• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1566-1575
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• 2017

MicroRNAs (miRNAs) are abundant in bovine milk and milk derived from other livestock, and they have functional roles in infants and in the secretion process of mammary glands. However, few studies have evaluated miRNAs in dairy processes, such as during cheese making and ripening. Thus, we investigated the characteristics of milk-derived miRNAs during the manufacturing and ripening of Camembert cheese as well as the microbiota present using the quantitative reverse transcription polymer chain reaction (RT-qPCR) and 16S rRNA pyrosequencing, respectively. Pyrosequencing showed that the cheese microbiota changed dramatically during cheese processing, including during the pasteurization, starter culture, and ripening stages. Our results indicated that the RNA contents per $200mg/200{\mu}l$ of the sample increased significantly during cheese-making and ripening. The inner cheese fractions had higher RNA contents than the surfaces after 12 and 22 days of ripening in a time-dependent manner (21.9 and 13.2 times higher in the inner and surface fractions than raw milk, respectively). We performed a comparative analysis of the miRNAs in each fraction by RT-qPCR. Large amounts of miRNAs (miR-93, miR-106a, miR-130, miR-155, miR-181a, and miR-223) correlated with immune responses and mammary glands were present in aged cheese, with the exception of miR-223, which was not present on the surface. Considerable amounts of miRNAs were also detected in whey, which is usually disposed of during the cheese-making process. Unexpectedly, there were no significant correlations between immune-related miRNAs and the microbial populations during cheese processing. Taken together, these results show that various functional miRNAs are present in cheese during its manufacture and that they are dramatically increased in amount in ripened Camembert cheese, with differences according to depth.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.2
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• pp.1477-1482
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• 2015

In order to find out the effect of embedment ratio on behavior compensated foundation, numerical analyses were performed. Bearing capacity ratios obtained from numerical analyses were greater than those obtained from theoretical equations and it could be seen that the bearing capacity ratio was proportional to the embedment ratio with only exception of the case of square footing in which bearing capacity ratio was increased rapidly with the embedment ratio. For the case of strip footing on sand, the bearing capacity ratios obtained from the numerical analyses and Meyerhof equation were similar with each other and magnitudes of those were as much as square of the embedment ratio but the bearing capacity ratios were little affected by the embedment ratios for the case of strip footing on clay. It can be said that the bearing capacity ratios obtained from the square footing are greater than those obtained from the strip footing. According to the numerical analysis, values of settlement ratios which correspond to the embedment ratio of one were about 0.4 and settlement ratios were decreased with increase of the embedment ratios. Settlement ratios of the loose sand were smaller than those of the dense sand and the clay.

• Journal of Korea Water Resources Association
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• v.52 no.10
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• pp.729-742
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• 2019

Natural disasters such as floods has been increased in many parts of the world, also Korea is no exception. The biggest part of natural damage in South Korea was caused by the flooding during the rainy season in every summer. The existing flood vulnerability analysis cannot explain the reality because of the repeated changes in topography. Therefore, it is necessary to calculate a new flood vulnerability index in accordance with the changed terrain and socio-economic environment. The priority of the investment for the flood prevention and mitigation has to be determined using the new flood vulnerability index. Total 25 urban districts in Seoul were selected as the study area. Flood vulnerability factors were developed using Pressure-State-Response (PSR) structures. The Pressure Index (PI) includes nine factors such as population density and number of vehicles, and so on. Four factors such as damage of public facilities, etc. for the Status Index (SI) were selected. Finally, seven factors for Response Index (RI) were selected such as the number of evacuation facilities and financial independence, etc. The weights of factors were calculated using AHP method and Fuzzy AHP to implement the uncertainties in the decision making process. As a result, PI and RI were changed, but the ranks in PI and RI were not be changed significantly. However, SI were changed significanlty in terms of the weight method. Flood vulnerability index using Fuzzy AHP shows less vulnerability index in Southern part of Han river. This would be the reason that cost of flood mitigation, number of government workers and Financial self-reliance are high.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.49 no.8
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• pp.671-680
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• 2021

This paper deals with the contents of designing and producing reconnaissance hardware and software, and verifying the functions after being installed on the CanSat platform and ground stations. The main reconnaissance mission is largely composed of two things: terrain search that renders the surrounding terrain in 3D using radar, GPS, and IMU sensors, and real-time detection of major objects through optical camera image analysis. In addition, data analysis efficiency was improved through GUI software to enhance the completeness of the CanSat system. Specifically, software that can check terrain information and object detection information in real time at the ground station was produced, and mission failure was prevented through abnormal packet exception processing and system initialization functions. Communication through LTE and AWS server was used as the main channel, and ZigBee was used as the auxiliary channel. The completed CanSat was tested for air fall using a rocket launch method and a drone mount method. In experimental results, the terrain search and object detection performance was excellent, and all the results were processed in real-time and then successfully displayed on the ground station software.

• Analytical Science and Technology
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• v.25 no.4
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• pp.257-264
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• 2012

Penicillins belong to the ${\beta}$-lactam class of antibiotics, and are frequently used in human and veterinary medicine. Despite the positive effects of these drugs, improper use of penicillins poses a potential health risk to consumers. This study has been undertaken to determinate multi-residues of penicillins, including amoxicillin, ampicillin, oxacillin, bezylpenicillin, cloxacillin, dicloxacillin, and nafcillin, using liquid chromatographic tandem mass spectrometer (LC-MS/MS). The developed method was validated for specificity, precision, recovery, and linearity in livestock and marine products. The analytes were extracted with 80% acetonitrile and clean-up by a single reversed-phase solid-phase extraction step. Six penicillins presented recoveries higher than 76% with the exception of Amoxicillin. Relative standard deviations (RSDs) were not more than 10%. The method was applied to 225 real samples. Benzylpenicillin was detected in 12 livestock products and 7 marine products. Amoxicillin, ampicillin, cloxacilllin, dicloxacillin, nafcillin and oxacillin were not detected. The detected levels were 0.001~0.009 mg/kg in livestock products excluding eggs and milk. In marine products, the detected levels were under 0.03 mg/kg. They were under the MRL levels. As monitoring results, it is identified to be safe but it is considered that safety management of antibiotics should continue by monitoring.

• Journal of Ginseng Research
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• v.22 no.4
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• pp.274-283
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• 1998

It has generally been accepted that quality of ginseng should be determined not by the content of a single component but by composition and balance of total active principles. However, there still can be an exception with a product in which a given ginsenoside is used for the treatment of a specific disease. Although ginsenosides have been regarded to be major active components of ginseng and employed as index components for the quality control, it does not consistent with the traditional concept on ginseng quality creterion; main root has been more highly appreciated than the lateral or fine root. Content of ginsenosides in the lateral or fine root is much higher than that in main root. However, the ratio of protopanaxadiol (PD) and protopanaxatriol (PT) saponins existing in various part of ginseng root is greatly different. The ratio of PD/PT saponins in main root is well balanced but the thinner the root is the higher the ratio. Thus far, a total of 34 different kinds of ginsenosides have been isolated from Korean (red) ginseng, and their pharmacological activities were elucidated partly. Interestingly, different ginsenoside shows similar or contrary effects to each other in biological systems, thus indicating the significance of absolute content of single ginsenoside as well as compositional patterns of each ginsenoside. Therefore, pharmacological activities of ginseng should be determined as a wholly concept. In these regards, standardization of ginseng material (fresh ginseng root) should be preceded to the standardization of ginseng products because ginsenoside content and non-saponin active principles such as polysaccharides and nitrogen (N)-containing compound including proteins are significantly different from part to part of the root. In other words, the main root contains less ginsenosides than other lateral or fine roots. Contents of polysaccharides and N-containing compound in main root is higher. However, the quality control of ginseng products focused on non-saponin compounds has limitation in applying to the analytical method, because of the difficult chemical analysis of these compounds. Content of ginsenosides, and ratios of PD/PT and ginsenoside Rb,/Rg, are inversely proportional to the diameter of ginseng root. Therefore, these can be served as the chemical parameters for the indirect method of evaluating from what part of the root does the material originate. Furthermore, contents of polysaccharides and N-containing compounds show inverse relationship to saponin content. Therefore, it seems that index for analytical chemistry of saponin can be applied to the indirect method of evaluating not only saponin but also non-saponin compounds of ginseng. From these viewpoints, it is strongly recommended that quality of ginseng or ginseng products be judged not only by the absolute content of given ginsenoside but also by varieties and compositional balance of ginsenosides, including contents of non-saponin active principles.

• Management & Information Systems Review
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• v.30 no.2
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• pp.211-235
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• 2011

We examined the relationship between innovation capability of R&D and the firm's performance by mainly comparing regional strategy industry with non-regional strategy industry. Also, this analysis involved comparing the relationship by regional strategy industry. For the purpose of this study, we divided innovation capability of R&D into input, process and output. The first of main results in this study was that regional strategic industry was significantly higher than non-regional strategy industry in innovation capability of R&D with the exception of the CEO's mind for technological innovations. However, we found no significant difference in the firm's performance. Second, in the results of comparing innovation capability of R&D and the firm's performance by regional strategy industry, electronic-information equipment industry was significantly superior to other industries. Third, it was found that the relationship between innovation capability of R&D and the firm's performance was different by regional strategy industry. Also, R&D manpower and R&D process were more significant factors affecting the firm's performance rather than R&D input and output.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5104-5113
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• 2012

To study the influence of leadership types on achievement goal orientation of high school athletes, Multifactor Leadership Questionnaire (MLQ) and Task and Ego Orientation in Sport Questionnaire (TEOSQ) were used to two-hundred-ninety athletes in Physical Education High Schools. The data from the survey were analyzed by SPSS 19.0 and the results of the analysis were as in the following. First, there were meaningful differences in the leadership factors according to the demographic characteristics. Male athletes tended to be more conscious of the individualized consideration(transformation leadership), contingent reward, management by exception(transactional leadership) of the leadership factors. Second, there were differences in athletes' achievement goal orientation. Comparing to the female athletes, male athletes gave more importance on the task goal orientation. Third, there were differences in the leader's leadership types on the athlete's achievement goal orientation. The athletes with self goal orientation and the individualized consideration (transformational leadership) of leadership types showed statistically meaningful differences. The athletes with high self goal orientation had a negative relationship to the individualized consideration and a positive relationship to the intellectual stimulation. The athletes with task goal orientation showed differences in the transactional leadership as well as in the contingent reward.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.4
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• pp.353-361
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• 1990

A number of correlation and regression analyses were performed on data from apparent and true digestibility of amino acids at ileal and fecal level with finishing pigs, in order to investigate whether these amino acid digestibilities could be predicted with certainly degree on their fiber fractions content (chemical analysis). The data comprised 16 diets varying in 4 levels of crude fiber and from 4 fiber sources. The relationships between fiber fractions including crude fiber (CF), NDF, ADF lignin and cellulose contents on apparent and true digestibility of almost all amino acids in both ileal and fecal level were negative, except glutamine at fecal level. In apparent digestibility at ileal level, the correlations of fiber fractions were moderate (r of NDF = 0.53 to 0.63; ADF, 0.50 to 0.77; cellulose, 0.50 to 0.75), with an exception of CF content was relatively high (r of 0.58 to 0.81). The correlations to true digestibility of amino acids were weaker. In case of at fecal level, the higher correlation (negative) was found with NDF than CF content. Estimations of amino acids digestibility were performed using regression equation. The data showed that apparent digestibility of amino acids could be estimated for almost amino acids except arginine, threonine, valine and tyrosine at fecal level and phenylalaine, valine and glycine at ileal level. The best prediction at ileal and fecal level ($r^2=0.55-0.77$ and 0.52-0.76), respectively was obtained with NDF content. Prediction for true digestibility of amino acids, none of fiber fractions could be estimated for arginine, leucine and valine at all collection levels. At ileal level, CF could be used for most of amino acids except phenylalanine, glycine and praline; cellulose, only for lysine and methionine and NDF, only for proline. At fecal level, glutamine digestibility could be estimated only from CF and ADF content. The best predictor at ileal level was ADF content whereas at fecal level was NDF content. These results indicate that lignin content could not be used as predictor for all amino acids at both levels neither in apparent nor true digestibility.

• Journal of Digital Convergence
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• v.13 no.8
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• pp.345-356
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• 2015

Health service quality is increasingly emphasized, and primary care service is no exception in this. This study identified patients' perception of quality considering convergence factors associated with increased utilization among patients over the age of 65 using community health centers. A total of 307 respondents were included in the analysis. The results showed that positive perceptions on facility and environment, patient-centered care, technical knowledge and skills of physicians, and emotional support with nurses and non-medical workers were statistically and significantly associated with higher utilization. In order to increase utilization of primary care service at community health centers, they need to manage and improve these factors.