• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1714-1727
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• 2015

This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm's initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.

• Journal of Korean Ophthalmic Optics Society
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• v.6 no.2
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• pp.121-127
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• 2001

While SVD and Gaussian elimination method were applied to the additive damped least squares(DLS), the convergence and the stability of the optimization process were examined in a triplet-type camera lens-system where the condition number is well conditioned. DLS with SVD method generated a suitable merit function but this merit function may be trapped in a local minimum by the nonlinearity of error function. Therefore, the least camera lens-system was further designed by the global optimization method is grid method, and this method is adopted to get merit function that convergent to global minimum without local minimum trapping.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.547-552
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• 2003

In mold machining, there are many concave machining regions where chatter and tool deflection occur since MRR (material removal rate) increases as curvature increases even though cutting speed and depth of cut are constant. Boolean operation between stock and tool model is widely used to compute MRR in NC milling simulation. In finish cutting, the side step is reduced to about 0.3mm and tool path length is sometimes over 300m. so Boolean operation takes long computation time and includes much error if the resolution of stock and tool model is larger than the side step. In this paper, curvature of CL(cutter location) surface and side step of tool path is used to compute the feedrate for constant MRR machining. The data structure of CL surface is Z-map generated from NC tool path. The algorithm to get local curvature from discrete data was developed and applied to compute local curvature of CL surface. The side step of tool path was computed by point density map which includes cutter location point density at each grid element. The feedrate computed from curvature and side step is inserted to new tool path to regulate MRR. The resultants wire applied to feedrate optimization system which generates new tool path with feedrate from NC codes for finish cutting. The system was applied to speaker mold machining. The finishing time was reduced to 12.6%. tool wear was reduced from 2mm to 1.1mm and chatter marks and over cut on corner were removed.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.11
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• pp.1179-1185
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• 2013

In this study, the design optimization of the automotive side member is performed to maximize the crash energy absorption efficiency per unit weight. Design parameters which seriously influence on the frontal crash performance are selected through the sensitivity analysis using the Plackett-Burman design method. And also the design variables, which are determined from the sensitivity analysis, are optimized by two methods. One is conventional approximate optimization method which uses the statistical design of experiments (DOE) and response surface method (RSM). The other is a methodology derived from previous work by the authors, which is called sequential design of experiments (SDOE), to reduce a trial and error procedure and to find an appropriate condition for using micro-genetic algorithm. The proposed optimization technique shows that the automotive side member structure can be designed considering the frontal crash performance.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.12 no.6
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• pp.461-468
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• 2002

Computer simulation is essential to design the suspension elements of railway vehicle. By computer simulation, engineers can assess the feasibility of the given design factors and change them to get a better design. But if one wishes to perform complex analysis on the simulation, such as railway vehicle dynamic, the computational time can become overwhelming. Therefore, many researchers have used a surrogate model that has a regression model performed on a data sampling of the simulation. In general, metamodels(surrogate model) take the form y($\chi$)＝f($\chi$)＋$\varepsilon$, where y($\chi$) is the true output, f($\chi$) is the metamodel output, and is the error. In this paper, a second order polynomial equation is used as the RSM(response surface model) for high speed train that have twenty-nine design variables and forty-six responses. After the RSM is constructed, multi-objective optimal solutions are achieved by using a nonlinear programming method called VMM(variable matric method) This paper shows that the RSM is a very efficient model to solve the complex optimization problem.

• Structural Engineering and Mechanics
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• v.46 no.5
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• pp.735-753
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• 2013

The wind load is always the dominant load of cooling tower due to its large size, complex geometry and thin-wall structure. At present, when computing the wind-induced response of the large-scale cooling tower, the wind pressure distribution is obtained based on code regulations, wind tunnel test or computational fluid dynamic (CFD) analysis, and then is imposed on the tower structure. However, such method fails to consider the change of the wind load with the deformation of cooling tower, which may result in error of the wind load. In this paper, the analysis of the large cooling tower based on the iterative method for wind pressure is studied, in which the advantages of CFD and finite element method (FEM) are combined in order to improve the accuracy. The comparative study of the results obtained from the code regulations and iterative method is conducted. The results show that with the increase of the mean wind speed, the difference between the methods becomes bigger. On the other hand, based on the design of experiment (DOE), an approximate model is built for the optimal design of the large-scale cooling tower by a two-level optimization strategy, which makes use of code-based design method and the proposed iterative method. The results of the numerical example demonstrate the feasibility and efficiency of the proposed method.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.5
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• pp.1286-1302
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• 2012

In this paper, the resource allocation problem with proportional fairness rate in cognitive OFDM-based wireless network is studied. It aims to maximize the total system throughput subject to constraints that include total transmit power for secondary users, maximum tolerable interferences of primary users, bit error rate, and proportional fairness rate among secondary users. It is a nonlinear optimization problem, for which obtaining the optimal solution is known to be NP-hard. An efficient bio-inspired suboptimal algorithm called immune clonal optimization is proposed to solve the resource allocation problem in two steps. That is, subcarriers are firstly allocated to secondary users assuming equal power assignment and then the power allocation is performed with an improved immune clonal algorithm. Suitable immune operators such as matrix encoding and adaptive mutation are designed for resource allocation problem. Simulation results show that the proposed algorithm achieves near-optimal throughput and more satisfying proportional fairness rate among secondary users with lower computational complexity.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.1A
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• pp.10-17
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• 2000

In this paper, we evaluate the correlation properties and the optimization of PN sequence phase for multi-media DS/CDMA system with variable spreading gain (VSG) scheme. In multi-media multi-rate DS/CDMA systems, the optimization of PN sequence phase is not a tractable problem, since the sequences should be optimized against both sequences of the same length and other sequences with different length. Hence, we verify the correlation properties of PN sequence phase in multi-rate system environment and furthermore, we propose the new phase criterion, MIN-AIP (minimum-average interference parameter), to minimize the bit error rate (BER). As the results of performance evaluations, it is shown that the performance of MIN-AIP criteria gives the best results.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.9
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• pp.1423-1431
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• 2001

Based on the exponential intervening variable, a new two-point approximation method is presented. This introduces the shifting level into each exponential intervening variable to avoid the lack of def inition of the conventional exponential intervening variables due to zero-or negative-valued design variables. Then a new quadratic approximation whose Hessian matrix has only diagonal elements of different values is proposed in terms of these intervening variables. These diagonal elements are determined in a closed form that corrects the typical error in the approximate gradient of the TANA series due to the lack of definition of exponential type intervening variables and their incomplete second-order terms. Also, a correction coefficient is multiplied to the pre-determined quadratic term to match the value of approximate function with that of the previous point. Finally, in order to show the numerical performance of the proposed method, a sequential approximate optimizer is developed and applied to solve six typical design problems. These optimization results are compared with those of TANA-3. These comparisons show that the proposed method gives more efficient and reliable results than TANA-3.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.12 no.2
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• pp.81-90
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• 2013

A study on the flow-structure characteristics of marine 3/2WAY pneumatic valve is essential for optimizing the performance of ship engines. It is important that the valve has desirable safety factor and reduced weight from safety and economic point of view. In this paper, flow-structure characteristics of pneumatic valve is obtained by being optimized based on the proper design criteria. The air with the pressure of 30 bar is the working fluid which is made to fill in the tack in short time. This time is defined as the filling time. On optimum design by considering the flow-structure characteristics, the approach is based on (1) the mathematical formulation of design decisions using the compromise decision-making method, and (2) the approximation technique of response surfaces. The methodology is demonstrated as the multi-objective optimization tool to improve the performance of marine 3/2WAY pneumatic valve.