• 제목/요약/키워드: ex-situ

검색결과 241건 처리시간 0.029초

다변량 지구과학 데이터와 가우시안 혼합 모델을 이용한 공간 분포 추정 (Estimation of Spatial Distribution Using the Gaussian Mixture Model with Multivariate Geoscience Data)

  • 김호림;유순영;윤성택;김경호;이군택;이정호;허철호;류동우
    • 자원환경지질
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    • 제55권4호
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    • pp.353-366
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    • 2022
  • 지구과학 데이터(지오데이터)의 공간 이질성, 희소성 및 고차원성으로 인해 공간 분포 추정에 어려움이 있다. 따라서 지구과학의 많은 응용 분야에서 지오데이터의 고유 특성을 고려할 수 있는 공간 추정 기법이 필요하다. 본 연구에서는 기계 학습 알고리즘 중 하나인 가우시안 혼합 모델(Gaussian Mixture Model; GMM)을 이용하여 공간 예측 방법을 제공하고자 하였다. 제안된 기법의 성능을 검증하기 위해, 옛 제련소 부지에서 휴대용 X선 형광분석기(PXRF) 및 유도결합플라즈마-원자방출분광법(ICP-AES)을 이용하여 분석된 토양 농도 자료를 활용하였다. ICP-AES를 이용해 분석된 As와 Pb를 주변수로 하고, 나머지 자료는 보조변수로 활용하였다. 다차원의 보조변수 중 중요 변수를 선별하기 위해 랜덤포레스트 기반의 변수선택법을 적용하였다. ICP-AES 및 PXRF를 통해 구축된 다변량 데이터를 사용한 GMM의 결과를 단변량 및 이변량 데이터를 사용한 정규 크리깅(Ordinary Kriging; OK) 및 정규 공동크리깅(Ordinary Co-Kriging; OCK)의 결과와 비교하였다. GMM의 결과는 OK 및 OCK의 결과보다 낮은 평균 제곱근 편차(RMSE; 비소는 최대 0.11 및 납은 0.33까지 향상)와 높은 상관관계(r; 비소는 최대 0.31 및 납은 0.46까지 향상)를 제공하였다. 이는 GMM을 사용할 경우 토양 오염의 범위 해석의 성능을 향상시킬 수 있음을 지시한다. 본 연구는 다 변량 공간추정 접근법이 복잡하고 이질적인 지질 및 지구 화학자료의 특징을 이해하는 데 효과적으로 적용될 수 있음을 증명하였다.

중심 주파수 200 kHz의 과학어군탐지기를 활용한 전갱이의 광대역 주파수 특성 (Ex situ combined in situ target strength of Japanese horse mackerel using a broadband echosounder)

  • 강명희;김한수;강동하;정지훈;Fredrich Simanungkalit;강돈혁
    • 수산해양기술연구
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    • 제60권2호
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    • pp.142-151
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    • 2024
  • Recently, domestic fishing production of Japanese horse mackerel has been continuously decreasing. To achieve sustainable fishing of this species, it is essential to acquire its target strength (TS) for accurate biomass estimation and to study its ecological characteristics. To date, there has been no TS research using a broadband echosounder targeting Japanese horse mackerel. In this study, for the first time, we synchronized an underwater camera with a broadband frequency (nominal center frequency of 200 kHz, range: 160-260 kHz) to measure the TS according to the body size (16.8-35.5 cm) and swimming angle of the species. The relationship between Japanese horse mackerel length and body weight showed a general tendency for body weight to increase as length increased. The pattern of the frequency spectra (average values) by body length exhibited a similar trend regardless of body length, with no significant fluctuations in frequency observed. The lowest TS value was observed at 243 kHz while the highest TS values were recorded at 180 and 257.5 kHz. The frequency spectra for the swimming angles appeared to be flat at angles of -5, 0, 30, 60, 75, and 80° while detecting more general trends of frequency spectra for swimming angle proved challenging. The results of this study can serve as fundamental data for Japanese horse mackerel biomass estimation and ecological research.

Cryopreservation of Mulberry Germplasm Core Collection and Assessment of Genetic Stability through ISSR Markers

  • Rao, A. Ananda;Chaudhury, Rekha;Kumar, Suseel;Velu, D.;Saraswat, R.P.;Kamble, C.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제15권1호
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    • pp.23-33
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    • 2007
  • A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

보호종인 히 어 리의 자생지 내외 보전과 지역사회 협력 모델 개발 II. 순천(전남) 청소골 지역 히어리개체군의 분포특성 및 동태 (Conservation of an Endangered CofEopsis coreana Uyeki in and ex situ and Development of II. Dynamics and Distribution Characteristics of the Coryzopsis coreana Population at Cheongsogol, Sunchon, Cholanam-do, Korea)

  • 임동옥;정흥락;김종홍;황인천;김철환;이현우
    • 한국환경생태학회지
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    • 제19권3호
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    • pp.269-278
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    • 2005
  • 전라남도 순천시 서 면 청소골 일대에 분포하는 히어 리개체군의 분포특성과 개체군 동태를 조사하였다. 히어리개체군은 계곡부와 인접한산림 가장자리를 따라 대상으로 분포하였으며,북사면,북서사면 및 북동사면에만 분포하는 특이성을 보였다. 교목층이 형성된 삼림내에서 아교목층이나 관목층을 이루고 있으며,종조성에 있어서는 히어리가 분포하지 않는 인접지 역 삼림식생과 별다른 차이는 없었다. $25\;m^2$당 평균개체수와 개체당 평균 맹아지는 각각 6.25주 및 3개로서 다른 지 역 에 비해 낮은 밀도를 나타냈으며, 이 지 역의 히어 리개체군이 쇠퇴해가고 있음을 알 수 있었다.

$\beta$-시클로덱스트린($\beta$-Cyclodextrin)의 결합 특성과 벤젠의 생물학적 분해에의 적용에 대한 연구 (A Study on the Binding Characteristics of $\beta$-Cyclodextrin with Benzene and Its Application on the Bioremediation)

  • 최종규;손현석;조경덕
    • 한국환경보건학회지
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    • 제28권5호
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    • pp.65-70
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    • 2002
  • Recently, surfactants were frequently used in order to desorb the hydrophobic organic compounds (HOCs) from soil and to enhance the bioavailability. Among them, -cyclodextrin ($\beta$-CD) is one of those. This study was performed to investigate the binding characteristics between benzene and $\beta$-CD and to examine the bioavailability of benzene. First, we investigated binding characteristics between benzene and $\beta$-CD in water and water/soil system. Then, we examined the effect of $\beta$-CD on the biodegradation of benzene in water and water/soil system. Experimental results on the binding characteristics showed that $\beta$-CD resulted in an efficient complex formation with benzene. As -CD concentration increased, the benzene concentration complexed with $\beta$-CD rapidly increased to 30-40% initial benzene added, and reached the equilibrium. We also investigated the effect of $\beta$-CD on the desorption of benzene from soil in the water/soil system. As $\beta$-CD concentration increased, benzene concentration desorbed into water increased up to 90%. How-ever, in its application to biodegradation of benzene in water and water/soil system, the biodegradation rate of benzene did not improved in the presence of $\beta$-CD compared with in the absense of $\beta$-CD. This result indicated that $\beta$-CD was more preferentially used as a carbon source than benzene. Therefore, for remediation of benzene contaminated soils, $\beta$-CD can be used as a surfactant to desert benzene from soil, and then ex-situ chemical treatment can be applied for the remediation.

TRPHs - 중금속 복합오염토양의 동시 처리를 위한 과황산 산화 - 구연산 세척 혼성공정 개발 (A Continuous Process of Persulfate Oxidation and Citric acid Washing for the Treatment of Complex-Contaminated Soil Containing Total Recoverable Petroleum Hydrocarbons and Heavy Metals)

  • 윤나경;최지연;신원식
    • 한국환경과학회지
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    • 제27권1호
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    • pp.1-10
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    • 2018
  • A continuous process of persulfate oxidation and citric acid washing was investigated for ex-situ remediation of complex contaminated soil containing total recoverable petroleum hydrocarbons (TRPHs) and heavy metals (Cu, Pb, and Zn). The batch experiment results showed that TRPHs could be degraded by $Fe^{2+}$ activated persulfate oxidation and that heavy metals could be removed by washing with citric acid. For efficient remediation of the complex contaminated soil, two-stage and three-stage processes were evaluated. Removal efficiency of the two-stage process (persulfate oxidation - citric acid washing) was 83% for TRPHs and 49%, 53%, 24% for Cu, Zn, and Pb, respectively. To improve the removal efficiency, a three-stage process was also tested; case A) water washing - persulfate oxidation - citirc acid washing and case B) persulfate oxidation - citric acid washing (1) - citric acid washing (2). In case A, 63% of TRPHs, 73% of Cu, 60% of Zn, and 55% of Pb were removed, while the removal efficiencies of TRPHs, Cu, Pb, and Zn were 24%, 68%, 62%, and 59% in case B, respectively. The results indicated that case A was better than case B. The three-stage process was more effective than the two-stage process for the remediation of complex-contaminated soil in therms of overall removal efficiency.

남해 연안에 분포하는 해파리(Aurelia aurita, Cyanea nozakii)의 복수 개체에 의한 음향 표적강도 특성 (Acoustic Target Strength Characteristics of Two Species of Multiple Jellyfishes, Aurelia aurita and Cyanea nozakii, in the Southern Coast of Korea)

  • 강돈혁;김정훈;임선호
    • Ocean and Polar Research
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    • 제32권2호
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    • pp.113-122
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    • 2010
  • Understanding the abundance and distribution of massive jellyfish is necessary to forecast where or when their blooms will happen. The acoustic technique is one of the most useful methods of obtaining information if the acoustic characteristics of the targets are known. This study was conducted to determine the acoustic target strength (TS, dB) of two jellyfish species, Aurelia aurita and Cyanea nozakii, in the southern coast of Korea. For the ex situ measurements, 120, 200, and 420 kHz split beam transducers were used, and jellyfish with various bell lengths were arranged to prepare multiple jellyfish. Under 2 vertical individuals, the mean TS for multiple A. aurita at 120, 200, and 420 kHz was -72.7, -71.7, and -68.2 dB, respectively. In the case of 5 vertical individuals, the mean TS of the species was -71.3, -68.2, and -62.0 dB. Finally, the mean TS of C. nozakii at 120, 200, and 200 kHz was -62.0, -60.3, and -58.2 dB under 2 individuals and -58.1, -57.4, and -54.0 dB under 4 individuals, respectively. For both species, higher numbers of jellyfish resulted in a higher TS. In addition, higher frequencies were associated with a higher TS for the same jellyfish. These TS results for two species can be used as essential data for the acoustic detection of jellyfish in an open ocean or coastal area.

Al 분말의 수화 반응과 스파크 플라즈마 열처리법으로 제조된 알루미나 성형체 연구 (Study of Hydrolysis of Al Powder and Compaction of Nano Alumina by Spark Plasma Sintering(SPS))

  • 엄영랑;이민구;이창규
    • 한국분말재료학회지
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    • 제12권6호
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    • pp.422-427
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    • 2005
  • The $Al_2O_3$ with various phases were prepared by simple ex-situ hydrolysis and spark plasma sintering (SPS) process of Al powder. The nano bayerite $(\beta-Al(OH)_3)$ phase was derived by hydrolysis of commercial powder of Al with micrometer size, whereas the bohemite (AlO(OH)) phase was obtained by hydrolysis of nano Al powder synthesized by pulsed wire evaporation (PWE) method. Compaction as well as dehydration of both nano bayerite and bohemite was carried out simultaneously by SPS method, which is used to fabricate dense powder compacts with a rapid heating rate of $100^{\circ}C$ per min. under the pressure of 50MPa. After compaction treatment in the temperature ranges from $100^{\circ}C\;to\; 1100^{\circ}C$, the bayerite and bohemite phases change into various alumina phases depending on the compaction temperatures. The bayerite shows phase transition of $Al(OH)_3{\to}{\eta}-Al_2O_3{\to}{\theta}-Al_2O_3{\to}\alpha-Al_2O_3$ sequences. On the other hand, the bohemite experiences the phase transition from AlO(OH) to ${\gamma}-Al_2O_3\;at\;350^{\circ}C.$ It shows AlO(OH) ${\gamma}-Al_2O_3{\to}{\delta}-Al_2O_3{\to}{\alpha}-Al_2O_3$ sequences. The ${\gamma}-Al_2O_3$ compacted at $550^{\circ}C$ shows a high surface area $(138m^2/g)$.

Side-Aspect Target Strength Measurement of Swimbladdered Fish Using Multi-Frequencies System: Black Sea Bream (Acanthopagrus schlegeli)

  • Cho, Sung-Ho;Kang, Don-Hyug;Na, Jung-Yul;Myoung, Jung-Goo;Kim, Jong-Man
    • The Journal of the Acoustical Society of Korea
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    • 제25권3E호
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    • pp.101-109
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    • 2006
  • Besides dorsal-aspect target strength (TS) of the fish, side-aspect TS information is also acoustically important parameter in fisheries acoustics. In this study, the side-aspect TS of 11 black sea bream (Acanthopagurus schlegeli) were measured using a split beam echosounder of 120, 200, and 420 kHz; total length of the fish ranged from 12.4 to 23.7 cm, and wet weight from 27.5 to 229.8g. For the precise TS measurement with side-aspect angle, we used anesthetized and tethered specimens of known size while being rotated through $360^{\circ}C$ by means of a carousel structure. The side-aspect TS measurements of the fish were conducted by rotating the fish in the horizontal plane at 50 interval. The ping interval was 0.2 second and elapsed time at each angle was 30-60 second. As a result, the measured side-aspect TS data were fitted by sinusoidal function. The relationships between fish length and near full side-aspect TS were as follows: $TS_{120kHz}= 21.46 log (TL)-67.5 (r = 0.70), \;TS_{200 kHz}= 31.03 log (TL)-76.9 (r=0.83),\;TS_{420 kHz}= 30.79 log (TL)-72.2 (r = 0.77)$. For comparison, theoretically estimated side-aspect TS from the Kirchhoff ray mode (KRM) model, which based on swimbladder and body morphology, were compared with the measured TS.

조팝나무의 유전적 다양성과 집단구조 분석을 위한 ISSR 분석 (Genetic Diversity and Population Structure of Spiraea prunifolia for. simpliciflora by Inter-Simple Sequence Repeats)

  • 허만규
    • 생명과학회지
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    • 제19권9호
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    • pp.1183-1189
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    • 2009
  • 조팝나무는 목본이며 약용으로 매우 중요하며 우리나라 산림청 지정 보호수종이다. 이 속내 7집단에서 85개체에 대해 ISSR (inter simple sequence repeats) 마커로 이들 집단에 대한 유전적 변이와 집단구조를 조사하였다. 65개의 다형성 좌위와 78개 ISSR 유전자형을 얻었다. 덕유산 집단과 능동산 집단에는 1개체 이상 공유하는 유전자형이 포함되어 있었다. 전체 유전적 다양도는 종수준과 집단수준에서 각각 0.293과 0.183이였다. 집단의 분화($G_{ST}$)는 0.373으로 나타났다. 따라서 전체 변이의 37.3%는 집단 간에 있었다. ISSR 마커로 한국 내 조팝나무 집단의 분화는 잘 분리되어 ISSR로 조팝나무 집단 연구에 유익하며 유전적 다양도와 집단구조의 통찰은 종보전에 대한 기초 정보로 활용할 수 있을 것으로 사료된다.