• Journal of Microbiology and Biotechnology
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• v.21 no.11
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• pp.1193-1198
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• 2011

Encapsulation of biological material in the permiselective membrane allows to construct a system separating cells from their products, which may find biotechnological as well as biomedical applications in biological processes regulation. Application of a permiselective membrane allows avoiding an attack of the implanted microorganisms on the host. Our aim was to evaluate the performance of Bacillus subtilis encapsulated in an elaborate membrane system producing listeriolysin O, a cytolysin from Listeria monocytogenes, with chosen eukaryotic cells for future application in anticancer treatment. The system of encapsulating in membrane live Bacillus subtilis BR1-S secreting listeriolysin O was proven to exert the effective cytotoxic activity on eukaryotic cells. Interestingly, listeriolysin O showed selective cytotoxic activity on eukaryotic cells: more human leukemia Jurkat T cells were killed than human chronic lymphocytic B cells leukemia at similar conditions in vitro. This system of encapsulated B. subtilis, continuously releasing bacterial products, may affect selectively different types of cells and may have future application in local anticancer treatment.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.3
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• pp.160-180
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• 2012

We have studied the eukaryotic plankton species diversity to compare the community structure of fresh and brackish waters in the lower reaches of the Nakdong River using metagenomic methods. We constructed 18S rDNA clone libraries of total DNAs extracted from environmental water samples collected at Mulgeum (MG100929, fresh) and Eulsukdo bridge (ES, brackish). Through the steps of colony PCR, PCR-RFLP, sequencing and similarity analysis, we discovered the diverse species composition of eukaryotic plankton. Total 338 clones (170 at MG100929 and 168 at ES) were analyzed, and then we found 74 phylotypes (49 for MG100929 and 25 for ES). From the phylogenetic analysis, we confirmed various eukaryotic plankton of broad range of taxonomic groups, including Stramenopiles, Cryptophyta, Viridiplantae, Alveolata, Rhizaria, Metazoa, and Fungi. We also found several unreported species in Korea and candidates of new taxonomic entities at levels higher than genus. Especially, the cryptic species diversity including unreported phylotypes of Pirsonia (Stramenopiles) and Perkinsea (Alveolata) suggests that the molecular monitoring method can produce new informative biological data in monitoring the changes in the Nakdong River Mouth ecosystem.

• Proceedings of the Korean Nutrition Society Conference
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• 1995.11b
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• pp.10-10
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• 1995

• 한국당과학회:학술대회논문집
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• 2006.11a
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• pp.3-5
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• 2006

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.23-23
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• 2015

Plasmodiophora brassicae is a major disease threat for Brassica oil and vegetable crop production worldwide. The causal agent is a Plasmodiophorid, which are obligate biotrophic plant-pathogenic protists in the Rhizarian kingdom. Although the Plasmodiophorids include other important agricultural pathogens such as Polymyxa betae, Spongospora subterranea, their biology remains poorly understood due to their intracellular biotrophic life style. I will present the assembled and annotated genome of P. brassicae, with insights into developmental stage-specific. We provide the first genomic data for pathogenic Rhizaria. The exploitation of the life stage specific transcripts will shed light in the understanding of the life cycle at a molecular basis, which will in the long run help to understand and control club root disease. Our data also fill an important gap for the understanding of the eukaryotic tree of life, since this is only the third genome of the eukaryotic kingdom of Rhizaria.

• BMB Reports
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• v.43 no.5
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• pp.319-324
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• 2010

Experimental bioinformatics data obtained from an E. coli cell-based eukaryotic protein purification experiment were analyzed in order to identify any bottleneck as well as the factors affecting the target purification. All targets were expressed as His-tagged maltose-binding protein (MBP) fusion constructs and were initially purified by immobilized metal affinity chromatography (IMAC). The targets were subsequently separated from the His-tagged MBP through TEV protease cleavage followed by a second IMAC isolation. Of the 743 total purification trials, 342 yielded more than 3 mg of target proteins for structural studies. The major reason for failure of target purification was poor TEV proteolysis. The overall success rate for target purification decreased linearly as cysteine content or isoelectric point (pI) of the target increased. This pattern of pI versus overall success rate strongly suggests that pI should be incorporated into target scoring criteria with a threshold value.

• BMB Reports
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• v.34 no.2
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• pp.118-122
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• 2001

The translocation of ribose-binding protein (RBP) into the inverted membrane vesicles (IMV) of Escherichia coli and eukaryotic microsomes was studied using the in vitro translation/translocation system. It was found that RBP was translocated into heterologous eukaryotic microsomes co-translationally, as well as post-translationally However, RBP was translocated only past-translationally into IMV. Degradation fragments of RBP with the molar mass of 14 and 16 kDa were produced during the translocation into IMV However, the amount of the degradation products decreased and the mature form of RBP appeared in the presence of phenylmethylsulfonyl fluoride (PMSF). PMSF and GTP accelerated the translocation of RBF It was also found that SecB enhanced the post-translational translocation of RBP It appears that RBP is translocated via at least two targeting paths.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.200-209
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• 2003

주어진 염기서열에서 유전자 영역을 예측하는 유전자 구조 예측은 유전체 프로젝트의 중요한 과정 중 하나이며 유전체 프로젝트 전체에 큰 영향을 준다. 진핵생물의 유전체가 원핵생물의 유전체에 비해 더 복잡한 구조를 가지기 때문에 진핵생물의 유전자 구조 예측 모델 역시원핵생물에 비해 다양한 모델이 제안되었다. 본 연구팀은 duration hidden markov model을 기본형태로 하여 EGSP(Eukaryotic Gene Structure Prediction)프로그램을 개발하였다. 현재 개발된 진핵생물의 유전자 구조 예측 알고리즘 중에서 GenScan이 가장 정교한 젓으로 보고 되고 있는데, EGSP의 결과분석을 위해 Genscan과 함께 GeneID, Morgan의 예측결과를 여러 가지 기준에서 비교하였다. EGSP는 정교한 예측모델을 가지고 있음에도 각 구성모듈에 대한 파라메터의 정교함에서 부족한 면이 나타나므로, 모델의 개선과 각 모듈의 조율을 통해 더욱 개선된 결과를 가지게 될 것이다.

• BMB Reports
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• v.30 no.1
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• pp.80-84
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• 1997

The synthesis and secretion of human angiogenin in E. coli by the natural leader sequence has been studied. We constructed a recombinant plasmid containing human angiogenin cDNA which encompassed all the coding region including leader sequence required for secretion. The recombinant plasmid was introduced into a suitable E. coli host. The angiogenin was detected in the culture medium and periplasm upon the induction of gene expression. The molecular weight of the secreted angiogenin was identical to that of authentic angiogenin purfied from human plasma when estimated by SDS-PAGE and immunoblotting. showing that the natural leader sequence was recognized and processed by the secretion machinery of E. coli. The angiogenin concentration in the culture medium reached a maximum within 2 h when expressed at $37^{\circ}C$ with 0.02~2 mM IPTG. In contrast, the expression level increased gradually over time up to 11 h at $23^{\circ}C$ with 0.002~2 mM IPTG and at $37^{\circ}C$ with 0.002 mM IPTG.

• BMB Reports
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• v.50 no.4
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• pp.194-200
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• 2017

Eukaryotic gene expression is precisely regulated at all points between transcription and translation. In this review, we focus on translational control mediated by the 3'-untranslated regions (UTRs) of mRNAs. mRNA 3'-UTRs contain cis-acting elements that function in the regulation of protein translation or mRNA decay. Each RNA binding protein that binds to these cis-acting elements regulates mRNA translation via various mechanisms targeting the mRNA cap structure, the eukaryotic initiation factor 4E (eIF4E)-eIF4G complex, ribosomes, and the poly (A) tail. We also discuss translation-mediated regulation of mRNA fate.