• 한국식품과학회지
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• 제27권6호
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• pp.1035-1038
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• 1995

김치의 발효 및 연부, 부패에 관여하는 미생물에 대한 대나무 추출액의 항균성을 측정하기 위하여 김치 초기 숙성균인 Leuconostoc mesenteroides, 김치의 산패에 관여하는 Lactobacillus plantarum, 김치 연부에 관여하는 것으로 알려진 Brettanomyces custersii, Klebsiella oxytoca, Pichia membranaefaciens 등을 사용하여 항균효과를 측정하였다. 먼저, 추출용매의 종류에 따른 대나무잎 추출물의 항균성을 조사하기 위하여, methanol, diethyl ether, acetone, ethyl acetate를 용매로 대나무잎을 추출하였다. 대나무잎 30g을 잘게 잘라서 각각의 용매에 3일간 침지한 후, 농축하여 얻은 추출물을 이용하여 김치발효 미생물에 대한 항균성을 조사하였다. 그 결과, ethyl acetate추출물의 항균활성이 가장 뛰어났으며, 특히, 김치의 연부에 관여하는 Brettanomyces custersii, Klebsiella oxytoca, Pichia membranaefaciens에 대하여 강한 항균력을 보였고, 이 항균력은 미생물의 생육 pH가 7일때보다 pH 5일 때 높은 것으로 나타났다. 또한, 대조구로 사용한 0.5%와 1% sorbic acid보다도 더 높은 항균력을 보여 주었으며, 일반 식품 오염 미생물인 E. coli, B. subtilis, S. aureus 등에 대해서도 높은 항균력을 보여주어 광범위한 항균 spectrum을 나타냈다.

• Journal of Nutrition and Health
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• 제37권8호
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• pp.655-661
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• 2004

This study was performed to investigate the antimicrobial effect of the Pulsatilla koreana extracts against food-borne pathogens. First, the Pulsatilla koreana was extracted with methanol at room temperatures, and fractionation of the methanol extracts from Pulsatilla koreana was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of the Pulsatilla koreana extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Pulsatilla koreana showed the highest antimicrobial activity against Staphylococcus aureus, Salmonella enteritidis and Shigella dysenteriae. The Staphylococcus aureus and Shigella dysenteriae were inhibited by petroleum ether and chloroform extracts of Pulsatilla koreana as well as ethyl acetate extracts of Pulsatilla koreana. The synergistic effect has been found in combined extracts of Pulsatilla koreana and Portulaca oleracea as compared to each extracts alone. Finally, the growth inhibition curve was determined using ethyl acetate extracts of Pulsatilla koreana against Staphylococcus aureus and Shigella dysenteriae. The ethyl acetate extract of Pulsatilla koreana showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 2,000 ppm. The 2,000 ppm of ethyl acetate extract from Pulsatilla koreana retarded the growth of S. aureus more than 12 hours and Shigella dysenteriae up to 9 hours.

• Journal of Ginseng Research
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• 제8권1호
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• pp.22-31
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• 1984

Volatile flavor components of fresh ginseng (Panax ginseng C.A. Meyer.) were studied. Steam distillate of fresh ginseng was extracted with ethyl ether and the extract was separated into four fraction: neutral, phenolic, acidic and basis fractions. The ethyl ether concentrates and neutral fraction were analyzed by a combination of SE-54 fused silica capillary gas chromatography and mass spectrometry. Major flavor components of fresh ginseng were predominantly mono(n +2) and sesquiterpenes(n +3) in over two hundred constituents. Of these, 28 were newly identified in volatile flavor components of fresh ginseng by GC-MS.

• 한국식품영양과학회지
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• 제33권7호
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• pp.1106-1111
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• 2004

본 연구에서는 식중독 유발세균에 대한 항균활성이 우수한 천연 항균성 물질을 검색하기 위 해 예로부터 민간과 한방에서 널리 이용되어 온 산두근을 각종 유기용매로 추출하여 식중독 유발세균에 대한 항균활성을 조사해 보았다. 산두근을 methanol로 추출한 후, petroleum ether, chloroform, ethly acetate를 이용하여 실온에서 각각 용매별로 계통 분획하고, 열수추출물을 얻은 후,9종의 식중독 유발세균(Staphylococcus aureus, Bacillus cereus, Salmonella Enteritidis, Shigella flexneri, Escherichia coli, Salmonella Typhimurium, Shigella dysenteriae, Pseudomonas aruginosa, Shigella sonnei)에 대하여 항균효과를 조사하였다. 산두근 추출물의 농도별 항균 활성 검색에서는 산두근의 ethyl acetate추출물이 가장 큰 항균 효과를 보였으며 Staphylococcus aureus와 Shigella dysenteriae가 가장 민감하게 반응하는 균주였다. 산두근의 ethyl acetate 추출물과 백두옹의 ethyl acetate 추출물을 혼합하여 항균력을 측정 해 본 결과 두 추출물을 섞어 첨가했을 경우가 단독으로 사용했을 때보다 상승효과를 나타내었다. 또한 산두근의 ethyl acetate 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 Staphylococcus aureus 및 Shigella dysenteriae의 배양액에 산두근의 ethyl acetate추출물을 각각 4,000 ppm 농도로 첨가했을 시, Staphylococcus aureus의 생육이 24시간 이상까지 억제됨을 관찰할 수 있었고, Shigella dysenteriae의 생육도 48시간까지 지연시킬 수 있었다. 이상의 결과 산두근의 ethyl acetate 추출물은 식품보존제 등의 신소재 물질로 활용가능성이 높음을 알 수 있었다.

• KSBB Journal
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• 제23권3호
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• pp.219-225
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• 2008

본 연구는 국외토양으로부터 분리한 400여개의 군주에서 agar plug assay를 이용하여 식물병원균에 대한 항진균성물질을 생산하는 방선균 (I-8)을 분리하였다. 항진균 물질 생산의 최적 조건을 검토한 결과 공시균 I-8은 pH 8로 조정된 ISP No. 2 배지에서 항진균물질 생산성이 가장 우수한 것으로 입증되었다. 공시균 I-8의 식물병원균의 생육저해 기구를 규명하기 위한 기초 연구로서 공시균 I-8이 세포벽 구성성분인 cellulase, chitinase와 같은 가수분해효소를 생산하는 것을 확인하였다. I-8의 배양액은 ethyl acetate추출물에서 식물병원균 14개 균주에 항진균 활성을 나타내었고 ethyl ether추출액에서는 식물병원균 10개 균주에 항진균 활성을 나타내었다. I-8의 ethyl acetate추출물은 공시균인 13-16의 추출물과 혼합사용시 식물병원균인 Monosporascus cannonballus KACC 40940에 대한 항진균력의 상승효과를 확인할 수 있었다. I-8균주 배양체는 고체배지상에서 16개의 식물병원균에 대해 강한 생육저해 효과를 보여, 광범위한 항진균 활성을 나타내는 것을 확인하였다.

• 동의생리병리학회지
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• 제21권1호
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• pp.153-157
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• 2007

In order to evaluate the cancer preventive and estrogenic compounds in soybean and Cheongkukjang, MTT assay and in vitro test system for the evaluation of the estrogenic activity were applied. The fractions from the ethanol extract of soybean and Cheongkukjang were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, butanol, methanol and H$_2$O. Ethyl ether fractions of soybean and Cheongkukjang showed the highest cytotoxicity against U937 cell line in dose dependent manner, and ethyl ether fraction of Cheongkukjang showed two times higher cytotoxicity than that of soybean. Aqueous fraction of soybean and ethyl ether fraction of Cheongkukjang revealed the highest estrogenic activity and activity was higher in the fractions of Cheongkukjang than soybean. Mixture of Spirulina and Cheongkukjang showed synergistic activity. These observations concerning cancer preventive and estrogen effects of soybean and Cheongkukjang suggest that these materials possess useful ingredients for the prevention of cancer and/or postmenopausal disorder.

• Food Science and Biotechnology
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• 제15권6호
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• pp.889-895
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• 2006

Cytotoxic effects of extracts from Tremella fuciformis strain FB001 were evaluated on the DLD-1 human colon adenocarcinoma cell line and the content of polyphenolic compounds in the extracts were analyzed. Hexane, chloroform, and ethyl acetate subfractions (experimental setting I) exhibited cytotoxic effects on the human colon adenocarcinoma DLD-1 cell line with $IC_{50}$ values of 350, 400, and 450 ppm, respectively. When T. fuciformis was extracted sequentially with ether, ethyl acetate, chloroform, and ethanol (experimental setting II), the ether extract demonstrated potent cytotoxicity with an $IC_{50}$ value of 150 ppm, followed by ethyl acetate and chloroform fractions. If the first extraction solvent was chloroform instead of ether (experimental setting III), exposure of the cell line to chloroform, ethyl acetate, and ether extracts at 1,000 ppm led to cell death. High levels of phenolic compounds were estimated for all hydrophobic extracts, which exhibited cytotoxic effects. We propose that this useful information gives additional support to our understanding of the biology and utility of this particular mushroom.

• 한국식품과학회지
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• 제25권5호
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• pp.526-529
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• 1993

전통기호음료성분에 의하여 나타나는 기능특성을 조사하기 위한 연구의 일환으로 결명자의 메탄올 추출물을 유기용매에 의한 분획, silica gel column chromatography, thin layer chromatography에 의하여 여러가지 획분으로 분획하여 아질산염 소거작용을 조사한 결과, 아질산염 소거율은 ethyl acetate 획분>chloroform 획분>수용성 획분>ethyl ether 획분의 순으로 나타났으며, ethyl acetate 획분에서 분리한 compound D의 아질산염 소거효과는 매우 강력하여 ascorbic acid보다 10배 뛰어났다.

• Journal of Nutrition and Health
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• 제36권2호
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• pp.147-153
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• 2003

In this study, Artemisia capillaries, which has been used as a folk remedy, was investigated for its antimicrobial activity. First, the Aremisia capillaris was extracted with methanol at room temperature, and fractionation of the methanol extracts from Artemisia capillaris was carried out using petroleum ether, chloroform, and ethyl acetate. Second, the antimicrobial activity of the Artemisia capillaris extracts was determined using a paper disc method and minimum inhibitory concentration of ethyl acetate extracts from Artemisia capillaris against food-borne pathogens and food spoilage bacteria was measured. Finally, the growth inhibition curve was determined using ethyl acetate extracts of Artemisia capillaris against Staphylococcus aureus and Salmonella typhimurium. The ethyl acetate extract of Artemisia capillaris showed strong antimicrobial activity against S. typhimurium at a concentration of 1,000 ppm. The 3,000 ppm of ethyl acetate extract from Artemisia capillaris retarded the growth of S. aureus and S. typhimurium for up to 6 hours.

• Preventive Nutrition and Food Science
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• 제9권4호
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• pp.306-311
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• 2004

This study was performed to investigate the antimicrobial effects of Portulaca oleracea extracts against food-borne pathogens. First, the Portulaca oleracea was extracted with methanol at room temperature, and then further fractionated by using petroleum ether, chloroform, ethyl acetate and methanol, respectively. The antimicrobial activity of the Portulaca oleracea extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Portulaca oleracea showed the highest antimicrobial activity against Staphylococcus aureus and Shigella dysenteriae. There was also a synergistic effect of the combined extracts of Portulaca oleracea and Indigofera kirilowii as compared to each extract alone. Finally, the growth inhibition curve of ethyl acetate extracts of Portulaca oleracea against Staph­ylococcus aureus and Shigella dysenteriae was determined The ethyl acetate extract of Portulaca oleracea showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 4,000 ppm. The 4,000 ppm of ethyl acetate extract from Portulaca oleracea, retarded the growth of S. aureus by more than 24 hand Shigella dysenteriae up to 12 h at $37^{\circ}C$.