• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.104.2-105
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• 2001

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• Journal of Photoscience
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• 제9권2호
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• pp.94-97
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• 2002

Light is one of the most important environmental factors that influence plant growth and development. It does not function independently but exerts its role through coordinated interactions with intrinsic developmental programs, such as hormonal regulation. One typical example is hypocotyl growth in which light signals are modulated through growth hormones. However, the underlying molecular mechanisms are largely unknown. We demonstrated that brassinosteroids play an important role in the light signal transduction in etiolated hypocotyl growth. A light-responsive Ras-like G-protein, Pra2 from pea, physically and functionally interacts with a cytochrome P450 that specifically catalyzes C-2 hydroxylation in brassinosteroid biosynthesis. The cytochrome P450 expression, along with Pra2, is induced in the dark and predominantly localized in the rapidly elongating zone of etiolated pea epicotyls. Transgenic plants with a reduced level of Pra2 exhibit a dark-specific dwarfism, which is completely rescued by brassinosteroid application. On the contrary, overexpression of the cytochrome P450 results in enhanced hypocotyl growth even in the light, which phenocopies the etiolated hypocotyl growth. It is therefore envisioned that Pra2 is a molecular switch that mediates the crosstalk between light and brassinosteroids in the etiolation process.

• The Plant Pathology Journal
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• 제29권1호
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• pp.93-98
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• 2013

Bacterial wilt is a vascular wilt disease caused by Xanthomonas translucens pv. poae that infects Poa annua, a grass that is commonly found on golf course greens throughout the world. Bacterial wilt causes symptoms of etiolation, wilting, and foliar necrosis. The damage is most prevalent during the summer and the pathogen can kill turf under conditions optimal for disease development. Fifteen isolates of X. translucens pv. poae were collected from northern regions in the United States and tested for virulence against P. annua. All 15 isolates were pathogenic on P. annua, but demonstrated variable levels of virulence when inoculated onto P. annua under greenhouse conditions. The isolates were divided into two virulence groups. The first group containing four isolates generally resulted in less than 40% mortality following inoculation. The second group, containing the other eleven isolates, produced between 90 and 100% mortality following inoculation. These results suggest that differences in the virulence of bacterial populations present on a golf course may result in more or less severe amounts of observed disease.

• 식물조직배양학회지
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• 제28권4호
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• pp.189-195
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• 2001

화경절 액아 유래 신초의 잎을 이용한 팔레높시스 대량번식에서 배양재료로 이용되는 신초의 발생수를 증가시키기 위해 화경배양을 위한 배지, 배양온도 화경 전처리 등 기내 배양조건과 계통 간 화경의 발육형태에 대해 조사하였다. 화경절 액아의 신초발생률은 MS배지에서 90.9%, Hyponex 배지에서 54.4%이었다. 5 mg/L의 BA가 첨가된 MS배지에서 신초가 많이 형성되었으며 발육상태도 양호하였다. 화경 액아의 위치별 발육형태는 기저부 쪽일수록 신초발생이, 상부 쪽일수록 2차화경이 많이 발생되었으며 25~28$^{\circ}C$의 배양온도는 액아로부터 신초를 얻는 데 적절하였으며, 황화처리는 오염률을 낮추는 데 효과가 있었으나 페놀물질 유출량의 감소효과는 없었다. 수집한 30계통 중 27계통의 화경에서 신초를 얻었으며 화경편 당 평균 신초수는 3.17개이었으나 Phal. 3020, 3039 등은 신초가 많이 발생되어 다른 계통과 차이가 있었다. 교잡 F$_1$계통의 화경발육 양상은 모본(수집계통)과 비슷하였다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1994년도 94 Symposium on Plant science September 10, 1994 Ewha Womans University, Seoul, Korea 94 식물학 심포지움 환경 스트레스와 식물의 반응
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• pp.41-51
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• 1994

Tomato (Lycopersicon esculentum Mill.) has been chosen as a model species for the study of hotomorphogenesis. The aurea (au) and yellow-green-2 (yg-2) mutants which are severely photochrome deficient appear to be phytochrome chromophore mutants. Mutants modified with respect to specific members of the phytochrome gene family: the far-red light-insensitive mutant (fri, for phytochrome A) and the temporarily red light-insensitive mutant (tri, for phytochrome B1) have been identified. Mutants that exhibit an exaggerated phytochrome response are putative transduction-chain mutants affecting an amplification step in phytochrome signal transduction. These mutants are being used to understand the complexities of juvenile anthocyanin in the hypocotyl during seedling de-etiolation.

• 한국토양비료학회지
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• 제43권6호
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• pp.975-977
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• 2010

Ammonium nitrogen is volatilized as ammonia at high pH soil. This study was conducted to observe an injury cause of egg plant grown in a high pH rockwool amended with ammonium phosphate. The etiolation symptom (yellowing) was appeared on veins of a leaf but not in healthy root when nutrient solution containing ammonium phosphate in addition to essential elements was applied in a top soil of which pH was 7.8. However, the same symptom did not appeared in the egg plant from the top soil in which the nutrition solution containing potassium phosphate instead of ammonium phosphate was applied. pHs were similar between these two different solutions. This revealed that the injury was caused by ammonia gas.

• Journal of Photoscience
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• 제10권2호
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• pp.189-193
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• 2003

The effects of light on the regulation of ethylene biosynthesis during development of mung bean seedlings were investigated by monitoring the differential expression of seven 1-aminocyclopropane-l-carboxylate (ACC) synthase and two ACC oxidase genes. Among them, only the expression of VR-ACS1, VR-ACS6, VR-ACS7, VR-ACO1 and VR-AC02 was observable in etiolated mung bean hypocotyls. When the seedlings were de-etiolated for 1 d under a light/dark cycle of 16 h/8 h, the expression of VR-ACS6, VR-ACS7 and VR-ACO2 was controlled negatively by light. The expression of VR-ACS1 showed a tendency to increase until 6 h after a dark-to-light transition and then decreased at 12 h. On the other hand, the expression of VR-ACO1 was mostly constitutive up to 12 h after the dark-to-light transition. The opening of hypocotyl hooks during de-etiolation in the light was stimulated by the inhibition of the action of endogenous ethylene in the presence of 1-MCP. These results suggest that the negative regulation of light on the expression of ACC synthase and ACC oxidase genes eventually results in the inhibition of ethylene production with an acceleration of the opening of apical hooks.

• Molecules and Cells
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• 제39권8호
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• pp.581-586
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• 2016

Post-translational modifications (PTMs) of proteins are essential to increase the functional diversity of the proteome. By adding chemical groups to proteins, or degrading entire proteins by phosphorylation, glycosylation, ubiquitination, neddylation, acetylation, lipidation, and proteolysis, the complexity of the proteome increases, and this then influences most biological processes. Although small RNAs are crucial regulatory elements for gene expression in most eukaryotes, PTMs of small RNA microprocessor and RNA silencing components have not been extensively investigated in plants. To date, several studies have shown that the proteolytic regulation of AGOs is important for host-pathogen interactions. DRB4 is regulated by the ubiquitin-proteasome system, and the degradation of HYL1 is modulated by a de-etiolation repressor, COP1, and an unknown cytoplasmic protease. Here, we discuss current findings on the PTMs of microprocessor and RNA silencing components in plants.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2003년도 학술대회지
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• pp.97-103
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• 2003

Phytochrome controls diverse aspects of plant development in response to the ambient light conditions. HFRl, a basic helix-loop-helix protein, is required for a subset of phytochrome A (phy A)-mediated photo-responses in Arabidopsis. Here, we show that overexpression of HFR1-N105, but not the one of the full-length HFR1, confers exaggerated photo-responses. The transgenic plants overexpressing HFR1- N105 exhibited light-independence in a subset of photo-responses, including germination, de-etiolation, gravitropic hypocotyl growth, and blocking of greening. Overexpression of HFR1-N105 also caused constitutive light-responses in the expression of some light-regulated genes. In addition, the HFR1-N105 overexpressor showed hypersensitive responses under R and FR light, dependently on phyB and phyA, respectively. End-of-day far-red light response and petiole elongation were suppressed in the HFR1-N105 overexpressor plants. Together these results imply that overexpression of HFR1-N105 activated a branch of light signaling, supporting the hypothesis that transcriptional regulation in the nucleus would be the primary mechanism of light signaling in Arabidopsis. We discuss the biotechnological potential of the mutant bHLH protein, HFR1-N105 in regard to suppressed shade avoidance syndrome.

• 한국농림기상학회지
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• 제24권3호
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• pp.133-144
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• 2022

본 연구는 UAE 사막환경에서 벼 재배를 위해 재배기간, 적정 벼 유전자원, 생육발달 양상, 물 사용량 등을 조사하고자 수행하였다. UAE 사막환경에서 벼의 재배기간은 UAE 겨울의 저온을 벼의 영양생장기간 동안에 포함하는 11월 하순부터 이듬해 4월 하순까지 이었다. UAE의 기온과 일장에 적응할 수 있는 유전자원을 국내의 인공기상 시설과 간척지 모래토양에서 사전 시험한 결과, 아세미와 FL478이 선정되었다. 사막환경에서 아세미는 생육초기의 황화현상을 극복하여 수확까지 하였으나, FL478은 생육 초기에 황화현상과 생육이 불량하여 고사하였다. UAE 사막환경에서 아세미는 12월 초순부터 이듬해 3월 초순까지 영양생장기, 3월 초순부터 3월 하순까지 생식생장기, 3월 하순부터 4월 하순까지 등숙기의 분포를 보였다. 아세미의 백미수량은 763kg/10a이었고, 한국과 비교하여 약 41.8% 증가한 수량으로 생식생장기와 등숙기의 풍부한 일사량의 영향으로 추정된다. 물 사용량은 UAE 재배기간 동안 2,619 ton/10a 수준으로 한국보다 약 3배 많아 물 절약 기술이 필요한 상황이다. UAE에서 벼 재배는 경제성과 관련이 매우 높기 때문에, 물 절약을 위해서 관개기술, 재배방법 개발 등이 보완되어야 한다. 또한 UAE 사막환경에서 안정적인 벼 재배를 위해서 다양한 적응 유전자원 선정, 생육초기의 황화현상 최소화 방법 등의 추가 연구가 필요할 것으로 판단된다.