• Title/Summary/Keyword: exact sequence

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Retreatability of two endodontic sealers, EndoSequence BC Sealer and AH Plus: a micro-computed tomographic comparison

  • Oltra, Enrique;Cox, Timothy C.;LaCourse, Matthew R.;Johnson, James D.;Paranjpe, Avina
    • Restorative Dentistry and Endodontics
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    • v.42 no.1
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    • pp.19-26
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    • 2017
  • Objectives: Recently, bioceramic sealers like EndoSequence BC Sealer (BC Sealer) have been introduced and are being used in endodontic practice. However, this sealer has limited research related to its retreatability. Hence, the aim of this study was to evaluate the retreatability of two sealers, BC Sealer as compared with AH Plus using micro-computed tomographic (micro-CT) analysis. Materials and Methods: Fifty-six extracted human maxillary incisors were instrumented and randomly divided into 4 groups of 14 teeth: 1A, gutta-percha, AH Plus retreated with chloroform; 1B, gutta-percha, AH Plus retreated without chloroform; 2A, gutta-percha, EndoSequence BC Sealer retreated with chloroform; 2B, gutta-percha, EndoSequence BC Sealer retreated without chloroform. Micro-CT scans were taken before and after obturation and retreatment and analyzed for the volume of residual material. The specimens were longitudinally sectioned and digitized images were taken with the dental operating microscope. Data was analyzed using an ANOVA and a post-hoc Tukey test. Fisher exact tests were performed to analyze the ability to regain patency. Results: There was significantly less residual root canal filling material in the AH Plus groups retreated with chloroform as compared to the others. The BC Sealer samples retreated with chloroform had better results than those retreated without chloroform. Furthermore, patency could be re-established in only 14% of teeth in the BC Sealer without chloroform group. Conclusion: The results of this study demonstrate that the BC Sealer group had significantly more residual filling material than the AH Plus group regardless of whether or not both sealers were retreated with chloroform.

Response of the Wave Spectrum to Turning Winds (풍향 변화에 대한 파랑 스펙트럼의 반응)

  • 윤종태
    • Journal of Ocean Engineering and Technology
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    • v.11 no.4
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    • pp.111-121
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    • 1997
  • The spectral energy balance model is composed and the nonlinear interaction is approximated by the discrete interaction parameterization as in WAM model. The numerical results of durational limited growth test agree very well with those of the exact model, EXACT-NL. The response of a wave spectrum to a change in wind direction is investigated numerically for a sequence of direction changes 30$^{\circ}$ , 45$^{\circ}$ , 60$^{\circ}$ , 90$^{\circ}$ . The high frequency components relax more repidly to the new wind direction than the low frequency components and the relaxation process also depends on the wave age. For wind direction changes less than 60$^{\circ}$ , the coupling by nonlinear interaction is so strong that the secondary peak in input source distribution is counteracted by the negative lobe of the nonlinear interaction. For wind direction changes grater than 60$^{\circ}$ , a second independent wind-sea spectrum is generated in the new wind direction, while the old spectrum gradually decays as swell.

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1H, 15N and 13C resonance assignment and secondary structure prediction of ss-DNA binding protein 12RNP2 precursor, HP0827 from Helicobacter pylori

  • Jang, Sun-Bok;Ma, Chao;Chandan, Pathak Chinar;Kim, Do-Hee;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.15 no.1
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    • pp.69-79
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    • 2011
  • HP0827 has two RNP motif which is a very common protein domain involved in recognition of a wide range of ssRNA/DNA.We acquired 3D NMR spectra of HP0827 which shows well dispersed and homogeneous signals which allows us to assign 98% of all $^1H_N$, $^{15}N$, $^{13}C_{\alpha}$, $^{13}C_{\beta}$ and $^{13}C$=O resonances and 90% of all sidechain resonances. The sequence-specific backbone resonance assignment of HP0827 can be used to gain deeper insights into the nucleic acids binding specificity of HP0827 in the future study. Here, we report secondary structure prediction of HP0827 derived from NMR data. Additionally, ssRNA/DNA binding assay studies was also conducted. This study might provide a clue for exact function of HP0827 based on structure and sequence.

One Step Cloning of Defined DNA Fragments from Large Genomic Clones

  • Scholz, Christian;Doderlein, Gabriele;Simon, Horst H.
    • BMB Reports
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    • v.39 no.4
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    • pp.464-467
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    • 2006
  • Recently, the nucleotide sequences of entire genomes became available. This information combined with older sequencing data discloses the exact chromosomal location of millions of nucleotide markers stored in the databases at NCBI, EMBO or DDBJ. Despite having resolved the intron/exon structures of all described genes within these genomes with a stroke of a pen, the sequencing data opens up other interesting possibilities. For example, the genomic mapping of the end sequences of the human, murine and rat BAC libraries generated at The Institute for Genomic Research (TIGR), reveals now the entire encompassed sequence of the inserts for more than a million of these clones. Since these clones are individually stored, they are now an invaluable source for experiments which depend on genomic DNA. Isolation of smaller fragments from such clones with standard methods is a time consuming process. We describe here a reliable one-step cloning technique to obtain a DNA fragment with a defined size and sequence from larger genomic clones in less than 48 hours using a standard vector with a multiple cloning site, and common restriction enzymes and equipment. The only prerequisites are the sequences of ends of the insert and of the underlying genome.

A Block Matching using the Motion Information of Previous Frame and the Predictor Candidate Point on each Search Region (이전 프레임의 움직임 정보와 탐색 구간별 예측 후보점을 이용하는 블록 정합)

  • 곽성근;위영철;김하진
    • Journal of KIISE:Computing Practices and Letters
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    • v.10 no.3
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    • pp.273-281
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    • 2004
  • There is the temporal correlation of the video sequence between the motion vector of current block and the motion vector of previous block. In this paper, we propose the prediction search algorithm for block matching using the temporal correlation of the video sequence and the center-biased property of motion vectors. The proposed algorithm determines the location of a better starting point for the search of an exact motion vector using the point of the smallest SAD(sum of absolute difference) value by the predicted motion vector from the same block of the previous frame and the predictor candidate point on each search region. Simulation results show that PSNR(Peak-to-Signal Noise Ratio) values are improved up to the 1.06㏈ as depend on the video sequences and improved about 0.19∼0.46㏈ on an average except the full search(FS) algorithm.

Generalized Quaternary Quasi-Orthogonal Sequences Spatial Modulation (일반화한 쿼터너리 준직교 시퀀스 공간변조 기법)

  • Shang, Yulong;Kim, Hojun;Jung, Taejin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.4
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    • pp.404-414
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    • 2016
  • So called quaternary quasi-orthogonal sequence spatial modulation (Q-QOS-SM) has been presented with an advantage of improved throughputs compared to the conventional SM and generalized spatial modulation (GSM) by virtue of a larger set size of QOSs and its minimized correlation value between these QOSs. However the Q-QOS-SM has been originally invented for limited transmit antennas of only powers of two. In this paper, by extending the Q-QOS-SM to any number of transmit antennas, we propose a generalized Q-QOS-SM, referred as G-QO-SM. Unlike the conventional Q-QOS-SM using the Q-QOSs of length of any power of two, the proposed G-QO-SM is constructed based on the Q-QOSs of only the lengths of 2 and 4. The proposed scheme guarantees the transmission of the total $N_t$ spatial bits with $N_t$ transmit antennas, and thus achieves greatly higher throughputs than the other existing schemes including the SM, GSM, Q-QOS-SM, Quadrature-SM, and Enhanced-SM. The performance improvements of the proposed G-QO-SM is justified by comparing the analytically derived BER upper bounds and also the exact Monte Carlo simulation results.

Identification of New Microsatellite Markers in Panax ginseng

  • Kim, Joonki;Jo, Beom Ho;Lee, Kyoung Lyong;Yoon, Eui-Soo;Ryu, Gi Hyung;Chung, Ki Wha
    • Molecules and Cells
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    • v.24 no.1
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    • pp.60-68
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    • 2007
  • Microsatellites, also called simple sequence repeats (SSR), are very useful molecular genetic markers commonly used in crop breeding, species identification and linkage analysis. In the present study, we constructed a microsatellite-enriched genomic library of Panax ginseng, and identified 251 novel microsatellite sequences. Tri-nt repeat units were the most abundant (46.6%), followed by di-nt repeats (35.5%). The $(AG)_n$ motif was most common (23.1%), followed by the $(AAC)_n$ motif (22.3%). From the genotyping of 94 microsatellites using marker-specific primer sets, we identified 11 intraspecific polymorphic markers as well as 14 possible interspecific polymorphic markers differing between P. ginseng and P. quinquefolius. The exact allele structures of the polymorphic markers were determined and the alleles were named. This study represents the first report of the bulk isolation of microsatellites by screening a microsatellite-enriched genomic library in P. ginseng. The microsatellite markers could be useful for linkage analysis, genetic breeding and authentication of Panax species.

Adaptive Motion Vector Estimation Using the Regional Feature (영역별 특성을 이용한 적응적 움직임 벡터 추정 기법)

  • Park, Tae-Hee;Lee, Dong-Wook;Kim, Jae-Min;Kim, Young-Tae
    • Proceedings of the KIEE Conference
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    • 1995.11a
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    • pp.502-504
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    • 1995
  • In video image compression, it is important to extract the exact notion information from image sequence in order to perform the data compression, the field rate conversion, and the motion compensated interpolation effectively. It is well known that the location of the smallest sum of absolute difference(SAD) does not always give the true motion vector(MV) since the MV obtained via full block search is often corrupted by noise. In this paper, we first classifies the input blocks into 3 categories : the background, the shade-motion, and the edge-motion. According to the characteristics of the classified blocks, multiple locations of relatively small SAD are searched with an adaptive search window by using the proposed method. The proposed method picks MVs among those candidates by using temporal correlation. Since temporal correlation reveals the noise level in a particular region of the video image sequence, we are able to reduce the search are very effectively.

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Genetic Diversity and Gene Flow Patterns in Pollicipes mitella in Korea Inferred from Mitochondrial DNA Sequence Analysis

  • Yoon, Moongeun;Jung, Ju-Yeon;Kim, Dong Soo
    • Fisheries and Aquatic Sciences
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    • v.16 no.4
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    • pp.243-251
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    • 2013
  • Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population.

A Shadow Region Suppression Method using Intensity Projection and Converting Energy to Improve the Performance of Probabilistic Background Subtraction (확률기반 배경제거 기법의 향상을 위한 밝기 사영 및 변환에너지 기반 그림자 영역 제거 방법)

  • Hwang, Soon-Min;Kang, Dong-Joong
    • Journal of Institute of Control, Robotics and Systems
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    • v.16 no.1
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    • pp.69-76
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    • 2010
  • The segmentation of moving object in video sequence is a core technique of intelligent image processing system such as video surveillance, traffic monitoring and human tracking. A typical method to segment a moving region from the background is the background subtraction. The steps of background subtraction involve calculating a reference image, subtracting new frame from reference image and then thresholding the subtracted result. One of famous background modeling is Gaussian mixture model (GMM). Even though the method is known efficient and exact, GMM suffers from a problem that includes false pixels in ROI (region of interest), specifically shadow pixels. These false pixels cause fail of the post-processing tasks such as tracking and object recognition. This paper presents a method for removing false pixels included in ROT. First, we subdivide a ROI by using shape characteristics of detected objects. Then, a method is proposed to classify pixels from using histogram characteristic and comparing difference of energy that converts the color value of pixel into grayscale value, in order to estimate whether the pixels belong to moving object area or shadow area. The method is applied to real video sequence and the performance is verified.