• The Plant Pathology Journal
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• v.30 no.2
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• pp.151-158
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• 2014

Three double-stranded RNAs (dsRNAs), approximately 1.85, 1.65 and 1.27 kb in size, were detected in an isolate of Cytospora sacchari from Iran. Partial nucleotide sequence revealed a 1,284 bp segment containing one ORF that potentially encodes a 405 aa protein. This protein contains conserved motifs related to RNA dependent RNA polymerases (RdRp) that showed similarity to RdRps of partitiviruses. The results indicate that these dsRNAs represent a novel Partitivirus that we tentatively designate Cytospora sacchari partitivirus (CsPV). Treatment of the fungal strain by cyclohexamide and also hyphal tip culture had no effect on removing the putative virus. Phylogenetic analysis of putative RdRp of CsPV and other partitiviruses places CsPV as a member of the genus Partitivirus in the family Partitiviridae, and clustering with Aspergillus ochraceous virus 1.

• Animal Systematics, Evolution and Diversity
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• v.6 no.1
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• pp.1-16
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• 1990

The skull and superficial cephalis muscles of Coreoleuciscus splendidus Mori was investigated comparatively with other some fishes of the family Cyprinidae to study on the systematic position of this species. Coreoleuciscs splendidus was unique in the morphology of sphenotic process and the urohyal and this species differ from the true bottom dwellers of the subfamily Gobioninae in the strucutre of cephalic lateral-line canals, the relation of parasphenoids with pterotic and prootic, the morphology of urohyal , basihyal , hypobranchial, and , particulary, the configuration of adductor manibulae complex. With reference to the information so far obtained , the taxonomic position of the present species was discussed in the Cyprinidae.

• Animal Systematics, Evolution and Diversity
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• v.30 no.3
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• pp.183-190
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• 2014

The four species of the family Dendrophylliidae are newly recorded in Korea: Balanophyllia (Balanophyllia) cumingii Milne Edwards and Haime, 1848, Balanophyllia (Balanophyllia) vanderhorsti Cairns, 2001, Cladopsammia eguchii (Wells, 1982), and Cladopsammia gracilis (Milne Edwards and Haime, 1848). The two genera of Balanophyllia and Cladopsammia, to which the four species belong, are newly recorded in Korea. They were collected from the subtidal zones in Jeju-do Island, Korea by SCUBA diving from 1987 to 2012. This study aims to identify the four dendrophyllid species based on external and internal morphological characters including growth form, size, budding, and color of colonies, shape and size of corallites, columella, theca, and septa. Balanophyllia (Balanophyllia) cumingii is distinguished by its solitary growth form, small and low subturbinate corallite with enlarged calice, and expanded basal part, exsert first and second septa, and Pourtal$\acute{e}$s plan. Balanophyllia (Balanophyllia) vanderhorsti is characterized by its quasi-colonial growth form, subturbinate corallites with compressed calice, thick theca, and Pourtal$\acute{e}$s plan. Cladopsammia eguchii is characterized by its phaceloid growth form of compressed corallites basally united with common coenosteum, flat spongy columella, thick theca, and Pourtal$\acute{e}$s plan. Cladopsammia gracilis is distinguished by its phaceloid growth form of corallites basally united with common coenosteum, and pronounced Pourtal$\acute{e}$s plan forming flower patterns.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.237-242
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• 2007

Genome duplication(i.e. polyploidy) is a common phenomenon in the evolution of plants. The objective of this study was to achieve a comprehensive understanding of genome duplication for SNP discovery by Thymine/Adenine(TA) cloning for confirmation. Primer pairs were designed from 793 EST contigs expressed in the roots of a supernodulating soybean mutant and screened between 'Pureunkong' and 'Jinpumkong 2' by direct sequencing. Almost 27% of the primer sets were failed to obtain sequence data due to multiple bands on agarose gel or poor quality sequence data from a single band. TA cloning was able to identify duplicate genes and the paralogous sequences were coincident with the nonspecific peaks in direct sequencing. Our study confirmed that heterogeneous products by the co-amplification of a gene family member were the main cause of obtaining multiple bands or poor quality sequence data in direct sequencing. Counts of amplified bands on agarose gel and peaks of sequencing trace suggested that almost 27% of nonrepetitive soybean sequences were present in as many as four copies with an average of 2.33 duplications per segment. Copy numbers would be underestimated because of the presence of long intron between primer binding sites or mutation on priming site. Also, the copy numbers were not accurately estimated due to deletion or tandem duplication in the entire soybean genome.

• Asian review of World Histories
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• v.1 no.1
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• pp.45-84
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• 2013

Empirical research into Đại Việt before the $14^{th}$ century has made little progress since the 1990s. To improve this situation, I here examine how the L$\acute{y}$ dynasty (1009-1226), the first long-lasting dynasty of Đại Việt, established stable local ruleafter the "Tang-Song Transition" in China that changed the entire picture of East Asia (including both Southeast and Northeast Asia). This paper focuses on two issues. First are the local administrative units and their governors. The nature of both higher units like lộ(circuits), phủ and ch$\hat{a}$u (provinces), and basic units like hươg and gi$\acute{a}$p (districts?) will be examined. Second, I examine non-institutional channels of local rule by the imperial family. By combining such administrative and non-administrative means, the L$\acute{y}$ central court enforced a considerably stable local rule for two centuries. Finally, I attempt some preliminary comparisons with the local rule of Goryeo (918-1392) in the Korean peninsula, a polity that shared many features with Đại Việt in the process of localization of the Tang and Song models. I hope this approach of viewing small empires from the standpoint not of their "goal" (modern states) but of their "start" (charter polities), will enrich the discussion of East Asian small empires.

• The Research Journal of the Costume Culture
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• v.17 no.6
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• pp.1034-1046
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• 2009

Based upon literature survey, this research aims to analyze western children's clothing and characteristics over centuries. It was not until the 18th century that children were given serious consideration and that fabrication of clothing began to be designed only for children. The very first clothes which revolutionized children's wear was 'a la matelot' in the 18th century which freed children from physical restriction, recognized gender difference, and sailor suit became popular among all sexes and adopted as school uniform. And then children's clothing was shortened in length and adopted tubular silhouette, which allowed greater comfort and movement. The 20th century saw children's wear reform to be carried out after the World War II due to invention of synthetic fiber, easy care dress material, mass production system and sophistication of marketing methods. Further evidence of improvement of children's social status can be found in contemporary designs: for example, 'casualized' wear, 'character design' which reflects children's psychology and 'family look' which is designed to be worn with mothers. This evolution of children's clothing within western dressing support the view that children's clothing is not a miniature of adult wear but it reflects idiosyncrasies of the era and exerts children's dignity.

• Mycobiology
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• v.46 no.3
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• pp.192-204
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• 2018

The name Golovinomyces cynoglossi s. lat. is traditionally applied to a complex of morphologically similar powdery mildews on hosts of the plant family Boraginaceae. The current species-level taxonomy within this complex is ambiguous due to the lack of phylogenetic examinations. The present study applied phylogenetic methods to clarify the taxonomy of G. cynoglossi s. lat. Phylogenetic analysis of rDNA ITS sequences retrieved from Asian, European and North American specimens revealed that G. cynoglossi s. lat. collections from different hosts involved several species in five clearly separated lineages. Clade I consists primarily of Golovinomyces cynoglossi s. str. on Cynoglossum. Clade III consists of Golovinomyces sequences retrieved from the host genera Symphytum and Pulmonaria. The taxa within clade III are now assigned to G. asperifoliorum comb. nov. Clade V encompasses G. cynoglossi s. lat. on the host genera Bothriospermum, Buglossoides, Echium, Myosotis, and Trigonotis. The taxa within clade V are now assigned to G. asperifolii comb. nov. The species concerned in this study were lecto- and epitypified to stabilize their nomenclature.

• Animal cells and systems
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• v.6 no.2
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• pp.167-170
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• 2002

The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99％) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.

• Clinical and Experimental Pediatrics
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• v.63 no.6
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• pp.195-202
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• 2020

Developments in next-generation sequencing (NGS) techogies have assisted in clarifying the diagnosis and treatment of developmental delay/intellectual disability (DD/ID) via molecular genetic testing. Advances in DNA sequencing technology have not only allowed the evolution of targeted panels but also, and more currently enabled genome-wide analyses to progress from research era to clinical practice. Broad acceptance of accuracy-guided targeted gene panel, whole-exome sequencing (WES), and whole-genome sequencing (WGS) for DD/ID need prospective analyses of the increasing cost-effectiveness versus conventional genetic testing. Choosing the appropriate sequencing method requires individual planning. Data are required to guide best-practice recommendations for genomic testing, regarding various clinical phenotypes in an etiologic approach. Targeted panel testing may be recommended as a firsttier testing approach for children with DD/ID. Family-based trio testing by WES/WGS can be used as a second test for DD/ID in undiagnosed children who previously tested negative on a targeted panel. The role of NGS in molecular diagnostics, treatment, prediction of prognosis will continue to increase further in the coming years. Given the rapid pace of changes in the past 10 years, all medical providers should be aware of the changes in the transformative genetics field.

• Animal cells and systems
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• v.3 no.1
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• pp.1-7
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• 1999

Gonadotropin-releasing hormone (GnRH) was originally isolated as a hypothalamic peptide that regulates reproduction by stimulating the release of gonadotropins. Using comparative animal models has led to the discovery that GnRH has a more ancient evolutionary origin. Durinq evolution GnRH peptide underwent gene duplication and structural changes to give rise to multiple molecular forms of GnRHs. Mammalian GnRH initially considered to be the sole molecular form, is now grouped as a family of peptides along with GnRH variants determined from representatives in all classes of vertebrates. Vertebrate species including primates and humanshave more than one GnRH variant in individual brains; a unique GnRH form in the forebrain and chicken IIGnRH in the midbrain. Furthermore, several species of bony fish have three molecular variants of GnRH: salmon GnRH sea-bream GnRH and chicken II GnRH. Also, it has been shown that in addition to the olfactory placodes and the midbrain, there is a third embryonic source of GnRH neurons from the basal diencephalon in birds and fish, which might be true for other vertebrates. Therefore, comparative animal models like fish with discrete sites of expression of three molecular variants of GnRH in individual brains, could provide insight into novel functions of GnRH variants, conservation of gene regulation, and mechanisms governing reproduction in vertebrates.