• Journal of Life Science
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• v.24 no.5
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• pp.558-566
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• 2014

Growth induced by cadmium (Cd) and ethylsalicylic acid (ESA) and the effect of ESA on rubisco/rubisco activase were studied in tobacco. The effect of denaturants on rubisco/rubisco activase was also investigated. In order to determine optimal concentration of ESA for growth of tobacco, tobacco was treated with $10^{-6}$-10 mM. It was found that its growth was the highest at $10^{-4}$ mM ESA. In the experiment using control, Cd treated group, ESA treated group, and Cd and ESA mixture group, ESA alone showed the highest growth and Cd showed the lowest growth. Cd treated group was the lowest in both rubisco/rubisco activase content and activity. ESA reduced the rubisco/rubisco activase content, but increased their activity. The activity of rubisco was inhibited by treating L-cysteine, urea, thiourea, ${\beta}$-mercaptoethanol, and EDTA other than guanidine-HCl in control group. L-cysteine, urea, thiourea, and guanidine-HCl treatments showed no change, but ${\beta}$-mercaptoethanol and EDTA increased rubisco activase activity. In conclusion, ESA inhibited the content of rubisco and promoted its activity, whereas promoted the content of rubisco activase and inhibited its activity. In addition, the content and activity of rubisco and rubisco activase inhibited by Cd were recovered by ESA. The activity of rubisco and rubisco activase by Cd and ESA was inhibited by the denaturant and the recovery of ESA inhibited by Cd was lost by the denaturant.