• Food Science and Biotechnology
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• v.16 no.4
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• pp.531-536
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• 2007

The aerial part of Artemisia iwayomogi Kitamura has traditionally been used for inflammation, infectious disease, cancer, pyretic, diuretic, liver protective effect, and choleretic purposes in Korea. We investigated that the essential oil induces apoptosis in KB cell as evidenced by Hoechst-33258 dye staining, flow cytometry (cell cycles), and DNA fragmentation for nuclear condensation and Western blotting for activation of caspases-3, -8, -9, Bax, Bcl-2, cytochrome c, and poly (ADP-ribose) polymerase (PARP) cleavage. In the present study, we found that the essential oil could induce apoptosis in KB cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. The efficacious induction of apoptosis was observed as a dose-dependent. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. The essential oil also caused the loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytosol. These findings indicate that mitochondrial pathways might be involved in the essential oil-induced apoptosis and enhance our understanding of the anticancer function of the essential oil in herbal medicine.

• Journal of Korea Foresty Energy
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• v.21 no.1
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• pp.7-15
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• 2002

The essential oil free needles, which were left after distillation of essential oil from various coniferous needles, were fermented with food waste organics in order to use as compost and roughage. Microorganisms for the fermentation were selected from domestic sources such as swine compost, bark compost, and kimchi, etc, and consisted of aerothermophilic bacteria and actinomycetes. The weight reduction ratio of food waste organics treated with the microorganisms was 90% after 30 days treatment, and the fermentation temperature was kept at approximately $45^{\circ}C$. The compost process was really slow due to chemical compounds derived from needles, and it finally took 60 days for complete compost. When 10% of needle compost was mixed with soil for radish growth, the growth indicators such as leaf length and root weight were increased compared with control, while root weight, root width, and root length were inhibited on the addition of 20% needle compost. The nutrient value and digestibility ratio of various essential oil free needles as roughage for ruminant animals were evaluated. The ratio of crude protein for essential oil free needles from Korean pine(Pinus koraiensis) was 10.02%, which was higher than those of rice straw(5.48%) and corn(9.00%). The digestibility ratios of essential oil free needles from Sawara cypress(Chamaecwaris pislfera), Korean pine(Pinus koraiensis), and Japanese Red Pine(Pinus densiflora) was 53%, 34%, 34%, respectively, indicating that those essential oil free needles were considered as excellent roughage.

• Natural Product Sciences
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• v.17 no.4
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• pp.296-302
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• 2011

We determined the inhibitory activity of the essential oil fraction obtained by steam distillation from the fresh and dried leaves of Perilla frutescens var. acuta against some pathogenic Salmonella and Vibrio spp. The activities of compounds isolated from the essential oils, apiol and myristicin, were also tested and the results were compared with those of the essential oil fraction. The Perilla essential oil fraction and its main components showed significant inhibition against antibiotic-susceptive and antibiotic-resistant strains of the tested Salmonella and Vibrio strains. Synergistic or additive effects were identified by combing the oils with ampicillin by checkerboard-titer tests. We conclude that essential oils from P. frutescens can be useful in the treatment of Salmonella and Vibrio infections and as safe additives to food materials for the prevention of contamination of food by these bacteria. This is especially important because of the rapid increase in antibiotic-resistant strains, which could cause severe symptoms in humans.

• Preventive Nutrition and Food Science
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• v.9 no.1
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• pp.1-6
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• 2004

Fresh ground beef was mixed with ascorbic acid, basil essential oil, majoram essential oil, or each essential oil combined with ascorbic acid and stored at 1 $\pm$ 1$^{\circ}C$ for 7 days. Color, lipid oxidation (TBARS formation), aerobic bacterial counts and pH were determined. Basil and majoram essential oils were effective in inhibiting color deterioration, lipid oxidation and bacterial growth. The combined addition of basil and ascorbic acid showed the highest protection against color fading, followed by majoram + ascorbic acid, and ascorbic acid alone. Basil and majoram essential oils were most effective in delaying TBARS formation (p < ().01). Ascorbic acid did not exert an antioxidative effect and even exhibited a pro-oxidant effect. The pH values of all samples increased slightly, but no significant differences were observed, either among treatments or throughout the storage time (p > 0.05).

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.156-165
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• 2017

This study investigated the chemical composition of Petasites japonicus (S. et Z.) Maxim essential oil. During the period 2011~2013, P. japonicus (S. et Z.) Maxim plant was investigated for composition of the essential oil. Chemical composition and characteristic compounds of the essential oils from the aerial parts of the plant according to the crop year studied. The essential oils consisted of sesquiterpene compounds, which were the most abundant components. Samples collected in 2011 were found to be richer in oxygenated sesquiterpenes, while samples collected in 2012 and 2013 were richer in diterpene alcohols and sesquiterpene hydrocarbons, respectively. Ninety-two compounds were identified in the P. japonicus (S. et Z.) Maxim essential oil of 2011, and caryophyllene oxide (20.49%), ${\beta}$-caryophyllene (10.28%), ${\beta}$-bisabolene (6.80%), and alloaromadendrene (6.50%) were the major compounds. Seventy-four compounds were identified in the plant essential oil of 2012, and phytol (17.22%), ${\alpha}$-farnesene (15.31%), ${\alpha}$-caryophyllene (9.93%), and ${\beta}$-caryophyllene (6.12%) were the major compounds. Ninety-two compounds were identified in the plant essential oil of 2013, and ${\alpha}$-farnesene (22.42%), ${\alpha}$-caryophyllene (21.49%), pentadecane (15.35%), and germacrene (5.70%) were the major compounds. The content of most of the chemical constituents varied significantly with different harvesting time. The content of ${\alpha}$-caryophyllene and caryophyllene oxide was increased significantly from 2011 to 2013. The content of ${\alpha}$-caryophyllene and isocaryophyllene was decreased significantly from 2011 to 2013.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.607-613
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• 2010

The influence of dietary supplementation with blended essential oil on growth performance, nutrient digestibility, blood profiles and fecal characteristics was evaluated in 125 crossed ((Duroc ${\times}$Yorkshire) ${\times}$Landrace) pigs (6.21${\pm}$0.20 kg initial body weight and 21 d average age). The pigs were allotted to the following treatments: i) NC (antibiotic free diet), ii) PC (NC diet+44 ppm tylosin), iii) T1 (NC diet+0.1% essential oil), iv) T2 (NC diet+0.1% essential oil (with 0.3% Benzoic acid)) and v) T3 (NC+22 ppm tylosin and 0.05% essential oil). Average daily gain (ADG) was improved in the T2 group on d 14 (p<0.05). In addition, nutrient digestibility was partially affected (both positively and negatively) by the treatments. Furthermore, the immune system was stimulated and the fecal pH and fecal noxious gases were improved in pigs that received the diets supplemented with essential oil (p<0.05). The appearance and score of diarrhea also tended to be lower in pigs that were subjected to the essential oil treatments. Collectively, the results of this study indicate that supplementation of the diet with blended essential oils could replace treatment with antibiotics to improve growth performance and fecal characteristics.

• Korean Journal of Pharmacognosy
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• v.47 no.2
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• pp.192-196
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• 2016

The herb of Agastache rugosa (Lamiaceae) called Korean mint as a spice or Agastache Herba as a crude drug is known to contain highly fragrant volatile substances. This research aimed to establish the quantitative gas chromatography (GC) method on the essential oil of A. rugosa using the three standard compounds, estragole, methyleugenol, pulegone, and to find whether the essential oil has anti-Alzheimer's activity. The GC quantification method was established by determining the linearity of calibration curve ($R^2$), linear range, and both limit-of-detection (LOD) and limit-of-quantification (LOQ). The $IC_{50}$ of the essential oil on the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were determined to be $69.06{\pm}0.26$ and $76.71{\pm}0.58{\mu}g/ml$, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.23 no.1
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• pp.65-70
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• 2001

In order to compare the extraction patterns of main components from the raw material between the extraction methods, the aerial parts (dried stem, leaves, and flowers) of Agastache rugosa were extracted by SDE simultaneous steam distillation & extraction) and SFE (supercritical fluid extraction). Volatile components of essential oil and extract were identified by GC and GC-MSD. The contents of essential oil extracted by SDE were 0.49% in aerial part of Agastache rugosa on dry basis. Major components were methyl chavicol(27.2%), isomenthone(24.6%), hexadecanoic acid(13.0%). menthone (5.5%) among 32 kinds of components confirmed in essential oil. On the other hand, the contents of SFE extracts revealed 3.21% on dry basis, 6 times higher than those of SDE. Major components were isomenthone(15.3%), hexadecanoic acid(13.7%), methyl chavicol(12.6%), benzoic acid(3.8%) among 33 kinds of components identified in extract.

• Fashion & Textile Research Journal
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• v.9 no.5
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• pp.569-572
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• 2007

The effectiveness of Palmarosa, Neroli and Jasmin blending oil on dry skin of rat induced by kitchen detergent are investigated. The experimental groups were divided the control group, group treated with surfactant, group treated with Palmarosa and Neroli, and group with Palmarosa, Neroli and Jasmin. Observation of epidermis and the alteration of mast cell were performed with photomicroscope. According to the epidermis morphological changes analysis, the A3 group treated with Palmarosa, Neroli and Jasmin blending oil was appeared the most similar with the control group, and the A2 group applied with Palmarosa and Neroli blending oil was sequently displayed similar characteristics. The collagen layer's breakaway resulting from Palmarosa essential oil, the collagen layer's restoration resulting from Neroli essential oil, the collagen layer's retention hyperkeratosis resulting from Jasmin essential oil were observed in the structure of the epidermal layer. In photomicrosope observation of mast cell to examine the inflammatory reactions, the increase in size and number of mast cell were showed in A1 group treated with surfactant compared to the control group. The number of mast cells definitely decreased in groups which were treated with Palmarosa, Neroli and Jasmin blending oil.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.125-128
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• 2016

The chemical compositions of the essential oil extracted from Albizziae julibrissin barks were analyzed by Gas chromatography-Mass spectrometry spectrometry. Fourteen components were identified, representing 89.23 % of the total oil composition. The analysis of the essential oil revealed that the essential oil contains 14 compounds, accounting for 89.23 % of the total oil. Hexanoic acid was the principal component (41.43 %) of the essential oil, followed by 4,4,6-trimethyl-cyclohex-2-en-1-ol (11.16 %), palmitic acid (9.00 %), 2-pentylfuran (5.66 %), 2-butyl-2-octenal (4.12 %), linoleic acid (3.10%), amyl hexanoate (3.01%), (E,E)-2,4-decadienal (2.49 %), 2-hexylthiophene (2.47 %), caprylic acid (2.13 %), ${\delta}-undecalactone$ (1.52 %), heptanoic acid (1.27 %), 3,5-octadien-2-ol (0.99 %), and 2-octenal (0.88 %). The acaricidal activity of the A. julibrissin oil was tested against Dermatophagoides farina, D. pteronyssinus and Tyrophagus putrescentiae by the fumigant bioassay. Based on the $LD_{50}$ values, the essential oil exhibited strong acaricidal activities against D. farinae ($LD_{50}$, $4.88{\mu}g/cm^3$), D. pteronyssinus ($2.44{\mu}g/cm^3$), and T. putrescentiae ($1.22{\mu}g/cm^3$). These results indicate that A. julibrissin oil could be a source of acaricidal agents for mite control.