• The Journal of Internal Korean Medicine
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• v.30 no.4
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• pp.845-857
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• 2009

Background and Objectives : Korean mistletoe lectin (Viscum album coloratum agglutinin, VCA) and bee venom (BV) have been reported to induce apoptosis in various cancer cell lines in vitro and to show antitumor activity against a variety of tumors in animal models. However, the comparative effect of VCA and BV on the anti-cancer effect and mechanisms of action has not been determined. In this study, the effect in a human hepatocellular carcinoma cell line, Hep G2 cells, was examined. Methods : Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay in litro. The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action were examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. The involvement of kinase was examined in VCA or BV-induced apoptosis by using kinase inhibitors. Results : VCA and BV killed Hep G2 cells in a time and dose-dependent manner. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including MAPK/ERK, p38 MAPK and JNK. BV also activated PARP-1, MAPK/ERK. and p38 MAPK but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not by BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli. VCA-induced apoptosis was partially inhibited by in the presence of JNK and p38 inhibitor, but BV only by p38 inhibitor. Conclusions : VCA-induced apoptosis is dependent on the activation of p38 and JNK. while BV-induced apoptosis is mediated by p38 activation in Hep G2 cells.

• Development and Reproduction
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• v.13 no.2
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• pp.89-95
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• 2009

p63 has been demonstrated to localize in stem cells and precursor cells of various epithelial tissues previously, but the localization of p63 throughout tooth formation, particularly during the enamel and root formation stages, remains to be adequately characterized. Therefore, in this study, we have demonstrated, via immunohistochemical methods, that p63 is ubiquitously expressed in the dental epithelium during tooth development. p63 was detected in the basal and suprabasal layers of the epithelia, including the skin, hair follicles, oral mucosa, and submandibular ducts. However, in the tooth region, all cells of the dental lamina, enamel organ, Hertwig's epithelial root sheath (HERS), and epithelial cell rests of Malassez (ERM) evidenced immunoreactivity for p63. These results indicate that p63 may perform different roles, other than stem cell maintenance, in tooth development.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.3
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• pp.295-300
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• 2010

The international convention on standards of training, certification and watch-keeping for seafarers, 1978(1978 STCW convention) has been established as a minimum standard and competence of seafarers, and contributed to reduce marine accidents caused by human errors. The convention has been partly amended several times according to environmental change of ship's operation and there were comprehensive revisions in so called 1995 amended STCW convention. In these years, ships are in tendency of increasing size and speed, and also adopted high technologies. These kinds of changes brought STW sub-committee up for discussions to modify the convention to cope with new environments from the year of 2006. As the result, major amendaments to STCW convention have been adopted at a diplomatic conference in Manila, Philippine in June 2010, thereby new global standards will be in force to train and certify seafarers to operate technologically advanced ships before long. This paper is to introduce major amendaments such as ship's security, ECDIS and leadership etc. in this Manila amendments and to clarify some articles to be adopted in programs for education and training of seafarers in Korea.

• Journal of the Society of Disaster Information
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• v.3 no.1
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• pp.103-116
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• 2007

Der Begriff des Todes $h{\ddot{a}}ngt$ die einzelne Wertvorstellung ab, so dass die Unanschaulichkeit des Todes besteht und noch zu $kl{\ddot{a}}ren$ ist. Im koreanischen Recht, findet sich keine Legaldefinition des Todes. In der juristischen Literatur herrscht der Herztod, nach dem $f{\ddot{u}}r$ den Todeszeitpunkt der irreversible und $vollst{\ddot{a}}ndige$ Stillstand der $Herzt\ddot{a}tigkeit$ gehalten wird, $herk{\ddot{o}}mmlicherweise$ bis jetzt als Todesdefinition vor. Der Herztod basiert darauf, dass Individualtod des Menschen durch den $endg{\ddot{u}}ltigen$, nicht behebbaren Stillstands von Herzschlag nach Ausfall von Atmung eingetreten sind. Auch der medizinische Laie kann den Herztod einfach wahrnehmen und sicher feststellen. Darauf folgt der Herztod als sicheres Todeszeichen angesehen wird. In der Regel trete der Herztod letzte Mal im $nat{\ddot{u}}rlichen$ Sterbeprozess ein. Die $j{\ddot{u}}ngste$ $k{\ddot{u}}nstlichen$ Reanimationsma ${\SS}nahmen$ $erm{\ddot{o}}glicht$ es, die Atmungs- und Kreislauffunktion $f{\ddot{u}}r$ eine begrenzte Zeit $k{\ddot{u}}nstlich$ aufrecht zu erhalten. Die Entwicklung der Medizin machte es zu erkennen, dass das "Zentralorgan Gehirn" $f{\ddot{u}}r$ die Aufrechterhaltung der biologischen Lebensfunktion des Gesamtorganismus unverzichtbar ist. Die Verbesserung von Transplantationstechniken gab $gro{\SS}en$ Anlass neben $f{\ddot{u}}r$ den verbesserten Reanimationstechniken auch $f{\ddot{u}}r$ den Fortschritt auf dem Gebiet der toten Transplantation. Je frischer die Organe sind, desto $gr\ddot{o}{\SS}er$ sind die Chancen $f{\ddot{u}}r$ eine erfolgreiche Verpflanzung und $f{\ddot{u}}r$ $T{\ddot{a}}tigkeit$ im $Empf{\ddot{a}}ngerorganismus$. Die Organentnahme als solche wird $unzul{\ddot{a}}ssig$ nur durch Feststellung des Herztod bei lebenswichtigen Organe, besonders wie Herz. Der Herzstillstand allein ist aber kein sicheres Kriterimn $f{\ddot{u}}r$ die $Zul{\ddot{a}}ssigkeit$ der Organentnahme. Im Korea wurde das Gesetz ${\ddot{u}}ber$ die Transplantation von Organen (Gesetz-Nummer 5858) am 8. Februar 1999 ${\ddot{o}}ffentlich$ bekannt gemacht l1nd trat am 9. Febrllar 2000 in Kraft(${\S}$ 1 Schussvorschriften kTPG). Aus der Sicht des Gesamthirntod definiert das Transplantationsgesetz derjenige, dass nach Kriterien und Nachweisverfahren $f{\ddot{u}}r$ Hirntodfeststellung dieses Gesetzes der irreversible Ausfall der Gesamthirnfunktion festgestellt wird, als Hirntoter (${\S}$ 3 Nr.4 kTPG). Mit dieser Regelung wird die Feststellung des Gesamthimtodes als gesetzliche Mindestvoraussetzung $f{\ddot{u}}r$ die Organentnahme festgelegt. Es handelt sich um Kriterien und Nachweisverfahren $f{\ddot{u}}r$ Hirntodfeststellung des koreanischen Transplantationsgesetzes.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.3
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• pp.181-191
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• 2008

Objective: Environmental chemicals alter reproduction, growth, and survival by changing the normal function of the endocrine system. Bisphenol A (BPA), one of the endocrine disruptors, is known to be an estrogen receptor agonist. Therefore, we hypothesized that BPA may affect male reproduction including spermatogenesis in the mouse testis. Methods: We used 7-week-old ICR mice. The first experiment group received BPA in sesame oil (vehicle, 1 mg/kg, 10 mg/kg, and 100 mg/kg) by i.p. injection and mice were sacrificed 24 hr later. The second experiment group received BPA (vehicle, 10 ${\mu}g/kg$, 1 mg/kg, and 100 mg/kg) daily for 14 days by subcutaneous injection. Expression of cell type-specific marker genes in the testis was evaluated by RT-PCR. Histological analysis, immunofluorescence staining, and TUNEL staining were also performed. Results: RT-PCR analyses showed that expression of luteinizing hormone receptor (LHR), a marker gene for the Leydig cell, was notably decreased in the testes of high dose-exposed mice. No obvious difference in the histology of testes was noted among treatment groups. Immunostaining of LHR in the first experiment group did not show noticeable difference in LHR protein expression in Leydig cells. Immunohistochemistry also revealed heightened expression of the immunoreactive Bax in the treatment group, and this was accompanied by positive TUNEL staining in the interstitial area within testis where Leydig cells reside. Conclusions: Our result suggests that BPA affects Leydig cell functions by altering gene expression and by increasing apoptosis in the mouse testis.

• Journal of Soil and Groundwater Environment
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• v.9 no.4
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• pp.8-14
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• 2004

To investigate the optimal operation factors in appling Soil Flushing Process, which use EDTA and boric acid composite as a flushing reagent, to the Cd(cadmium) and Cr(chromium) contaminated sites, Cd and Cr emediation rate was investigated at various experimental conditions; as EDTA concentration was increased from 0.001M to 0.1M, Cd remediation rate was increased from $73.2\%\;to\;98.5\%$; as boric acid concentration was increased from 0.001M to 0.1M, Cr remediation rate was increased from $59.2\%\;to\;99.0\%$. In the experiments using 0.005M of EDTA and 0.005M of boric acid composite as a flushing reagent at different pH regimes, Cd remediation rate was decreased from $92.4\%\;to;80.9\%$ as the pH was increased from 3 to 7. But Cr remediation rate was more effective ($70.4\%$) at pH 5 compared to the other pHs. Furthermore, at pH 5, Cd and Cr remediation rate was investigated at different molecular ratio of [EDTA]/[Boric aicd]; the optimized [EDTA]/[Boric acid] ratio was 0.01M/0.1M for Cd contaminated site and, as the concentration of boric acid was increased over 0.1M, Cr remediation rate was also increased. So the similar results were shown in the experiments using real contaminated soils which were sampled from a mining and a industrial area.

• Journal of Korean Philosophical Society
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• v.123
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• pp.27-56
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• 2012

Das Ziel dieser Abhandlung ist die Unterscheidung zwischen der kognitiven und ontischen Ununterscheidbarkeit. Jene zeigt sich als die Ununterscheidbarkeit, die aus dem Fehler des Identifizierens erfolgt. Diese besteht in der $Selbstidentit{\ddot{a}}t$ jedes individuellen Seienden. Um der Untersuchung der zwei Arten der Ununterscheidbarkeit willen soll die $Identit{\ddot{a}}t$ als Begrifflichkeit von dem Identifizieren als der subjektiven $T{\ddot{a}}tigkeit$ unterschieden werden. $W{\ddot{a}}hrend$ das kognitiv Ununterscheidbare nicht immer das Identische sein kann, ist das ontisch Ununterscheidbare notwendig das Identische. Durch diese Unterscheidung zwischen den beiden Konzeptionen der Ununterscheidbarkeit kann der Sinn des Leibniz'schen Satzes der $Identit{\ddot{a}}t$ des Ununterscheidbaren (principium identitatisindiscernibilium) deutlicher erhellt werden, indem diese Unterscheidung die kritische $Verst{\ddot{a}}rkung$ seines Satzes impliziert. Die Denkbarkeit der Duplikate, die intrinsisch $v{\ddot{o}}llig$ identisch qualifiziert sind, gilt als der bedeutsamste Einwand gegen seinen Satz der $Identit{\ddot{a}}t$. Aber der $Identit{\ddot{a}}tssatz$ - principium identitatis indiscernibilium - ist noch $g{\ddot{u}}ltig$, weil diese Duplikate letztendlich mittels der raumzeitlichen Relationsbeschaffenheit unterscheidbar sind. Hierdurch wird die grundlegende bzw. unentbehrliche Funktion der $Subjektivit{\ddot{a}}t$, die die raumzeitliche Relationsbeschaffenheit $erm{\ddot{o}}glicht$, $erl{\ddot{a}}utert$. $Daf{\ddot{u}}r$ wird Hegels $Erkl{\ddot{a}}rung$ vom $Identit{\ddot{a}}tsbegriff$ und Leibniz bearbeitet.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.335-343
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• 2022

BACKGROUND: Antibiotics used in animal husbandry for disease prevention and treatment have resulted in the rapid progression of antibiotic resistant bacteria which can be introduced into the environment through livestock feces/manure, disseminating antibiotic resistant genes (ARGs). In this study, fecal samples were collected from the livestock farms located in Jeju Island to investigate the relationship between microbial communities and ARGs. METHODS AND RESULTS: Illumina MiSeq sequencing was applied to characterize microbial communities within each fecal sample. Using quantitative PCR (qPCR), ten ARGs encoding tetracycline resistance (tetB, tetM), sulfonamide resistance (sul1, sul2), fluoroquinolone resistance (qnrD, qnrS), fluoroquinolone and aminoglycoside resistance (aac(6')-Ib), beta-lactam resistance (bla_TEM, bla_CTX-M), macrolide resistance (ermC), a class 1 integronsintegrase gene (intI1), and a class 2 integrons-integrase gene (intI2) were quantified. The results showed that Firmicutes and Bacteroidetes were dominant in human, cow, horse, and pig groups, while Firmicutes and Actinobacteria were dominant in chicken group. Among ARGs, tetM was detected with the highest number of copies, followed by sul1 and sul2. Most of the genera belonging to Firmicutes showed positive correlations with ARGs and integron genes. There were 97, 34, 31, 25, and 22 genera in chicken, cow, pig, human, and horse respectively which showed positive correlations with ARGs and integron genes. In network analysis, we identified diversity of microbial communities which correlated with ARGs and integron genes. CONCLUSION(S): In this study, antibiotic resistance patterns in human and livestock fecal samples were identified. The abundance of ARGs and integron genes detected in the samples were associated with the amount of antibiotics commonly used for human and livestocks. We found diverse microbial communities associated with antibiotics resistance genes in different hosts, suggesting that antibiotics resistance can disseminate across environments through various routes. Identifying the routes of ARG dissemination in the environment would be the first step to overcome the challenge of antibiotic resistance in the future.

• Analytical Science and Technology
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• v.22 no.1
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• pp.44-50
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• 2009

In this study, the use of purge & trap GC-MS technique for determination of methylmercury in sediment samples was described. The method detection limit of the method was determined as 0.06 ng/g and the recovery of the method was $102{\pm}11.4%$, with precisions better than 11.2%. The method was validated by analysis of CRMs such as ERM CC580 (estuarine sediment) and IAEA 405 (sediment). Additionally, the performance of the method was tested on river sediment samples and the analytical results were compared with those of the GC-CVAFS, which has been widely used for methylmercury analysis.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.9
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• pp.1035-1043
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• 2007

A leachate containing an elevated concentration of organic and inorganic compounds has the potential to contaminate adjacent soils and groundwater as well as downgradient areas of the watershed. Moreover high-strength ammonium concentrations in leachate can be toxic to aquatic ecological systems as well as consuming dissolved oxygen, due to ammonium oxidation, and thereby causing eutrophication of the watershed. In response to these concerns landfill stabilization and leachate treatment are required to reduce contaminant loading sand minimize effects on the environment. Compared with other treatment technologies, leachate recirculation technology is most effective for the pre-treatment of leachate and the acceleration of waste stabilization processes in a landfill. However, leachate recirculation that accelerates the decomposition of readily degradable organic matter might also be generating high-strength ammonium in the leachate. Since most landfill leachate having high concentrations of nitrogen also contain insufficient quantities of the organic carbon required for complete denitrification, we combined a shortcut biological nitrogen removal (SBNR) technology in order to solve the problem associated with the inability to denitrify the oxidized ammonium due to the lack of carbon sources. The accumulation of nitrite was successfully achieved at a 0.8 ratio of $NO_2^{-}-N/NO_x-N$ in an on-site reactor of the sequencing batch reactor (SBR) type that had operated for six hours in an aeration phase. The $NO_x$-N ratio in leachate produced following SBR treatment was reduced in the landfill and the denitrification mechanism is implied sulfur-based autotrophic denitrification and/or heterotrophic denitrification. The combined leachate recirculation with SBNR proved an effective technology for landfill stabilization and nitrogen removal in leachate.