• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.989-998
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• 2019

Autophagy is crucial for immune defense against Mycobacterium tuberculosis (Mtb) infection. Mtb can evade host immune attack and survival within macrophages by manipulating the autophagic process. MicroRNAs (miRNAs) are small, non-coding RNAs that are involved in regulating vital genes during Mtb infection. The precise role of miRNAs in autophagy with the exits of Mtb remains largely unknown. In this study, we found miR-1958, a new miRNA that could regulate autophagy by interacting with 3'UTR of autophagy-related gene 5 (Atg5). In addition, Mtb infection triggered miR-1958 expression in RAW264.7 cells. What's more, miR-1958 overexpression blocked autophagic flux by impairing the fusion of autophagosomes and lysosomes. Overexpression of miR-1958 reduced Atg5 expression and LC3 puncta while inhibition of miR-1958 brought an increase of Atg5 and LC3 puncta; the opposite results were observed in detection of p62. The survival of Mtb in RAW264.7 cells transfected with mimic of miR-1958 was enhanced. Taken together, our research demonstrated that a novel miR-1958 could inhibit autophagy by interacting with Atg5 and favored intracellular Mtb survival in RAW264.7 cells.

• Parasites, Hosts and Diseases
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• 제57권2호
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• pp.127-134
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• 2019

Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient morphological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary bladder of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were observed under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring $13.9{\pm}0.6{\mu}m$ in length and $13.8{\pm}0.8{\mu}m$ in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of $4.3{\pm}0.4{\mu}m$. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsularis. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.

• International Journal of High-Rise Buildings
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• 제10권1호
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• pp.1-8
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• 2021

Currently, big data and open data, together with traditional measured data, have come to constitute a new data environment, expanding new technical paths for quantitative analysis of the street environment. Streets provide precious linear public space in high-density residential areas. Pedestrian activities are the main body of street vitality. In this paper, 441 street segments were selected from 21 residential districts in high-density downtown area of Shanghai as cases, to quantitatively evaluate the influencing factors of pedestrian activities. Bivariate analysis was performed, and the results showed that street vitality was not only correlated with a highly populated environment, but also with other factors. In particular, the density of entrances and exits of residential properties, the proportion of walkable areas, and the density of retail and service facilities, were correlated with the vitality of street segments. The magnitudes of correlation between the street environmental factors and the pedestrian traffic differed across various trip purposes. Segment connectivity factors were more correlated with walking for leisure than for transportation. While public transportation factors were mainly correlated with walking for transportation, vehicular traffic factors were negatively correlated with walking for leisure.

• Journal of Korean Medical classics
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• 제35권1호
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• pp.137-153
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• 2022

Objectives : This paper aims to examine the meaning of guanjie(關節) in the Huangdineijing. Methods : Meanings of guan(關) and jie(節) were examined within the Neijing, after which the meaning of guanjie was studied in reference to usages of guan and jie. Other terms used similarly to guanjie were also studied. Results & Conclusions : 1. The meaning of guanjie in the Huandineijing could be inferred to meanings such as 'vital point and node', 'node of entrance gate', 'entrance gate and node', 'bolt and node', 'node of bolt', etc. 2. Most usages of guanjie in the Huangdineijing referred to 'pivotal node', while those mentioned in relation to edema or joint pain from wind and dampness meant 'node of entrance gate.' 3. The four entrances, eight nodes, extremity node, eight hollows, eight nodes of the limbs, twelve nodes, which are used similarly to guanjie, manage the five zang, communicate intimately via qi, while shen qi or zhen qi enter and exit. 4. Guanjie is closely linked to the five zang, while it is the entrance gate or pivotal node through which shen qi roams and travels. Therefore when interpreting a verse that includes 'guanjie', one must be aware of the perspectives of the Huangdineijing on qi, keeping in mind the close relationship with the five zang, and that zhen qi enters and exits.

• Journal of Korea Trade
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• 제26권1호
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• pp.1-19
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• 2022

Purpose - This paper analyzes the economic and non-economic factors that contribute to environmental sustainability by reducing CO₂ emissions, based on G20 panel data. Design/methodology - We conduct a comparative analysis of advanced and developing economies during 1995-2016. To examine the impact, an environmental Kuznets curve (EKC) model was employed, incorporating additional explanatory variables such as internet use, renewable energy, and services trade. Findings - The empirical findings show the existence of an inverted U-shaped EKC phenomenon between GDP per capita and CO₂ emissions in G20 economies, with the turning point at a per capita GDP level of US$ 38,340. Moreover, an inverted U-shape relation exists between internet use and CO₂ emissions, with the turning point at a 44% internet use rate. The comparative analysis show that the inverted U-shape curve only exits in advanced economies, with turning points of US$ 42,356 per capita GDP and 27% internet use rate, respectively. Renewable energy and services trade have a greater negative impact on CO₂ emissions in advanced economies than in developing economies. Originality/value - Renewable energy and services trade have a greater negative impact on CO₂ emissions in advanced economies than in developing economies. Overall, the results suggest the role of internet use, renewable energy and services trade in sustainable development in G20 countries.

• Kyungpook Mathematical Journal
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• 제62권1호
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• pp.119-132
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• 2022

Virotherapy is an effective method for the treatment of cancer. The oncolytic virus specifically infects the lyse cancer cell without harming normal cells. There is a time delay between the time of interaction of the virus with the tumor cells and the time when the tumor cells become infectious and produce new virus particles. Several types of viruses are used in virotherapy and the delay varies with the type of virus. This delay can play an important role in the success of virotherapy. Our present study is to explore the impact of this delay in cancer virotherapy through a mathematical model based on delay differential equations. The partial success of virotherapy is guarenteed when one gets a stable non-trivial equilibrium with a low level of tumor cells. There exits Hopf-bifurcation by considering the delay as bifurcation parameter. We have estimated the length of delay which preserves the stability of the non-trivial equilibrium point. So when the delay is less than a threshold value, we can predict partial success of virotherapy for suitable sets of parameters. Here numerical simulations are also performed to support the analytical findings.

• Proceedings of the Korean Institute of Building Construction Conference
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• 한국건축시공학회 2021년도 가을 학술논문 발표대회
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• pp.92-93
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• 2021

In the case of medical facilities, the evacuation time is delayed due to the decrease in the number of people in the hallway and exits due to the increase in the width of evacuation by using mobile beds, wheelchairs, crutches, etc. Accordingly, it is considered to secure evacuation capacity to reduce evacuation time according to corridor width and exit width. Accordingly, we would like to compare the standards related to the evacuation capacity of medical facilities in Korea and NFPA, derive differences, and use evacuation simulations to compare evacuation times according to changes in corridor width and exit width. In Korea, it is calculated based on the floor area by use, but in the case of NFPA 101, the number of evacuation routes, stair width, corridor exit width, and two-way door width was stipulated depending on the number of people. Using evacuation simulation, efficient evacuation capacity is calculated according to the reduction of evacuation time by changing the width of the hallway, changing the width of the exit, the width of the corridor, and the width of the exit. The evacuation simulation is intended to be used to secure evacuation safety of domestic medical facilities by calculating the effective evacuation time reduction by changing the width of the hallway and exit.

• The Journal of the Convergence on Culture Technology
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• 제9권4호
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• pp.617-623
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• 2023

Among the fire incidents in Korea over the past decade, building fires are the most common, and property and human casualties are the most common. However, the existing fire fighting system does not only inform the location of emergency exits and guide safe routes to help casualties evacuate smoothly. A system was proposed to help successful evacuation by distinguishing vertical and horizontal characteristics using spatial characteristics. In this study, an effective evacuation system was proposed by predicting fires using temperature detection sensors and smoke sensor values, and calculating the optimal evacuation path through the Dijkstra algorithm.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.79-79
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• 2020

Halophyte is a plant that has evolved to grow well in salty places, and is mainly distributed in coastal wetlands, sand dunes, salt fields, and reclaimed lands in tidal flats and river estuaries. Because it grows in the soil where seawater enters and exits, it is very abound in natural minerals and produces certain metabolites to survive sustainably in the surrounding environment. In this study, anti-inflammatory studies were conducted using 15 kinds of halophyte to secure data on halophytes of infinite value as functional materials. The aim of this study was to select a group of halophytes that regulate iNOS expression, which is involved in the secretion of inflammatory cytokines and nitric oxide production in LPS-induced macrophages. Among the 15 species of halophyte, except for Triglochin maritimum, Suaeda japonica, and Carex pumila, NO production was reduced in 12 species of halophytes, and 7 species of halophyte (Suaeda asparagoides, Artemisia fukudo, Spergularia marina, Aster tripolium, Suaeda australis, Atriplex subcordata, Calystegia soldanella) significantly decreased the expression levels of TNF-α and IL-1β.

• Journal of Koreanology Reviews
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• 제2권2호
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• pp.27-34
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• 2023

Purpose: The purpose of this study is to discuss on Daeryuk Baekje (the Continental Baekje), as new researches are beginning to emerge on the existing theory that Baekje exits only in the Korean Peninsula. Research design, data and methodology: We intend to systematically reveal this new theory by carefully examining the early founding process of Baekje, the process of determining its capital city and the ancient documents related to it. Objective analysis and research were conducted through literature research including not only domestic research data but, also research data from overseas. Results: Baekje existed on the Continent and on the Peninsula in the early days of Baekje establishment, before the Kingdom was established, so-called Hanseong Baekje. Conclusion: Biryu and Onjo settled down near Yoseo and Jinpyeong when they first moved to the south from Goguryeo. Biryu led hundred families to Michuhol and established Baekje. Onjo established Sipje at the Wirye Castle with ten of his servants. After Biryu's death the people of Baekje pledge allegiance to Onjo. The Sipje changed its country's name to Baekje. This illustrates that the country was operated with a capital in two regions, in the Continent and the Korean Peninsula. The country was ruled under two royal castles with 22 provincial governments (Dam-ro). Each area was ruled by the royal families and the competent prince among them succeeded to the next throne. It is a unique governing system and illustrates that Baekje existed in the Continent.