• 제목/요약/키워드: experimental animal

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Effects of Resveratrol and Essential Oils on Growth Performance, Immunity, Digestibility and Fecal Microbial Shedding in Challenged Piglets

  • Ahmed, S.T.;Hossain, M.E.;Kim, G.M.;Hwang, J.A.;Ji, H.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권5호
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    • pp.683-690
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    • 2013
  • A study was conducted to evaluate the effects of resveratrol and essential oils from medicinal plants on the growth performance, immunity, digestibility, and fecal microbial shedding of weaned piglets. A total of 48 weaned piglets (8 kg initial weight, 28-d-old) were randomly allotted to four dietary treatments with 3 replications of 4 piglets each. The dietary treatments were NC (negative control; basal diet), PC (positive control; basal diet+0.002% apramycin), T1 (basal diet+0.2% resveratrol), and T2 (basal diet+0.0125% essential oil blend). All piglets were orally challenged with 5 ml culture fluid containing $2.3{\times}10^8$ cfu/ml of Escherichia coli KCTC 2571 and $5.9{\times}10^8$ cfu/ml Salmonella enterica serover Typhimurium. The PC group (p<0.05) showed the highest average daily gain (ADG) and average daily feed intake (ADFI) throughout the experimental period, although feed conversion ratio (FCR) was improved in the T1 group (p>0.05). Serum IgG level was increased in the T1 group, whereas TNF-${\alpha}$ levels was reduced in the supplemented groups compared to control (p<0.05). The PC diet improved the dry matter (DM) digestibility, whereas PC and T2 diets improved nitrogen (N) digestibility compared to NC and T1 diets (p<0.05). Fecal Salmonella and E. coli counts were reduced in all treatment groups compared to control (p<0.05). Fecal Lactobacillus spp. count was increased in the T2 group compared to others (p<0.05). Dietary treatments had no significant effect on fecal Bacillus spp. count throughout the entire experimental period. Based on these results, resveratrol showed strong potential as antibiotic alternatives for reversing the adverse effects of weaning stress on growth performance, immunity and microbial environment in E. coli and Salmonella-challenged piglets.

돼지분뇨 슬러리 액비화시 폭기가 액비특성 및 슬러지 형성에 미치는 영향 (Effect of Aeration on Fertilization and Sludge Accumulation of Pig Slurry)

  • 정광화;;이명규;김중곤;한덕우;곽정훈
    • 한국축산시설환경학회지
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    • 제19권1호
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    • pp.47-54
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    • 2013
  • Two types of reactors were set to investigate the change of characteristics of pig slurry by aeration during fertilization period. One system was equipped with air diffuser to supply oxygen to pig slurry for liquid fertilization, but there was no air diffuser in the other system. Air supply to the experimental systems was regulated by air flow meter. The reactors were set up in the laboratory to protect the pig slurry from external condition such as temperature and humidity changes. Maintaining optimal pH range in the experimental reactors is an important factor for liquid fertilization of pig slurry. In this study, pH ranges of aerobic reactor and anoxic reactor was 7.04~7.19 and 7.34~7.81, respectively. The temperature of aerobic reactors was $2{\sim}3^{\circ}C$ higher then indoor temperature. The amount of sludge accumulated at the bottom layer of non-aerated reactors was 4~5 times more than that of aerated reactors.

Comparison of Forage Production and Nutritional Value of Italian ryegrass, Rye and Whole Crop Barley as Winter Forage Crops in Southern Region of Korea

  • So, Min Jeong;Kim, Hyeon Shup;Kim, Ji Hye;Lee, Sang-Hoon;Lee, Ki-Won;Jung, Jeong Sung;Sung, Kyung-Il;Peng, Jing lun;Park, Hyung Soo
    • 한국초지조사료학회지
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    • 제35권3호
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    • pp.245-250
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    • 2015
  • This study was conducted from October 2014 to May 2015 to explore forage production and feed values of Italian ryegrass, Rye and whole crop barley as winter forage crops in the Southern region of Korea. The experimental location was over 10 points for each species and each sampling point area was $1m^2$ (Width: 1 m ${\times}$ Length: 1 m). Air mean temperature and rainfall in the Southern region of Korea during the experimental period was $6.95{\pm}5.75^{\circ}C$ and $70.45{\pm}54.68mm$, respectively. Fresh forage yield of Italian ryegrass, the most cultivated forage in the Southern region of Korea, was $44.4{\pm}7.0ton/ha$. The percentage of dry matter for whole crop barley was $28.9{\pm}7.0%$. Crude protein (CP) was higher in Italian ryegrass ($10.7{\pm}5.3%$) while total digestible nutrient (TDN) had the highest value in whole crop barley. Crude protein was not significantly different by location. However, the neutral detergent fiber (NDF), acid detergent fiber (ADF) and total digestible nutrient value of forage from Jeonbuk province were higher than in forage from Gyeongnam province.

Effect of Cichorium intybus on production performance, carcass quality and blood lipid profile of hybrid duck

  • Nafiatul, Umami;Eka Rizky Vury, Rahayu;Bambang, Suhartanto;Ali, Agus;Edi, Suryanto;Mohammad Mijanur, Rahman
    • Animal Bioscience
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    • 제36권1호
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    • pp.84-97
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    • 2023
  • Objective: One hundred hybrid male ducks (Mojosari×Alabio) were used to examine the efficacy of chicory supplementation as nutritional feed manipulation on production performance, and blood lipid profile of hybrid ducks. Methods: The ducks were tagged, weighed, and then allotted randomly to one of the four treatment diets using a completely randomized design. The experimental diets were: i) P0 (100% basal diets+0% chicory as control), ii) P1 (95% basal diets+5% chicory), iii) P2 (90% basal diets+10% chicory) and iv) P3 (85% basal diets+15% chicory). For each treatment group, there were 5 replicates of 5 birds each. All experimental diets were isonitrogenous and isocaloric using locally available ingredients. Results: Hybrid ducks with fed diets supplemented fresh chicory (5%, 10%, and 15%) showed increased feed intake and body weight gain, as well as feed conversion ratio to be smaller than those ducks fed diets without chicory supplementation (control). The ducks fed 10% chicory supplementation contained significantly (p<0.05) lower ash and higher organic matter contents of meat than those ducks fed other diets. The ducks fed 15% chicory supplementation showed the lowest crude protein and cholesterol content of meat among the treatment diets. Ducks fed chicory supplementation showed lower (p<0.05) blood cholesterol and triglyceride levels than those ducks fed without chicory supplementation, while dietary interventions had no major (p>0.05) influence on low-density lipoprotein and high-density lipoprotein levels in duck blood. Conclusion: In this study, 10% chicory supplementation showed the best results characterized by an increase in growth performance, carcass quality, small intestinal histomorphology, and lower cholesterol levels of meat.

Effect of Dietary Supplementation with Yeast Cell Suspension (Saccharomyces cerevisiae) on Nutrient Utilisation and Growth Response in Crossbred Calves

  • Singh, Rameshwar;Chaudhary, L.C.;Kamra, D.N.;Pathak, N.N.
    • Asian-Australasian Journal of Animal Sciences
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    • 제11권3호
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    • pp.268-271
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    • 1998
  • Twenty crossbred calves of $88{\pm}5.5kg$ initial live weight and 3-4 month of age were divided into two groups and fed wheat straw and concentrate to support a 500 g daily gain in body weight. Calves in the experimental group (YC) were given a daily dose of 10 ml yeast cell suspension (YC) containing live cells $(5{\times}10^9 cells/ml)$ of Saccharomyces cerevisiae ITCCF 2094. After a growth study of 122 days metabolism trials were conducted. The calves in the YC group recorded a daily weigt gain of $492{\pm}27.8g$ as compared to $476{\pm}20.1g$ in control group. There were no significant differences in feed intake, nutrient digestibility, feed/gain ratio and nitrogen retention between the YC supplemented and control groups.

Fermentation Pattern and Enzymatic Activity in Caecum of Rabbits Fed Processed Neem (Azadirachta indica) Kernel Meal Incorporated Diets

  • Gowda, S.K.;Sastry, V.R.B.;Sahoo, A.;Katiyar, R.C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제10권4호
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    • pp.398-401
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    • 1997
  • A caecal fermentation study was conducted in 30 Angora rabbits equally placed under five whole diets (75 concentrate : 25 roughage) supplemented with processed Neem (Azadirachta indica) kernel meal (NKM ; 2% urea or 1.5% NaOH, W/W), replacing isonitrogenously either at 50 or 100% level of deoiled peanut (Arachis hypogea) meal, for 18 wk period. The total volatile fatty acids level was depressed (p < 0.05) in all the experimental groups (0.02 to 0.04 mEq/g) and ammonia-nitrogen was lowest (p < 0.05; $24.9{\mu}mol/g$) in rabbits fed NaOH treated NKM supplemented diet. Enzyme activity (unit/g) of carboxy methyl-cellulase, ${\alpha}$-amylase, protease and urease exhibited much variation and did not differ significantly. Hence, the results could not confirm a possible adverse effect of feeding NKM on caecal fermentation.

동물 실험을 적용한 국내 간호 연구 동향 분석 (Use of Animals for Nursing Research in Korea)

  • 유해영
    • Journal of Korean Biological Nursing Science
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    • 제17권4호
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    • pp.324-330
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    • 2015
  • Purpose: The purpose of this study was to analyze the trends in Korea of nursing research using animals. Methods: 45 articles were reviewed with descriptive statistics from 2000 to 2015. Results: Nearly 50% of the studies were supported by research funds. Most of the animal species in the studies were rats (81.1%) and mice (15.6%). The major animal models for human disease were cerebrovascular diseases (24.4%), skin problems (17.8%), and nerve injuries (15.5%). Immunohistochemistry (24.3%) was mainly used as an experimental technique. The main intervention and treatments used in the studies were chemical treatments, exercise and surgery. Conclusion: In this review, relevant results were described to identify the trends of nursing research using animal experimentation in Korea. Expansion of various nursing research using animal models can be required for gaining knowledge applicable to the same conditions in humans.

Minipigs as Laboratory Animals: Facility Management and Husbandry

  • Koo, Ok-Jae;Jang, Goo;Lee, Byeong-Chun
    • Reproductive and Developmental Biology
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    • 제36권1호
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    • pp.79-85
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    • 2012
  • Minipigs are regarded as one of the most important laboratory animal in that anatomical and physiological properties are similar to human and their reproduction efficiency is relatively higher compared to other large animal species. Particularly, several diseases that cannot be mimicked in rodent models are successfully occurred or induced in pig models therefore it has been interested in a valuable model for human diseases. Pigs are also 'standard' species in xenotransplantation research. To maximize experimental outcome using minipigs, establishment and management of proper animal facility, right animal husbandry and control of pathogens are very important. In this review, we summarized several international guidelines related with minipigs published by several companies or governments and discuss optimal conditions for providing informative ideas to the researchers who want to use minipigs in their future studies.

Effects of the multimodal intervention program including animal-assisted therapy on depression and self-esteem among university students

  • Kil, Taeyoung
    • Journal of Animal Science and Technology
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    • 제63권6호
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    • pp.1443-1452
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    • 2021
  • This study aimed to investigate the effects of the multimodal group intervention that combined animal-assisted therapy (AAT) and integrated play therapy (IPT) on depression and self-esteem in undergraduate university students. The subjects were 40 students attending animal-related and social welfare departments of universities located in a metropolitan city. The multimodal intervention program was conducted for eight sessions (twice a week, 60 min each) in the experimental group. Data analysis was conducted using the independent sample t-test, ANCOVA, and paired sample t-test for pre- and post-test. Therefore, the multimodal intervention program applying AAT and IPT showed positive effects on depression and self-esteem in university students. Based on these results, this study proposed the operation of multidisciplinary education and practical and policy utilization methods to reduce depression among university students and help improve their self-esteem.

Evaluating sulfoxaflor residues in pig tissues using animal modeling

  • Hyun-Woo, Cho;Kangmin, Seo;Jin Young, Jeong;Ju Lan, Chun;Ki Hyun, Kim
    • Journal of Animal Science and Technology
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    • 제64권5호
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    • pp.911-921
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    • 2022
  • Maximum residue limits (MRL) for pesticides in feed have been set to protect public health and produce safe livestock products. In vivo experiments to establish MRL are essential, as livestock are commonly used to obtain reliable in vivo quantitative information. Here, we aimed to evaluate whether small laboratory animals can replace or reduce monogastric livestock in experiments to quantify pesticide residues in vivo after oral consumption through feed. First, 24 pigs and rats were randomly assigned to four groups and fed 0, 3, 9, or 30 mg/kg of sulfoxaflor. After four weeks, serum, muscle, fat, liver, kidney, and small intestine samples were collected, and sulfoxaflor residues were analyzed using liquid chromatography - tandem mass spectrometry. Sulfoxaflor residues in pig tissues were significantly correlated with those in rat tissues. Model equations were formulated based on the residual sulfoxaflor amount in pig and rat tissues. The calculated and measured sulfoxaflor residues in pigs and rats showed more than 90% similarity. Sulfoxaflor did not affect body weight gain, feed intake, or the feed conversion ratio. Therefore, we concluded that pesticide residue quantification in vivo to establish MRL could be performed using small laboratory animals instead of livestock animals. This would contribute to obtaining in vivo pesticide residue information and reducing large-scale livestock animal experiments.