• Title/Summary/Keyword: excess of existence

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Production of Mn-Dependent Peroxidase from Bjerkandera fumosa and Its Enzyme Characterization

  • Jarosz-Wilkolazka, Anna;Luterek, Jolanta;Malarczyk, Elzbieta;Leonowicz, Andrzej;Cho, Hee-Yeon;Shin, Soo-Jeong;Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.2
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    • pp.85-95
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    • 2007
  • Manganese dependent peroxidase (MnP) is the most ubiquitous enzyme produced by white-rot fungi, MnP is known to be involved in lignin degradation, biobleaching and oxidation of hazardous organopollutants. Bjerkandera fumosa is a nitrogen-unregulated white-rot fungus, which produces high amounts of MnP in the excess of N-nutrients due to increased biomass yield. The objective of this study was to optimize the MnP production in N-sufficient cultures by varying different physiological factors such as Mn concentration, culture pH, and incubation temperature. The growth of fungus was optimal in pH 4.5 at $30^{\circ}C$, $N_2$-unregulated white-rot fungus produces high amounts of MnP in the excess N-nutrients. The fungus produced the highest level of MnP (up to $1000U/{\ell}$) with $0.25g/{\ell}$ asparagine and $1g/{\ell}$ $NH_4Cl$ as N source at 1.5 mM $MnCl_2$ concentration, pH value of 4.5 at $30^{\circ}C$. Purification of MnP revealed the existence of two isoforms: MnPl and MnP2. The molecular masses of the purified MnPl and MnP2 were in the same range of 42~45 kDa. These isoforms of B. fumosa strictly require Mn to oxidize phenolic substrates. Concerned to kinetic constants of B. fumosa MnPs, B. fumosa has similar Km value and Vmax compared to the other white-rot fungi.

Mesothelioma in Sweden: Dose-Response Analysis for Exposure to 29 Potential Occupational Carcinogenic Agents

  • Plato, Nils;Martinsen, Jan I.;Kjaerheim, Kristina;Kyyronen, Pentti;Sparen, Par;Weiderpass, Elisabete
    • Safety and Health at Work
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    • v.9 no.3
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    • pp.290-295
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    • 2018
  • Background: There is little information on the dose-response relationship between exposure to occupational carcinogenic agents and mesothelioma. This study aimed to investigate this association as well as the existence of agents other than asbestos that might cause mesothelioma. Methods: The Swedish component of the Nordic Occupational Cancer (NOCCA) study consists of 6.78 million individuals with detailed information on occupation. Mesothelioma diagnoses recorded in 1961-2009 were identified through linkage to the Swedish Cancer Registry. We determined cumulative exposure, time of first exposure, and maximum exposure intensity by linking data on occupation to the Swedish NOCCA job-exposure matrix, which includes 29 carcinogenic agents and corresponding exposure for 283 occupations. To assess the risk of mesothelioma, we used conditional logistic regression models to estimate hazard ratios and 95% confidence intervals. Results: 2,757 mesothelioma cases were identified in males, including 1,416 who were exposed to asbestos. Univariate analyses showed not only a significant excess risk for maximum exposure intensity, with a hazard ratio of 4.81 at exposure levels 1.25-2.0 fb/ml but also a clear dose-response effect for cumulative exposure with a 30-, 40-, and 50-year latency time. No convincing excess risk was revealed for any of the other carcinogenic agents included in the Swedish NOCCA job-exposure matrix. Conclusion: When considering asbestos exposure, past exposure, even for short periods, might be enough to cause mesothelioma of the pleura later in life.

Effect of Seizure on Prognosis in Acute Endosulfan Intoxication (급성 endosulfan 중독환자에서 경련이 예후에 미치는 영향)

  • Han, Byung-Gon;Lee, Jun-Ho;Lee, Kyung-Woo
    • Journal of The Korean Society of Clinical Toxicology
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    • v.7 no.2
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    • pp.77-82
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    • 2009
  • Purpose: In highly doses, endosulfan lowers the seizure threshold and elicits central nervous system stimulation, which can result in seizures, respiratory failure, and death. Management of seizure control is essential for survival and prognosis of intoxicated patients. This study assessed whether seizure time was an independent predictor mortality in patients with endosulfan poisoning. Methods: This retrospective study enrolled patients with endosulfan poisoning presenting to Masan Samsung Hospital and Gyeongsang National University Hospital from January 2003 to December 2008. The data were collected from clinical records and laboratory files. Using a multivariate logistic analysis, data on the total population was retrospectively analyzed for association with mortality. Results: Of the 24 patients with endosulfan poisoning, nineteen (79.1%) experienced seizure. The patients in the seizure group showed significantly lower Glasgow coma scale score, base excess, bicarbonate, and significant existence of mechanical ventilation, as compared to the non seizure group (n=5). Seizure, Glasgow coma scale score, systolic blood pressure, bicarbonate level, need for respiratory support, pulse rate, respiratory rate, pH, base excess, and seizure time were associated with mortality. The fatality rate of endosulfan poisoning was 54.1% with higher mortality among patients experiencing. Longer seizure time was associated with higher mortality. Conclusion: Seizure time can be a significant independent predictor of mortality in patients with acute endosulfan poisoning. Physicians should aggressively treat for seizure control in patients with acute endosulfan poisoning.

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Effects of Brassiea campestris L. and Lactuca sativa L. Yield by Application of Organic Fertilizers and Microorganisms (유기질비료와 토양 미생물제제의 시용이 배추(Brassiea campestris L.)와 상추(Lactuca sativa L.)의 수량에 미치는 영향)

  • Jo, Sung-Hyun;Park, Tae-Hurn;Chang, Ki-Woon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.3
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    • pp.88-92
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    • 2001
  • This study was carried out to investigate about the effect of organic fertilizers(Pig manure fermentation compost; P, Organic fertilizer; B, F, Compost; D, and microorganism; M) on the growth and yield of the chinese cabbage and lactuca. At the standard applicated-amounts plot of organic fertilizer have appeared the best yield, but another the excess amount plot came into existence damage of fertilizer. However, according to the co-application of microorganism appeared certainly decreasing damage of fertilizers. Also at the same fertilizer and applicated-amounts plots were the higher than an-application of microorganism on the growth and yield. In conclusion, the excess application of organic fertilizer should be occurred fertilizer damages and increased on the growth and yield by use of microorganism.

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An Analysis of the Settlement Behavior of Soft Clayey Ground Considering the Effect of Creep during the Primary Consolidation (1차압밀과정중의 크리프의 영향을 고려한 연약 점성토지반의 침하거동 해석)

  • Baek, Won-Jin;Matsuda, Hiroshi;Choi, Woo-Jung;Kim, Chan-Kee;Song, Byung-Gwan
    • Journal of the Korean Geotechnical Society
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    • v.24 no.5
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    • pp.107-115
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    • 2008
  • This paper is performed to examine the effect of creep during the primary consolidation and the applicability of the Yin's EVP (Elasto-Visco-Plastic) model. In ordinary consolidation theories using the elastic model, the primary consolidation process can be expressed but the secondary consolidation process cannot. It is due to the viscosity, which can express the secondary consolidation, and is sometimes related to the scale effect (difference of the thickness of clay layer between laboratory sample and field condition) such as hypotheses Type A and Type B shown by Ladd et al. (1977). Usually, the existence of the creep during the primary consolidation has been conformed and the Type B is well acceped. On the other hand, from the large-scaled consolidation tests the intermediate characteristic between Type A and Type B was proposed as Type C by Aboshi (1973). In this study, to clarify the effect of creep on the settlement-time relation during the primary consolidation in detail, Type B consolidation tests were performed using the separate-type consolidation test apparatus for a peat and clay. Then the test results were analyzed by using Yin's EVP Model (Yin and Graham, 1994). In conclusion, followings were obtained. At the end of primary consolidation, the compression for the subspecimens should not be the same because of the difference of the excess pore water pressure dissipation rate. And the average settlement measured by the separate-type consolidometer coincides with the analyzed one using the Yin's EVP model. As for the dissipation of the excess pore water pressure, however, the measured excess pore water pressure dissipates faster compared with the Yin's model.

Spectroscopy of Local Starburst Galaxies (가까운 폭발적 항성생성은하의 분광 관측)

  • Lee, Cheolhui;Shim, Hyunjin
    • Journal of the Korean earth science society
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    • v.38 no.3
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    • pp.209-221
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    • 2017
  • We investigate the star formation rate, stellar mass, and gas-phase metallicity of local starburst galaxies with different star formation time scales based on their optical spectra. The observation is made using the longslit spectrograph attached to the 4K CCD on the Bohyunsan Optical Astronomy Observatory 1.8m telescope, targeting 21 Wolf-Rayet galaxies as young starbursts and 13 UV excess galaxies as slightly older starbursts. A Baldwin-Phillips-Terlevich diagram analysis shows that 50% of the observed targets are pure star-forming galaxies while only 15% are classified as Active Galactic Nuclei. Fraction of galaxies that reside in composite region is higher in UV excess galaxies than in Wolf-Rayet galaxies, suggesting that the AGN development requires extra time after the onset of the star formation. Most of the observed starburst galaxies have stellar masses of $10^{9-11}M_{\odot}$ and stellar formation rates of $0.01-100M_{\odot}yr^{-1}$, and their star formation rates are consistent with that of the SDSS star forming main sequence galaxies of similar stellar mass. There is no significant difference between Wolf-Rayet galaxies and UV excess galaxies in terms of the stellar mass and star formation rate. We also see a mass-metallicity relation for local starbursts with slightly lower metallicity for a given stellar mass, which implies the existence of a strong feedback activity due to the star formation in these galaxies.

Improvement of Magnetic Properties and Texture of FePt Thin Films on MgO Substrates by Sn Addition

  • Chun, Dong-Won;Kim, Sung-Man;Kim, Gyeung-Ho;Jeung, Won-Young
    • Journal of Magnetics
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    • v.14 no.1
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    • pp.7-10
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    • 2009
  • In this work, we studied the effects of Sn addition on the ordering temperature of FePt thin film. The coercivity of FePtSn film was about 1000 Oe greater than the coercivity of FePt film for an annealing temperature of $600^{\circ}C$. Therefore, Sn addition was effective in promoting the $L1_0$ ordering and in reducing the ordering temperature of the FePt film. From our X-ray diffraction results, we found that in the as-deposited film, the addition of Sn induced a lattice expansion in disordered FePt thin films. After the annealing process, the excess Sn diffuses out from the ordered FePt thin film because of the difference in the solid solubility of Sn between the disordered and ordered phases. The existence of precipitates of Sn from the FePt lattice was deduced by Curie temperature measurements of the FePt and FePtSn films. Therefore, the key role played by the addition of Sn to the FePt film can be explained by a reduction in the activation energy for the $L1_0$ order-disorder transformation of FePt which originates from the high internal stress in the disordered phase induced by the supersaturated Sn atoms.

A Study on Discrimination of Watercore Apple using Transmitted Light and Effects of various Factors (투과광을 이용한 밀병 사과의 판별 가능성 및 영향인자 조사)

  • 손미령;정경원;조래광
    • Food Science and Preservation
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    • v.7 no.4
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    • pp.357-361
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    • 2000
  • Watercore influences storage and distribution industry of fruit. Therefore, the technique for nondestructive discrimination of watercore fruit is needed. This work reports about the possibility of watercore discrimination of ante using transmitted light, and the effects of various factors. CCD camera was used to capone images of each apple fruit. An excess watercore apples were higher light transmission score than little watercore apples. The accuracy fur discrimination of watercore apple was about 70% using transmitted light. Peel thickness, anthocyanin layer thickness and density of apple affected the light transmission. Apples having thin peel, thin pigment layer and low density tended to high transmitted light score. Apples of good color degree were more probability of existence watercore than ones of bad color degree. But color distribution of apple peel was not correlated with watercore.

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Microstructure of Glass-ceramics Made from Bottom Ash Produced at a Thermal Power Plant (화력발전소 바닥재로 제조된 결정화 유리의 미세구조)

  • Kang, Seung-Gu
    • Korean Journal of Materials Research
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    • v.19 no.2
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    • pp.95-101
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    • 2009
  • Glass ceramics were made from coal bottom ash by adding CaO and $Li_2O$ as glass modifiers and $TiO_2$ as a nucleating agent in a process of melting and quenching followed by a thermal treatment. The surface of the glass ceramics has 1.6 times more $Li_2O$ compared to the inner matrix. When $TiO_2$ was not added or when only 2 wt% was added, the surface parts of the glass ceramics were crystalline with a thickness close to $130{\mu}m$. In addition, the matrixes showed only the glass phase and not the crystalline phase. However, doping of $TiO_2$ from 4 wt% to 10 wt% began to create small crystalline phases in the matrix with an increase in the quantity of the crystalline. The matrix microstructure of glass ceramics containing $TiO_2$ in excess of 8 wt% was a mixture of dark-gray crystalline and white crystalline parts. These two parts had no considerable difference in terms of composition. It was thought that the crystallization mechanism affects the crystal growth, direction and shape and rather than the existence of two types of crystals.

The Swelling Characteristics of Clayey Soil by CRS Consolidation Test (CRS 압밀시험에 의한 점성토의 팽창특성에 관한 연구)

  • 이응준;한상재;김지용;김수삼
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.11a
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    • pp.335-342
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    • 2000
  • In this study, the swelling characteristics of reconstituted clayey soil were investigated by STD and CRS test. The strain rate during loading was constant i.e. 0.05 %/min, 0.03 %/min and during unloading was varied in proportion to 1/1, 1/5, 1/10 and 1/15 of strain rate during loading. From this study the following conclusions were obtained; (1) There were similar values, especially, during unloading in case of 1/10 or 1/15 of strain rate during loading and the test results between STD and CRS were much to be alike. (2) The cross point of effective stress versus excess pore water pressure ratio curve, was increased during unloading, while the stress level of the cross point was decreased. The stress level can be separated into two zones according to the swelling index named Cs1 and Cs2. From the test results, the values of Cs1 were approximately constant irrespective of strain rate during unloading, but the values of Cs2 were much influenced by strain rate. (3) In CRS consolidation tests, it was found that unloading strain rate did not affect on the existence of zone.

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