• Title/Summary/Keyword: enzyme immobolization

Search Result 1, Processing Time 0.014 seconds

Potentimetric Biosensor for Detection of L-Malate and D-Isocitrate Employing ${CO_{3}}^2-$ -Selective Electrode and Enzyme Immobilization in Flow Injection Analysis

  • Kwun, In-Sook;Kim, Meera
    • Preventive Nutrition and Food Science
    • /
    • v.3 no.1
    • /
    • pp.36-42
    • /
    • 1998

  • Ion-selective eleltrodes(ISEs) are simple electrodechemical devices for the direct measurement of ions in the samples. A novel potentiometric biosensor for the determination of L-Malate or D-isocitrate has been developed by using CO2-3 -ISE-FIA system was composed of a pump, an injector, a malic enzyme or isocitric dehydrogenase enzyme reactor, a CO2-3 -ISE, a pH/mV meter, and an integrater. The various factors, such as buffer capacity types of plstericizer and polymer, were optimized for the CO2-3 selectivity. In this novel CO2-3 --ISE-FIA system, the potential difference due to the amount of CO2-3 produced from each enzyme reaction was proportional to the amount of L-malate or D-isocitrate.

  • PDF