• Bulletin of the Korean Chemical Society
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• 제15권1호
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• pp.9-12
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• 1994

The gravimetric determination of scandium by di-(2-ethylhexyl)phosphate(DEHPA) as a precipitant and the mole ratio of Sc-DEHPA arecipitation obtained in ethanol medium have been investigated. Scandium can be determined by gravimetric method of precipitation of Sc-DEHPA and the mole ratio of Sc-DEHPA is found as 1 :2 in ethanol medium.

• 한국식품과학회지
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• 제29권4호
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• pp.752-757
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• 1997

Papaya 유액 추출물에서 protein을 정제 회수하기 위해 papaya 유액으로부터 추출한 추출액을 경제적이고도 처리가 비교적 간단한 ethanol 침전방법을 사용하였다. 실험계획법에 의하여 설정된 3수준의 부분요인 실험법에 의하여 실시된 각 처리구의 실험 결과를 다중 회귀분석 방법에 의해 다음과 같은 모델식 $Y=7.4554+1.2657X_{2}+1.0552X_{3}-1.7407X_{4}-0.01666X_{2}\;^{2}-0.1134X_{4}^{2}$을 산출하였으며 pH는 다중 회귀분석에 의해 제거되었다. 반응표면 분석법(RSM)을 이용한 결과 protein을 회수하기 위한 최적 조건으로는 protein 농도가 38.2 mg/ml, 40%의 ethanol 농도, 침전 온도는 $-8^{\circ}C$였다. 이러한 최적 조건에 의한 회수율의 실험치는 68.97%로 예측치인 77.28%에 근접한 결과였다. 또한 유의 수준을 검정하는 자유도와 F-value도 양호한 값(p<0.001)을 나타내어 종속변수와 독립변수와의 상관관계가 뛰어남을 알 수 있으며 따라서 다중 회귀분석에 얻어진 모델식의 각 변수들 사이에는 유의성이 높음을 알 수 있었다.

• Journal of Ginseng Research
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• 제19권3호
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• pp.244-248
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• 1995

Water-soluble browning pigments were artily purified from Korean red ginseng through the several procedures such as fractionation by n-butanol, precipitation by ethanol, dialysis and gel filtration. At least four kinds of water-soluble browning pigment were separated from each other, two kinds of low-molecular-weight and two kinds of high-molecular-weight pigments.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.139.2-139.2
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• 2003

The in vitro effects of extracted fractions of C. militaris on the secretion of cytokines in murine macrophage cell line, RAW 264.7 were studied. F1 (crude cordycepin containing adenosine), F2 (ethanol precipitation), F3 (ethanol soluble supernatant) and F4 (fraction of through SK-1B) significantly stimulated the production of cytokine and nitric oxide (NO) on murine macrophage cell line RAW264.7. We examined how the ethanol extract of C. militaris regulates production of interleukine 1-beta(IL-1$\beta$), tumor necrosis factor-alpha (TNF-$\alpha$), and NO in vitro. (omitted)

• 미생물학회지
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• 제9권4호
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• pp.175-178
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• 1971

Because of the cases of Japanese Encephalitis(J.E.) were reported every year in Korea. We, Dong-A Pharmaceutical Co., Ltd., produced J.E. virus vaccine, with lower price, since 1970 in order to prevent ourselves from being infected by the disease. And inoculated the J.E. virus vaccine for the children with a great success. We are going to report several questions which brought about in producing the J.E. virus vaccine by alcohol precipitation, protamine sulfate treatment method. The results obtained were as folows ; 1) In process treated with 40% alcohol, we used to ethanol made in Germany, but it was too expensive to use it. As the result which we had studied about it, we were satisfied with J.E. virus vaccine which produced with alcohol made in Korea, and then, we treated with accurate specific gravity of 40% ethanol for the precipitation of the virus. And also, we knew that it was the best method to be treated it for 3hrs, $13^{\circ}C$. 2) When we treated with protamine sulfate (0.025mg/ml), we acquired the highest potent titer, and suited into purpose for the nitrogen concentration. 3) The filtration of the purified J.E. virus vaccine, in case of millipore filter paper of large pore size was not suitable for the sterility. Therefore the pore size less than 0.8.$\mu$ (AA filter paper) in millipore filter paper was very suitable. But it seemed to be important subhects that the smaller was the pore size, the lower was the potent titer.

• 유기물자원화
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• 제26권1호
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• pp.47-53
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• 2018

유기성 폐기물 소화공정의 발효액에서 젖산(lactic acid)을 회수하기 위해 화학침전법을 평가하였다. 젖산(lactic acid)의 회수율을 높이기 위하여 화학침전제 종류와 교반속도 및 침전시간 등 반응조건이 회수율 향상에 미치는 영향을 살펴보았다. 화학침전제의 종류에 관계없이 주입양이 증가할수록 젖산(lactic acid) 회수율이 증가하는 경향을 보였으며, CaO가 $Ca(OH)_2$와 $CaCO_3$에 비하여 높은 회수율을 나타냈다. CaO를 사용한 반응조건 최적화 실험결과, 교반속도 180 rpm, 침전시간 24 h, ethanol 주입량 25%(v/v)에서 회수율이 48%로 가장 높게 나타났다. 본 연구에서는 발효액 내 젖산(lactic acid)의 농도만 고려하여 주입할 침전제의 양을 계산하였기 때문에 실제 적용을 위해서는 발효액의 유기산 종류 및 농도를 고려하여 침전제의 투입량을 결정해야할 것이다. 유기물의 정성적 분석(FEEM, SEC) 결과로 볼 때 침전공정은 유기산의 특성에 큰 영향을 미치지 않았다.

• 생약학회지
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• 제29권2호
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• pp.120-124
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• 1998

For the purpose of evaluating protective components against alcohol-induced toxicity, the active components enhancing alcohol metabolism was pursued from water soluble fraction by ethanol precipitation and DEAE-cellulose chromatographic technique. As a result, various high molecular weight fractions from Aloe vera and Aloe arborescens, on a single oral administration in rats were found to cause a significant decrease in the blood ethanol concentration as well as enhancement of liver cytosolic ADH and ALDH activities and among which, a strong acidic high molecular weight fraction was demonstrated to exhibit the most potent enhancing activity on ethanol metabolism.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.264-264
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• 2012

As an environment-friendly alternative energy resource, ethanol may be used to obtain hydrogen, a clean energy source. Thus, studies on catalytic reactions involving ethanol have been studied to understand the underlying principles in the reaction mechanism using various oxide-supported catalysts. Among them, Au-based catalysts have shown a superior activity in producing hydrogen gas. In the present study, Au/$TiO_2$ catalysts were prepared by deposition-precipitation method to understand their catalytic activities toward ethanol and acetaldehyde with increasing gold loading, especially at the very low Au loading regime. A commercially available $TiO_2$ (Degussa P-25) was employed and the Au loading was varied to 0, 0.1, 0.5, and 1.0 wt% respectively. The catalysts showed characteristic x-ray diffraction (XRD) features at $2{\theta}=78.5^{\circ}$ that could be assigned to the presence of gold nanoparticles. Its reactivity measurements were performed under a constant flow of ethanol and acetaldehyde at a flow rate of ${\sim}0.6{\mu}mol/sec$ and the substrate temperature was slowly raised at a rate of 0.2 K/sec. We observed that the overall reactivity of the catalysts increased with increasing Au loading along with selectivity favoring dehydrogenation to product hydrogen gas. In addition, we disclosed various reaction channels involving competitive reaction paths such as dehydrogenation, dehydration, and condensation. In addition, subsequent reactions of acetaldehyde obtained from dehydrogenation of ethanol, were found to occur and produce butene, crotonaldehyde, furan, and benzene. Based on the results, we proposed overall reaction pathways of such reaction channels.

• Applied Biological Chemistry
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• 제39권3호
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• pp.218-221
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• 1996

가공적성이 우수한 알코올처리 각시가자미껍질 젤라틴을 식품산업소재로 이용하기 위한 기초자료를 얻을 목적으로 가공시 많이 이용하거나 혼입될 우려가 있는 철이온, 식염, 당, 산 및 알코을 등에 의한 알코올처리 젤라틴의 물리적 특성의 변화를 무처리 젤라틴과 비교하여 검토하였다. 겔강도, 졸화온도, 겔화온도 및 점도와 같은 물리적 특성치는 에탄올처리의 유무에 관계없이 두 젤라틴 모두가 철이온 , 당 및 에탄올을 첨가한 경우 증가하였으나, 염화나트륨 및 유기산을 첨가한 경우는 감소하는 경향을 나타내었다. 첨가물에 의한 물리적 특성의 변화폭은 에탄올처리 젤라틴이 무처리 젤라틴에 비하여 식염, 당 및 에탄올을 가한 경우 차이가 없었으나 철이온 및 산을 가한 경우 적었다.

• 한국식품영양과학회지
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• 제17권2호
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• pp.136-143
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• 1988

발효단백질 중의 유리아미노산을 효과적으로 정량하기 위하여 정량방법(추출조건, 제단백(除蛋白) 및 발색법)에 따른 영향을 검토하였다. 낙농발효품의 수용성 유리아미노산은 $75^{\circ}C$, 40분, 대두발효품은 $40^{\circ}C$, 3시간에서 효과적으로 추출되었다. 여러 침전방법(95% ethanol 처리, $100^{\circ}C$, 3분 가열 및 1% picric acid phthaldialdehyde로 발색시켜 D.L.-lysine을 표준아미노산으로 삼아 정량, 계산하였을 때가 아미노산자동분석기에 의한 결과에 가장 접근하였으나 ethanol로 침전시킨 뒤 OPDA로 발색시킨 결과도 이와 유사하였다. TNBS(2,4,6-trinitrobenzensulfonic acid)에 의한 발색정량에는 ethanol 침전처리가 가장 효과적이었으나 종래의 동감법(銅監法)(copper salt method, Spies and Chamber, 1958.)에 의한 결과는 다른 비색정량 및 아미노산자동분석기에 의한 결과에 비하여 시료에 따른 과소의 차이가 심하였고 재현성이 결여되었다.