• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.82-83
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• 제23권3호
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• pp.326-332
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• 2010

Daidzein, a natural isoflavonoid phytoestrogen, structurally resembles estradiol (E2) and possesses estrogenic activity. This study was designed to test the hypothesis that daidzein may mimic the effects of E2 on ovine follicle development by regulation of the mRNA expression of bone morphogenetic protein receptor genes and thereby influence the reproductive system. Granulosa cells were cultured in serum-free McCoy's 5A medium with and without supplementation of daidzein. Results showed that daidzein (10-100 ng/ml) significantly increased the proliferation of ovine granulosa cells (p<0.05), but inhibited the growth of granulosa cells at a dose of 1,000 ng/ml (p<0.01). Daidzein inhibited progesterone production in a dose dependent manner; however, it did not affect estradiol production by granulosa cells. We also investigated the effects of daidzein on BMPRII, BMPRIB and ALK-5 mRNA expression in ovine granulosa cells by quantitative real-time PCR. Treatment of granulosa cells with daidzein increased significantly expression of these genes at 10-100 ng/ml. Thus, these data suggested that a low concentration of daidzein (10-100 ng/ml) had a direct stimulatory effect on ovine granulosa cells while a high concentration was toxic.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.112-112
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• 2001

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an environmental toxin that activates the aryl hydrocarbon receptor (AhR) and disrupts multiple endocrine signaling pathways by enhancing ligand metabolism, altering hormone synthesis, down regulating receptor levels, and interfering with gene transcription. And TCDD-mediated gene transactivation via the AhR has been shown to be dependent upon estrogen receptor (ER) expression in human breast cancer cells. In the present study, we have examined the effect of natural estrogen, phytoestrognes and environmental estrogens on the regulation of CYP1A1 gene expression in MCF-7 human breast cancer cell line. that ER and AhR are co-expressed. pCYP1A1 -luc reporter gene was transiently transfected into MCF-7 cells. These cells were treated with various chemicals and then luciferase assay was carried out. 17be1a-estradiol significantly inhibited TCDD stimulated luciferase activity dose dependently and this inhibition was partially recovered by concomitant treatment of tamoxifen. 17beta-estradiol metabolites, 2-hydroxyestradiol and 16alpha-estriol resulted in less potent inhibitory effect than estradiol and synthetic estrogen, diethylstilbestrol (DES) showed no effect on CYP1A1 gene expression. This study demonstrated that estrogen down-regulated TCDD stimulated CYP1A1 expression via ER mediation. And we have found out that several flavonoids such as genistein, kaempferol, daidzein, naringenin, and alkylphenols such as nonylphenol, 4-octylphenol and resveratrol also inhibited TCDD induced CYP1A1 expression like estrogen.

• 약학회지
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• 제45권6호
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• pp.694-700
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• 2001

Synthetic pyrethroids are analysis of a natural chemical moiety, pyrethrin derived from the pyrethrum plant Chrysanthemum. The natural pyrethrin structure has been modified to be highly lipophilic and photostable, creating an effective pesticide and resulting in an increased presence in the environment. Worldwide, they are commonly used insecticides against ticks, mites, mosquitoes, and as treatment for human head lice and scabies. Therefore, human exposure to their compounds in extensive. Several studies on the effects of pyrethroids on thyroid hormone regulation, estrogen and androgen function have been reported and yet little has been done try assess their potential hormonal activities. Among humans, a pyrethroid compound was suggested to be the causal agent for gynecomastia in a group of Haitian men. The reports suggest that some pyrethroid compounds are capable of disrupting endocrine function. Therefore, we examined estrogenic/antiestrogenic potential of three pyrethroid insecticides, that is permethrin, allethrin and fenvalerate in human breast cancer cell and action mechanism mediated by the estrogen receptor. Fenvalerate showed weak estrogenic activity but aallethrin and permethrin showed no effect. In combination with high levels (10$^{-10}$ M, 10$^{-11}$ M) of 17$\beta$-estradiol and three synthetic pyrethroids inhibited cert proliferations in MCF7-BUS cell by 17$\beta$-estradiol. Whereas, fenvalerate increased cell proliferative activity at lower level of estradiol (10$^{-12}$ M, 10$^{-13}$ M). The relative affinities to the estrogen receptor were observed by allethrin and permethrin treatment, but not by fenvalerate. These results indicated that some of pyrethroid insecticides may modulate estrogen functions in human breast cancer cell. The action mechanisms of estrogen receptor mediated antiestrogenicity by allethrin and permethrin were postulated.

• 한국해양바이오학회지
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• 제12권2호
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• pp.75-80
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• 2020

In the present study, the estrogenic activity and anti-osteoclastogenic activity of the Myelophycus simplex extract were evaluated using T47D-Kbluc cells and bone marrow-derived macrophages (BMMs). As a result of the measurement of the estrogenic activity in the T47D-Kbluc cell line, the Myelophycus simplex extract showed increased estrogenic activity in a dose-dependent manner in association with its concentration. To confirm the regulatory effect of the Myelophycus simplex extract on the estrogen-responsive gene, the Myelophycus simplex extract showed a similar tendency to estradiol: the expression of estrogen receptor 1 (ESR1) was significantly decreased while the expression of estrogen receptor 2 (ESR2) was increased. Furthermore, the Myelophycus simplex extract exhibited an inhibitory effect on osteoclast differentiation. In conclusion, these Myelophycus simplex extracts might be regarded as candidates for further studies or the development of functional food products or medicine to prevent or avoid postmenopausal symptoms for women.

• 대한핵의학회지
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• 제34권5호
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• pp.403-409
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• 2000

목적: 에스트로겐 수용체의 불균질 분포와 시간 변화에 따른 수용체 상태의 변화 때문에 조직검사를 통한 에스트로겐 수용체의 생화학적 측정은 유방암을 진단하기에 충분하지 못한 단점이 있다. 이런 단점을 개선하고 좀더 나은 유방암의 진단을 위하여 E-$[^{123}I]$IVE에 대하여 연구하였다. 본 연구에서는 에스트로겐 수용체 양성 유방암 환자의 영상을 얻기에 적합한 방사성 추적자를 개발 하였다. 대상 및 방법: 여러 가지 에스트라디올 유도체 중에서 $17{\alpha}$-ethynyl estradiol로부터 $17{\alpha}-[^{123}I]$iodovinyl estradiol ($[^{123}I]$IVE)을 합성하였다. E-$[^{123}I]$IVE는 peracetic acid와 $[^{123}I]$NaI를 이용하여 합성하였고, Z-$[^{123}I]$IVE는 chloramine-T/HCl과 $[^{123}I]$NaI로 합성하였다. 표지수율은 TLC-scanner를 이용해 측정하였으며, 방사 화학적 순도는 HPLC로 측정하였다. E-$[^{123}I]$IVE의 체내 동태 연구는 immature female Fisher rate를 사용해 60분, 120분 그리고 300분에 측정하였다. 결과: E-$[^{123}I]$IVE와 Z-$[^{123}I]$IVE의 표지수율은 각각 92%와 94%이었고, 정제 후 방사 화학적 순도는 98% 이상이었다. E-$[^{123}I]$IVE의 최고섭취는 uterus에서 주사 120분 후에 관찰되었다(3.11%ID/g). 결론: 이상의 결과를 종합해 볼 때 E-$[^{123}I]$IVE는 유방암환자의 에스트로겐 수용체의 존재를 평가하는 진단시약으로서의 사용 가능성을 확인하였다.

• 운동과학
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• 제21권2호
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• pp.243-254
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• 2012

본 연구는 폐경에 따른 에스트로겐 대사의 부정적인 영향의 방지 및 처치를 위한 효율적인 대체요법으로서 운동과 콩 단백질 추출물 섭취 프로그램의 활용가능성을 제시하고자 한다. 이에 난소 절제하여 폐경과 동일한 상태를 유도한 흰쥐를 대상으로 8주 동안 트레드밀을 이용한 지구성 운동과 콩 단백질 추출물 섭취의 복합적인 처치가 신체구성 및 골밀도, 혈중 에스트라디올농도, 간 조직에서의 에스트로겐 수용체의 유전자 발현양상, 혈중 글루코스, 중성지방, 인슐린 및 CRP 농도 등의 변화에 미치는 영향을 분석하였다. 난소절제그룹은 체중 및 체지방률이 증가하고, 혈중 에스트라디올농도 및 간 조직에서의 에스트로겐 수용체의 유전자 발현이 감소하였으며, 혈중 중성지방농도는 증가하였다. 8주간의 운동 및 콩 단백질 섭취에 의한 처치 후 에스트라디올농도, 에스트로겐 수용체 및 혈중 변인 등에 긍정적인 효과를 나타냈으나 골밀도에는 영향을 미치지 못한 것으로 나타났다. 따라서 난소절제 흰쥐의 8주간 운동 및 콩 단백질 추출물 처치가 에스트로겐 및 지방대사에 긍정적인 영향을 미칠 수 있는 가능성은 확인 되었으나, 운동강도 및 시간, 콩 단백질의 섭취량을 포함한 처치기간 등에 대한 계속적인 검토가 요구된다고 생각된다.

• 대한수의학회지
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• 제37권2호
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• pp.311-319
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• 1997

The mechanisms of $estradiol-17{\beta}$ regulating growth of both normal and neoplastic cells are not clear until now. In studies using various estrogen-dependent breast cell lines, it is recently known that estrogen controls the cell growth by regulating the expression of growth factors and/or their receptors. In the present study, we investigated the effects of $estradiol-17{\beta}$on cell growth and IGF-I binding sites using primary cultured renal proximal tubule cells. We have obtained results as follows : $Estradiol-17{\beta}(10^{-9})$ has stimulatory effects in cell growth. Cotreatment of $estradiol-17{\beta}(10^{-9}M)$ and $IGF-I(5{\times}10^{-8}M)$ significantly increased the growth of primary rabbit renal proximal tubule cells compared to that of $estradiol-17{\beta}$ or IGF-I alone treated cells. In binding studies, we found that the binding of $^{125}IGF-I$ on cell membranes was incubation time- and temperature-dependent. Incubation at $37^{\circ}C$ results in higher binding of $^{125}IGF-I$ than that of $23^{\circ}C$ or $4^{\circ}C$. Maximum binding was observed at $37^{\circ}C$ between 30 and 60 minutes. The binding of $^{125}IGF-I$ to both control and $estradiol-17{\beta}-treated$ cells was inhibited by unlabelled $IGF-I(10^{-8}{\sim}10^{-12}M)$ in a concentration-dependent manner. However, EGF did not compete for $^{125}IGF-I$ binding at $10^{-8}{\sim}10^{-12}M$. IGF-I binding to the membranes from both control and $estradiol-17{\beta}-treated$ cells was also analyzed. We found that $estradiol-17{\beta}-treated$ cells exhibited higher binding activity for IGF-I. When $estradiol-17{\beta}$ or tamoxifen alone, or $estradiol-17{\beta}$ and tamoxifen cotreated cells were compared, the binding ratio of $^{125}I-IGF-I$ of $estradiol-17{\beta}-treated$ cell was significantly increased but was similar to control in both $estradiol-17{\beta}$ and tamoxifen cotreated cell. These results suggest that $estradiol-17{\beta}$ in part controls cell proliferation by regulating the expression of IGF-I receptors in primary rabbit renal proximal tubule cells.

• 한국임상수의학회지
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• 제18권4호
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• pp.448-451
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• 2001

Alopecia was the main complaint in a 4 year old female Cocker spaniel dog. bilateral alopecia has been presented on skin lesion for 10 months after ovariohysterectomy about one year ago. Hyperestrogenism was diagnosed by the blood estradiol level test. Left ovarian cyst was resected in the intact ovarian position by laparotomy. Total size of the cyst was about 3 cm and it was the shape of follicular cyst. Blood estradiol level is usually higher than normal in ovarian cyst. But even though estradiol level is in normal range, dermatitis could be induced because the number of estrogen receptor of the skin would be increased or the duration of estrogen secretion would be prolonged. Because the hyperestrogenism in this case is iatrogenic, exact and proper ovariohysterectomy should be made not to induce the complications.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3681-3685
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• 2012

Background: In breast cancer, estrogen receptors have been demonstrated to interact with transcription factors to regulate target gene expression. However, high-throughput identification of the transcription regulation relationship between transcription factors and their target genes in response to estradiol is still in its infancy. Purpose: Thus, the objective of our study was to interpret the transcription regulation network of MCF7 breast cancer cells exposed to estradiol. Methods: In this work, GSE11352 microarray data were used to identify differentially expressed genes (DEGs). Results: Our results showed that the MYB (v-myb myeloblastosis viral oncogene homolog [avian]), PGR (progesterone receptor), and MYC (v-myc myelocytomatosis viral oncogene homolog [avian]) were hub nodes in our transcriptome network, which may interact with ER and, in turn, regulate target gene expression. MYB can up-regulate MCM3 (minichromosome maintenance 3) and MCM7 expression; PGR can suppress BCL2 (B-cell lymphoma 2) expression; MYC can inhibit TGFB2 (transforming growth factor, beta 2) expression. These genes are associated with breast cancer progression via cell cycling and the $TGF{\beta}$ signaling pathway. Conclusion: Analysis of transcriptional regulation may provide a better understanding of molecular mechanisms and clues to potential therapeutic targets in the treatment of breast cancer.