 2020

Two experiments were conducted to examine the effects of the difference in energy concentration in diets on performance and nutrient digestibility of growing-finishing pigs. The experimental diets were as follows: 1) a normal energy level corn-soybean meal-based diet (CON) and 2) high-energy diet compared with the CON (HE). Pigs had free access to their feed and water ad labium for 6 weeks during each experimental period. In experiment 1, 60 growing pigs (initial body weight [BW] of 23.85 kg) were randomly allotted to 2 treatment groups with 5 replications (6 pigs·pen^-1). In experiment 2, 48 finishing pigs (initial BW = 65.13 kg) were randomly assigned to 2 treatment groups with 6 replications (4 pigs·pen^-1). The growth performance was measured at the beginning and end of each period. The apparent total tract digestibility (ATTD) was determined by chromium oxide as an indigestible marker during the last 7 days of each experiment. In experiment 1, the dietary treatments did not affect the growth performance and ATTD of energy and nutrients. In experiment 2, no differences in growth performance were observed for pigs fed CON and HE throughout the experimental period. Additionally, dietary treatments did not affect the ATTD. In conclusion, the high energy content in diets for the growing-finishing period had no effect on the growth performance or digestibility, indicating that a wide range of energy content changes in diets would be required to affect the performance and digestibility of grower-finisher pigs. It is also necessary to understand the characteristics of components used to adjust the dietary energy concentration.

• 대한수의학회지
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• 제22권1호
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• pp.59-62
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• 1982

This paper dealt with histopathological observations of the nephroblastoma in the pregnant pig died on 8 days before paturation day. The results summarized as follows: Authors considered that in necropsy finding the pregnant pig died of circulative and respiratory disturbance by compression of fetus and renal tumor weighing about 12Kg. In microscopical findings of the renal tumor it was composed of well differentiated tumor cells and connective tissue elements. As differentiation of the neoplastic epithelial cells progressed, tubular and cystic structures, and papillary structures resembled to glomeruli appeared. This tumor was regarded as nephrohlastoma.

• 대한수의학회지
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• 제39권4호
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• pp.698-701
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• 1999

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1400-1404
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• 2001

$Holstein\;{\times}\;indigenous$ multiparous dairy cows were offered diets with increasing roughage neutral detergent fiber (NDF) contents to determine the effects on intake, milk yield and compositions. Roughage NDF contents were 15, 18, 21 and 24% dry matter (DM), and concentrate NDF content was 10% DM. Experimental treatments were isonitrogenous and isocaloric diets. Maximum and minimum temperature humidity index during the experimental period were 85.9 and 76, respectively. Intakes of DM, crude protein and net energy, 4% fat corrected milk, milk protein and average daily gain decreased with increasing roughage NDF contents (p<0.05). Intakes of ether extract (p<0.01) and dietary NDF (p>0.05) and milk fat (p<0.01) increased with increasing roughage NDF contents. The results support the conclusion that higher DM intake, optimal milk yield and compositions can be maintained with lower roughage NDF diets for dairy cows under tropical conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1395-1399
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• 2004

The objective of this study was to evaluate the effect of dietary fat inclusion on nutrient intake, body weight, milk production, return to estrus, pregnancy and lambing of winter-lambing, postpartum Awassi ewes. Thirty multiparous, winter-lambing Awassi ewes (body weight=51${\pm}$7.0 kg) were randomly assigned to three dietary treatments (n=10) for 62 days using a completely randomized design. Experimental diets were isonitrogenous, and were formulated to contain 0 (CON), 2.5 (MF), and 5% (HF) added fat, and 33% of the dietary crude protein (CP) as undegradable intake protein (UIP). On day 26 postpartum (day 0=parturition), ewes and their lambs were housed in individual pens for 28 days. Feed offered and refused was recorded daily. At the end of this period, ewes and their lambs within each treatment were combined into one group and fed their respective diet ad libitum. One fertile Awassi ram fitted with a marking harness was allowed with each group for 34 days. No significant (p>0.05) differences in dry matter intake, organic matter intake, and crude protein intake were observed for ewes fed the three experimental diets. No difference was observed in metabolizable energy intake (MEI) for ewes fed the CON and the MF diets (average 8.3 Mcal/d) diet. However, ewes fed the HF diet had greater(p<0.05) MEI compared with the rest of the treatments. Ewe body weights increased throughout the study, unaffected by the experimental diets. No significant differences in milk production were found among ewes fed the three experimental diets. No significant differences were observed in pregnancy rate (6/10, 5/10, 6/10 for CON, MF and HF diets, respectively), lambing rate and the number of lambs per ewe among the three treatments. postpartum reproductive performance of well-fed, winter-lambing Awassi ewes.