• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.134-134
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• 2003

Bovine embryos produced by in vitro maturation, feretilization and development was examined for presevation and transfer. The fertilization medium used BO medium with 5 mM/$m\ell$ caffeine and 10$\mu\textrm{g}$/$m\ell$ heparin and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to 1$\times$$10^{6}$ cells/$m\ell$ motile sperm during fertilization in vitro. At 8~10 hrs after insemination, the oocytes were transferred into CR1aa medium and cultured for 7 days. Embryos were preserved by vitrification method for transfer. When the embryos of early, blastocyst and expanded blastocyst stages were frozen-thawed, the proportions of embryos with normal morphology 83.6, 88.1 and 85.2%. (중략)

• Journal of the Korean Society for Precision Engineering
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• v.14 no.8
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• pp.49-56
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• 1997

In recent years, ultrasonic technics has been widely applied to industrial fields and its application range has been expanded as a result of continuous research and development. This paper is concerned with development of post-processor program for ultrasonic test and two-axis automatic ultrasonic system for application of industrial fields. Test results of ultrasonic test post-processor program and two-axis auto- matic ultrasonic system have a good agreement with results of ultrasonic evaluation for defect detection in industrial fields. Therefore we think that the developed ultrasonic test post-processor program and two- axis automatic ultrasonic system in this work is very useful for application of industrial fields.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1149-1154
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• 2010

Liquid chromatography/mass spectrometry using isotope dilution method has been established as a primary method for the measurement of cortisol in human serum. Verification of this method was accomplished by the participation in Consultative Committee for Amount of Substance-Metrology in Chemistry (CCQM) pilot study. Two levels of cortisol certified reference materials were prepared and certified by the established method. They were used as sample materials for the proficiency testing. The expanded uncertainty in the measurement of cortisol in human serum was approximately 1.2% at 95% confidence level. The results of the proficiency testing showed a good precision among the participants, but some bias to the certified values. This means that commercial field laboratories should keep traceability chain to SI unit through available reference measurement procedures and/or available reference materials.

• Structural Engineering and Mechanics
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• v.63 no.6
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• pp.761-769
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• 2017

Accurately extracting the axle distribution information of a passing vehicle from bridge dynamic responses experiences a key and challenging step in non-pavement bridge weigh-in-motion (BWIM). In this article, the wavelet transformation is adopted and the wavelet coefficient curve is used as a substitute for dynamic response. The driving frequency is introduced and expanded to multi-axle vehicle, and the wavelet coefficient curve on specific scale corresponding to the driving frequency is confirmed to contain obvious axle information. On this basis, an automatic method for axle distribution information identification is proposed. The specific wavelet scale can be obtained through iterative computing, and the false peaks due to bridge vibration can be eliminated through cross-correlation analysis of the wavelet coefficients of two measure points. The integrand function that corresponds to the maximum value of the cross-correlation function is used to identify the peaks caused by axles. A numerical application of the proposed axle information identification method is carried out. Numerical results demonstrate that this method acquires precise axle information from the responses of an axle-insensitive structure (e.g., girder) and decreases the requirement of sensitivity structure of BWIM. Finally, an experimental study on a full-scale simply supported bridge is also conducted to verify the effectiveness of this method.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.4
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• pp.728-732
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• 2010

GIS(Gas insulated switching gear) is power equipment with excellent dielectric strength and is economy merit in high confidence and stability. Recently, because equipment of GIS was occurring problem of confidence used for a long time, partial discharge on-line diagnosis systems have been importantly recognized. Partial discharge (PD) detection is an effective means for monitoring and evaluation of dielectric condition of gas insulated system (GIS). The ultra-high-frequency (UHF) PD detection technique can detect and locate the PD sources inside GIS by detecting electromagnetic wave emitted from PD source. Therefore, real-time diagnostic system using UHF detection method has been developed for this application is being expanded gradually. However, the signal of partial discharge occurring in SF6 gas is very weak and susceptible to external noises which mainly consist of PD in air. Thus, it is important to distinguish the PD in SF6 gas more sensitively from the external noises. Unfortunately, these external noise signals and the partial discharge signals have very similar characteristics. Therefore, to solve this problem, we need the signal processing method for distinguish partial discharge signals with external noise signals for improvement of SNR(signal to noise ratio) and sensitivity. In this paper, we proposed internal signal processing method for removing external noise signals with built-in pre.amplifier and frequency conversion circuit.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.2
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• pp.133-141
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• 2011

In this study, different calibration approaches for reduced sulfur compounds (RSCs) were investigated by using thermal desorption coupled with gas chromatography (GC) and pulsed flame photometric detection (PFPD). To evaluate the effects of calibration procedures, gaseous standards of 4 RSCs ($H_2S$, $CH_3SH$, DMS, and DMDS) prepared at 10 ppm level were analyzed at 7 loading injection volumes (40, 60, 80, 100, 160, 240, and 320 ${\mu}L$). The results were then compared with calibration curves made with the Z (zero offset) and N (non-zero offset) method. The concentrations of unknown samples were then quantified by using R (ratio) method in which the slope values are compared between standards and samples. Secondly, in A (average) method, results obtained from a multi-point analysis of unknown samples were also averaged to extract representative values for each sample. Results of both experiments showed that analytical error of low molecular weight components (such as $H_2S$ and $CH_3SH$) was greatly expanded with the Z method. In conclusion, the combined application of N-A method was the more realistic approach to reduce biases in the quantification of RSCs.

• Journal of information and communication convergence engineering
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• v.9 no.3
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• pp.319-324
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• 2011

In computing the optical flow. Horn and Schunck's method which is a representative algorithm is based on differentiation. But it is difficult to estimate the velocity for a large displacement by this algorithm. To cope with this problem multigrid method has been proposed. In this paper, we have proposed a scaled multigrid algorithm which the initial flow for a level is calculated by the summation of the optimally scaled flow and error flow. The optimally scaled flow is the scaled expanded flow of the previous level, which can generate an estimated second image having the least RMS error with respect to the original second image, and the error flow is the flow between the estimated second image (generated by the optimally scaled flow) and the original second image. The flow for this level is then estimated using the original first and second images and the initial flow for that level. From among the various coarsest starting levels of the multigrid algorithm, we select the one that finally gives the best estimated flow. Better results were achieved using our proposed method compared with Horn and Schunck's method and a conventional multigrid algorithm.

• Interaction and multiscale mechanics
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• v.3 no.3
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• pp.213-234
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• 2010

A multiscale method is presented for analysis of thin slab structures in which the microstructures can not be reduced to two-dimensional plane stress models and thus three dimensional treatment of microstructures is necessary. This method is based on the classical asymptotic expansion multiscale approach but with consideration of the special geometric characteristics of the slab structures. This is achieved via a special form of multiscale asymptotic expansion of displacement field. The expanded three dimensional displacement field only exhibits in-plane periodicity and the thickness dimension is in the global scale. Consequently by employing the multiscale asymptotic expansion approach the global macroscopic structural problem and the local microscopic unit cell problem are rationally set up. It is noted that the unit cell is subjected to the in-plane periodic boundary conditions as well as the traction free conditions on the out of plane surfaces of the unit cell. The variational formulation and finite element implementation of the unit cell problem are discussed in details. Thereafter the in-plane material response is systematically characterized via homogenization analysis of the proposed special unit cell problem for different microstructures and the reasoning of the present method is justified. Moreover the present multiscale analysis procedure is illustrated through a plane stress beam example.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.25-30
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• 2000

Residual stresses induced in U-bending and tube-to-tubesheet joining processes of PWR's steam generator row-1 tube were measured by X-ray method and Hole-Drilling Method(HDM). The stresses resulting from the Internal pressure and the temperature gradient in the steam generator were also estimated theoretically. In U-bent lesions, the residual stresses at extrados were induced with compressive stress(-), and its maximum value reached -319 MPa in axial direction at ${\psi}=0^{\circ}$ in position. Maximum tensile residual stress of 170MPa was found to be at the flank side at Position of${\psi}=90^{\circ}$, i.e., at apex region. In tube-to-tubesheet fouling methods, the residual stresses induced by the explosive joint method were found to be lower than that by the mechanical roll method. The gradient of residual stress along the expanded tube was highest at the. transition region, and the residual stress in circumferential direction was found to be higher than the residual stress in axial direction. Hoop stress due to an internal pressure between primary and secondary side was analyzed to be 76 MPa and thermal stress was 45 MPa.

• Journal of Korean Society of Water and Wastewater
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• v.30 no.5
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• pp.491-500
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• 2016

Pressure monitoring is expected to be expanded in a water distribution system according to accelerated development of smart water network management technologies caused by appearances of affordable digital infrastructures like computing, storage and bandwidth. However, the placement of pressure sensors has been determined by engineer's technical decisions since there is no well-defined criteria for deciding a suitable location of pressure sensor. This study presents a placement method of pressure sensors based on the consideration of allowable error in calibrating water network analysis modeling. The proposed method is to find a minimum set of pressure sensors for achieving a reliable management of water transmissions main and increasing the efficiency of their real-time operation. In the case study in Y area's transmission main, the proposed method shows equally distributed pressure sensors in terms of hydraulics. It is expected that the proposed method can be used to manage transmission mains stably and construct a robust real-time network analysis system as a minimal criteria.