• Journal of Science and Technology Studies
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• v.17 no.1
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• pp.71-115
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• 2017

In 1953, theoretical physicist George Gamow attempted to explain the process of protein synthesis by hypothesizing that the base sequence of DNA encodes a protein's amino acid sequence and, in response, proposed the nucleic acid-protein information transfer model, which he dubbed the "diamond code." After expressing interest in discussing the daring hypothesis, contemporary biologists, including James Watson, Francis Crick, Sydney Brenner, and Gunther Stent, were soon invited to join the RNA Tie Club, an informal research group that would also count biologists and various researchers in physics, mathematics, and computer engineering among its members. In examining the club's formation, growth, and decline in multidisciplinary research on deciphering the genetic code in the 1950s, this paper first investigates whether Gamow's idiosyncratic approach could be adopted as a collaborative research forum among contemporary biologists. Second, it explores how the RNA Tie Club's research agenda could have been expanded to other relevant research topics needing multidisciplinary approach? Third, it asks why and how the RNA Tie Club dissolved in the late 1950s. In answering those questions, this paper shows that analyses on the intersymbol correlation of the overlapping code functioned to integrate diverse approaches, including sequence decoding and statistical analysis, in research on the genetic code. As those analyses reveal, the peculiar approaches of the RNA Tie Club could be regarded as a useful method for biological research. The paper also concludes that the RNA Tie Club dissolved in the late 1950s due to the disappearance of the collaborative research agenda when the overlapping code hypothesis was abandoned.

• Journal of Digital Convergence
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• v.16 no.8
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• pp.109-116
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• 2018

This study examines legal regulations and major issues related to income taxation of religion in the income tax law enacted from 2018, and examines the right improvement method. The purpose of this study is to propose an improvement plan through the analytical review of the legal issues related to the income of religion in 2018 and the main issues of taxation. The results of this study are as follows: First, it is the best way to define religious income as a separate item in earned income. Second, it is the best way to apply the deduction system for earned income in the application of deductible expenses. Third, precise and transparent reporting on income of religious persons is required, and a measure should be enforced to impose withholding tax obligations. Fourth, in order to restore transparency and reliability of expenditure related to religious activities, it should be expanded to submit details of total income and expenditure of religious groups. This study provides practical implications for the related field research by providing the starting point and basic data of the discussion on the income taxation system of the religious person.

• Journal of Ginseng Culture
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• v.2
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• pp.17-26
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• 2020

Ginseng, also known as Insam, has solidified its status as one of the supreme drugs classified as life time expansion drug since ancient times and was used as a panacea based on its pharmaceutical effectiveness. The demand for ginseng rapidly increased as the demand for ginseng, which targeted only noble class people, expanded to other social class people. Accordingly, the wild ginseng supply reached its limit due to the extinction caused by indiscriminate harvesting and the difficulty of harvesting, and thus the ginseng supply method shifted to the cultivation of high-priced ginseng rather than complicated wild ginseng foraging. Although the timing of ginseng cultivation (also called gasam) in Korea has been studied, the research on the first cultivation area has not been reported yet. In this study, we critically reviewed Korea's first ginseng cultivation site by arranging data related to ginseng cultivation, and the historical data cited here include Joseon Wangjo Sillog (The Annals of Joseon Dynasty), Imwon Gyeongjeji (The Mid-19 Century Encyclopedia Koreana on Rural Living, Edible plants and Herb Volumes), Oju Yeonmun Jangjeon Sango (An Encyclopedic Writing during the Latter Half Period of the Joseon Dynasty), Junggyeongji (The Junggyeong Town Chronicle), Jeungbo Munheon Bigo (The largest encyclopedia of Joseon Dynasty), Insamsa (The Ginseng History) and etc. As a result of data assortment and critical review, the first ginseng plantation in the Joseon Dynasty resulted in the Yeongnam region, and in Yeongnam region, I have no choice but to conclude that it is Punggi-myeon (currently Punggi-eup, Yeongju-si, Gyeongbuk).

• Journal of the Society of Disaster Information
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• v.16 no.2
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• pp.276-290
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• 2020

Purpose: The construction history of domestic port facilities has been more than 100 years, and until recently, modern facilities have been continuously built and expanded. However, it is not easy to keep the required performance conditions at the time of initial construction due to changes in the marine environment and increase in volume. In particular, in the case of harbor structures that have a long service life, safety performance and function management are becoming very important due to the increase in the size of ships, the increasing frequency of use, and the increase in the scale of natural disasters. Method: Therefore, this study investigates the state change by structural type of port facilities and analyzes the rehabilitation activities and the history that contribute to the performance improvement and life extension activities. Result: Through this, we distinguished between performance improvement cost (CAPEX) and repair maintenance activity (OPEX) that can be used to establish port facility maintenance strategy, and suggested cost model that can be used to establish maintenance strategy. Conclusion: These studies are expected to contribute greatly to mid- to long-term investment decisions.

• Journal of Korean Society of Transportation
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• v.20 no.1
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• pp.77-90
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• 2002

Network expansion has been an inevitable method for most traffic equilibrium assignments to consider intersection movements such as intersection delays. The drawback of network expansion is that because it dramatically increases network sizes to emulate possible directional movements as corresponding links, not only is complexities for building network amplified, but computational performance is shrunk. This paper Proposes a new variational inequality formulation for a user-optimal traffic equilibrium assignment model to explicitly consider directional delays without building expanded network structures. In the formulation, directional delay functions are directly embedded into the objective function, thus any modification of networks is not required. By applying a vine-based shortest Path algorithm into the diagonalization algorithm to solve the problem, it is additionally demonstrated that various loop-related movements such as U-Turn, P-Turn, etc., which are frequently witnessed near urban intersections, can also be imitated by blocking some turning movements of intersections. The proposed formulation expects to augment computational performance through reduction of network-building complexities.

• Archives of design research
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• v.20 no.3 s.71
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• pp.119-128
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• 2007

With the development of digital technologies, CAID became an essential part in the industrial design process. Creating photo-realistic images from a virtual scene with 3D models is one of the specialized task for CAID users. This task requires a complex interface of setting the positions and the parameters of camera and lights for optimal rendering results. However, the user interface of existing CAID tools are not simple for designers because the task is mostly accomplished in a parameter setting dialogue window. This research address this interface issues, in particular the issues related to lighting, by developing and evaluating TLS(Tangible Lighting Studio) that uses Augmented Reality and Tangible User Interface. The interface of positioning objects and setting parameters become tangible and distributed in the workspace to support more intuitive rendering task. TLS consists of markers, and physical controller, and a see-through HMD(Head Mounted Display). The user can directly control the lighting parameters in the AR workspace. In the evaluation experiment, TLS provide higher effectiveness, efficiency and user satisfaction compared to existing GUI(Graphic User Interface) method. It is expected that the application of TLS can be expanded to photography education and architecture simulation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.2
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• pp.221-227
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• 1995

White clover (Trifolium repens L) gives rise to either weak persistence or overdominance in the pastures. To get information on grazing method to surmount the problem, the experiment was done to measure the effect of infrequent, frequent and their alternations on harvest yield and morphological characteristics of the clover. Individual plants of Regal, Louisiana S-l, Grasslands Huia, and Aberystwyth S184 were grown in 22cm plastic pot containing a 2： 1： 1 soil： sand： Peat moss mixture for 27 days after transplanting 50-day seedlings raised on 3cm pots, and then their all fully expanded leaves are trimmed. Defoliation treatments were forced every 1 (CC, frequent), 4 week(RR,infrequent) or their alternations(CR, RC) after 8 weeks from the trimming. To analyze the treatment effects, plants were sampled on 4, 8, 12, and 16 weeks after the trimming. Harvest yield of infrequent defoliation (RR) was higher than that of frequent defoliation (CC). Leaf area and no. of leaves per plant, petiole length and stolon length per plant, moreover, showed the similar result to the yield but stolon length and leaf area per g were reverse. The alternation of infre-quent and then frequent defoliation (RC) had greater yield than that of their reverse (CR) although both alternations showed intermediate ones compared to CC and RR. Morphological characteristics, furthermore, related to the clover leaf were immediately changed by alternations of defoliation inter-val(RC and CR) while those done to the stolon were lasted longer thereafter(RC and CR). Harvest yields in RC and CR were positively correlated to leaf area and no. of leaves or stolon length per plant but negatively done to leaf area and stolon length per g. It is concluded that weak persistence or overdominance of white clover in pasture can be controlled by alternation of infrequent and then frequent defoliation or reverse.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.1
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• pp.19-27
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• 2004

Objective: This study was to examine the in vitro neural cell differentiation patterns of human embryonic stem (hES) cells following treatment of various neurotrophic factors [basic fibroblast growth factor (bFGF), retinoic acid (RA), brain derived neurotrophic factor (BDNF) and transforming growth factor (TGF)-$\alpha$], particulary in dopaminergic neuron formation. Methods: The hES cells were induced to differentiate by bFGF and RA. Group I) In bFGF induction method, embryoid bodies (EBs, for 4 days) derived from hES were plated onto gelatin dish, selected for 8 days in ITSFn medium and expanded at the presence of bFGF (10 ng/ml) for another 6 days followed by a final differentiation in N2 medium for 7, 14 and 21 days. Group II) For RA induction, EBs were exposed of RA ($10^{-6}M$) for 4 days and allowed to differentiate in N2 medium for 7, 14 and 21 days. Group III) To examine the effects of additional neurotrophic factors, bFGF or RA induced cells were exposed to either BDNF (10 ng/ml) or TGF-$\alpha$ (10 ng/ml) during the 21 days of final differentiation. Neuron differentiation and dopamine secretion were examined by indirect immunocytochemistry and HPLC, respectively. Results: The bFGF or RA treated hES cells were resulted in similar neural cell differentiation patterns at the terminal differentiation stage, specifically, 75% neurons and 11% glial cells. Additionally, treatment of hES cells with BDNF or TGF-$\alpha$ during the terminal differentiation stage led to significantly increased tyrosine hydroxylase (TH) expression of a dopaminergic neuron marker, compared to control (p<0.05). In contrast, no effect was observed on the rate of mature neuron (NF-200) or glutamic acid decarboxylase-positive neurons. Immunocytochemistry and HPLC analyses revealed the higher levels of TH expression (20.3%) and dopamine secretion (265.5 $\pm$ 62.8 pmol/mg) in bFGF and TGF-sequentially treated hES cells than those in $\alpha$ RA or BDNF treated hES cells. Conclusion: These results indicate that the generation of dopamine secretory neurons from in vitro differentiated hES cells can be improved by TGF-$\alpha$ addition in the bFGF induction protocol.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.1
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• pp.67-74
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• 2004

Objective: This study was to examine in vitro neural cell differentiation pattern of the genetically modified human embryonic stem cells expressing tyrosine hydroxylase (TH). Materials and Methods: Human embryonic stem (hES, MB03) cell was transfected with cDNAs cording for TH. Successful transfection was confirmed by western immunoblotting. Newly transfected cell line (TH#2/MB03) was induced to differentiate by two neurogenic factors retinoic acid (RA) and b-FGF. Exp. I) Upon differentiation using RA, embryoid bodies (EB, for 4 days) derived from TH#2/MB03 cells were exposed to RA ($10^{-6}M$)/AA ($5{\times}10^{-2}mM$) for 4 days, and were allowed to differentiate in N2 medium for 7, 14 or 21 days. Exp. II) When b-FGF was used, neuronal precursor cells were expanded at the presence of b-FGF (10 ng/ml) for 6 days followed by a final differentiation in N2 medium for 7, 14 or 21 days. Neuron differentiation was examined by indirect immunocytochemistry using neuron markers (NF160 & NF200). Results: After 7 days in N2 medium, approximately 80% and 20% of the RA or b-FGF induced Th#2/MB03 cells were immunoreactive to anti-NF160 and anti-NF200 antibodies, respectively. As differentiation continued, NF200 in RA treated cells significantly increased to 73.0% on 14 days compared to that in b-FGF treated cells (53.0%, p<0.05), while the proportion of cells expressing NF160 was similarly decreased between two groups. However, throughout the differentiation, expression of TH was maintained ($\sim$90%). HPLC analyses indicated the increased levels of L-DOPA in RA treated genetically modified hES cells with longer differentiation time. Conclusion: These results suggested that a genetically modified hES cells (TH#2/MB03) could be efficiently differentiated in vitro into mature neurons by RA induction method.

• Journal of the Korean Association of Geographic Information Studies
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• v.18 no.2
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• pp.149-160
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• 2015

This study aims to estimate the forest volumes of the upper region of Nam-Han River in ecosystem zoning by forest types and age classes, and to suggest the optimal estimation method through the comparison of the standard errors according to the spatial unit. In the estimation of forest volumes, we used both of direct estimation, which uses sample plots of the target area only, and synthetic estimation, which includes sample plots of the expanded areas as well as those of the target area. As for the spatial expansion, we applied four standards for synthetic estimator: Mountainous zone, Neighbor ecosystem region, Gangwon province, and Buffer zone. The results show that average forest volume per ha, calculated by direct estimation, was $143.5m^3/ha$, while that by synthetic estimation with each standard, was estimated at $146.9m^3/ha$ by Gangwon province, $144.8m^3/ha$ by Buffer zone, $139.8m^3/ha$ by Neighbor ecosystem region, and $138.6m^3/ha$ by Mountainous zone, respectively. The standard errors of direct estimation was $1.79m^3/ha$, while those of synthetic estimation showed not a great difference among the errors. Meanwhile, considering the standard errors by forest type, the lowest was ${\pm}2.3m^3/ha$ of broad-leaved forest, followed by ${\pm}3.3m^3/ha$ of mixed forest, and ${\pm}4.8m^3/ha$ of coniferous forest.