• Mycobiology
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• 제31권4호
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• pp.196-199
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• 2003

A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

• 한국디지털건축인테리어학회논문집
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• 제8권2호
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• pp.47-56
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• 2008

The purpose of this study is to arrange the design elements and methods for sustainable community. After made the concept of sustainable community clear in the contemporary architecture and urban design, this study examines rural ecovillage, urban demonstration project, co-housing, new urbanism and urban village, and ecological township as the application modes of sustainable community. Through the cases of the application mode, the design elements for sustainable community were summarized as the followings: the symbiosis system with the nature, permaculture and edible landscape, identity, the technology for saving of resources and energy, the traffic system for the reduction of private motor use, mixed use development, and the housing with diversity. And the design methods for sustainable community were summarized as the followings: strategic planning, visioning, community action planning, workshops and charrette, environmental evaluation, and participation techniques.

• Journal of Microbiology and Biotechnology
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• 제4권3호
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• pp.176-182
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• 1994

The light-organ symbiont of pine cone fish, Vibrio fischeri, senses its presence in the host and responds to environmental changes by differentially expressing its symbiosis-related luminescence genes. The V. fischeri luminescence genes are activated by LuxR protein in the presence of an autoinducer. In an effort to elucidate the mechanism of regulation of luxR, a plasmid containing luxR was mutagenized in vitro with hydroxylamine and a luxR mutant plasmid was isolated by its ability to activate luminescence genes cloned in E. coli in the absence of the autoinducer. The specific base change identified by DNA sequencing was only single base transition at ＋78 from the transcriptional start of luxR. Based on a Western immunoblot analysis, the nucleotide change directed the synthesis of much higher level of LuxR protein without any amino acid substitutions. The results suggest that the region including the ＋78th base is presumably internal operator required for autorepression of luxR, and the increased cellular level of LuxR results in activation of luminescence genes by autoinducer independent fashion.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.49-49
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• 2018

A variety of bioactive secondary metabolites have been reported from plant-associated microorganisms. Halophytes, plants that can only grow in hypersaline area, were reported to host beneficial microorganisms such as plant growth-promoting endophytes. The microorganisms have been reported to show notable mutualistic symbiosis with halophytes to help them survive in high saline condition. Finding out bioactive secondary metabolites as well as elucidation of relationship(s) between microbes and the host halophyte has been paid attention, because of their functional diversity. Novel microbes often have associated with novel natural products. In an effort to investigate natural compounds with interesting structures from fungi, we selected plants from a distinct environmental setting which could be a promising source. Several fungi were isolated from halophyte or medicinal plants. Some strains of the fungi were cultivated on a large scale and extracted with ethyl acetate, which were subjected to a series of chromatographic methods, leading to the isolation of tens of compounds. The isolated compounds were identified by analysis of spectroscopic methods such as 1D-, 2D-NMR, and MS. Details of isolation, structure determination, and biological activities will be discussed.

• 한국실내디자인학회논문집
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• 제19권5호
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• pp.190-197
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• 2010

This is a study about public-regeneration-architecture design of the 3rd world damaged by the development of capital society of the 20th century and from the perspective of economically poor, anti-capitalist development from non-profit design organization. Active among such non-profit design organizations, the sustainable design characteristics of public-regeneration-architecture of the Architecture for Humanity will be discussed. I help it so as a continuous possibility design to have been given to public-regeneration-architecture of the Architecture for Humanity is small, and I establish a construction space at a few costs to people in a situation to be environmentally interior economic, to be cultural, and I am large, and to be able to generate profitable construction by environment at activation of a local community and worldwide dimensions. I look through a sustainable design way of this study classifies it to cultural sustainability that ecology-cultural approach in the environmental sustainability result ecology - central approach, and how I was applied to materials of construction, color, a pattern, a construction method, space configuration etc. There is the meaning to try to analyze a characteristic of a sustainable design to have appeared to public-regeneration-architecture of the Architecture for Humanity which I make a symbiosis, and you can live on it how in the present cities that city poor floor will add to.

• 한국환경농학회지
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• 제27권4호
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• pp.362-367
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• 2008

The legume-rhizobia symbiosis is an important source of plant growth and nitrogen fixation for many agricultural systems. This study was conducted to investigate the effects of salinity stress on nitrogen fixation and growth of pea (Pisum sativum L.), which has antimutagenic activities against chemical mutagen, inoculated with R. leguminosarum bv. viciae cultured with additional plant-to-rhizobia signal compounds, naringenin (NA,15 uM), methyl-jasmonate (MJ, 50 uM) or both, under greenhouse conditions. Three salinity levels (0.6, 3.0 and $6.0\;dS\;m^{-1}$) were imposed at 3 days after transplanting and maintained through daily irrigations. Addition of signal compounds under non-stress and stress conditions increased dry weight, nodule numbers, leaf area and leaf greenness. The inducers increased photosynthetic rate under non-stress and stress conditions, by approximately 5-20% when compared to that of the non-induced control treatment. Under stress conditions, proline content was less in plants treated with plant-to-bacteria signals than the control, but phenol content was significantly increased, compared to that of the control. The study suggested that pre-incubation of bacterial cells with plant-to-bacteria signals could enhance pea growth, photosynthesis, nitrogen fixation and biomass under salinity stress conditions.

• 가족자원경영과 정책
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• 제16권1호
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• pp.21-39
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• 2012

The purpose of this study is to propose the direction of environmental education for early childhood children based on the environment-friendly life style and to improve the possibility of application of it at home and kindergarten. This study reviewed reflectively the early studies on the concepts of ecocentrism, the emerging process of it, the importance of environmental education for early childhood children based on the ecocentrism, and the practice of these kinds of education at home and kindergarten. The main concepts connected to the environment-friendly life style based on ecocentrism, that is, 'preservation of nature', 'interdepentence', 'wholeness', 'equilibrium' are the foundations of environmental education for early childhood children. When these kinds of concepts are considered, the aims of environmental education for early childhood children should be focused on the life style that attaches great importance to the symbiosis of human being and nature. The educational contents and methods in harmony with ecocentrism are as follows: The educational methods should be connected to the education based on ordinariness, the learning through adults' model, the family-community-centered activities, and ways of thinking of the unity of knowledge and conduct. And educational contents should include all of the life styles in clothes, food, and shelter. And the educational methods also should be connected to the education through joint working of children and teacher, play-centered education, activities considering ages and individual variations, and education connected home. And educational contents should include many different kinds of activities of experiencing nature outside home and kindergarten.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.238-244
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• 2010

Rhizobia are well-known for their ability to infect and nodulate legume roots, forming a nitrogen-fixing symbiosis of agricultural importance. In addition, recent studies have shown that rhizobia can colonize roots and aerial plant tissues of rice as a model plant of the Graminaceae family. Here we show that rhizobia can invade tobacco, a model plant belonging to the Solanaceae family. Inoculation of seedling. roots with five GFP-tagged rhizobial species followed by microscopy and viable plating analyses indicated their colonization of the surface and interior of the whole vegetative plant. Blockage of ascending epiphytic migration by coating the hypocotyls with Vaseline showed that the endophytic rhizobia can exit the leaf interior through stomata and colonize the external phyllosphere habitat. These studies indicate rhizobia can colonize both below- and above-ground tissues of tobacco using a dynamic invasion process that involves both epiphytic and endophytic lifestyles.

• International Journal of Computer Science & Network Security
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• 제22권2호
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• pp.107-114
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• 2022

The main purpose of this study is to substantiate the theoretical and methodological foundations of the organizational and economic mechanism of development of the agro-industrial complex in modern conditions. Organizational and economic mechanism is presented as a complex organizational structure of the system type, which is aimed at performing specific functions, the characteristic feature of which is the constant support of process changes without which the organizational and economic mechanism can not exist. There are four components of the agro-industrial complex, represented by agriculture and the national economy, which ensure its operation, including industry, processing of agricultural products, its storage and transportation, sale and repair and maintenance of agricultural machinery and more. It is proved that the organizational and economic mechanism of development of agro-industrial complex in modern conditions it is expedient to consider: from the point of view of system and process approaches; as a set of economic levers and organizational measures to influence the agro-industrial complex; constituent components of organizational influence on the development of the complex; a set of components, elements that are integrated into the system of economic relations of the subjects of the agro-industrial complex; a set of purposeful stimulators of agro-industrial complex development. The functions of the organizational component of the mechanism of agro-industrial complex include: redistributive, planning, interaction, control, integration and regulatory functions, the functions of the economic component include consumer, investment and innovation, social, incentive, monitoring functions of the mechanism. The symbiosis of the functions of organizational and economic components ensure the effectiveness of the organizational and economic mechanism of the organizational and economic mechanism through its functionalities as a whole.

• Advances in environmental research
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• 제4권2호
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• pp.83-104
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• 2015

Air Quality Index (AQI) is a pointer to broadcast short term air quality. This paper presents one day ahead AQI forecasting on seasonal basis for three major cities in Maharashtra State, India by using Artificial Neural Networks (ANN) and Genetic Programming (GP). The meteorological observations & previous AQI from 2005-2008 are used to predict next day's AQI. It was observed that GP captures the phenomenon better than ANN and could also follow the peak values better than ANN. The overall performance of GP seems better as compared to ANN. Stochastic nature of the input parameters and the possibility of auto-correlation might have introduced time lag and subsequent errors in predictions. Spectral Analysis (SA) was used for characterization of the error introduced. Correlational dependency (serial dependency) was calculated for all 24 models prepared on seasonal basis. Particular lags (k) in all the models were removed by differencing the series, that is converting each i'th element of the series into its difference from the (i-k)"th element. New time series is generated for all seasonal models in synchronization with the original time line & evaluated using ANN and GP. The statistical analysis and comparison of GP and ANN models has been done. We have proposed a promising approach of use of GP coupled with SA for real time prediction of seasonal multicity AQI.