• Korean Journal of Plant Resources
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• v.25 no.4
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• pp.433-446
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• 2012

The preliminary study for providing the relationship of the factors which are climate and the pteridophyta was carried out. Dormancy form and leaf phenology of the 321 taxa belonging to 27 families were analyzed. This survey shows life-form spectrum of Korean ferns. The results obtained from this study were as follows: Deciduous (d), Evergreen (e) and Summer-deciduous (sd) were calculated as 37.4%, 59.2%, 1.9%, respectively and the Evergreen (e) has the highest value compared to the other leaf phenologies. It observed from the life-form spectra that Hemicryptophytes (H) was the most common dormancy form at 42.1%. Geophytes (G), Epiphytes (E) and Chamaephytes (Ch) were calculated at 25.5%, 15.9%, and 11.8%, respectively. There will be a discussion about geographical distribution of Korean ferns and possibility of their distribution range.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.14 no.1
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• pp.56-62
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• 2009

Stable carbon isotope analysis was used to investigate the autotrophic carbon sources supporting fisheries in three coastal bay systems of the southern coast of Korea. Carbon isotope ratios (${\delta}^{13}C$) of 31 fish species were analysed and compared with those of a range of potential primary food sources [e.g., seagrass (Zostera marina), seagrass epiphytes, benthic microalgae, macroalgae, marine particulate organic matter (marine POM), marsh plant (Phragmites australis) and terrestrial POM]. ${\delta}^{13}C$ values (range, -16.2${\sim}$-8.3‰) of fishes from the coastal embayment systems were overlapped with those of seagrass (-8.3${\pm}$1.9‰), seagrass epiphytes (-12.4${\pm}$0.6‰), benthic microalgae (-15.4${\pm}$1.6‰) and macroalgae (-16.0${\pm}$1.8‰). In addition, fishes (-12.9${\pm}$1.5‰) from the study area had distinctly higher ${\delta}^{13}C$ values compared to those collected in offshore sites (-17.3${\pm}$0.8‰) of the southern sea of Korea and Nakdong River (-23.2${\pm}$1.6‰). This result indicates that carbon supporting fish communities of these coastal bay systems is mainly derived from the local benthic producers.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.247-251
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• 2004

We developed a polyclonal antibody (PAh) based- ELISA system to accurately and rapidly monitor inocula on plant surface before onset of anthracnose. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides was determined by using indirect ELISA. It was high enough to be detectable up to ${\times}$ 12,800 dilutions. Absorbance readings exceeded (1.5even at a 10$^{-5}$ dilution. Sensitivity of PAb was precise enough to detect spore concentration as low as 50 conidia/well by indirect ELISA. PAb1 and PAb2 proved to be very sensitive and highly specific to the target pathogen, C. gloeosporioides, apparently discriminating other unrelated pathogens, or epiphytes. Absorbance values for original isolate exceeded 1.0, but no reaction was detected with other isolates, except three other anthracnose fungi: C. gloeosporioides (pepper strain), Glomerella cingulata (apple strain) and C. lagenarium. Our data suggest that PAb1 and PAb2 bind with the protein epitope that partially contains residues of amino acid, arginine, and Iysine. This kit fulfills the require-ments for detecting inoculums before infection and during onset of anthracnose on sweet persimmon.

• The Plant Pathology Journal
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• v.24 no.3
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• pp.283-288
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• 2008

Plant leaf surface is an important niche for diverse epiphytic microbes, including bacteria and fungi. Plant leaf surface plays a critical frontline defense against pathogen infections. The objective of our study was to evaluate the effectiveness of a starch-based super-absorbent polymer(SAP) combination, which enhances water potential and nutrient availability to plant leaves. We evaluated the effect of SAP on the maintenance of bacterial populations. In order to monitor bacterial populations in situ, a SAP mixture containing Pseudomonas syringae pv. tabaci that expressed recombinant green fluorescent protein(GFPuv) was spray-challenged onto whole leaves of Nicotiana benthamiana. The SAP combination treatment enhanced bacterial robustness, as indicated by disease severity and incidence. Unexpectedly, bacterial numbers were not significantly different between leaves treated with the SAP combination and those treated with water alone. Furthermore, young leaves treated with the SAP combination had more severe symptoms and a greater number of bacterial spots caused by primary and secondary infections compared to young leaves treated with the water control. In contrast, bacterial cell numbers did not statistically differ between the two groups, which indicated that measurement of viable GFP-based bacterial spots may provide a more sensitive methodology for assessing virulence of bacterial pathogens than methods that require dilution plating following maceration of bacterial-inoculated leaf tissue. Our study suggests that the SAP combination successfully increased bacterial aggressiveness, which could either be used to promote the ability of biological agents to control weedy plants or increase the robustness of saprophytic epiphytes against competition from potentially harmful microbes.

• ALGAE
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• v.29 no.4
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• pp.249-265
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• 2014

As with land crops, cultivated algae are affected by various diseases ranging from large outbreaks of a disease to chronic epiphytes, which may downgrade the value of the final product. The recent development of intensive and dense mariculture practices has enabled some new diseases to spread much faster than before. A new disease is reported almost every year, and the impact of diseases is expected to increase with environmental change, such as global warming. We observed the incidence of diseases in two Pyropia sea farms in Korea from 2011 to 2014, and estimated the economic loss caused by each disease. Serious damage is caused by the oomycete pathogens, Pythium porphyrae and Olpidiopsis spp., which decreased the productivity of the Pyropia sea farms. In Seocheon sea farms, an outbreak of Olpidiopsis spp. disease resulted in approximately US $1.6 million in loss, representing approximately 24.5% of total sales during the 2012-2013 season. The damage caused by green-spot disease was almost as serious as oomycete diseases. An outbreak of green-spot disease in the Seocheon sea farms resulted in approximately US $1.1 million in loss, representing 10.7% of total sales in the 2013-2014 season in this area. However, the causative agent of green-spot disease is still not confirmed. "Diatom felt" is regarded as a minor nuisance that does not cause serious damage in Pyropia; however, our case study showed that the economic loss caused by "diatom felt" might be as serious as that of oomycete diseases. Bacteria and cyanobacteria are indigenous members of epiphytic microbial community on Pyropia blades, but can become opportunistic pathogens under suitable environmental conditions, especially when Pyropia suffers from other diseases. A regular acid wash of the Pyropia cultivation nets is the most common treatment for all of the above mentioned diseases, and represents approximately 30% of the total cost in Pyropia sea farming. However, the acid wash is ineffective for some diseases, especially for Olpidiopsis and bacterial diseases.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.252-257
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• 2004

We developed a polyclonal antibody based-ELISA system to monitor inocula accurately and rapidly before onset of anthracnose on soybean sprouts. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides, determined by indirect ELISA, was high enough to be detectable up to ${\times}$25,600 dilutions. Both PAb1 and PAb2 had the highest level of reactivity to Colletotrichum gloeosporioides. Absorbance readings exceeded 0.15. Sensitivity of PAb to C. gloeosporioides was precise enough to detect spore concentration as low as 500 conidia/well by indirect ELISA. Both antibodies are very sensitive and highly specific to the target pathogen Colletotrichum gloeosporioides, apparently discriminating other unrelated pathogen, or epiphytes. This kit fulfills the requirements far detecting inocula before infection and onset of anthracnose. Our ELISA system should also be feasible to detect C. acutatum (Mungbean sprouts rot) and G. cingulata (C. gleosporioides), (apple, pepper). It was remarkable that absorbance value was not reduced even after 4 consecutive washings (Fig.4), suggesting that antigenic determinants are on the surface of conidia. Antigenic determinant was characterized by heating and enzyme treatment: Both PAb1 and PAb2 bind to protein epitope that does not contain residue of amino acid, arginine, and Iysine, even though more work needs to be done.

• 한국해양학회지
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• v.21 no.1
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• pp.25-33
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• 1986

Ontogenetic food habits of the four most abundant fish species in seagrass neadows of Redfish Bay, Texas, were examined quantitatively during 1982-1983. The darter goby (Gobionellus bolelsoma) and pinfish (Lagodon rhomboides) were trophic generalists, which used a wide range of food items. The darter goby had relatively diverse, omnivorous diet which included amphipods, copepods, polychaetes, filamentous algae, diatoms, and detritus. This species did not show distinct ontogenetic changes in foodpreferences. Unlike the darter goby, the pinfish showed ontogenetic progression of four feeding stages. An initial feeding stage was a planktivorous stage in which copepods were int major food items, followed by a carnivorous stage in which amphipods became the major food items, an omnivorous stage in which filamentous algae, diatoms, amphipods, and polychaetes were the major food items, and finally a herbivorous stage in which seagrass pieces with attached epiphytes and their debris were the major food items. The code goby (Gobiosoma robustrm) and Gulf pipefish (Syngathus scovelli) appeared to be relatively specialized in food havits as carnivorous. Similar ontogenetic changes in food habits were observed for these two species, i.e.initially, copepods were the major food items, followed by a gradual transition to amphipods with growth.

• Korean Journal of Environment and Ecology
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• v.27 no.5
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• pp.529-543
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• 2013

In this study, Pteridophytes flora of Jirisan National Park were recorded 135 taxa, and they belong to 21 families, 39 genera, 123 species, 11 varieties and 1 forma. In the field, 38 taxa were not found on literature exists and 22 taxa were newly confirmed. The results obtained from this study were as follows: Deciduous (d), Evergreen (e) and Summer-deciduous (sd) were calculated as 50.0%, 46.3%, 2.2%, respectively and the Deciduous (d) has the highest value compared to the other leaf phenologies. It observed from the life-form spectra that Hemicryptophytes (H) was the most common dormancy form at 44.8%. Geophytes (G), Epiphytes (E), Hydatophytes (HH), Therophytes (Th) and Chamaephytes (Ch) were calculated at 26.1%, 10.5%, 4.5%, 0.8% and 13.4%, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.419-427
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• 2023

This study, analyzed the stomach contents, stable isotope signatures (δ¹³C and δ¹⁵N) in muscle tissue, and potential food sources (particulate organic matter, Zostera marina, epiphytes, and zooplankton) of Sardinops melanostictus in the southern coast of Korea to evaluate its dietary changes across seasons (summer and autumn 2022). The diet of S. melanostictus predominantly comprised organic detritus (99%) regardless of the season. The isotope results showed that S. melanostictus had mean δ¹³C and δ¹⁵N values of -17.7±0.4‰ and 10.6±0.5‰, respectively and seasonal variations were observed (t-test, P=0.010, P=0.002). However, the isotopic mixing model showed that particulate organic matter was a major nutritional source for S. melanostictus in summer and autumn (88.7% and 90.2%, respectively). Based on the stomach contents and stable isotope signatures, these results may help improve the understanding of S. melanostictus and establish the management measures for their conservation.

• Korean Journal of Plant Taxonomy
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• v.44 no.3
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• pp.222-232
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• 2014

The flora of vascular plants in Sumeunmulbaengdui wetland located in Hallasan National Park was surveyed 9 times from September, 2010 to May, 2014. This survey resulted in 236 taxa in total composed of 202 species, 2 subspecies, 27 varieties and 5 forms of 148 genera under 69 families. They were categorized by their life forms into 8 groups of 37 megaphanerophytes, 26 nanophanerophytes, 2 epiphytes, 3 chamaephytes, 84 hemicryptophytes, 33 geophytes, 21 hydrophytes, 30 therophytes. Among them, 10 taxa of Korean endemic plants and 9 taxa of rare and endangered plants were included. In addition, the floristic regional indicator plants were 43 taxa comprised of 3 taxa of grade V, 7 taxa of grade IV, 12 taxa of grade III, 8 taxa of grade II and 13 taxa of grade I. 2 species of alien plants, Erechtites hieracifolia Raf. and Taraxacum officinale Weber were found. Sustainable interest and protective measures to Sumeunmulbaengdui wetland are required, since this area is comparatively larger in size and higher in plant diversity than other Ramsar wetlands in Jeju-do Province.