• 한국축산식품학회지
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• 제36권3호
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• pp.352-358
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• 2016

The aim of this study was to determine the prevalence of enterococci in cheese samples and to characterize their antimicrobial resistance profiles as well as the associated resistance genes. A total of 139 enterococci were isolated from 99 cheese samples, the isolates were identified as E. faecalis (61.2%), E. faecium (15.1%), E. gallinarum (12.9%), E. durans (5.0%), E. casseliflavis (2.9%) and E. avium (2.9%). The most frequent antimicrobial resistance observed in enterococci isolates was to lincomycin (88.5%), followed by kanamycin (84.2%), gentamycin (low level, 51.1%), rifampin (46.8%) and tetracycline (33.8%). Among the isolates, the frequencies of high level gentamycin and streptomycin resistant enterococci strains were 2.2% and 5.8%, respectively. Apart from the mentioned antibiotics, low levels of resistance to ciprofloxacin, erythromycin and chloramphenicol were found. Moreover no resistance was observed against penicillin and ampicillin. The antimicrobial resistance genes including tetM, tetL, ermB, cat, aph(3’)-IIIa, ant(6)-Ia and aac(6’)-Ieaph(2”)-Ia were found in enterococci from Turkish cheese samples. In the current study, we provided data for antibiotic resistance and the occurrence of resistance genes among enterococci. Regulatory and quality control programs for milk and other dairy products from farms to retail outlets has to be established and strengthened to monitor trends in antimicrobial resistance among emerging food borne pathogens in Turkey.

• 동의신경정신과학회지
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• 제20권3호
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• pp.1-13
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• 2009

Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.

• 항공우주정책ㆍ법학회지
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• 제6권
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• pp.239-266
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• 1994

In den 30er Jahren fing man an, sich Gedanken $\ddot{u}ber$ eine internationale Regelung der Rechtsstellung des Luftfahrtpersonals zu machen, um Konflikte zwischen den verschiedenen nationalen Gesetzgebungen hinsichtlich der Handlungen des Luftfahrtpersonals bei der Aus bung seiner Verrichtungen zu verhindern. Aber bisher gibt es keine ausreichende Erfolg. Aus dem Vorhergehenden ergibt sich, $da{\beta}$ eine internationale Regelung der Rechtsstellung des Personals der Zivilluftfahrt erw nscht $w\ddot{a}re$. In der Praxis wird dies jedoch wegen der verschiedenen Arbeitsgesetzgebungen und der anderen nationalen Bestimmungen schwer durchzuf hren sein. Weil aber das Luftfahrtpersonal, insbesondere das Flugpersonal, eine arbeits - und sozialrechtliche Sonderstellung einnimmt, $w\ddot{a}re$ es zu empfehlen, allgemeine internationale Richtlinie zu schaffen, welche in den nationalen $Gesamtarbeitsvertr\ddot{a}gen$ $n\ddot{a}her$ ausgearbeitet werden $k\hat{o}nnten$, sowohl $f\ddot{u}r$ das Kabinenpersonal als $f\ddot{u}r$ die Piloten. Ferner $d\ddot{u}rfte$ es dringend notwendig sein, die Haftpflicht des Kommandanten ausf hrlicher und einheitlicher zu regeln, weil die Intensivierung des Lufttransports und die Anzahl der Flugg ste $st\ddot{a}ndig$ zunehmen. Hinsichtlich der Befugnisse des Kommandanten im Faile von an Bord des Luftfahrzeuges begangenen strafbaren Handlungen gibt das Abkommen von Tokio einige Bestimmungen. Die zivilrechtlichen Aspekte seiner Stellung sind noch nicht festgelegt. Es $k\ddot{o}nnte$ bei internationalen Regelung eine Vorschrift aufgenommen werden, die es $erm\ddot{o}glicht$, einem anderen als dem Kommandanten aile Vollmachten zu erteilen. $Au{\beta}erdem$ $k\ddot{o}nnten$ Regelungen geschaffen werden, welche die Rolle der $\ddot{u}brigen$ Besatzung bei der $Unterst\ddot{u}zung$ des Kommandanten $n\ddot{a}her$ umschreiben. $K\hat{o}nnte$ eine internationale Regelung in diesen Punkten erzielt werden, so $w\ddot{a}re$ sowohl der Einheitlichkeit der Rechtsvorschriften als auch der Rechtssicherheit gedient.

• 한국수정란이식학회지
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• 제1권1호
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• pp.35-49
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• 1986

Recipients are an integral part of embryo transfer and they are expensive to maintain as a good recipient. Recipient management is one of the most important components in a successful embryo transfer program. Management includes selection and subsequent care of the animals. A good recipient is basically on "open" cows or heffers whose reproductive tract is capable of receiving one or two embryos and incubating it to term. Potential recipients should be always be healthy and cycling normally ranging from 18 to 23 days. A thorough veterinary examination is recommended for candidate of recipients and cattle for questionable health should be eliminated from the recipient herd. Age and size of recipients are particularly important considerations when heifers are used, because of most embryos available for transfer are from large dams and sires. Body condition can influence a recipient's production, reproduction and health. Obese and underconditioned cattle should be avoided for use. Transfer of fresh embryos especially requires precise synchronization of donors and recipients. For estrus synchronization, PGF$_2$$\alpha$ is injected twice 10 to 12 days apart and short4erm progestagen treatment is applied to potential recipient cattle by coil into vagina (PRID) or ear implant (Synchro-Mate-B). The highest pregnancy results are achieved in recipients at exact synchrony with donors or 12 to 24 hr earlier than donors. Estrus detection is a major factor in breeding efficiency. High accuracy can be achieved by use of heat mount detection alds or by obserbing cattle for 30-minute peroids 3 times daily. Assay progesterone in milk can be used to discrIminate between pregnant and nonprenant recipients. Rectal palpation on day 35 to 70 after is an accurate and safe method of pregnancy diagnosis. Embryonic mortality in recipients may be associated with factors such as high environmental temperature and nutritional or lactational stress in early lactation period. Achievement of short calving interval requires concentrated management activity during the first 90 days following calving. Acceptable candidate for a recipient should be routinely vaccinated for infectious diseases. Proper nutritional programs according to NRC requirements and body condition scoring system for recipient cattles are vital to the ultimate success of an embryo transfer program.r program.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.132-137
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• 2010

The skc gene encoding streptokinase (SK) with a molecular mass of approximately 47.4 kDa was cloned from Streptococcus equisimilis ATCC 9542 and heterologously overexpressed in Streptomyces lividans TK24 and E. coli using various strong promoters. When the promoter for sprT [Streptomyces griseus trypsin (SGT)] was used in the host S. lividans TK24, a 47.4-kDa protein was detected along with a smaller hydrolyzed protein (44 kDa), suggesting that posttranslational hydrolysis had occurred as has been reported in other expression systems. The casein/plasminogen plate assay revealed that the plasmid construct containing the SGT signal peptide was superior to that containing the SK signal peptide in terms of SK production. Maximal production of SK was calculated to be about 0.25 unit/ml of culture broth, a value that was five times higher than that obtained with other expression systems using ermE and tipA promoters in the same host. When the skc gene was expressed in E. coli BL21(${\Delta}DE3$)pLys under the control of the T7 promoter, a relatively large amount of SK was expressed in soluble form without hydrolysis. SK activity in E. coli/pET28a-$T7_pSK_m$ was more than 2 units/ml of culture broth, even though about half of the expressed protein formed an inactive inclusion body.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9805-9812
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• 2014

Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin- suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

• 한국미생물·생명공학회지
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• 제48권3호
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• pp.296-302
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• 2020

Rapamycin, derived from Streptomyces rapamycinicus, is an important bioactive compound having a therapeutic value in managing Parkinson's disease, rheumatoid arthritis, cancer, and AIDS. Because of its pharmaceutical activity, studies over the past decade have focused on the biosynthesis of rapamycin to enhance its yield. In this study, the effect of rapG on rapamycin production was investigated. The rapG expression vector was constructed by utilizing the integration vector pSET152 under the control of the erythromycin resistance gene (ermE^⁎), a strong constitutive promoter. The rapamycin yield of wild type (WT) and WT/rapG overexpression mutant strains, under fermentation conditions, was analyzed by high-performance liquid chromatography (HPLC). Our results revealed that overexpression of rapG increased rapamycin production by approximately 4.9-fold (211.4 mg/l) in MD1 containing 15 g/l of glycerol, compared to that of the WT strain. It was also found that Illicium verum powder (10 g/l), containing shikimic acid, enhanced rapamycin production in both WT and WT/rapG strains. Moreover, the amount of rapamycin produced by the WT/rapG strain was statistically higher than that produced by the WT strain. In conclusion, the addition 15 g/l glycerol and 15 g/l I. verum powder produced the optimal conditions for rapamycin production by WT and WT/rapG strains.

• 정보관리학회지
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• 제23권3호
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• pp.241-264
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• 2006

이 연구에서는 도서관에서 구독하는 전자자원의 효율적 관리를 위한 필수 전자자원 관리용 메타데이터를 제안하였다. 제안의 근거를 확보하기 위한 기초자료로 DLF ERMI 권고안을 비롯하여 해당 권고안을 제안한 연구컨소시엄에 참여한 UCLA 대학도서관, 노스캐롤라이나주립 대학도서관, Boston대학도서관의 전자자원 관리시스템의 메타데이터의 요소를 활용하였다. 이상의 기초자료를 비교, 분석하여 도출된 결과를 국내 전자자원관리담당자에 검증을 받았다. 그 결과 본 연구에서는 다음과 같이 전자자원 관리용 메타데이터 요소를 제안하였다. 트라이얼 법주는 트라이얼 URL을 포함한 총 12개 요소, 컨소시엄 법주는 컨소시엄 명을 포함한 총 15개 요소, 수서정보 범주는 부서 ID를 포함한 총 24개 요소, 라이선스 범주는 라이선서를 포함한 총 33개 요소, 전자자원 정보는 P-ISSN을 포함한 총 21개 요소, 접근/관리정보는 관리자ID를 포함한 총 20개 요소, 이용통계 범주는 이용통계 유형을 포함한 충 13개 요소, 워크플로우 범주는 워크플로우ID를 포함한 총 14개 요소, 연락정보 범주는 연락ID를 포함한 총 18개 요소이다.

• 정보처리학회논문지D
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• 제14D권3호
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• pp.303-310
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• 2007

정보시스템 장애는 단순한 소프트웨어 오류에서부터 프로그램 시험 미비, 물리적인 방재시설 미비 등 다양하다. 비록 장애가 사소하더라도 그 결과는 엄청난 피해를 야기 시키기도 한다. 최근에는 이런 문제를 해결하는데 있어 단순한 외부 보안시스템만으로는 어렵게 되었다. 이제는 기업의 전반적인 정보기술에 대해 위험과 관련된 종합적인 대응책 수립과 적절한 대응이 필요한 시점에 도래하였다. 이와 관련하여 본 논문에서는 조직체 내에서 IT자원에 대한 무결성, 가용성, 기밀성을 고려하여 조직 내부와 외부에서 발생할 수 있는 각종 정보 및 시스템과 데이터에 대한 통제는 물론이고 위험에도 대처 할 수 있는 종합적인 위험관리시스템을 모델링하기 위한 특성에 대해 연구하였다.

• 수산해양교육연구
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• 제28권1호
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• pp.59-68
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• 2016

For comparison of tetracycline-resistant ($Tc^R$) genes, we obtained 21 and 14 $Tc^R$ Staphylococcus spp. from marine environment and human patient, respectively. Although all isolates from human were identified as Staphylococcus aureus, higher proportion of $Tc^R$ isolates (12 out of 14) from human were utilizing tet(M) gene compared to that of $Tc^R$ isolates (6 out of 21) from marine environment. Additionally, collaborated utilization of tet(M) and erm(A) in $Tc^R-Em^R$ S. aureus in human patient, but not in $Tc^R$ Staphylococcus spp. isolates from marine environment was also characterized. Based on the nucleotide sequence of transposon related to $Tc^R$ gene, we confirmed the origin of tet(M) gene in $Tc^R$ Staphylococci isolated from marine environments and human are derived from Tn916/1545-like and Tn5801 transposon, respectively. It is the first report showing the presence of Tn5801 in all $Tc^R$ S. aureus carrying tet(M) in human patient. Alignment of the fully sequenced tet(M) from marine environmental isolates was also agreed with the determined transposons by showing the genomic mosaic structure composed with three genomic parts from Tn916/1545 and unknown transposons. Genetic characteristics of these tet(M) in environmental isolates were similar to each other but different from those in isolates from human showing only tet(M) from Tn916/5801 type. It may imply the presence of less dramatic communication of antibiotic resistant genes between Staphylococci isolated from marine environment and human.